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The map label for this gene is fmt

Identifier: 88658426

GI number: 88658426

Start: 923020

End: 923931

Strand: Reverse

Name: fmt

Synonym: ECH_0897

Alternate gene names: 88658426

Gene position: 923931-923020 (Counterclockwise)

Preceding gene: 88657790

Following gene: 88657617

Centisome position: 78.55

GC content: 32.46

Gene sequence:

>912_bases
ATGAAAATAATATTTATGGGTTCTCCCGAATTTTCTGTACCGGCTCTATGTGCTCTTCTAGAAGAAAAAGATCACGAAGT
TATTGCTGTGTATACAAGGCTTCCTAAACCTGCTGGAAGGAGAGGAAAGGTGCTTACTAAAACACCTATACATATTATTG
CAGAGCAAAATAATATAGAGGTCAATACTCCAAAATCTTTAAAGCATGATTATGAGCAAGAAAAAATTTTTGCATTAAAT
CCAGATGTCATTGTAGTAGTTGCATATGGGTTAATTATTCCAGAGGCTGTGTTATCGATACCTAAGTACGGATGCATAAA
TATACATCCATCCTTGTTGCCAAGATGGCGTGGAGCAGCACCTATACATTATGCAATTCTGTCAGGTGACGAACAAACTG
GAGTTACCATTATGCAAATGAATGAGCTATGGGATGAAGGAGATATTCTGCTGCAAAGGGATATTCCTATAGATGAACAA
GATAACATTGATACTTTAAGCCAAAAACTATCTAACTTAGGAAGTTCAATGTTAATTGAAGTGCTTAATAATATTGATCA
TTTGGTTCCTATTAAACAAAATGCTAATGATGCTACATATACGAATAAAATAATAGATTTTCATATAGACTGTAATGAGG
AAGCTCAGGTTATATGTAGAAAAATTAGAGCTTTGTATCCTAAAGCTTTTTTATTTTTCAATGATAAAAGACTCAGAATA
TTAAAGGCTAATTATTGTCCTGATAGTGATTTGCAAAATTTAAAGCCTGGGACAGTGATTAATTCTCATATGCATATTAA
ATGTAAAAACGGTGTTTTAATTCCACTTATAGTGCAATTGGAAGGGAAAAATCCATGTAGCATTAATGATTTTATACATG
GTTATAGTATTTCAAATAGTTCTATTACTTGA

Upstream 100 bases:

>100_bases
ATAATTTTTTTTTGTATCAAGTGAAAGATTAGGTGGGACAAGCTTGCCTTATTTATATATTTAATATAAATAGGCTGTAT
ATAACTGAATGTATTTTGCT

Downstream 100 bases:

>100_bases
TTGGCTATTGATATGGGTTTGTATGTATTAATATATGATACGGTATGTTAAATATAATGTTTTATGTGTTTTCTTCTTAA
AAATATTAATTAAATAGTTG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKIIFMGSPEFSVPALCALLEEKDHEVIAVYTRLPKPAGRRGKVLTKTPIHIIAEQNNIEVNTPKSLKHDYEQEKIFALN
PDVIVVVAYGLIIPEAVLSIPKYGCINIHPSLLPRWRGAAPIHYAILSGDEQTGVTIMQMNELWDEGDILLQRDIPIDEQ
DNIDTLSQKLSNLGSSMLIEVLNNIDHLVPIKQNANDATYTNKIIDFHIDCNEEAQVICRKIRALYPKAFLFFNDKRLRI
LKANYCPDSDLQNLKPGTVINSHMHIKCKNGVLIPLIVQLEGKNPCSINDFIHGYSISNSSIT

Sequences:

>Translated_303_residues
MKIIFMGSPEFSVPALCALLEEKDHEVIAVYTRLPKPAGRRGKVLTKTPIHIIAEQNNIEVNTPKSLKHDYEQEKIFALN
PDVIVVVAYGLIIPEAVLSIPKYGCINIHPSLLPRWRGAAPIHYAILSGDEQTGVTIMQMNELWDEGDILLQRDIPIDEQ
DNIDTLSQKLSNLGSSMLIEVLNNIDHLVPIKQNANDATYTNKIIDFHIDCNEEAQVICRKIRALYPKAFLFFNDKRLRI
LKANYCPDSDLQNLKPGTVINSHMHIKCKNGVLIPLIVQLEGKNPCSINDFIHGYSISNSSIT
>Mature_303_residues
MKIIFMGSPEFSVPALCALLEEKDHEVIAVYTRLPKPAGRRGKVLTKTPIHIIAEQNNIEVNTPKSLKHDYEQEKIFALN
PDVIVVVAYGLIIPEAVLSIPKYGCINIHPSLLPRWRGAAPIHYAILSGDEQTGVTIMQMNELWDEGDILLQRDIPIDEQ
DNIDTLSQKLSNLGSSMLIEVLNNIDHLVPIKQNANDATYTNKIIDFHIDCNEEAQVICRKIRALYPKAFLFFNDKRLRI
LKANYCPDSDLQNLKPGTVINSHMHIKCKNGVLIPLIVQLEGKNPCSINDFIHGYSISNSSIT

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=321, Percent_Identity=31.4641744548287, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI21614513, Length=175, Percent_Identity=30.2857142857143, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI238814322, Length=236, Percent_Identity=27.5423728813559, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789683, Length=302, Percent_Identity=38.4105960264901, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1788589, Length=281, Percent_Identity=28.1138790035587, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI133930964, Length=181, Percent_Identity=26.5193370165746, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319458, Length=181, Percent_Identity=33.7016574585635, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45550868, Length=307, Percent_Identity=30.9446254071661, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571984, Length=227, Percent_Identity=34.8017621145374, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585660, Length=175, Percent_Identity=29.1428571428571, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_EHRCR (Q2GFU1)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507692.1
- ProteinModelPortal:   Q2GFU1
- SMR:   Q2GFU1
- STRING:   Q2GFU1
- GeneID:   3927834
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0897
- TIGR:   ECH_0897
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   WRGAGPI
- ProtClustDB:   CLSK749560
- BioCyc:   ECHA205920:ECH_0897-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34054; Mature: 34054

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIFMGSPEFSVPALCALLEEKDHEVIAVYTRLPKPAGRRGKVLTKTPIHIIAEQNNIE
CEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECCEEEEEECCCEE
VNTPKSLKHDYEQEKIFALNPDVIVVVAYGLIIPEAVLSIPKYGCINIHPSLLPRWRGAA
ECCCHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCC
PIHYAILSGDEQTGVTIMQMNELWDEGDILLQRDIPIDEQDNIDTLSQKLSNLGSSMLIE
CEEEEEECCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHH
VLNNIDHLVPIKQNANDATYTNKIIDFHIDCNEEAQVICRKIRALYPKAFLFFNDKRLRI
HHHCCHHEEEECCCCCCCEEECEEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCEEEE
LKANYCPDSDLQNLKPGTVINSHMHIKCKNGVLIPLIVQLEGKNPCSINDFIHGYSISNS
EEECCCCCCCHHHCCCCCEECCEEEEEECCCEEEEEEEEECCCCCCCHHHCEEEEECCCC
SIT
CCC
>Mature Secondary Structure
MKIIFMGSPEFSVPALCALLEEKDHEVIAVYTRLPKPAGRRGKVLTKTPIHIIAEQNNIE
CEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECCEEEEEECCCEE
VNTPKSLKHDYEQEKIFALNPDVIVVVAYGLIIPEAVLSIPKYGCINIHPSLLPRWRGAA
ECCCHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCC
PIHYAILSGDEQTGVTIMQMNELWDEGDILLQRDIPIDEQDNIDTLSQKLSNLGSSMLIE
CEEEEEECCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHH
VLNNIDHLVPIKQNANDATYTNKIIDFHIDCNEEAQVICRKIRALYPKAFLFFNDKRLRI
HHHCCHHEEEECCCCCCCEEECEEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCEEEE
LKANYCPDSDLQNLKPGTVINSHMHIKCKNGVLIPLIVQLEGKNPCSINDFIHGYSISNS
EEECCCCCCCHHHCCCCCEECCEEEEEECCCEEEEEEEEECCCCCCCHHHCEEEEECCCC
SIT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA