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The map label for this gene is pleD [H]

Identifier: 88657972

GI number: 88657972

Start: 784256

End: 785632

Strand: Direct

Name: pleD [H]

Synonym: ECH_0773

Alternate gene names: 88657972

Gene position: 784256-785632 (Clockwise)

Preceding gene: 88658662

Following gene: 88658458

Centisome position: 66.67

GC content: 31.88

Gene sequence:

>1377_bases
ATGACTGCAAAAGTATTAATAGTAGATGACTTACCTGCAAATATAAAATTGTTGCAAGCTAAGCTCATGAGTGAATATTA
TAACGTTCTAACAGCAACAAATGGGATGGATGCAATTAAAATTGCTGAAGAAAAACATCCAGACATAATACTATTAGATG
TTATGATGCCAGAAATGGATGGATATGAAACTTGCAAGAGATTAAAATCTAATCCAGAAACTACATATATTCCAATAGTC
ATGGTTACAGCTCTTGATAATACATCAGACAATAGAGTTAGTGGTCTCAGCTATGGCGCAGATGATTTCTTAACAAAACC
TATAAATGACACAGCTTTATTTGCACGTATCAGATCATTAACAAGATTCAAGATGGTTATAGACGAATTACGATTAAGAG
GAAAAACAAATGCAGATATGAGTTTATTACACAATAATATGATGGACTATGCAAATAAAATTGACGATGCTAGCATCTTA
ATTGTTGATGAAGATATGATAAGATCTGAGCATATAAATAACGTTTTACAAAAGTCTTTCCAACAAACTGTAGTTTCACA
TAATATAGATCAAGCATTAACCCTAAGTAATACACAAAACTATGACTTAATCATTATTGATCTAGAATTTTCTGGAGATG
GATTGAGGTTGTGCTCACAACTACGTAACAAAATAGAAACACGCTATACTCCTATTCTAGTATTACTTGATGAAAATGAG
GATCCAAAATTATTAAGTAAAGCTTTTGACATAGGAATCCACGACTACATTACAAACCCTATAGACAGTAACGAGCTTAT
TGCAAGGGTAAACGTACAGGTAAAAAGGAAGAGATATCAAGATGCTTTAAGAATCAATGTAGATAACAGCATGGCAATGG
CTATAACTGATCCTCTCACAGGGTGCTATAACAGACGATACTTTGATATGCATTTTCATAACATAGTAAATGAATCACTA
CAAAAAGATAAAAATCTATCTTTAATGATATTAGATATTGACCATTTTAAAGAAGTCAATGATACATTTGGACATACTGT
TGGAGATGAGCTATTGCAACAGTTTAAAAAACGTGTATCAGACAATATACGCATTACAGACCTATTAGCAAGATTTGGTG
GAGAAGAATTTGTTGTAATATTACCAGATACAACAATTACTACAGCAGAACAAATAGCTACAAGAATTCTTTCTGTAATA
GAAGAAACTCCTTTTAAAACAAGTATAGGTGATATAAACAAAACCGTCAGTATTGGTGTAGTTGAAGTACAACAGTCTGA
CATTAATAATATAAAACAACTAGTAGACAGGGCTGATAAGTGCCTATATGAAGCAAAAAATACAGGAAGAAACAAAGTTG
TCACATATCTATCATAG

Upstream 100 bases:

>100_bases
AACAAAATGATAAAGTGACTATTAAAGAAATAGTAGGAAATGATGGAAAAGTATATAAAAGTTATGAAATAAAAAAAATA
TCAAGTAAAAAAGAAGACAT

Downstream 100 bases:

>100_bases
ACAAAACGTTGTTTATAGTACTTGTACTTTGTAAATCATATTTGATTTACAAAGTACCTACACATATCACATATCACATA
TCACATATCACATATCACAT

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTAKVLIVDDLPANIKLLQAKLMSEYYNVLTATNGMDAIKIAEEKHPDIILLDVMMPEMDGYETCKRLKSNPETTYIPIV
MVTALDNTSDNRVSGLSYGADDFLTKPINDTALFARIRSLTRFKMVIDELRLRGKTNADMSLLHNNMMDYANKIDDASIL
IVDEDMIRSEHINNVLQKSFQQTVVSHNIDQALTLSNTQNYDLIIIDLEFSGDGLRLCSQLRNKIETRYTPILVLLDENE
DPKLLSKAFDIGIHDYITNPIDSNELIARVNVQVKRKRYQDALRINVDNSMAMAITDPLTGCYNRRYFDMHFHNIVNESL
QKDKNLSLMILDIDHFKEVNDTFGHTVGDELLQQFKKRVSDNIRITDLLARFGGEEFVVILPDTTITTAEQIATRILSVI
EETPFKTSIGDINKTVSIGVVEVQQSDINNIKQLVDRADKCLYEAKNTGRNKVVTYLS

Sequences:

>Translated_458_residues
MTAKVLIVDDLPANIKLLQAKLMSEYYNVLTATNGMDAIKIAEEKHPDIILLDVMMPEMDGYETCKRLKSNPETTYIPIV
MVTALDNTSDNRVSGLSYGADDFLTKPINDTALFARIRSLTRFKMVIDELRLRGKTNADMSLLHNNMMDYANKIDDASIL
IVDEDMIRSEHINNVLQKSFQQTVVSHNIDQALTLSNTQNYDLIIIDLEFSGDGLRLCSQLRNKIETRYTPILVLLDENE
DPKLLSKAFDIGIHDYITNPIDSNELIARVNVQVKRKRYQDALRINVDNSMAMAITDPLTGCYNRRYFDMHFHNIVNESL
QKDKNLSLMILDIDHFKEVNDTFGHTVGDELLQQFKKRVSDNIRITDLLARFGGEEFVVILPDTTITTAEQIATRILSVI
EETPFKTSIGDINKTVSIGVVEVQQSDINNIKQLVDRADKCLYEAKNTGRNKVVTYLS
>Mature_457_residues
TAKVLIVDDLPANIKLLQAKLMSEYYNVLTATNGMDAIKIAEEKHPDIILLDVMMPEMDGYETCKRLKSNPETTYIPIVM
VTALDNTSDNRVSGLSYGADDFLTKPINDTALFARIRSLTRFKMVIDELRLRGKTNADMSLLHNNMMDYANKIDDASILI
VDEDMIRSEHINNVLQKSFQQTVVSHNIDQALTLSNTQNYDLIIIDLEFSGDGLRLCSQLRNKIETRYTPILVLLDENED
PKLLSKAFDIGIHDYITNPIDSNELIARVNVQVKRKRYQDALRINVDNSMAMAITDPLTGCYNRRYFDMHFHNIVNESLQ
KDKNLSLMILDIDHFKEVNDTFGHTVGDELLQQFKKRVSDNIRITDLLARFGGEEFVVILPDTTITTAEQIATRILSVIE
ETPFKTSIGDINKTVSIGVVEVQQSDINNIKQLVDRADKCLYEAKNTGRNKVVTYLS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=177, Percent_Identity=41.8079096045198, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786584, Length=189, Percent_Identity=32.8042328042328, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=36.0248447204969, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787802, Length=217, Percent_Identity=31.7972350230415, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=37.9518072289157, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=35.4037267080745, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=36.4779874213836, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=31.25, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=34.9112426035503, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=182, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788956, Length=176, Percent_Identity=31.8181818181818, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=36.9747899159664, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=38.8429752066116, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=33.1288343558282, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=36.0655737704918, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788394, Length=153, Percent_Identity=31.3725490196078, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082012, Length=119, Percent_Identity=31.9327731092437, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI2367329, Length=119, Percent_Identity=42.8571428571429, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=104, Percent_Identity=38.4615384615385, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=109, Percent_Identity=34.8623853211009, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=106, Percent_Identity=38.6792452830189, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790860, Length=119, Percent_Identity=31.9327731092437, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790552, Length=122, Percent_Identity=34.4262295081967, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=31.6666666666667, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=155, Percent_Identity=29.6774193548387, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081977, Length=162, Percent_Identity=30.8641975308642, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 52049; Mature: 51918

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKVLIVDDLPANIKLLQAKLMSEYYNVLTATNGMDAIKIAEEKHPDIILLDVMMPEMD
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEEECCCCC
GYETCKRLKSNPETTYIPIVMVTALDNTSDNRVSGLSYGADDFLTKPINDTALFARIRSL
CHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHH
TRFKMVIDELRLRGKTNADMSLLHNNMMDYANKIDDASILIVDEDMIRSEHINNVLQKSF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHH
QQTVVSHNIDQALTLSNTQNYDLIIIDLEFSGDGLRLCSQLRNKIETRYTPILVLLDENE
HHHHHHCCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCC
DPKLLSKAFDIGIHDYITNPIDSNELIARVNVQVKRKRYQDALRINVDNSMAMAITDPLT
CHHHHHHHHHCCHHHHHCCCCCCCCEEEEECHHHHHHHHCCCEEEECCCCEEEEEECCHH
GCYNRRYFDMHFHNIVNESLQKDKNLSLMILDIDHFKEVNDTFGHTVGDELLQQFKKRVS
HHHCCEEEEHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHC
DNIRITDLLARFGGEEFVVILPDTTITTAEQIATRILSVIEETPFKTSIGDINKTVSIGV
CCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
VEVQQSDINNIKQLVDRADKCLYEAKNTGRNKVVTYLS
EEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
TAKVLIVDDLPANIKLLQAKLMSEYYNVLTATNGMDAIKIAEEKHPDIILLDVMMPEMD
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEEECCCCC
GYETCKRLKSNPETTYIPIVMVTALDNTSDNRVSGLSYGADDFLTKPINDTALFARIRSL
CHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHH
TRFKMVIDELRLRGKTNADMSLLHNNMMDYANKIDDASILIVDEDMIRSEHINNVLQKSF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHH
QQTVVSHNIDQALTLSNTQNYDLIIIDLEFSGDGLRLCSQLRNKIETRYTPILVLLDENE
HHHHHHCCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCC
DPKLLSKAFDIGIHDYITNPIDSNELIARVNVQVKRKRYQDALRINVDNSMAMAITDPLT
CHHHHHHHHHCCHHHHHCCCCCCCCEEEEECHHHHHHHHCCCEEEECCCCEEEEEECCHH
GCYNRRYFDMHFHNIVNESLQKDKNLSLMILDIDHFKEVNDTFGHTVGDELLQQFKKRVS
HHHCCEEEEHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHC
DNIRITDLLARFGGEEFVVILPDTTITTAEQIATRILSVIEETPFKTSIGDINKTVSIGV
CCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
VEVQQSDINNIKQLVDRADKCLYEAKNTGRNKVVTYLS
EEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA