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The map label for this gene is ksgA [H]

Identifier: 88658336

GI number: 88658336

Start: 651140

End: 651973

Strand: Direct

Name: ksgA [H]

Synonym: ECH_0648

Alternate gene names: 88658336

Gene position: 651140-651973 (Clockwise)

Preceding gene: 88658203

Following gene: 88658518

Centisome position: 55.36

GC content: 26.74

Gene sequence:

>834_bases
ATGTATAATAAACATAAATTACCATCATATATTCTTATAATCATGAATCATATTAGTAAGATAAATCCTAAAAAAGAATT
AAGCCAATGTTTTATTTCGTCAACCCATATTACAGATCAAATAGTTAACTATGCTGGCAATATCAGTGATTACTCAATAA
TAGAAATTGGTCCAGGACTTGGTACAATGACTTATTCTATATTAAATAAAAACCCTAAAAAACTAATTTCTATAGAAAAA
GACCGTAGGTTATCAACAATACATGAAAAAATAGTTGAAGAATTTCAAGGAAAATACGAATTTATATTATCAGATGCACT
AAACATAGATTTACGTGATATTATTGAACCTCCAGTAAAAGTAATAGCAAACCTACCCTATCATATAGCAACTACTCTAT
TAATAAAATGGATGGATTATATTAATTTTTTTACTAGTTTTACATTAATGTTCCAAAAAGAAGTAGCAGATCGTATAGTA
GCACAACCAAATAATAAAAATTATGGTACACTATCTATACTCATTCAATTACTATCTAATGTGTATAAAATGGAGGATTT
TGGACCAGAAATATTTTCACCTCAACCCAAAGTAATGTCATCTGTAATTAACATTATTGCATTACCAGAACCCAGATTTC
ATGTAAATTATAGAAAGTTATCTCAAGTATTAAAAACAACATTTAGTGAACGTCGTAAAATGATACGCTCTACATTAAAA
AAACTTACCAATAATGCAGATGAAATGCTAGAATCTCTTAACATTGATAATAATTTACGTCCTGAAAACTTATCAATAGA
ACAATTCTGTCAAATTACAAATTGTATAAACTAA

Upstream 100 bases:

>100_bases
CCAGTTGAGCTAAGCCGGCTGGAACTTTTTGTAATTCTATATATAATATTATAAAAGTCAATACCCAGAATCATATTTTA
TATATTTAATATCACTTTAC

Downstream 100 bases:

>100_bases
ATATCCTATGTATGTGAGGCTTTCGATATAAATTACCAAAAATATATTGTTATTTTAAATAATAAACCTAGCTTTTACAA
TTCATCTAACAATTTTCTGG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MYNKHKLPSYILIIMNHISKINPKKELSQCFISSTHITDQIVNYAGNISDYSIIEIGPGLGTMTYSILNKNPKKLISIEK
DRRLSTIHEKIVEEFQGKYEFILSDALNIDLRDIIEPPVKVIANLPYHIATTLLIKWMDYINFFTSFTLMFQKEVADRIV
AQPNNKNYGTLSILIQLLSNVYKMEDFGPEIFSPQPKVMSSVINIIALPEPRFHVNYRKLSQVLKTTFSERRKMIRSTLK
KLTNNADEMLESLNIDNNLRPENLSIEQFCQITNCIN

Sequences:

>Translated_277_residues
MYNKHKLPSYILIIMNHISKINPKKELSQCFISSTHITDQIVNYAGNISDYSIIEIGPGLGTMTYSILNKNPKKLISIEK
DRRLSTIHEKIVEEFQGKYEFILSDALNIDLRDIIEPPVKVIANLPYHIATTLLIKWMDYINFFTSFTLMFQKEVADRIV
AQPNNKNYGTLSILIQLLSNVYKMEDFGPEIFSPQPKVMSSVINIIALPEPRFHVNYRKLSQVLKTTFSERRKMIRSTLK
KLTNNADEMLESLNIDNNLRPENLSIEQFCQITNCIN
>Mature_277_residues
MYNKHKLPSYILIIMNHISKINPKKELSQCFISSTHITDQIVNYAGNISDYSIIEIGPGLGTMTYSILNKNPKKLISIEK
DRRLSTIHEKIVEEFQGKYEFILSDALNIDLRDIIEPPVKVIANLPYHIATTLLIKWMDYINFFTSFTLMFQKEVADRIV
AQPNNKNYGTLSILIQLLSNVYKMEDFGPEIFSPQPKVMSSVINIIALPEPRFHVNYRKLSQVLKTTFSERRKMIRSTLK
KLTNNADEMLESLNIDNNLRPENLSIEQFCQITNCIN

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=275, Percent_Identity=31.6363636363636, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI7657198, Length=252, Percent_Identity=30.952380952381, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1786236, Length=258, Percent_Identity=33.7209302325581, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25141369, Length=291, Percent_Identity=26.4604810996564, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=217, Percent_Identity=28.110599078341, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=222, Percent_Identity=29.2792792792793, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357273, Length=277, Percent_Identity=28.8808664259928, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21358017, Length=234, Percent_Identity=32.4786324786325, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32034; Mature: 32034

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNKHKLPSYILIIMNHISKINPKKELSQCFISSTHITDQIVNYAGNISDYSIIEIGPGL
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
GTMTYSILNKNPKKLISIEKDRRLSTIHEKIVEEFQGKYEFILSDALNIDLRDIIEPPVK
HHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHH
VIANLPYHIATTLLIKWMDYINFFTSFTLMFQKEVADRIVAQPNNKNYGTLSILIQLLSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VYKMEDFGPEIFSPQPKVMSSVINIIALPEPRFHVNYRKLSQVLKTTFSERRKMIRSTLK
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
KLTNNADEMLESLNIDNNLRPENLSIEQFCQITNCIN
HHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MYNKHKLPSYILIIMNHISKINPKKELSQCFISSTHITDQIVNYAGNISDYSIIEIGPGL
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
GTMTYSILNKNPKKLISIEKDRRLSTIHEKIVEEFQGKYEFILSDALNIDLRDIIEPPVK
HHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHH
VIANLPYHIATTLLIKWMDYINFFTSFTLMFQKEVADRIVAQPNNKNYGTLSILIQLLSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VYKMEDFGPEIFSPQPKVMSSVINIIALPEPRFHVNYRKLSQVLKTTFSERRKMIRSTLK
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
KLTNNADEMLESLNIDNNLRPENLSIEQFCQITNCIN
HHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA