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The map label for this gene is lysC [H]

Identifier: 88657747

GI number: 88657747

Start: 1026178

End: 1027431

Strand: Direct

Name: lysC [H]

Synonym: ECH_1001

Alternate gene names: 88657747

Gene position: 1026178-1027431 (Clockwise)

Preceding gene: 88658567

Following gene: 88658348

Centisome position: 87.24

GC content: 31.9

Gene sequence:

>1254_bases
ATGAAGAGGATTTTAGTAAAAAAATTTGGAGGAACTTCTTTACAAGACATAGAATGCATTAATAGAGTTGCAGAAATAAT
AAAACAAGATGTTAACAATAATTATAAAGTAGTTGTAGTAGTATCAGCTATGGGAAAATTCACTGATAACATCATTTCAC
AAATTAAACAAATTTCCGATGTCAAATCTCAATCTGAACGCTCCGAATATGATCTAATCATTTCTTCAGGAGAACAAATA
TCATGCGGACTATTATCATTAGCTCTACAAAAAATAGGAATCAATGCTCAATCATGGTTAGGATGGCAACTACCAATAGT
AACAACTGAAGACCATACTAAAGCAAGAATCATAGATATTAACACATGTTCACTACAGGATTCACTAGCTAATAATGATG
TTGCTATTGTGGCTGGATTTCAGGGAATGCATAAAAACAATAGAGTAACAACCTTAGGCAGAGGAGGTTCTGACACTTCA
GCTGTAGCAATTGCAGCAGCACTAAAAGTAGATTTATGCTACATTTACACAGATGTAGACGGAATATATACAGCAGATCC
TAATGTGGTACCAAAAGCACGCAAATTAGATTACATTACATATGATGAAATGATAGAGATGTCTTCTCTTGGCGCTAAAG
TATTACAAGTACGTTCAGTAGAAATAGCAATGAAATATAACATAAAATTGTGTATATTATCTACTTTTAATCCTGGAAAA
GGGACAATCTTACGCAAAAAAGGAGAATCAGATATGGAAAGTCAATTAATTACTGGGGTTACATGTAATAACAAAACAGC
AAGTATTACACTAAAAGAGGTAAAAGCAATATCTGGCGTTACCACAGTATTTAATGCAATAGCAGAAAAAAACATTAACG
TCGATATGATCATTCAAAGTGTGAATGATAACAATGCAAATGATATCACTTTTACAATTTCAGAAGAAGATTTGCCAACA
ACAACAAAGTTTTTAACAGAAATTCAAACTGAACTTATGTATCAGGATTTAATAATCAATTCCGAAGTTGCAAAAGTTTC
CATTATTGGAGTAGGCATGATTTCTCATTCTGGAGTAGCTTACAAAATGTTTGATACTTTAACATCTAATAATATAAAAA
TATTAGCAGTTACTACTTCAGAGATAAAAATCAGCGTTCTAATATCGAGAAAAGACAGCCAACTTGCAACAATAGCATTG
CACTCTACTTTTGGACTTGATAACACAGAATCAGATTTACACATAATAAGTTAA

Upstream 100 bases:

>100_bases
ATTATATAATAAAGAATTTAACTGTATTAATACAAACTTTGCAAAATATAAAAAATCACTTATCATTTAATTAACAGTCA
TATATTTCTAGCACACAACA

Downstream 100 bases:

>100_bases
TCTATTTATAATATAGCTTATAACCCTTCTGGTGTTTAGATAACGTATTACATATAAGTAATATAGTTTTAAGAATTATT
AAACAACAATAAACGTAGCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MKRILVKKFGGTSLQDIECINRVAEIIKQDVNNNYKVVVVVSAMGKFTDNIISQIKQISDVKSQSERSEYDLIISSGEQI
SCGLLSLALQKIGINAQSWLGWQLPIVTTEDHTKARIIDINTCSLQDSLANNDVAIVAGFQGMHKNNRVTTLGRGGSDTS
AVAIAAALKVDLCYIYTDVDGIYTADPNVVPKARKLDYITYDEMIEMSSLGAKVLQVRSVEIAMKYNIKLCILSTFNPGK
GTILRKKGESDMESQLITGVTCNNKTASITLKEVKAISGVTTVFNAIAEKNINVDMIIQSVNDNNANDITFTISEEDLPT
TTKFLTEIQTELMYQDLIINSEVAKVSIIGVGMISHSGVAYKMFDTLTSNNIKILAVTTSEIKISVLISRKDSQLATIAL
HSTFGLDNTESDLHIIS

Sequences:

>Translated_417_residues
MKRILVKKFGGTSLQDIECINRVAEIIKQDVNNNYKVVVVVSAMGKFTDNIISQIKQISDVKSQSERSEYDLIISSGEQI
SCGLLSLALQKIGINAQSWLGWQLPIVTTEDHTKARIIDINTCSLQDSLANNDVAIVAGFQGMHKNNRVTTLGRGGSDTS
AVAIAAALKVDLCYIYTDVDGIYTADPNVVPKARKLDYITYDEMIEMSSLGAKVLQVRSVEIAMKYNIKLCILSTFNPGK
GTILRKKGESDMESQLITGVTCNNKTASITLKEVKAISGVTTVFNAIAEKNINVDMIIQSVNDNNANDITFTISEEDLPT
TTKFLTEIQTELMYQDLIINSEVAKVSIIGVGMISHSGVAYKMFDTLTSNNIKILAVTTSEIKISVLISRKDSQLATIAL
HSTFGLDNTESDLHIIS
>Mature_417_residues
MKRILVKKFGGTSLQDIECINRVAEIIKQDVNNNYKVVVVVSAMGKFTDNIISQIKQISDVKSQSERSEYDLIISSGEQI
SCGLLSLALQKIGINAQSWLGWQLPIVTTEDHTKARIIDINTCSLQDSLANNDVAIVAGFQGMHKNNRVTTLGRGGSDTS
AVAIAAALKVDLCYIYTDVDGIYTADPNVVPKARKLDYITYDEMIEMSSLGAKVLQVRSVEIAMKYNIKLCILSTFNPGK
GTILRKKGESDMESQLITGVTCNNKTASITLKEVKAISGVTTVFNAIAEKNINVDMIIQSVNDNNANDITFTISEEDLPT
TTKFLTEIQTELMYQDLIINSEVAKVSIIGVGMISHSGVAYKMFDTLTSNNIKILAVTTSEIKISVLISRKDSQLATIAL
HSTFGLDNTESDLHIIS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=465, Percent_Identity=27.741935483871, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790455, Length=458, Percent_Identity=27.0742358078603, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1790376, Length=286, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320893, Length=420, Percent_Identity=25.2380952380952, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 45576; Mature: 45576

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILVKKFGGTSLQDIECINRVAEIIKQDVNNNYKVVVVVSAMGKFTDNIISQIKQISD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHH
VKSQSERSEYDLIISSGEQISCGLLSLALQKIGINAQSWLGWQLPIVTTEDHTKARIIDI
HHHHCCCCCEEEEEECCCEEEHHHHHHHHHHHCCCHHHCCCEEEEEEECCCCCCEEEEEE
NTCSLQDSLANNDVAIVAGFQGMHKNNRVTTLGRGGSDTSAVAIAAALKVDLCYIYTDVD
EEECCCHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHEEEEEEEEEEEEEEEECC
GIYTADPNVVPKARKLDYITYDEMIEMSSLGAKVLQVRSVEIAMKYNIKLCILSTFNPGK
CEEECCCCCCCCHHHEEEEEHHHHHHHHHCCCEEEEEEEEEEEEEEEEEEEEEEECCCCC
GTILRKKGESDMESQLITGVTCNNKTASITLKEVKAISGVTTVFNAIAEKNINVDMIIQS
CCEEEECCCCCCHHHEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VNDNNANDITFTISEEDLPTTTKFLTEIQTELMYQDLIINSEVAKVSIIGVGMISHSGVA
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEEEECCCEE
YKMFDTLTSNNIKILAVTTSEIKISVLISRKDSQLATIALHSTFGLDNTESDLHIIS
EEEEHHHCCCCEEEEEEEECCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEEC
>Mature Secondary Structure
MKRILVKKFGGTSLQDIECINRVAEIIKQDVNNNYKVVVVVSAMGKFTDNIISQIKQISD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHH
VKSQSERSEYDLIISSGEQISCGLLSLALQKIGINAQSWLGWQLPIVTTEDHTKARIIDI
HHHHCCCCCEEEEEECCCEEEHHHHHHHHHHHCCCHHHCCCEEEEEEECCCCCCEEEEEE
NTCSLQDSLANNDVAIVAGFQGMHKNNRVTTLGRGGSDTSAVAIAAALKVDLCYIYTDVD
EEECCCHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHEEEEEEEEEEEEEEEECC
GIYTADPNVVPKARKLDYITYDEMIEMSSLGAKVLQVRSVEIAMKYNIKLCILSTFNPGK
CEEECCCCCCCCHHHEEEEEHHHHHHHHHCCCEEEEEEEEEEEEEEEEEEEEEEECCCCC
GTILRKKGESDMESQLITGVTCNNKTASITLKEVKAISGVTTVFNAIAEKNINVDMIIQS
CCEEEECCCCCCHHHEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VNDNNANDITFTISEEDLPTTTKFLTEIQTELMYQDLIINSEVAKVSIIGVGMISHSGVA
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEEEECCCEE
YKMFDTLTSNNIKILAVTTSEIKISVLISRKDSQLATIALHSTFGLDNTESDLHIIS
EEEEHHHCCCCEEEEEEEECCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]