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The map label for this gene is thiC

Identifier: 88658541

GI number: 88658541

Start: 813891

End: 815555

Strand: Direct

Name: thiC

Synonym: ECH_0798

Alternate gene names: 88658541

Gene position: 813891-815555 (Clockwise)

Preceding gene: 88657922

Following gene: 88658068

Centisome position: 69.19

GC content: 33.99

Gene sequence:

>1665_bases
ATGAAAATAGACTTTAATACCCTCTTTCCTTCTTCAACTAAAGAATACATATCAGGCACAATATACAACAATATAAAAGT
TGGAATGCGTAGAATTCATATTAATGATAACAGTGAATCCATTCTAACATATGATACTGGAGGACCACATACTGATCAAA
AAATCAAAATTGACATTAATCAGGGTATTGAAAAGATAAGGCTTAATTGGATTGTAGATAGACAAGACGTAGAGTATCAT
AAAAGACAAGAAGTAAACACAAATTCTGAATACGCATTTCCACTACAAAGTAATAATATCTTAAAAGCAAATAGTAATAA
ACCTATAACCCAGATGTATTATGCTCGAAACAATATTATTACTCCAGAAATGGAATATGTAGCAATACGAGAAAACGCAC
TAAGACAAAAGATTCTTTCTTATAAACCAACTGTCATGGCACCAGAAATTACTCCAGAATTCGTACGACAAGAAATAGCA
TCTGGGAGAGCAATCATTCCAGCAAATGTAAATCATCCTGAATCAGAACCAATGATAATAGGCAAGAATTTCTTAGTAAA
AATCAATGCTAATATTGGTAATTCAGTAGTTAGTTCAAGCATTGAAGACGAACTTCAAAAAATGATATACGCAATTATAT
ACGGTGCTGATACAGTTATGGATTTATCTACAGGGAACAATATACATAATATTAGAGAATGGATTATCAGAAACAGCCCA
GTACCAATAGGTACAGTACCTATATATCAAGCATTAAACAAAGTAAATGGAGTAGTAGGAGACCTAGATTTCAATATCTT
CAAAAAAACATTAATAGAACAAGCAGAACAAGGTGTTGACTATTTTACCATACATGCAGGAGTATTAAAAAATTACATTG
ACTACACAGATAACAGGCTAACTGGCATCGTATCAAGAGGTGGAGCTATTATGGCACACTGGTGTACTATACATAATAAA
GAGAACTTTCTTTATACAAATTTTGAAGAAATATGCGATATTATGAAACACTATGACATTACTTTCTCTCTAGGAGATGG
ATTAAGGCCAGGATCCATAGCAGATGCAAACGATACAGCACAATTTTTAGAACTCAAAACACTAGGAGAACTTACAGACA
TTGCATGGAAGCACGATTGCCAAGTTATGATAGAAGGACCAGGCCATGTTCCTATGCATTTAATAAAAGAAAATGTAGAA
AAACAGGTTCACTTCTGTAAGGAAGCTCCATTCTATACTTTAGGACCATTAACTACAGACATTGCTCCAGGTTACGACCA
CATAACGAGTGCAATCGGCGCAGCAATTATAGGATGGTATGGCACTTCTATGTTGTGTTATGTTACACCGAAAGAACACT
TAGGATTACCTAACATTCAAGACGTAAAAGATGGAGTCATTGCATATAAAATAGCAGCACATGCAGCAGACTTAGCAAAA
GGTAATCCATCTGCTTATATACGTGATTATGCATTAAGTTATGCAAGGTTCAATTTCAAATGGTATGATCAATTTAACTT
ATCTCTTGATCCAGAAACGGCAAAATCACTACACGATGAATCTCTTCCTTCTGAGAATGCAAAGTCTGCTCACTTCTGTT
CAATGTGTGGGCCAAAATTCTGCTCAATGAAATTAACTCATCAAATAAAATCTATTGAAGAGTAA

Upstream 100 bases:

>100_bases
TACAACTAATTAGATTGATTTAAAGTTTAAATTTTTACAAATATAGACATTATATTAAAACAAAATATACTATGTATTTA
TATAGTATCTTCTGGTCAAT

Downstream 100 bases:

