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The map label for this gene is gatB

Identifier: 89054357

GI number: 89054357

Start: 1852825

End: 1854336

Strand: Reverse

Name: gatB

Synonym: Jann_1866

Alternate gene names: 89054357

Gene position: 1854336-1852825 (Counterclockwise)

Preceding gene: 89054361

Following gene: 89054356

Centisome position: 42.94

GC content: 63.29

Gene sequence:

>1512_bases
ATGCTTGACCTGACCTATGACACCCCCAAACCCCGCGTGATCGCGGGCGCCAAGCAGGATTGGGAACTGGTGATCGGGAT
GGAGGTGCATGCCCAGGTCTCCACCCGCGCCAAGCTGTTCTCCGGCGCGTCCACGCGGTTCGGGGCCGAGCCCAATTCCA
ACGTCGCCTTCGTGGATGCGGGCATGCCCGGCATGTTGCCCGTGATCAACGAGGCCTGCGTGTCCCAGGCCGTGCGCACG
GGCCTTGGCCTGAAGGCGCAGATCAATCTGGAATCGGCCTTTGACCGCAAGAACTACTTCTACCCCGACCTGCCCCAGGG
CTATCAGATCAGCCAGCTCTACCATCCCATCGTGGGCGAAGGCGAAGTGCTGGTGGAACTGGGCGACGGTCTCGCGCGGC
TGGTGCGCATCGAACGCATCCATCTGGAACAGGACGCGGGCAAGAGCATCCACGACATGGACCCCGCCCTGTCCTTCGTG
GACCTCAACCGCACCGGCGTCGCCCTGATGGAGATCGTGTCGAAGCCGGACATCCGCTCGCCCGAAGAAGCCGCCGCCTA
CGTCGTGAAACTGCGCCAGATCATGCTCTATCTCGGCACCTGCGACGGCAACATGCAAAACGGCAACCTGCGCGCGGATG
TGAACGTCTCGATCTGCGCGCCCGGCCAGTACGAGAAGTATCAGGCGACGCAGGATTTTTCGCATCTGGGCACCCGGTGC
GAGATCAAGAACATGAACTCCATGCGCTTCATCCAGCAGGCGATCGAAGTGGAGGCCAAGCGCCAGATCGCCATCATTGA
AGGCGGCGGCACGGTCGATCAGGAAACCCGCCTTTATGACCCCGACAAGGGCGAGACGCGCTCCATGCGGTCCAAGGAGG
AGGCCCATGATTACCGCTACTTCCCCGACCCCGACCTTTTGCCCCTGACCTTCGATCAGGCCTATGTCGACGCGATCGCC
GCCGACCTCCCCGAACTTCCCGACGCCAAGAAGGCGCGCTTTATCAGGGGCTTCGGCCTATCGGATTACGACGCCTCCGT
CCTGACCGCCGATATCGACGCCGCGCAATATTTTGAGGCCACAGCCACCAAGGACACCGGCAAGCTGTCCGCCAACTGGG
TCATCAATGAGCTGTTCGGCCGCCTCAAGAAGGACGAGCGCCAGATCGAAGACAGCCCCGTCGCCCCCGCCCAACTGGCC
TCCCTCATCGCGCTGATCGCCTCCGACACGATCTCCGGCAAGATCGCCAAGGACGTGTTCGAGATCAGCTACACCACCGG
CCGCGACCCCTCCGAAATCGTGGAGACTGAGGGCCTCAAACAGGTCACCGACACCGGCGCGATCGAGGCTGCGGTGGATC
AGATCATCGCCGACAACCCCGCCCAGGTCGCCAAGGCCCAGGAAAACCCCAAACTCGCCGGCTGGTTCGTGGGTCAGGTG
ATGAAAGCCACCGGCGGCAAGGCCAACCCCAAGGCCGTTAACCAGATCGTCGCCCAGAAGCTCGGCGGGTAG

Upstream 100 bases:

>100_bases
GTGCTCGCCACGCACCCGACCGCCCCGGCGGAGCGGAACGCCCACTTGCCCACCCTCCGGCGCTGTCTTAAACCCCCTTT
GACCAATGCCGGAGGCTCCC

Downstream 100 bases:

>100_bases
ATGCGCCGCCGCGACCCGCTCGCCACCCATAACGAGCGGGTGAAGCTGCGCGCGCAGTTCCTCAACGCGCTCGCCATCGG
TTTTCTCGGCTTCGCCCTTC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MLDLTYDTPKPRVIAGAKQDWELVIGMEVHAQVSTRAKLFSGASTRFGAEPNSNVAFVDAGMPGMLPVINEACVSQAVRT
GLGLKAQINLESAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGLARLVRIERIHLEQDAGKSIHDMDPALSFV
DLNRTGVALMEIVSKPDIRSPEEAAAYVVKLRQIMLYLGTCDGNMQNGNLRADVNVSICAPGQYEKYQATQDFSHLGTRC
EIKNMNSMRFIQQAIEVEAKRQIAIIEGGGTVDQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLTFDQAYVDAIA
ADLPELPDAKKARFIRGFGLSDYDASVLTADIDAAQYFEATATKDTGKLSANWVINELFGRLKKDERQIEDSPVAPAQLA
SLIALIASDTISGKIAKDVFEISYTTGRDPSEIVETEGLKQVTDTGAIEAAVDQIIADNPAQVAKAQENPKLAGWFVGQV
MKATGGKANPKAVNQIVAQKLGG

Sequences:

>Translated_503_residues
MLDLTYDTPKPRVIAGAKQDWELVIGMEVHAQVSTRAKLFSGASTRFGAEPNSNVAFVDAGMPGMLPVINEACVSQAVRT
GLGLKAQINLESAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGLARLVRIERIHLEQDAGKSIHDMDPALSFV
DLNRTGVALMEIVSKPDIRSPEEAAAYVVKLRQIMLYLGTCDGNMQNGNLRADVNVSICAPGQYEKYQATQDFSHLGTRC
EIKNMNSMRFIQQAIEVEAKRQIAIIEGGGTVDQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLTFDQAYVDAIA
ADLPELPDAKKARFIRGFGLSDYDASVLTADIDAAQYFEATATKDTGKLSANWVINELFGRLKKDERQIEDSPVAPAQLA
SLIALIASDTISGKIAKDVFEISYTTGRDPSEIVETEGLKQVTDTGAIEAAVDQIIADNPAQVAKAQENPKLAGWFVGQV
MKATGGKANPKAVNQIVAQKLGG
>Mature_503_residues
MLDLTYDTPKPRVIAGAKQDWELVIGMEVHAQVSTRAKLFSGASTRFGAEPNSNVAFVDAGMPGMLPVINEACVSQAVRT
GLGLKAQINLESAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGLARLVRIERIHLEQDAGKSIHDMDPALSFV
DLNRTGVALMEIVSKPDIRSPEEAAAYVVKLRQIMLYLGTCDGNMQNGNLRADVNVSICAPGQYEKYQATQDFSHLGTRC
EIKNMNSMRFIQQAIEVEAKRQIAIIEGGGTVDQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLTFDQAYVDAIA
ADLPELPDAKKARFIRGFGLSDYDASVLTADIDAAQYFEATATKDTGKLSANWVINELFGRLKKDERQIEDSPVAPAQLA
SLIALIASDTISGKIAKDVFEISYTTGRDPSEIVETEGLKQVTDTGAIEAAVDQIIADNPAQVAKAQENPKLAGWFVGQV
MKATGGKANPKAVNQIVAQKLGG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=507, Percent_Identity=39.2504930966469, Blast_Score=354, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI115534094, Length=315, Percent_Identity=39.0476190476191, Blast_Score=210, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=436, Percent_Identity=31.651376146789, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI45550808, Length=504, Percent_Identity=34.7222222222222, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_JANSC (Q28R79)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509808.1
- ProteinModelPortal:   Q28R79
- SMR:   Q28R79
- STRING:   Q28R79
- GeneID:   3934317
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1866
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q28R79
- ProtClustDB:   PRK05477
- BioCyc:   JSP290400:JANN_1866-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54963; Mature: 54963

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLTYDTPKPRVIAGAKQDWELVIGMEVHAQVSTRAKLFSGASTRFGAEPNSNVAFVDA
CCCCCCCCCCCCEEECCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
GMPGMLPVINEACVSQAVRTGLGLKAQINLESAFDRKNYFYPDLPQGYQISQLYHPIVGE
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCC
GEVLVELGDGLARLVRIERIHLEQDAGKSIHDMDPALSFVDLNRTGVALMEIVSKPDIRS
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEECCCCHHHHHHHHCCCCCCC
PEEAAAYVVKLRQIMLYLGTCDGNMQNGNLRADVNVSICAPGQYEKYQATQDFSHLGTRC
HHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCEE
EIKNMNSMRFIQQAIEVEAKRQIAIIEGGGTVDQETRLYDPDKGETRSMRSKEEAHDYRY
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
FPDPDLLPLTFDQAYVDAIAADLPELPDAKKARFIRGFGLSDYDASVLTADIDAAQYFEA
CCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHH
TATKDTGKLSANWVINELFGRLKKDERQIEDSPVAPAQLASLIALIASDTISGKIAKDVF
HCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
EISYTTGRDPSEIVETEGLKQVTDTGAIEAAVDQIIADNPAQVAKAQENPKLAGWFVGQV
EEEECCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHH
MKATGGKANPKAVNQIVAQKLGG
HHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDLTYDTPKPRVIAGAKQDWELVIGMEVHAQVSTRAKLFSGASTRFGAEPNSNVAFVDA
CCCCCCCCCCCCEEECCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
GMPGMLPVINEACVSQAVRTGLGLKAQINLESAFDRKNYFYPDLPQGYQISQLYHPIVGE
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCC
GEVLVELGDGLARLVRIERIHLEQDAGKSIHDMDPALSFVDLNRTGVALMEIVSKPDIRS
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEECCCCHHHHHHHHCCCCCCC
PEEAAAYVVKLRQIMLYLGTCDGNMQNGNLRADVNVSICAPGQYEKYQATQDFSHLGTRC
HHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCEE
EIKNMNSMRFIQQAIEVEAKRQIAIIEGGGTVDQETRLYDPDKGETRSMRSKEEAHDYRY
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
FPDPDLLPLTFDQAYVDAIAADLPELPDAKKARFIRGFGLSDYDASVLTADIDAAQYFEA
CCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHH
TATKDTGKLSANWVINELFGRLKKDERQIEDSPVAPAQLASLIALIASDTISGKIAKDVF
HCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
EISYTTGRDPSEIVETEGLKQVTDTGAIEAAVDQIIADNPAQVAKAQENPKLAGWFVGQV
EEEECCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHH
MKATGGKANPKAVNQIVAQKLGG
HHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA