Click here to switch to the map view.

The map label for this gene is nuoB

Identifier: 88658003

GI number: 88658003

Start: 803819

End: 804358

Strand: Direct

Name: nuoB

Synonym: ECH_0787

Alternate gene names: 88658003

Gene position: 803819-804358 (Clockwise)

Preceding gene: 88658152

Following gene: 88658220

Centisome position: 68.34

GC content: 38.15

Gene sequence:

>540_bases
ATGATAAACAAGAATGATTCTGTATTAAATAATCAATTCTGGCAAAGTTACAACCATCAAGGTTTCATGGTAACTCAGTT
TTCTGACTTAATCAACTACATATCAAATTGGGCAAGATCAAATTCTTTATGGCCTATGACATTTGGATTAGCTTGTTGTG
CTGTAGAAATGATGCACACTGCAGCAAGCAGATATGACCTAGACAGATATGGTGTTATGTTTCGTGCAAGCCCCAGACAA
GCAGATGTTATGATCGTTGCTGGAACACTCACCAACAAAATGGCACCTGCACTACGTAAAGTATACGACCAAATGACAGA
GCCACGTTACGTCATTTCAATGGGAAGCTGTGCGAATGGTGGCGGATATTATCATTATTCATATTCAGTAGTTAGAGGAT
GTGACCGCATAGTACCAGTAGATATTTATGTTCCAGGGTGCCCACCAACAGCAGAAGCATTATTATACGGAATATTTTGT
CTTCAACAAAAAATCAATAGAGGGAATACTTCTATTACAAGAAAATCTACACAAGACTAG

Upstream 100 bases:

>100_bases
CATAAATGGTTTTTGGTCTATGATGGTTTTTTTGGCTATATTAACAGTAGGTTTTATATATGAGTGGAGCAAAGGTGCAC
TAGAATGGGAGTAAACTAGT

Downstream 100 bases:

>100_bases
CATATAATACAATACTAAAATTTATTGTTAACATAATGCCATTATAGCTGTCATATGTAAGCACCAGTCAAATTTTGATC
AAAAATAATATTTACACAAT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MINKNDSVLNNQFWQSYNHQGFMVTQFSDLINYISNWARSNSLWPMTFGLACCAVEMMHTAASRYDLDRYGVMFRASPRQ
ADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIFC
LQQKINRGNTSITRKSTQD

Sequences:

>Translated_179_residues
MINKNDSVLNNQFWQSYNHQGFMVTQFSDLINYISNWARSNSLWPMTFGLACCAVEMMHTAASRYDLDRYGVMFRASPRQ
ADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIFC
LQQKINRGNTSITRKSTQD
>Mature_179_residues
MINKNDSVLNNQFWQSYNHQGFMVTQFSDLINYISNWARSNSLWPMTFGLACCAVEMMHTAASRYDLDRYGVMFRASPRQ
ADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIFC
LQQKINRGNTSITRKSTQD

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=82.1917808219178, Blast_Score=261, Evalue=2e-70,
Organism=Escherichia coli, GI1788624, Length=171, Percent_Identity=45.0292397660819, Blast_Score=167, Evalue=3e-43,
Organism=Escherichia coli, GI1789074, Length=132, Percent_Identity=40.1515151515151, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=39.0243902439024, Blast_Score=97, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=164, Percent_Identity=64.6341463414634, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI18859983, Length=147, Percent_Identity=78.2312925170068, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24642371, Length=147, Percent_Identity=78.2312925170068, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=81.4285714285714, Blast_Score=250, Evalue=3e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_EHRCR (Q2GG48)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507585.1
- ProteinModelPortal:   Q2GG48
- SMR:   Q2GG48
- STRING:   Q2GG48
- GeneID:   3927442
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0787
- TIGR:   ECH_0787
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   HYDNERF
- PhylomeDB:   Q2GG48
- ProtClustDB:   PRK06411
- BioCyc:   ECHA205920:ECH_0787-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20232; Mature: 20232

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKNDSVLNNQFWQSYNHQGFMVTQFSDLINYISNWARSNSLWPMTFGLACCAVEMMHT
CCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AASRYDLDRYGVMFRASPRQADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANG
HHHHCCHHHHCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIFCLQQKINRGNTSITRKSTQD
CCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MINKNDSVLNNQFWQSYNHQGFMVTQFSDLINYISNWARSNSLWPMTFGLACCAVEMMHT
CCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AASRYDLDRYGVMFRASPRQADVMIVAGTLTNKMAPALRKVYDQMTEPRYVISMGSCANG
HHHHCCHHHHCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIFCLQQKINRGNTSITRKSTQD
CCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA