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The map label for this gene is folD

Identifier: 89053474

GI number: 89053474

Start: 939684

End: 940610

Strand: Direct

Name: folD

Synonym: Jann_0983

Alternate gene names: 89053474

Gene position: 939684-940610 (Clockwise)

Preceding gene: 89053473

Following gene: 89053478

Centisome position: 21.76

GC content: 63.86

Gene sequence:

>927_bases
ATGACGGAGCCCCAGACAAACCGCACCTCTGGTGCGAAGATCATCGACGGAAAAGCCTTCGCAGCCGATGTGCGCGGTCA
GGTGGCAGGCCATGTGCAGCGCTTGAAGGACGAGCATGGGATCACGCCCGGTTTGGCGGTCGTGCTGGTGGGTGAGGATC
CGGCGTCTGAGGTCTATGTCAGCCACAAACACAAGGCGACGGTGGAGGTCGGAATGACCTCCTTCGAGCACAAACTGCCT
GCCGACACCTCCGAGGACGACCTCTTTGCGCTGATCGACAAGTTGAACGCTGATCCAGCGGTCCACGGCATCCTTTGTCA
GTTCCCGGTGCCCGATCATCTGGACGAGCGGCGCACGGTTGCGCGGATCAGCCCGGCCAAGGACGTCGACGGGTTGAGCG
TGACCAATGCGGGGCTGTTGTCCAGCGGGGGCGAGGCCTTGGTGAGCTGTACGCCCCTGGGTTGCCTGATGCTGCTGCGG
GCCGAATTCGACAGCCTGTCCGGCAAGGACGCCGTTGTCATCGGACGGTCCAACCTATTCGGCAAGCCCATGGCGCAGTT
GCTGCTCGGCGACAATTGCACGGTCACGATTGCGCATTCCCGGACGAAGGACCTGGCCGACGTCTGCCGCCGGGCCGATA
TCCTCGTGGCCGCCGTTGGCCGGGCCGAGATGGTGCGGGGCGATTGGATCAAGCCGGGTGCGACTGTCATCGACGTGGGC
ATCTCTCGCGTGCCGCATCCCGACAAGCCGGGCAAAACCAAGCTGATCGGTGACGTGCATTTCGCGGAAGCGGTTGATGT
TGCGGGCGCGATTACGCCTGTGCCGGGCGGTGTGGGGCCGATGACCATTGCCTGCCTTCTGGCAAATACGGTCACCGCAT
GTTGCCGCGCCCACGGTTTGGACGAACCTCGGGGGCTGACCGCTTAG

Upstream 100 bases:

>100_bases
CCGAGACCCATCAAGCGCTGCACACGCGCGCGCATCACGCCTGTTTCATCGCGAATTCTGTCCGATCAGACATCGTGGTC
AACCTATTGGAGGACGCCCC

Downstream 100 bases:

>100_bases
TCCGTTTCAGGCCCGTCAAAGCGGGATCATCGTTCCCTGAAGCAGCGCCACATCGCGGAGGCTGCCATCCTCGGCCTCCG
CCTGCACAGTGCAGCGGGTG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTEPQTNRTSGAKIIDGKAFAADVRGQVAGHVQRLKDEHGITPGLAVVLVGEDPASEVYVSHKHKATVEVGMTSFEHKLP
ADTSEDDLFALIDKLNADPAVHGILCQFPVPDHLDERRTVARISPAKDVDGLSVTNAGLLSSGGEALVSCTPLGCLMLLR
AEFDSLSGKDAVVIGRSNLFGKPMAQLLLGDNCTVTIAHSRTKDLADVCRRADILVAAVGRAEMVRGDWIKPGATVIDVG
ISRVPHPDKPGKTKLIGDVHFAEAVDVAGAITPVPGGVGPMTIACLLANTVTACCRAHGLDEPRGLTA

Sequences:

>Translated_308_residues
MTEPQTNRTSGAKIIDGKAFAADVRGQVAGHVQRLKDEHGITPGLAVVLVGEDPASEVYVSHKHKATVEVGMTSFEHKLP
ADTSEDDLFALIDKLNADPAVHGILCQFPVPDHLDERRTVARISPAKDVDGLSVTNAGLLSSGGEALVSCTPLGCLMLLR
AEFDSLSGKDAVVIGRSNLFGKPMAQLLLGDNCTVTIAHSRTKDLADVCRRADILVAAVGRAEMVRGDWIKPGATVIDVG
ISRVPHPDKPGKTKLIGDVHFAEAVDVAGAITPVPGGVGPMTIACLLANTVTACCRAHGLDEPRGLTA
>Mature_307_residues
TEPQTNRTSGAKIIDGKAFAADVRGQVAGHVQRLKDEHGITPGLAVVLVGEDPASEVYVSHKHKATVEVGMTSFEHKLPA
DTSEDDLFALIDKLNADPAVHGILCQFPVPDHLDERRTVARISPAKDVDGLSVTNAGLLSSGGEALVSCTPLGCLMLLRA
EFDSLSGKDAVVIGRSNLFGKPMAQLLLGDNCTVTIAHSRTKDLADVCRRADILVAAVGRAEMVRGDWIKPGATVIDVGI
SRVPHPDKPGKTKLIGDVHFAEAVDVAGAITPVPGGVGPMTIACLLANTVTACCRAHGLDEPRGLTA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=44.1379310344828, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI222418558, Length=303, Percent_Identity=40.5940594059406, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI94721354, Length=301, Percent_Identity=41.5282392026578, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI36796743, Length=200, Percent_Identity=27.5, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786741, Length=288, Percent_Identity=48.9583333333333, Blast_Score=247, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=41.296928327645, Blast_Score=200, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6319558, Length=294, Percent_Identity=42.1768707482993, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=295, Percent_Identity=42.0338983050847, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=306, Percent_Identity=26.1437908496732, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI62472483, Length=293, Percent_Identity=46.0750853242321, Blast_Score=247, Evalue=9e-66,
Organism=Drosophila melanogaster, GI45551871, Length=293, Percent_Identity=46.0750853242321, Blast_Score=247, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=46.3917525773196, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=46.3917525773196, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=47.1186440677966, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=47.6027397260274, Blast_Score=237, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_JANSC (Q28TR2)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508925.1
- ProteinModelPortal:   Q28TR2
- SMR:   Q28TR2
- STRING:   Q28TR2
- GeneID:   3933427
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0983
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- PhylomeDB:   Q28TR2
- ProtClustDB:   PRK14188
- BioCyc:   JSP290400:JANN_0983-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32248; Mature: 32117

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPQTNRTSGAKIIDGKAFAADVRGQVAGHVQRLKDEHGITPGLAVVLVGEDPASEVYV
CCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEE
SHKHKATVEVGMTSFEHKLPADTSEDDLFALIDKLNADPAVHGILCQFPVPDHLDERRTV
ECCCCEEEEECCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHH
ARISPAKDVDGLSVTNAGLLSSGGEALVSCTPLGCLMLLRAEFDSLSGKDAVVIGRSNLF
HCCCCCCCCCCCEECCCCHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCC
GKPMAQLLLGDNCTVTIAHSRTKDLADVCRRADILVAAVGRAEMVRGDWIKPGATVIDVG
CCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCEEEEECCCHHHHCCCCCCCCCEEEEEC
ISRVPHPDKPGKTKLIGDVHFAEAVDVAGAITPVPGGVGPMTIACLLANTVTACCRAHGL
HHCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
DEPRGLTA
CCCCCCCC
>Mature Secondary Structure 
TEPQTNRTSGAKIIDGKAFAADVRGQVAGHVQRLKDEHGITPGLAVVLVGEDPASEVYV
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEE
SHKHKATVEVGMTSFEHKLPADTSEDDLFALIDKLNADPAVHGILCQFPVPDHLDERRTV
ECCCCEEEEECCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHH
ARISPAKDVDGLSVTNAGLLSSGGEALVSCTPLGCLMLLRAEFDSLSGKDAVVIGRSNLF
HCCCCCCCCCCCEECCCCHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCC
GKPMAQLLLGDNCTVTIAHSRTKDLADVCRRADILVAAVGRAEMVRGDWIKPGATVIDVG
CCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCEEEEECCCHHHHCCCCCCCCCEEEEEC
ISRVPHPDKPGKTKLIGDVHFAEAVDVAGAITPVPGGVGPMTIACLLANTVTACCRAHGL
HHCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
DEPRGLTA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA