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The map label for this gene is tpiA

Identifier: 89054224

GI number: 89054224

Start: 1726402

End: 1727148

Strand: Reverse

Name: tpiA

Synonym: Jann_1733

Alternate gene names: 89054224

Gene position: 1727148-1726402 (Counterclockwise)

Preceding gene: 89054226

Following gene: 89054222

Centisome position: 40.0

GC content: 66.53

Gene sequence:

>747_bases
ATGGCACGGAAACTGGCGGCAGGCAATTGGAAGATGAACGGGGTAGAGGCCGATTTGGCCGAGGTGGACGCTTTGGGCGA
GGCTGTTGCGGCGGCCAATTGCGACGTCTTGCTGTGCCCGCCCGCGACGCTGATTGCGCCGATGGCCAAGCGCGCGGGGT
TGATGGACCTCTATGTCGGCGGCCAGACATGCCATACGGCCGCGTCGGGCGCGCACACTGGCGACGTGTCGGCGGCGATG
CTGGCCGACGCGGGCGCGAGCCACGTGATCCTTGGCCATTCGGAACGACGCGCAGACCATGGCGAGCGCAGCGAAGATGT
GGCGGCCCAGGTCACGGCCGCCATTGACGCCAACCTGATCGCGATCATCTGCGTCGGCGAAACCGAAGCCGAGCGTGATG
CGAATGTGACCCTGAACGTCGTCTCCAGCCAGTTGGCAGGCTCCATCCCCACCGGCGCCACGCCCGCGCAGATCGTCGTG
GCCTATGAGCCCGTCTGGGCCATCGGCACGGGCCGCACGCCGACGCTGGAACAGATTGCCGAGGTCCATGATCACATCCG
GTCTGAACTGGCCGCGCGCCGGGGCAGCGCCGCCAATGATATCCCGCTGCTCTATGGCGGATCGGTCAAGCCCGGCAATG
CCGCAGAGATCTTTGCGGTGTCCAATGTGGACGGCGCGCTGGTCGGTGGCGCATCGTTGAAGGCGTCGGATTTCGGCGGA
ATTATCGCCGCCCTGTCCGCCGCCTGA

Upstream 100 bases:

>100_bases
GCGTTTGCGTCGATATGCGGGATCATCGGGGACCTCCTGTCGGGTTGACCGCCTGTGCCGTGGGTGCGACCACGGGAACA
AATGAACTCTGGAGGCGGCG

Downstream 100 bases:

>100_bases
CCGCCCGCACGTCAAGGAAAAGGCCGCCCACCGCGTGTGCGAGGGGCGGCCTTTTTGGTTTCAGGTCATGTCACGGCGCG
GGAACAAACCGCTCCGTCGC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MARKLAAGNWKMNGVEADLAEVDALGEAVAAANCDVLLCPPATLIAPMAKRAGLMDLYVGGQTCHTAASGAHTGDVSAAM
LADAGASHVILGHSERRADHGERSEDVAAQVTAAIDANLIAIICVGETEAERDANVTLNVVSSQLAGSIPTGATPAQIVV
AYEPVWAIGTGRTPTLEQIAEVHDHIRSELAARRGSAANDIPLLYGGSVKPGNAAEIFAVSNVDGALVGGASLKASDFGG
IIAALSAA

Sequences:

>Translated_248_residues
MARKLAAGNWKMNGVEADLAEVDALGEAVAAANCDVLLCPPATLIAPMAKRAGLMDLYVGGQTCHTAASGAHTGDVSAAM
LADAGASHVILGHSERRADHGERSEDVAAQVTAAIDANLIAIICVGETEAERDANVTLNVVSSQLAGSIPTGATPAQIVV
AYEPVWAIGTGRTPTLEQIAEVHDHIRSELAARRGSAANDIPLLYGGSVKPGNAAEIFAVSNVDGALVGGASLKASDFGG
IIAALSAA
>Mature_247_residues
ARKLAAGNWKMNGVEADLAEVDALGEAVAAANCDVLLCPPATLIAPMAKRAGLMDLYVGGQTCHTAASGAHTGDVSAAML
ADAGASHVILGHSERRADHGERSEDVAAQVTAAIDANLIAIICVGETEAERDANVTLNVVSSQLAGSIPTGATPAQIVVA
YEPVWAIGTGRTPTLEQIAEVHDHIRSELAARRGSAANDIPLLYGGSVKPGNAAEIFAVSNVDGALVGGASLKASDFGGI
IAALSAA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=40.2439024390244, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=40.2439024390244, Blast_Score=159, Evalue=2e-39,
Organism=Escherichia coli, GI1790353, Length=253, Percent_Identity=43.0830039525692, Blast_Score=177, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17536593, Length=252, Percent_Identity=43.2539682539683, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320255, Length=237, Percent_Identity=41.3502109704641, Blast_Score=163, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28572008, Length=250, Percent_Identity=42.8, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI28572006, Length=250, Percent_Identity=42.8, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI28572004, Length=250, Percent_Identity=42.8, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_JANSC (Q28RL2)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509675.1
- ProteinModelPortal:   Q28RL2
- SMR:   Q28RL2
- STRING:   Q28RL2
- GeneID:   3934181
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1733
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   IEKNGTM
- BioCyc:   JSP290400:JANN_1733-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 24979; Mature: 24848

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 93-93 ACT_SITE 163-163 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKLAAGNWKMNGVEADLAEVDALGEAVAAANCDVLLCPPATLIAPMAKRAGLMDLYVG
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCEEEEEEC
GQTCHTAASGAHTGDVSAAMLADAGASHVILGHSERRADHGERSEDVAAQVTAAIDANLI
CCHHHHCCCCCCCCCCHHHHHHCCCCCEEEEECCHHCCCCCCCCHHHHHHHHHHCCCCEE
AIICVGETEAERDANVTLNVVSSQLAGSIPTGATPAQIVVAYEPVWAIGTGRTPTLEQIA
EEEEECCCCCCCCCCEEEEEEHHHHCCCCCCCCCCEEEEEEECCEEEECCCCCCHHHHHH
EVHDHIRSELAARRGSAANDIPLLYGGSVKPGNAAEIFAVSNVDGALVGGASLKASDFGG
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCH
IIAALSAA
HHHHEECC
>Mature Secondary Structure 
ARKLAAGNWKMNGVEADLAEVDALGEAVAAANCDVLLCPPATLIAPMAKRAGLMDLYVG
CCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCEEEEEEC
GQTCHTAASGAHTGDVSAAMLADAGASHVILGHSERRADHGERSEDVAAQVTAAIDANLI
CCHHHHCCCCCCCCCCHHHHHHCCCCCEEEEECCHHCCCCCCCCHHHHHHHHHHCCCCEE
AIICVGETEAERDANVTLNVVSSQLAGSIPTGATPAQIVVAYEPVWAIGTGRTPTLEQIA
EEEEECCCCCCCCCCEEEEEEHHHHCCCCCCCCCCEEEEEEECCEEEECCCCCCHHHHHH
EVHDHIRSELAARRGSAANDIPLLYGGSVKPGNAAEIFAVSNVDGALVGGASLKASDFGG
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCH
IIAALSAA
HHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA