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The map label for this gene is rpoH [H]

Identifier: 88658518

GI number: 88658518

Start: 670231

End: 671124

Strand: Direct

Name: rpoH [H]

Synonym: ECH_0655

Alternate gene names: 88658518

Gene position: 670231-671124 (Clockwise)

Preceding gene: 88658336

Following gene: 88658477

Centisome position: 56.98

GC content: 31.43

Gene sequence:

>894_bases
ATGTTAACAAATTCTATATTTTCCCTAACTCAAGACAATTTAATGTCCTATATCAATGAAGTGCATGCATTTCCGATTTT
GTCTCCTGAAGAAGAAGACAGGTTAGCAAGAAATTGGTATGAAAATGGGATCGTTGCTGACGCACATAGGTTAGTTACTA
GTCATCTAAGGCTAGTAGTCAAAGTTGCATTAAGCTTTAAAAATTATGAATTGCCTCTTATAGAGCTAATAATGGAAGGA
AATATAGGGCTGATGCAGGCTGTAAAAAAGTTCAATCCCACTCTTGGCTTTAGGTTATCCACTTATGCTATTTGGTGGAT
CAAAGCTTTTATTAAGGACTATATTCTTAAATCTTGGTCGTGCATTAAAATTGGTACAACACAAGCACAAAGGAAGTTAT
TCTTTAGCTTAAGGAAAATTAAGAAAAAACTTTTTAAATATAACCACAATATTACAAAAGAAGATATAAAGCTAATTGCA
AATAAATGTTCAACTTCTGAACAAGAAGTAGAACAGATGAACAGGTATTTTCTCTACAGAGATAGATCCCTGAATGAACT
AGTATTCTCTAATGATAATCAAAATGGAGTCGAATTACAAGAGATTATAAAGTGTGATACCCCAAACCAAGAGGATACAT
ATTTACTAAATGAAGAGTTAAATATAAAAAAGGCTTTAATTTCACAAGCTTTATCAACACTAAATGAAAGATACCGCGAC
ATATTCATCAGGCGGCGACTCATCGAAGAACCAGATACTTTAGACAAATTAAGTCAAGAGTATAATATATCAAAAGAGAG
AGTTAGACAAATAGAAATGCATGCTTTTACTAAAGTAAAGAATTTTATTATATCTGAAAGAGAAAAACTAGGTCATTGTA
ATATCAATAGTTAA

Upstream 100 bases:

>100_bases
TTATATTAACAATTAATACACTAAAACCCTAAAAATTTACTATACATCATCATATCACTTTGATATAATACAGCGTTGTA
ATCAAGTTTCATATGTCAAT

Downstream 100 bases:

>100_bases
ATAAGAATAGCATATCGATCTAACTATACGCTTAACATAAAAAGAAACTAATATACATTAATCTTAGAAAAATAAAAGAA
GATGTTAAGAGAAATAAGAC

Product: RNA polymerase sigma-32 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLTNSIFSLTQDNLMSYINEVHAFPILSPEEEDRLARNWYENGIVADAHRLVTSHLRLVVKVALSFKNYELPLIELIMEG
NIGLMQAVKKFNPTLGFRLSTYAIWWIKAFIKDYILKSWSCIKIGTTQAQRKLFFSLRKIKKKLFKYNHNITKEDIKLIA
NKCSTSEQEVEQMNRYFLYRDRSLNELVFSNDNQNGVELQEIIKCDTPNQEDTYLLNEELNIKKALISQALSTLNERYRD
IFIRRRLIEEPDTLDKLSQEYNISKERVRQIEMHAFTKVKNFIISEREKLGHCNINS

Sequences:

>Translated_297_residues
MLTNSIFSLTQDNLMSYINEVHAFPILSPEEEDRLARNWYENGIVADAHRLVTSHLRLVVKVALSFKNYELPLIELIMEG
NIGLMQAVKKFNPTLGFRLSTYAIWWIKAFIKDYILKSWSCIKIGTTQAQRKLFFSLRKIKKKLFKYNHNITKEDIKLIA
NKCSTSEQEVEQMNRYFLYRDRSLNELVFSNDNQNGVELQEIIKCDTPNQEDTYLLNEELNIKKALISQALSTLNERYRD
IFIRRRLIEEPDTLDKLSQEYNISKERVRQIEMHAFTKVKNFIISEREKLGHCNINS
>Mature_297_residues
MLTNSIFSLTQDNLMSYINEVHAFPILSPEEEDRLARNWYENGIVADAHRLVTSHLRLVVKVALSFKNYELPLIELIMEG
NIGLMQAVKKFNPTLGFRLSTYAIWWIKAFIKDYILKSWSCIKIGTTQAQRKLFFSLRKIKKKLFKYNHNITKEDIKLIA
NKCSTSEQEVEQMNRYFLYRDRSLNELVFSNDNQNGVELQEIIKCDTPNQEDTYLLNEELNIKKALISQALSTLNERYRD
IFIRRRLIEEPDTLDKLSQEYNISKERVRQIEMHAFTKVKNFIISEREKLGHCNINS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=273, Percent_Identity=35.8974358974359, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1789098, Length=290, Percent_Identity=31.7241379310345, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789448, Length=253, Percent_Identity=26.4822134387352, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35053; Mature: 35053

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNSIFSLTQDNLMSYINEVHAFPILSPEEEDRLARNWYENGIVADAHRLVTSHLRLVV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KVALSFKNYELPLIELIMEGNIGLMQAVKKFNPTLGFRLSTYAIWWIKAFIKDYILKSWS
HHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
CIKIGTTQAQRKLFFSLRKIKKKLFKYNHNITKEDIKLIANKCSTSEQEVEQMNRYFLYR
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
DRSLNELVFSNDNQNGVELQEIIKCDTPNQEDTYLLNEELNIKKALISQALSTLNERYRD
CCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
IFIRRRLIEEPDTLDKLSQEYNISKERVRQIEMHAFTKVKNFIISEREKLGHCNINS
HHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLTNSIFSLTQDNLMSYINEVHAFPILSPEEEDRLARNWYENGIVADAHRLVTSHLRLVV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KVALSFKNYELPLIELIMEGNIGLMQAVKKFNPTLGFRLSTYAIWWIKAFIKDYILKSWS
HHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
CIKIGTTQAQRKLFFSLRKIKKKLFKYNHNITKEDIKLIANKCSTSEQEVEQMNRYFLYR
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
DRSLNELVFSNDNQNGVELQEIIKCDTPNQEDTYLLNEELNIKKALISQALSTLNERYRD
CCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
IFIRRRLIEEPDTLDKLSQEYNISKERVRQIEMHAFTKVKNFIISEREKLGHCNINS
HHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]