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The map label for this gene is proS

Identifier: 89053551

GI number: 89053551

Start: 1015865

End: 1017223

Strand: Direct

Name: proS

Synonym: Jann_1060

Alternate gene names: 89053551

Gene position: 1015865-1017223 (Clockwise)

Preceding gene: 89053550

Following gene: 89053555

Centisome position: 23.53

GC content: 61.88

Gene sequence:

>1359_bases
ATGCGCCTCAGCCGCTATTTCCTTCCCGTCCTGAAAGAAACGCCCGCCGAGGCGCAGATCGTCAGCCACCGCCTGATGTT
GCGCGCGGGCATGATCAAGCAAGCCAGCGCCGGGATCTATTCGTGGCTTCCGCTTGGCTACAAGGTCCTCAAGCGCATTG
AACGGATCGTCCACGAAGAACAGATCCGCGCCGGTCACATCCCGATGCTGATGCCCACGCTGCAATCGGCGGATCTGTGG
CAAAAGTCCGGGCGGTTCGATGCTTACGGCCCCGAAATGCTGCGCCTGAAGGACCGCCATGACCGCGACATGCTATACGG
TCCCACCAATGAGGAAATGATCACCGATATGGTGGGCACGTTCGTGACCTCCTACAAATCGCTGCCCCTGACGCTCTATC
ACATCCAGTGGAAGTTCCGCGATGAGGTGCGTCCTAGGTTCGGCGTCATGCGGGGCCGTGAGTTCCTGATGAAGGACGGC
TACAACTTTGACCTGACGAAAGAGGCGGCGCTGCACGCCTACAACCGCCACCTCGTGTCCTACCTGCGCACCTATGAACG
GATGGGCCTGCAAGCGATCCCGATGCGGGCGGACAGCGGGCCGATTGGCGGCGATTATACCCATGAATTCCTCGTCCTCG
CCGACACCGGCGAGTCGGAGGTCTTCTACGACGCAGAGATCACCGATCTGAAGTTCGGCGACCGCGAGATCGACTATGAT
GATGTCGCACAGTGCGACGCGGTGTTGCAGGAATTTACCTCACGCTATGCGCGGACCGATGAGACCCATGATGAGGCCGA
GTTTGCCGCGATCCCCGGGGATCGTCAGCGCAGCGCGCGGGGCATCGAGGTGGGGCAGATCTTCTATTTCGGCACTGAGT
ATTCCGAAAAGTTGGGGGCCCATGTCCAGAATGATGAAGGGGAGCGGGTGCCGCTCCATATGGGATCCCACGGGATCGGC
GTGTCGCGTCTGTTGGGTGCGATCATTGAGGCGAGCCATGACGATAAGGGGATCATTTGGCCCGAGGGCGTGACGCCGTT
CCATTGCGGGATCGTCAACCTCAAGCAAGGCGATGCAGAGGCTGATGCGGCCTGCGAAGCGCTGGAAACGGCGCTGGAAA
ACGCAGGCCTTGAGCCGCTTTACGATGACCGGAACGAGCGGGCGGGCGGCAAGTTCGCGACGATGGACCTGATCGGCCTG
CCGTGGCGGATCACGGTGGGTCCGCGGGGCCTCAAGAATGGCGTGGTGGAGCTGACCAGCCGGCGCACGGGCGAAAGCGA
AGAGCTGACGCCCGACGCCGCAGTGGCGCGCGTGGCGGAGGTTTACGCCGCCCACAAACCCGTTAACGCAGCGGTCTGA

Upstream 100 bases:

>100_bases
TGCTCCTACCGTCCAGACCATCGCCGTCTTGTGAGGCAAACCCCTTTGGCGTAGACCGTCGGGACCAGTTTTGTCCCCGT
TTGTTCCAAGGTCTCCTCCC

Downstream 100 bases:

>100_bases
CGGCCCCGCGTCAAGGTGCTGACAAAAGGCAGGGAATCCGGACTATGGCAGGGGAGGTTCCGAGGGTAGTGTCCTCTCAA
GTCAGAAAGGACCCCTTATG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MRLSRYFLPVLKETPAEAQIVSHRLMLRAGMIKQASAGIYSWLPLGYKVLKRIERIVHEEQIRAGHIPMLMPTLQSADLW
QKSGRFDAYGPEMLRLKDRHDRDMLYGPTNEEMITDMVGTFVTSYKSLPLTLYHIQWKFRDEVRPRFGVMRGREFLMKDG
YNFDLTKEAALHAYNRHLVSYLRTYERMGLQAIPMRADSGPIGGDYTHEFLVLADTGESEVFYDAEITDLKFGDREIDYD
DVAQCDAVLQEFTSRYARTDETHDEAEFAAIPGDRQRSARGIEVGQIFYFGTEYSEKLGAHVQNDEGERVPLHMGSHGIG
VSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDAEADAACEALETALENAGLEPLYDDRNERAGGKFATMDLIGL
PWRITVGPRGLKNGVVELTSRRTGESEELTPDAAVARVAEVYAAHKPVNAAV

Sequences:

>Translated_452_residues
MRLSRYFLPVLKETPAEAQIVSHRLMLRAGMIKQASAGIYSWLPLGYKVLKRIERIVHEEQIRAGHIPMLMPTLQSADLW
QKSGRFDAYGPEMLRLKDRHDRDMLYGPTNEEMITDMVGTFVTSYKSLPLTLYHIQWKFRDEVRPRFGVMRGREFLMKDG
YNFDLTKEAALHAYNRHLVSYLRTYERMGLQAIPMRADSGPIGGDYTHEFLVLADTGESEVFYDAEITDLKFGDREIDYD
DVAQCDAVLQEFTSRYARTDETHDEAEFAAIPGDRQRSARGIEVGQIFYFGTEYSEKLGAHVQNDEGERVPLHMGSHGIG
VSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDAEADAACEALETALENAGLEPLYDDRNERAGGKFATMDLIGL
PWRITVGPRGLKNGVVELTSRRTGESEELTPDAAVARVAEVYAAHKPVNAAV
>Mature_452_residues
MRLSRYFLPVLKETPAEAQIVSHRLMLRAGMIKQASAGIYSWLPLGYKVLKRIERIVHEEQIRAGHIPMLMPTLQSADLW
QKSGRFDAYGPEMLRLKDRHDRDMLYGPTNEEMITDMVGTFVTSYKSLPLTLYHIQWKFRDEVRPRFGVMRGREFLMKDG
YNFDLTKEAALHAYNRHLVSYLRTYERMGLQAIPMRADSGPIGGDYTHEFLVLADTGESEVFYDAEITDLKFGDREIDYD
DVAQCDAVLQEFTSRYARTDETHDEAEFAAIPGDRQRSARGIEVGQIFYFGTEYSEKLGAHVQNDEGERVPLHMGSHGIG
VSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDAEADAACEALETALENAGLEPLYDDRNERAGGKFATMDLIGL
PWRITVGPRGLKNGVVELTSRRTGESEELTPDAAVARVAEVYAAHKPVNAAV

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=429, Percent_Identity=33.7995337995338, Blast_Score=238, Evalue=8e-63,
Organism=Escherichia coli, GI1786392, Length=223, Percent_Identity=54.2600896860987, Blast_Score=266, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI115532348, Length=400, Percent_Identity=31.75, Blast_Score=187, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI193203271, Length=88, Percent_Identity=35.2272727272727, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=220, Percent_Identity=30.9090909090909, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24656200, Length=462, Percent_Identity=30.7359307359307, Blast_Score=208, Evalue=7e-54,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_JANSC (Q28TI5)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509002.1
- ProteinModelPortal:   Q28TI5
- SMR:   Q28TI5
- STRING:   Q28TI5
- GeneID:   3933504
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1060
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q28TI5
- ProtClustDB:   PRK12325
- BioCyc:   JSP290400:JANN_1060-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 50845; Mature: 50845

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFLPVLKETPAEAQIVSHRLMLRAGMIKQASAGIYSWLPLGYKVLKRIERIVHEE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QIRAGHIPMLMPTLQSADLWQKSGRFDAYGPEMLRLKDRHDRDMLYGPTNEEMITDMVGT
HHHCCCCCEEHHCCCCHHHHHHCCCCCCCCCHHHEECCCCCCCCEECCCCHHHHHHHHHH
FVTSYKSLPLTLYHIQWKFRDEVRPRFGVMRGREFLMKDGYNFDLTKEAALHAYNRHLVS
HHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YLRTYERMGLQAIPMRADSGPIGGDYTHEFLVLADTGESEVFYDAEITDLKFGDREIDYD
HHHHHHHCCCEEECEECCCCCCCCCCCEEEEEEEECCCCCEEEECEEEEEECCCCCCCHH
DVAQCDAVLQEFTSRYARTDETHDEAEFAAIPGDRQRSARGIEVGQIFYFGTEYSEKLGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHCCCCCEEEEEEEECCCHHHHHCC
HVQNDEGERVPLHMGSHGIGVSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDAE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCH
ADAACEALETALENAGLEPLYDDRNERAGGKFATMDLIGLPWRITVGPRGLKNGVVELTS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCHHCCHHHHHH
RRTGESEELTPDAAVARVAEVYAAHKPVNAAV
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLSRYFLPVLKETPAEAQIVSHRLMLRAGMIKQASAGIYSWLPLGYKVLKRIERIVHEE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QIRAGHIPMLMPTLQSADLWQKSGRFDAYGPEMLRLKDRHDRDMLYGPTNEEMITDMVGT
HHHCCCCCEEHHCCCCHHHHHHCCCCCCCCCHHHEECCCCCCCCEECCCCHHHHHHHHHH
FVTSYKSLPLTLYHIQWKFRDEVRPRFGVMRGREFLMKDGYNFDLTKEAALHAYNRHLVS
HHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YLRTYERMGLQAIPMRADSGPIGGDYTHEFLVLADTGESEVFYDAEITDLKFGDREIDYD
HHHHHHHCCCEEECEECCCCCCCCCCCEEEEEEEECCCCCEEEECEEEEEECCCCCCCHH
DVAQCDAVLQEFTSRYARTDETHDEAEFAAIPGDRQRSARGIEVGQIFYFGTEYSEKLGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHCCCCCEEEEEEEECCCHHHHHCC
HVQNDEGERVPLHMGSHGIGVSRLLGAIIEASHDDKGIIWPEGVTPFHCGIVNLKQGDAE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCH
ADAACEALETALENAGLEPLYDDRNERAGGKFATMDLIGLPWRITVGPRGLKNGVVELTS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCHHCCHHHHHH
RRTGESEELTPDAAVARVAEVYAAHKPVNAAV
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA