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The map label for this gene is prsA [H]

Identifier: 88657930

GI number: 88657930

Start: 1048066

End: 1049022

Strand: Direct

Name: prsA [H]

Synonym: ECH_1024

Alternate gene names: 88657930

Gene position: 1048066-1049022 (Clockwise)

Preceding gene: 88657961

Following gene: 88658453

Centisome position: 89.1

GC content: 29.68

Gene sequence:

>957_bases
ATGTTAATATCTTCTGGTACATCTAGTATTAACTTAGCAAAGTGCATATCAAACATTACAAGTATAAAGTTAGTTAACTC
TTGTATTTCTCGTTTTTCTGATCAAGAATTAAACGTAGAAATTCAAGAAAACCAAGATGACAAAAATAAACACGTCATAA
TTATAAATTCACTTTGTTCCCCAGCACATGATAATTTACTAGAATTACTCTTGTTAACGGATGCAATTAATAGAACATTG
CATCCTAGAAAAATAACCATGATAATACCTTATCTTTGTTACACTAGACAAGACAGAGTAATGTATAGAAACTTAGATGA
CAACAGCCTAATGGTCTCTGCATTAAGTGCAAAAGTTATCATTAACATATTAAGAACAGCAAACATTAACAACATTATAT
TCATTGATCTACATTCAAATCAATTATCTGGTTTCTTTGACATAGCAACTACTAATTTAAGTTCCCACACTGTTTTCATA
GGAGATATTATAGAAAAACATAATACTAACAACTTAGTTGTTGTATCTCCTGACTATGGAGCATTAAACAGAACTCGAAC
TTTTGCAAACGTATTATCAAAACAGTGCAAATTACACAACGAAATTCAAGTAGCAGTAATAGATAAATACAGAGCAAAAC
CTGGTGTATCTGAAGTAATGAACATAACTGGTAATGTAGAAAACAAAGATTGTATAATAGTAGATGATATTGTAGATTCA
GCTGGAACATTATGCAATGCGGCATCAGCTTTAAAAGACAGGGGAGCTTTACAAGTTAGTGCATATGTTACACATGGAAT
ATTATCTGGCAATGCAGTAGAAAAAGTTACGAATTCAAAACTTGATAATTTAATAATAACAGACACAATACATAATGGTT
TCTTCAACACATCAAAAATCCGAATTTTATCCATTGATAAATTTTTAAGCAACTATATACTGTATAATTTAGTATAA

Upstream 100 bases:

>100_bases
GATTACCCTATGATAACACATATTATTAAAAAATACCTATTTACTTTTAGTAAACCATTACGTATTATCAGGACATCCAT
TTTACATATAGATTTTTTCG

Downstream 100 bases:

>100_bases
GTTTAGGAATAAATTGATAGAAGATAAATTTGAAAATAGTACACATTCAAAAACAAGCAAGTCATTAACTAAAAATGACA
TATTGCAAGCTGCAAAGTTA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MLISSGTSSINLAKCISNITSIKLVNSCISRFSDQELNVEIQENQDDKNKHVIIINSLCSPAHDNLLELLLLTDAINRTL
HPRKITMIIPYLCYTRQDRVMYRNLDDNSLMVSALSAKVIINILRTANINNIIFIDLHSNQLSGFFDIATTNLSSHTVFI
GDIIEKHNTNNLVVVSPDYGALNRTRTFANVLSKQCKLHNEIQVAVIDKYRAKPGVSEVMNITGNVENKDCIIVDDIVDS
AGTLCNAASALKDRGALQVSAYVTHGILSGNAVEKVTNSKLDNLIITDTIHNGFFNTSKIRILSIDKFLSNYILYNLV

Sequences:

>Translated_318_residues
MLISSGTSSINLAKCISNITSIKLVNSCISRFSDQELNVEIQENQDDKNKHVIIINSLCSPAHDNLLELLLLTDAINRTL
HPRKITMIIPYLCYTRQDRVMYRNLDDNSLMVSALSAKVIINILRTANINNIIFIDLHSNQLSGFFDIATTNLSSHTVFI
GDIIEKHNTNNLVVVSPDYGALNRTRTFANVLSKQCKLHNEIQVAVIDKYRAKPGVSEVMNITGNVENKDCIIVDDIVDS
AGTLCNAASALKDRGALQVSAYVTHGILSGNAVEKVTNSKLDNLIITDTIHNGFFNTSKIRILSIDKFLSNYILYNLV
>Mature_318_residues
MLISSGTSSINLAKCISNITSIKLVNSCISRFSDQELNVEIQENQDDKNKHVIIINSLCSPAHDNLLELLLLTDAINRTL
HPRKITMIIPYLCYTRQDRVMYRNLDDNSLMVSALSAKVIINILRTANINNIIFIDLHSNQLSGFFDIATTNLSSHTVFI
GDIIEKHNTNNLVVVSPDYGALNRTRTFANVLSKQCKLHNEIQVAVIDKYRAKPGVSEVMNITGNVENKDCIIVDDIVDS
AGTLCNAASALKDRGALQVSAYVTHGILSGNAVEKVTNSKLDNLIITDTIHNGFFNTSKIRILSIDKFLSNYILYNLV

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=316, Percent_Identity=32.5949367088608, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI84875539, Length=316, Percent_Identity=32.2784810126582, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=33.4394904458599, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=32.8025477707006, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4506133, Length=335, Percent_Identity=25.0746268656716, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI194018537, Length=334, Percent_Identity=26.6467065868263, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=38.0952380952381, Blast_Score=221, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17554702, Length=312, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71989924, Length=312, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17570245, Length=326, Percent_Identity=25.1533742331288, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320946, Length=286, Percent_Identity=35.3146853146853, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321776, Length=306, Percent_Identity=33.9869281045752, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6319403, Length=287, Percent_Identity=35.191637630662, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=69, Percent_Identity=49.2753623188406, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322667, Length=188, Percent_Identity=28.1914893617021, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355239, Length=287, Percent_Identity=34.4947735191638, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45551540, Length=302, Percent_Identity=32.7814569536424, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24651458, Length=342, Percent_Identity=27.1929824561404, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24651456, Length=342, Percent_Identity=27.1929824561404, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281362873, Length=342, Percent_Identity=27.1929824561404, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24651454, Length=342, Percent_Identity=27.1929824561404, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24651462, Length=361, Percent_Identity=25.7617728531856, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24651464, Length=361, Percent_Identity=25.7617728531856, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45552010, Length=361, Percent_Identity=25.7617728531856, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35278; Mature: 35278

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISSGTSSINLAKCISNITSIKLVNSCISRFSDQELNVEIQENQDDKNKHVIIINSLCS
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCC
PAHDNLLELLLLTDAINRTLHPRKITMIIPYLCYTRQDRVMYRNLDDNSLMVSALSAKVI
CCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCEEEECCCCCCEEHHHHHHHHH
INILRTANINNIIFIDLHSNQLSGFFDIATTNLSSHTVFIGDIIEKHNTNNLVVVSPDYG
HHHHHHCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEEEEEHHHHCCCCCEEEECCCCC
ALNRTRTFANVLSKQCKLHNEIQVAVIDKYRAKPGVSEVMNITGNVENKDCIIVDDIVDS
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEEHHHCC
AGTLCNAASALKDRGALQVSAYVTHGILSGNAVEKVTNSKLDNLIITDTIHNGFFNTSKI
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCEE
RILSIDKFLSNYILYNLV
EEEEHHHHHHHHHHEECC
>Mature Secondary Structure
MLISSGTSSINLAKCISNITSIKLVNSCISRFSDQELNVEIQENQDDKNKHVIIINSLCS
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCC
PAHDNLLELLLLTDAINRTLHPRKITMIIPYLCYTRQDRVMYRNLDDNSLMVSALSAKVI
CCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCEEEECCCCCCEEHHHHHHHHH
INILRTANINNIIFIDLHSNQLSGFFDIATTNLSSHTVFIGDIIEKHNTNNLVVVSPDYG
HHHHHHCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEEEEEHHHHCCCCCEEEECCCCC
ALNRTRTFANVLSKQCKLHNEIQVAVIDKYRAKPGVSEVMNITGNVENKDCIIVDDIVDS
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEEHHHCC
AGTLCNAASALKDRGALQVSAYVTHGILSGNAVEKVTNSKLDNLIITDTIHNGFFNTSKI
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCEE
RILSIDKFLSNYILYNLV
EEEEHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]