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The map label for this gene is thiD [H]

Identifier: 88657886

GI number: 88657886

Start: 938334

End: 939143

Strand: Direct

Name: thiD [H]

Synonym: ECH_0914

Alternate gene names: 88657886

Gene position: 938334-939143 (Clockwise)

Preceding gene: 88658126

Following gene: 88657686

Centisome position: 79.77

GC content: 32.35

Gene sequence:

>810_bases
ATGACTAAACATCAATATAAAGGTCGAGTATTAACTATAGCCGGTTCTGATTCAGGAGGTGGTGCTGGTATCCAAGCAGA
TATCAAAACTATATCAGCATTGGGATGTTATGCTGCAAGTTGTATTACATCAGTAACAGCACAGAATACAACTCAAGTTT
ACAGTGTATATAATATGCCACAACATATTATCCAGCAACAAATAGAAGTTGTTTTATCTGACATTAATATAGACACCATT
AAAATTGGTATGTTACCTTCCTCTAAAGCAATAAAAGCAGTAGCACAATCCTTGCCAGATATCCCTATTATTGTAGATCC
AGTAATGGTATCGGCATCAAATTTTAGATTAATGGACAGTAGTGCAATTTCTGACTTTATAGAATATATAATTCCAAAAA
CAACAATAATTACACCAAATATACCAGAAGCAGAAGCTTTAGCTCAAATTGAGATAAAAGATCAAAATGACATGACAAAA
GCAAGTAAGATAATAAAATCACTTGGGACTAAACACGTTCTGATTAAAGGAGGACACATCAATCAAGAGATTATAAATAA
CATTTTACTAACAGAAGAAAATCAAATTATAAATTTCTCACATAAAAGAATATCTAAGAAGGAATTACATGGCACAGGTT
GTACTCTATCGTCAGCCATTGCAAGTTTTATAGCACAGAAGATGTCAATACAAGAAAGTGTAAATCTAGCAATCCAATAT
ATCCTCAATACCATAAAGATAGTACCACAAATAGGAAAAGGCAATAATCCTGTCTTCCATAACTACAATACAATCAGTAG
TGACCTTTAA

Upstream 100 bases:

>100_bases
TTTCAAAATTTGAGCAATATAGTAAAATGCATAGACAGAAACCTACCTTACTATAATAATGGCGTCACTATACCATTTGT
AAAATCTATAATAGAAAAAA

Downstream 100 bases:

>100_bases
TTTAAAAAAATCTATAACTTTTATATAGAGAAAATTTCATTTTTACACTGTCAGTTTATAGCTCTTAATTTAACGAAATA
ACTCAGGTCTTATCTGTCAC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTKHQYKGRVLTIAGSDSGGGAGIQADIKTISALGCYAASCITSVTAQNTTQVYSVYNMPQHIIQQQIEVVLSDINIDTI
KIGMLPSSKAIKAVAQSLPDIPIIVDPVMVSASNFRLMDSSAISDFIEYIIPKTTIITPNIPEAEALAQIEIKDQNDMTK
ASKIIKSLGTKHVLIKGGHINQEIINNILLTEENQIINFSHKRISKKELHGTGCTLSSAIASFIAQKMSIQESVNLAIQY
ILNTIKIVPQIGKGNNPVFHNYNTISSDL

Sequences:

>Translated_269_residues
MTKHQYKGRVLTIAGSDSGGGAGIQADIKTISALGCYAASCITSVTAQNTTQVYSVYNMPQHIIQQQIEVVLSDINIDTI
KIGMLPSSKAIKAVAQSLPDIPIIVDPVMVSASNFRLMDSSAISDFIEYIIPKTTIITPNIPEAEALAQIEIKDQNDMTK
ASKIIKSLGTKHVLIKGGHINQEIINNILLTEENQIINFSHKRISKKELHGTGCTLSSAIASFIAQKMSIQESVNLAIQY
ILNTIKIVPQIGKGNNPVFHNYNTISSDL
>Mature_268_residues
TKHQYKGRVLTIAGSDSGGGAGIQADIKTISALGCYAASCITSVTAQNTTQVYSVYNMPQHIIQQQIEVVLSDINIDTIK
IGMLPSSKAIKAVAQSLPDIPIIVDPVMVSASNFRLMDSSAISDFIEYIIPKTTIITPNIPEAEALAQIEIKDQNDMTKA
SKIIKSLGTKHVLIKGGHINQEIINNILLTEENQIINFSHKRISKKELHGTGCTLSSAIASFIAQKMSIQESVNLAIQYI
LNTIKIVPQIGKGNNPVFHNYNTISSDL

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=257, Percent_Identity=38.5214007782101, Blast_Score=181, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6325378, Length=270, Percent_Identity=35.1851851851852, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324997, Length=271, Percent_Identity=38.0073800738007, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=38.2352941176471, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29173; Mature: 29042

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHQYKGRVLTIAGSDSGGGAGIQADIKTISALGCYAASCITSVTAQNTTQVYSVYNMP
CCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHCCH
QHIIQQQIEVVLSDINIDTIKIGMLPSSKAIKAVAQSLPDIPIIVDPVMVSASNFRLMDS
HHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECCEEECCCCEEEECH
SAISDFIEYIIPKTTIITPNIPEAEALAQIEIKDQNDMTKASKIIKSLGTKHVLIKGGHI
HHHHHHHHHHCCCCEEECCCCCCHHHEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCC
NQEIINNILLTEENQIINFSHKRISKKELHGTGCTLSSAIASFIAQKMSIQESVNLAIQY
CHHHHHHHEECCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILNTIKIVPQIGKGNNPVFHNYNTISSDL
HHHHHHHHCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
TKHQYKGRVLTIAGSDSGGGAGIQADIKTISALGCYAASCITSVTAQNTTQVYSVYNMP
CCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHCCH
QHIIQQQIEVVLSDINIDTIKIGMLPSSKAIKAVAQSLPDIPIIVDPVMVSASNFRLMDS
HHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECCEEECCCCEEEECH
SAISDFIEYIIPKTTIITPNIPEAEALAQIEIKDQNDMTKASKIIKSLGTKHVLIKGGHI
HHHHHHHHHHCCCCEEECCCCCCHHHEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCC
NQEIINNILLTEENQIINFSHKRISKKELHGTGCTLSSAIASFIAQKMSIQESVNLAIQY
CHHHHHHHEECCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILNTIKIVPQIGKGNNPVFHNYNTISSDL
HHHHHHHHCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA