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The map label for this gene is mdh

Identifier: 89053309

GI number: 89053309

Start: 775544

End: 776506

Strand: Direct

Name: mdh

Synonym: Jann_0818

Alternate gene names: 89053309

Gene position: 775544-776506 (Clockwise)

Preceding gene: 89053308

Following gene: 89053310

Centisome position: 17.96

GC content: 61.99

Gene sequence:

>963_bases
ATGGCCAGACCCAAGATTGCGCTGATTGGCGCGGGACAGATCGGTGGCACCCTTGCCCACCTTGCGGCGTTGAAGGAATT
GGGGGACGTGGTCCTCTTTGACATCGCGGAAGGCGTGCCCCAGGGCAAAGCGCTAGACATCGCGGAATCAGGCCCGTCCG
AGAAATTTGACGCCGACATGAGCGGCACCAACGACTACGCCGACATCGCAGGCGCGGACGTTTGCATCGTCACCGCCGGG
GTCGCCCGCAAGCCGGGTATGAGCCGCGATGACCTGCTGGGGATCAACCTCAAGGTGATGAAATCGGTCGGCGAAGGCAT
CCGCGATAACGCGCCGGATGCGTTTGTGATCTGCATCACCAACCCGCTGGACGCCATGGTTTGGGCGCTGCGCGAATTCT
CTGGCCTGCCGCACCATAAGGTCTGCGGCATGGCGGGTGTTCTGGATTCTGCGCGCTTCCGCCATTTCCTGGCAGATGAA
TTCAACGTTTCCATGAAAGACGTCACGGCCTTCGTCTTGGGCGGTCACGGTGACACGATGGTGCCGCTGACCCGCTATTC
CACCGTGGCCGGTATTCCGCTGCCGGATCTGATCGAGATGGGGTGGACCACGCAGGAGAAGATGGATGCCATCGTCCAGC
GCACCCGGGATGGCGGCGCTGAGATCGTGGGCCTGCTGAAGACCGGATCGGCCTTCTACGCCCCCGCGACGTCCGCGATT
GAGATGGCCGAAGCCTACCTCAAGGACCAGAAGCGCGTGCTGCCCTGTGCGGCCTATGTGGACGGTGCGCTTGGCCTCAA
GGGCATGTATGTGGGCGTGCCCACCGTGATCGGCGCCGGTGGCGTTGAGCGTGTGGTCGACATCAATATGACCAAGGACG
AGCAGGCGATGTTCGATAATTCCGTCGCTGCGGTGAACGGTCTGGTTGAGGCTTGCAAGGCCATCGACGAAACGCTGTCC
TAA

Upstream 100 bases:

>100_bases
GGCGTTGGGGCTATCGGCCGCGCATCAGGTGACTTGCACCTGTGGCGTGCGGCGGCTACCCCTCCGCGCGACATAACTCA
AAATCCGAGGACATTGAGAC

Downstream 100 bases:

>100_bases
GCGTCGTTTGAGAGTGTGAAGGGGGTCGGGTGTTCGCATCCGGCCCCTTTTTTGTGCGCGGCGCGGGGCGAGATAATCCT
TGCGGGGGCGGGCGCAAGTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGVPQGKALDIAESGPSEKFDADMSGTNDYADIAGADVCIVTAG
VARKPGMSRDDLLGINLKVMKSVGEGIRDNAPDAFVICITNPLDAMVWALREFSGLPHHKVCGMAGVLDSARFRHFLADE
FNVSMKDVTAFVLGGHGDTMVPLTRYSTVAGIPLPDLIEMGWTTQEKMDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAI
EMAEAYLKDQKRVLPCAAYVDGALGLKGMYVGVPTVIGAGGVERVVDINMTKDEQAMFDNSVAAVNGLVEACKAIDETLS

Sequences:

>Translated_320_residues
MARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGVPQGKALDIAESGPSEKFDADMSGTNDYADIAGADVCIVTAG
VARKPGMSRDDLLGINLKVMKSVGEGIRDNAPDAFVICITNPLDAMVWALREFSGLPHHKVCGMAGVLDSARFRHFLADE
FNVSMKDVTAFVLGGHGDTMVPLTRYSTVAGIPLPDLIEMGWTTQEKMDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAI
EMAEAYLKDQKRVLPCAAYVDGALGLKGMYVGVPTVIGAGGVERVVDINMTKDEQAMFDNSVAAVNGLVEACKAIDETLS
>Mature_319_residues
ARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGVPQGKALDIAESGPSEKFDADMSGTNDYADIAGADVCIVTAGV
ARKPGMSRDDLLGINLKVMKSVGEGIRDNAPDAFVICITNPLDAMVWALREFSGLPHHKVCGMAGVLDSARFRHFLADEF
NVSMKDVTAFVLGGHGDTMVPLTRYSTVAGIPLPDLIEMGWTTQEKMDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAIE
MAEAYLKDQKRVLPCAAYVDGALGLKGMYVGVPTVIGAGGVERVVDINMTKDEQAMFDNSVAAVNGLVEACKAIDETLS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=301, Percent_Identity=29.2358803986711, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI221136809, Length=301, Percent_Identity=29.2358803986711, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI9257228, Length=304, Percent_Identity=30.9210526315789, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4504973, Length=304, Percent_Identity=30.9210526315789, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI291575128, Length=304, Percent_Identity=29.9342105263158, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI4557032, Length=304, Percent_Identity=29.9342105263158, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI260099723, Length=308, Percent_Identity=27.5974025974026, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI5031857, Length=308, Percent_Identity=27.5974025974026, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI15082234, Length=301, Percent_Identity=26.2458471760797, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI260099727, Length=198, Percent_Identity=30.8080808080808, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI260099725, Length=198, Percent_Identity=30.8080808080808, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=27.6923076923077, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI21735621, Length=329, Percent_Identity=25.531914893617, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789632, Length=293, Percent_Identity=32.0819112627986, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=28.0821917808219, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17554310, Length=321, Percent_Identity=30.8411214953271, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=306, Percent_Identity=26.797385620915, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320125, Length=327, Percent_Identity=25.0764525993884, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324446, Length=253, Percent_Identity=26.8774703557312, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17136226, Length=301, Percent_Identity=28.9036544850498, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45550422, Length=318, Percent_Identity=26.7295597484277, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24647881, Length=291, Percent_Identity=29.8969072164948, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24663595, Length=276, Percent_Identity=27.1739130434783, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24663599, Length=256, Percent_Identity=27.734375, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_JANSC (Q28U77)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508760.1
- ProteinModelPortal:   Q28U77
- SMR:   Q28U77
- STRING:   Q28U77
- GeneID:   3933261
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0818
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- PhylomeDB:   Q28U77
- ProtClustDB:   PRK06223
- BioCyc:   JSP290400:JANN_0818-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33647; Mature: 33516

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGVPQGKALDIAESGPSEKFDADM
CCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCEEECCCCCCCCCCCCCC
SGTNDYADIAGADVCIVTAGVARKPGMSRDDLLGINLKVMKSVGEGIRDNAPDAFVICIT
CCCCCHHHHCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCCCCCCCCEEEEEEC
NPLDAMVWALREFSGLPHHKVCGMAGVLDSARFRHFLADEFNVSMKDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCE
VPLTRYSTVAGIPLPDLIEMGWTTQEKMDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAI
EEEHHHCEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCHHHH
EMAEAYLKDQKRVLPCAAYVDGALGLKGMYVGVPTVIGAGGVERVVDINMTKDEQAMFDN
HHHHHHHHHHHHHCHHHHHHHHCCCCCEEEECCCHHHCCCCCCEEEEECCCCCHHHHHCC
SVAAVNGLVEACKAIDETLS
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGVPQGKALDIAESGPSEKFDADM
CCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCEEECCCCCCCCCCCCCC
SGTNDYADIAGADVCIVTAGVARKPGMSRDDLLGINLKVMKSVGEGIRDNAPDAFVICIT
CCCCCHHHHCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCCCCCCCCEEEEEEC
NPLDAMVWALREFSGLPHHKVCGMAGVLDSARFRHFLADEFNVSMKDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCE
VPLTRYSTVAGIPLPDLIEMGWTTQEKMDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAI
EEEHHHCEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCHHHH
EMAEAYLKDQKRVLPCAAYVDGALGLKGMYVGVPTVIGAGGVERVVDINMTKDEQAMFDN
HHHHHHHHHHHHHCHHHHHHHHCCCCCEEEECCCHHHCCCCCCEEEEECCCCCHHHHHCC
SVAAVNGLVEACKAIDETLS
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA