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The map label for this gene is cysG [H]

Identifier: 89054266

GI number: 89054266

Start: 1762879

End: 1764300

Strand: Reverse

Name: cysG [H]

Synonym: Jann_1775

Alternate gene names: 89054266

Gene position: 1764300-1762879 (Counterclockwise)

Preceding gene: 89054268

Following gene: 89054265

Centisome position: 40.86

GC content: 65.4

Gene sequence:

>1422_bases
ATGCGCCATTTTCCAATCTTCCTCGACCTGCGTGGTCGTCGTGTTGTGCTGGCAGGCGGTGGAGAGGCCGCGCTGGCCAA
GCTGCGGTTGATCCTGAAGTCCGAGGCGCGAGTGACGGTCTTCGCGACAGACCCCGCGCCGGAGATCGTGGCGTGGGACG
CGGAGCGCAGGCTGCGCCTTGTCAAACGCACCTTCGGCCCCGGAGATGCGCTCTGCGCGTCGCTGGCCTATGCCGCAAGC
GAAGATGAGGCAGAGGACGCCCGCGTCGCGACGCTGGCCCGCGCCGATGGGGCATTGATCAACATCGTCGATAATCTGGA
GGGCTCCGCCTTCATCACGCCCGCCATCGTTGACCGCGACCCGGTGACAATTGCCATCGGAACCGAAGGCGCCGCCCCGG
TTTTGGCCCGCAGGATCAAGGCGGATCTGGAGGCGCAATTGCCCGCCTCCCTCGGGCTTCTGGCACGCATCGGGCAAGGC
TTCCGCACGGCGGCCAACGCCCTGCCCATGGGCCGCAAGCGTCGCGATTTCTGGTCTGACTTCTACTTCAAGAAAGGCCC
CGAAGCCCTTGCCAAAGAAGGAAAACAGGGCGTTGAGCTGGCCCTTGGTGATTTGCTGATCGACCATCTCCATTGCGAGG
AGACGCCGGGCCGTGTGGATCTTGTGGGCGCAGGCCCCGGCGACCCGGACCTGCTGACGCTCAAGGCGCGCAAATTGCTC
GATCAGGCAGATGTCGTGATCTACGACCGCCTGGTTTCGCCTGAAATCCTGGAGCTCGCCCGCCGCGAGGCGACGATGGT
GAATGTGGGCAAAAAAGGCTTTGGCCCCTCCACCAAGCAGGACGATATCGCCGCCGCGATGATCGCGCACGCCAGCCAGG
GCGCCCATGTGGTGCGCCTGAAATCCGGCGATCCGGGCGTCTATGGCCGTCTGGATGAGGAAATCGACGCGCTGGACGCG
GCGGGCATAGATTGGTCCATCGTGCCGGGCATCACAGCGGCCAATGCGGCCTCGGCCCAGATCGGCCAGAGCCTGACGAA
ACGCGGGCGCAATGGCGCGATGCGGTTTCTGACCGGCCATGACGTGAAGGGCTTTGCCGAGCAGGATTGGCGCAGCCTCG
CCAAACCCGGTCAGGTCGCGGCCATCTATATGGGCGCGAAGGGCGCGCGGTTCCTGCAAGGCCGCCTGATGATGCATGGA
ATTGCGCCCGACACACCCGTCACGGCGGTGGAAAACGCCTCGCGCACCGACGCGCGGATCATCGCGTCCACCATTGCGAC
CCTGCCAATGGATCTTGACGACGCCGCCCCCGACGGCCCCGTAATCCTGATGCTTGGCCTTGCCCCACGTGCCGCCGCCG
GCGTGGATCTGCGGCGGGCGGTCGCGGGTCAAGTCCCTACTCTGCAAGAGGAATTTGCCTGA

Upstream 100 bases:

>100_bases
CGGGATACAGAACATGCGTCCCTTTCAGCAAGCCTCACCTGCCATTTATCGGGAAAATATCCTATCTTGAGGGGGCATTC
AGCAAGCGAGGTTCCATCCC

Downstream 100 bases:

>100_bases
TGCCCCGCGCGTTCACCCCCAAAGTCGTCACTGCAAATGCCCTGCTGGAAGGCGATGTCGTCTACCTCACCAAGCAAGGC
CACTGGACCCGCCTCCACGC

Product: uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MRHFPIFLDLRGRRVVLAGGGEAALAKLRLILKSEARVTVFATDPAPEIVAWDAERRLRLVKRTFGPGDALCASLAYAAS
EDEAEDARVATLARADGALINIVDNLEGSAFITPAIVDRDPVTIAIGTEGAAPVLARRIKADLEAQLPASLGLLARIGQG
FRTAANALPMGRKRRDFWSDFYFKKGPEALAKEGKQGVELALGDLLIDHLHCEETPGRVDLVGAGPGDPDLLTLKARKLL
DQADVVIYDRLVSPEILELARREATMVNVGKKGFGPSTKQDDIAAAMIAHASQGAHVVRLKSGDPGVYGRLDEEIDALDA
AGIDWSIVPGITAANAASAQIGQSLTKRGRNGAMRFLTGHDVKGFAEQDWRSLAKPGQVAAIYMGAKGARFLQGRLMMHG
IAPDTPVTAVENASRTDARIIASTIATLPMDLDDAAPDGPVILMLGLAPRAAAGVDLRRAVAGQVPTLQEEFA

Sequences:

>Translated_473_residues
MRHFPIFLDLRGRRVVLAGGGEAALAKLRLILKSEARVTVFATDPAPEIVAWDAERRLRLVKRTFGPGDALCASLAYAAS
EDEAEDARVATLARADGALINIVDNLEGSAFITPAIVDRDPVTIAIGTEGAAPVLARRIKADLEAQLPASLGLLARIGQG
FRTAANALPMGRKRRDFWSDFYFKKGPEALAKEGKQGVELALGDLLIDHLHCEETPGRVDLVGAGPGDPDLLTLKARKLL
DQADVVIYDRLVSPEILELARREATMVNVGKKGFGPSTKQDDIAAAMIAHASQGAHVVRLKSGDPGVYGRLDEEIDALDA
AGIDWSIVPGITAANAASAQIGQSLTKRGRNGAMRFLTGHDVKGFAEQDWRSLAKPGQVAAIYMGAKGARFLQGRLMMHG
IAPDTPVTAVENASRTDARIIASTIATLPMDLDDAAPDGPVILMLGLAPRAAAGVDLRRAVAGQVPTLQEEFA
>Mature_473_residues
MRHFPIFLDLRGRRVVLAGGGEAALAKLRLILKSEARVTVFATDPAPEIVAWDAERRLRLVKRTFGPGDALCASLAYAAS
EDEAEDARVATLARADGALINIVDNLEGSAFITPAIVDRDPVTIAIGTEGAAPVLARRIKADLEAQLPASLGLLARIGQG
FRTAANALPMGRKRRDFWSDFYFKKGPEALAKEGKQGVELALGDLLIDHLHCEETPGRVDLVGAGPGDPDLLTLKARKLL
DQADVVIYDRLVSPEILELARREATMVNVGKKGFGPSTKQDDIAAAMIAHASQGAHVVRLKSGDPGVYGRLDEEIDALDA
AGIDWSIVPGITAANAASAQIGQSLTKRGRNGAMRFLTGHDVKGFAEQDWRSLAKPGQVAAIYMGAKGARFLQGRLMMHG
IAPDTPVTAVENASRTDARIIASTIATLPMDLDDAAPDGPVILMLGLAPRAAAGVDLRRAVAGQVPTLQEEFA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=437, Percent_Identity=39.5881006864989, Blast_Score=279, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6322922, Length=273, Percent_Identity=27.8388278388278, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 50194; Mature: 50194

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHFPIFLDLRGRRVVLAGGGEAALAKLRLILKSEARVTVFATDPAPEIVAWDAERRLRL
CCCCCEEEEECCCEEEEECCCHHHHHHHHHHEECCCEEEEEECCCCCCEEEECHHHHHHH
VKRTFGPGDALCASLAYAASEDEAEDARVATLARADGALINIVDNLEGSAFITPAIVDRD
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCC
PVTIAIGTEGAAPVLARRIKADLEAQLPASLGLLARIGQGFRTAANALPMGRKRRDFWSD
CEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHH
FYFKKGPEALAKEGKQGVELALGDLLIDHLHCEETPGRVDLVGAGPGDPDLLTLKARKLL
HHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEHHHHHHH
DQADVVIYDRLVSPEILELARREATMVNVGKKGFGPSTKQDDIAAAMIAHASQGAHVVRL
HHCCEEEEECCCCHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
KSGDPGVYGRLDEEIDALDAAGIDWSIVPGITAANAASAQIGQSLTKRGRNGAMRFLTGH
ECCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECC
DVKGFAEQDWRSLAKPGQVAAIYMGAKGARFLQGRLMMHGIAPDTPVTAVENASRTDARI
CCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCEEECCCCCCCCHHHCCCCCCHHHH
IASTIATLPMDLDDAAPDGPVILMLGLAPRAAAGVDLRRAVAGQVPTLQEEFA
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MRHFPIFLDLRGRRVVLAGGGEAALAKLRLILKSEARVTVFATDPAPEIVAWDAERRLRL
CCCCCEEEEECCCEEEEECCCHHHHHHHHHHEECCCEEEEEECCCCCCEEEECHHHHHHH
VKRTFGPGDALCASLAYAASEDEAEDARVATLARADGALINIVDNLEGSAFITPAIVDRD
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCC
PVTIAIGTEGAAPVLARRIKADLEAQLPASLGLLARIGQGFRTAANALPMGRKRRDFWSD
CEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHH
FYFKKGPEALAKEGKQGVELALGDLLIDHLHCEETPGRVDLVGAGPGDPDLLTLKARKLL
HHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEHHHHHHH
DQADVVIYDRLVSPEILELARREATMVNVGKKGFGPSTKQDDIAAAMIAHASQGAHVVRL
HHCCEEEEECCCCHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
KSGDPGVYGRLDEEIDALDAAGIDWSIVPGITAANAASAQIGQSLTKRGRNGAMRFLTGH
ECCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECC
DVKGFAEQDWRSLAKPGQVAAIYMGAKGARFLQGRLMMHGIAPDTPVTAVENASRTDARI
CCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCEEECCCCCCCCHHHCCCCCCHHHH
IASTIATLPMDLDDAAPDGPVILMLGLAPRAAAGVDLRRAVAGQVPTLQEEFA
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA