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The map label for this gene is yjbJ [H]

Identifier: 89053493

GI number: 89053493

Start: 959842

End: 960657

Strand: Direct

Name: yjbJ [H]

Synonym: Jann_1002

Alternate gene names: 89053493

Gene position: 959842-960657 (Clockwise)

Preceding gene: 89053478

Following gene: 89053503

Centisome position: 22.23

GC content: 66.42

Gene sequence:

>816_bases
ATGGCCAGACGGATTTGGATAGCGACCGTGCTTGCGGTCGGGATGGCATGGCAGGTTGCCGCCCAGGACAGTGGGATACG
GTTCGTTCGCGCGCCTGAAGCGGGTGATACGGGTCCGCGTATCACGATTCAGATCACGGAAGAGGATATGGCGCGCCAGG
GTGCCCCGGCGGAGGCGCCGGCAACTGCACTCGGTCCCGTCGCGCCACCGTCAGATATGGCCTATGACGGGGCGTCGGCC
TGGTTCTGGGCAGAAGTGCCAAGCCAGATGCCCGCAGATCCCACCCGCTTCTGGGCCGCGCAGGAACATCTGGCGCTTGC
GCCTGAGGCCAGCGGTTTGGGCGCGCCGCGTCTGGATACCGTCAGCCGGATCGCCGCAACCCACGGGCGAGAGATTCTGG
CGGCCACCATCGGCACCCAGGTCTCTCCTGCATTCGCGTTGGCGGTGATTGCGGCGGAAAGCGCGGGCCGCCCGGAGGCG
GTCAGCGGGGCAGGCGCGCAGGGGTTGATGCAATTGATCCCTGCCACCGCAGAACGGTTCGGGGTGGAAGATTCCATGGA
CCCCTCCCAAAACATTGCAGGTGGCGTCGCCTATCTCGACTGGCTGATGGGAGAGTTCGACCGCGATCCGATTCTGGTCC
TGGCCGCGTATAATGCGGGGGAGGGGGCGGTGACGCGGGCGGGTGGCGTGCCTGACTATGACGAGACGCGCACCTATGTG
CCGAGGGTGCTGGCCGCGTGGGGCCTGGCGCGCGGCCTATGCAACACGCCCCCGGATCTGGTATCGGACGGATGCGTGTT
CCAGGTGATGAACTGA

Upstream 100 bases:

>100_bases
TTTGTGTCAGCCGCGCAGGCATGATTCCGATCAAAGAGCCGTGTTATGTGGGCGGAGACCTACAAACCCGCGCAATTTGA
GCAGTAAACACAGGTGCTTC

Downstream 100 bases:

>100_bases
CGCCAGCCCAAGGCGCTTTTTGAACAAATTCCTAGGAAAAGACGTCGGCCCATTCCGGGTGACGGCTTGCTTGCGCGCGG
ACGAAGGGGCAAAGGGCTAT

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MARRIWIATVLAVGMAWQVAAQDSGIRFVRAPEAGDTGPRITIQITEEDMARQGAPAEAPATALGPVAPPSDMAYDGASA
WFWAEVPSQMPADPTRFWAAQEHLALAPEASGLGAPRLDTVSRIAATHGREILAATIGTQVSPAFALAVIAAESAGRPEA
VSGAGAQGLMQLIPATAERFGVEDSMDPSQNIAGGVAYLDWLMGEFDRDPILVLAAYNAGEGAVTRAGGVPDYDETRTYV
PRVLAAWGLARGLCNTPPDLVSDGCVFQVMN

Sequences:

>Translated_271_residues
MARRIWIATVLAVGMAWQVAAQDSGIRFVRAPEAGDTGPRITIQITEEDMARQGAPAEAPATALGPVAPPSDMAYDGASA
WFWAEVPSQMPADPTRFWAAQEHLALAPEASGLGAPRLDTVSRIAATHGREILAATIGTQVSPAFALAVIAAESAGRPEA
VSGAGAQGLMQLIPATAERFGVEDSMDPSQNIAGGVAYLDWLMGEFDRDPILVLAAYNAGEGAVTRAGGVPDYDETRTYV
PRVLAAWGLARGLCNTPPDLVSDGCVFQVMN
>Mature_270_residues
ARRIWIATVLAVGMAWQVAAQDSGIRFVRAPEAGDTGPRITIQITEEDMARQGAPAEAPATALGPVAPPSDMAYDGASAW
FWAEVPSQMPADPTRFWAAQEHLALAPEASGLGAPRLDTVSRIAATHGREILAATIGTQVSPAFALAVIAAESAGRPEAV
SGAGAQGLMQLIPATAERFGVEDSMDPSQNIAGGVAYLDWLMGEFDRDPILVLAAYNAGEGAVTRAGGVPDYDETRTYVP
RVLAAWGLARGLCNTPPDLVSDGCVFQVMN

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=150, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 28328; Mature: 28197

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRIWIATVLAVGMAWQVAAQDSGIRFVRAPEAGDTGPRITIQITEEDMARQGAPAEAP
CCCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCEEEEEECHHHHHHCCCCCCCC
ATALGPVAPPSDMAYDGASAWFWAEVPSQMPADPTRFWAAQEHLALAPEASGLGAPRLDT
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCHHH
VSRIAATHGREILAATIGTQVSPAFALAVIAAESAGRPEAVSGAGAQGLMQLIPATAERF
HHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GVEDSMDPSQNIAGGVAYLDWLMGEFDRDPILVLAAYNAGEGAVTRAGGVPDYDETRTYV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCCCCHHHHHHH
PRVLAAWGLARGLCNTPPDLVSDGCVFQVMN
HHHHHHHHHHHHCCCCCHHHHCCCEEEEECC
>Mature Secondary Structure 
ARRIWIATVLAVGMAWQVAAQDSGIRFVRAPEAGDTGPRITIQITEEDMARQGAPAEAP
CCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCEEEEEECHHHHHHCCCCCCCC
ATALGPVAPPSDMAYDGASAWFWAEVPSQMPADPTRFWAAQEHLALAPEASGLGAPRLDT
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCHHH
VSRIAATHGREILAATIGTQVSPAFALAVIAAESAGRPEAVSGAGAQGLMQLIPATAERF
HHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GVEDSMDPSQNIAGGVAYLDWLMGEFDRDPILVLAAYNAGEGAVTRAGGVPDYDETRTYV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCCCCHHHHHHH
PRVLAAWGLARGLCNTPPDLVSDGCVFQVMN
HHHHHHHHHHHHCCCCCHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]