>100_bases
CAAACCGCAACTATGTTATAAAGCTTGTTAAGACATATTTTCCAGATTCTGTGGATAATGGGGTTATTAATAAGAATAGT
TTAAGGCAGCATTTTTTTGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKIDFNTLFPSSTKEYISGTIYNNIKVGMRRIHINDNSESILTYDTGGPHTDQKIKIDINQGIEKIRLNWIVDRQDVEYH
KRQEVNTNSEYAFPLQSNNILKANSNKPITQMYYARNNIITPEMEYVAIRENALRQKILSYKPTVMAPEITPEFVRQEIA
SGRAIIPANVNHPESEPMIIGKNFLVKINANIGNSVVSSSIEDELQKMIYAIIYGADTVMDLSTGNNIHNIREWIIRNSP
VPIGTVPIYQALNKVNGVVGDLDFNIFKKTLIEQAEQGVDYFTIHAGVLKNYIDYTDNRLTGIVSRGGAIMAHWCTIHNK
ENFLYTNFEEICDIMKHYDITFSLGDGLRPGSIADANDTAQFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVE
KQVHFCKEAPFYTLGPLTTDIAPGYDHITSAIGAAIIGWYGTSMLCYVTPKEHLGLPNIQDVKDGVIAYKIAAHAADLAK
GNPSAYIRDYALSYARFNFKWYDQFNLSLDPETAKSLHDESLPSENAKSAHFCSMCGPKFCSMKLTHQIKSIEE

Sequences:

>Translated_554_residues
MKIDFNTLFPSSTKEYISGTIYNNIKVGMRRIHINDNSESILTYDTGGPHTDQKIKIDINQGIEKIRLNWIVDRQDVEYH
KRQEVNTNSEYAFPLQSNNILKANSNKPITQMYYARNNIITPEMEYVAIRENALRQKILSYKPTVMAPEITPEFVRQEIA
SGRAIIPANVNHPESEPMIIGKNFLVKINANIGNSVVSSSIEDELQKMIYAIIYGADTVMDLSTGNNIHNIREWIIRNSP
VPIGTVPIYQALNKVNGVVGDLDFNIFKKTLIEQAEQGVDYFTIHAGVLKNYIDYTDNRLTGIVSRGGAIMAHWCTIHNK
ENFLYTNFEEICDIMKHYDITFSLGDGLRPGSIADANDTAQFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVE
KQVHFCKEAPFYTLGPLTTDIAPGYDHITSAIGAAIIGWYGTSMLCYVTPKEHLGLPNIQDVKDGVIAYKIAAHAADLAK
GNPSAYIRDYALSYARFNFKWYDQFNLSLDPETAKSLHDESLPSENAKSAHFCSMCGPKFCSMKLTHQIKSIEE
>Mature_554_residues
MKIDFNTLFPSSTKEYISGTIYNNIKVGMRRIHINDNSESILTYDTGGPHTDQKIKIDINQGIEKIRLNWIVDRQDVEYH
KRQEVNTNSEYAFPLQSNNILKANSNKPITQMYYARNNIITPEMEYVAIRENALRQKILSYKPTVMAPEITPEFVRQEIA
SGRAIIPANVNHPESEPMIIGKNFLVKINANIGNSVVSSSIEDELQKMIYAIIYGADTVMDLSTGNNIHNIREWIIRNSP
VPIGTVPIYQALNKVNGVVGDLDFNIFKKTLIEQAEQGVDYFTIHAGVLKNYIDYTDNRLTGIVSRGGAIMAHWCTIHNK
ENFLYTNFEEICDIMKHYDITFSLGDGLRPGSIADANDTAQFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVE
KQVHFCKEAPFYTLGPLTTDIAPGYDHITSAIGAAIIGWYGTSMLCYVTPKEHLGLPNIQDVKDGVIAYKIAAHAADLAK
GNPSAYIRDYALSYARFNFKWYDQFNLSLDPETAKSLHDESLPSENAKSAHFCSMCGPKFCSMKLTHQIKSIEE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=576, Percent_Identity=60.2430555555556, Blast_Score=718, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_EHRCR (Q2GG37)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507596.1
- STRING:   Q2GG37
- GeneID:   3927286
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0798
- TIGR:   ECH_0798
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q2GG37
- ProtClustDB:   PRK09284
- BioCyc:   ECHA205920:ECH_0798-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 62441; Mature: 62441

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: BINDING 191-191 BINDING 220-220 BINDING 249-249 BINDING 285-285 BINDING 385-385 BINDING 412-412

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDFNTLFPSSTKEYISGTIYNNIKVGMRRIHINDNSESILTYDTGGPHTDQKIKIDIN
CEEECCCCCCCCCHHHHCCCEECCEEEEEEEEEECCCCCEEEEECCCCCCCCCEEEEEHH
QGIEKIRLNWIVDRQDVEYHKRQEVNTNSEYAFPLQSNNILKANSNKPITQMYYARNNII
CCCEEEEEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCHHHEEECCCCCC
TPEMEYVAIRENALRQKILSYKPTVMAPEITPEFVRQEIASGRAIIPANVNHPESEPMII
CCCHHEEEHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEE
GKNFLVKINANIGNSVVSSSIEDELQKMIYAIIYGADTVMDLSTGNNIHNIREWIIRNSP
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCC
VPIGTVPIYQALNKVNGVVGDLDFNIFKKTLIEQAEQGVDYFTIHAGVLKNYIDYTDNRL
CCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCE
TGIVSRGGAIMAHWCTIHNKENFLYTNFEEICDIMKHYDITFSLGDGLRPGSIADANDTA
EEEEECCCEEEEEEEEEECCCCEEEECHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHH
QFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVEKQVHFCKEAPFYTLGPLTTD
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
IAPGYDHITSAIGAAIIGWYGTSMLCYVTPKEHLGLPNIQDVKDGVIAYKIAAHAADLAK
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHHHC
GNPSAYIRDYALSYARFNFKWYDQFNLSLDPETAKSLHDESLPSENAKSAHFCSMCGPKF
CCCCHHHHHHHHHHHEEEEEEEEEECCEECCHHHHHHHHHCCCCCCCCCCHHHHHCCCHH
CSMKLTHQIKSIEE
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKIDFNTLFPSSTKEYISGTIYNNIKVGMRRIHINDNSESILTYDTGGPHTDQKIKIDIN
CEEECCCCCCCCCHHHHCCCEECCEEEEEEEEEECCCCCEEEEECCCCCCCCCEEEEEHH
QGIEKIRLNWIVDRQDVEYHKRQEVNTNSEYAFPLQSNNILKANSNKPITQMYYARNNII
CCCEEEEEEEEEECCCHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCHHHEEECCCCCC
TPEMEYVAIRENALRQKILSYKPTVMAPEITPEFVRQEIASGRAIIPANVNHPESEPMII
CCCHHEEEHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEE
GKNFLVKINANIGNSVVSSSIEDELQKMIYAIIYGADTVMDLSTGNNIHNIREWIIRNSP
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCC
VPIGTVPIYQALNKVNGVVGDLDFNIFKKTLIEQAEQGVDYFTIHAGVLKNYIDYTDNRL
CCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCE
TGIVSRGGAIMAHWCTIHNKENFLYTNFEEICDIMKHYDITFSLGDGLRPGSIADANDTA
EEEEECCCEEEEEEEEEECCCCEEEECHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHH
QFLELKTLGELTDIAWKHDCQVMIEGPGHVPMHLIKENVEKQVHFCKEAPFYTLGPLTTD
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
IAPGYDHITSAIGAAIIGWYGTSMLCYVTPKEHLGLPNIQDVKDGVIAYKIAAHAADLAK
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHHHC
GNPSAYIRDYALSYARFNFKWYDQFNLSLDPETAKSLHDESLPSENAKSAHFCSMCGPKF
CCCCHHHHHHHHHHHEEEEEEEEEECCEECCHHHHHHHHHCCCCCCCCCCHHHHHCCCHH
CSMKLTHQIKSIEE
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA