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The map label for this gene is gatA [H]

Identifier: 88657601

GI number: 88657601

Start: 712452

End: 713915

Strand: Direct

Name: gatA [H]

Synonym: ECH_0703

Alternate gene names: 88657601

Gene position: 712452-713915 (Clockwise)

Preceding gene: 88657806

Following gene: 88658655

Centisome position: 60.57

GC content: 34.02

Gene sequence:

>1464_bases
ATGAAAAACATTTTAAAATTATCTATTGCAGAAATGCATGACAACTTAAAAAAAAGAGAATTCTCCGCTGTAGAATTAAC
TAAACTTCATATTGAAGCAGTTAACAACGAAAAATTAAATGCTTTTATAACCAAAACACCAGAAATTGCTTTAAGTGCAG
CTGAAAAAGCAGATTACATCTTTACACATCAAAAAGAAAATTTAACTCCTTTAACTGGAATTCCAGTGGGTATAAAAGAT
TTATTCTGTACAAAACACGTTAGAACAACAGCATGTTCTAACATATTAAAAAATTTTACCCCTCAATATGATTCAACTGT
TACTAAACGGCTACTTGACAATGGAGCTGTCATGCTAGGAAAGTTAAACATGGATGAATTTGCGATGGGATCATCAAATT
CTAACAGTTGTTTTGGACATGTAAAAAATCCATGGGTAAGAGCAGATGGTGTTGAAGTTGTACCTGGAGGATCATCAGGT
GGTTCAAGTGCTGCTGTAGCAGGATTCTTATGTGCTGGTGCTTTAGGTAGTGATACAGGTGGATCAGTACGTCAACCTGC
AGCACTATGCGGGATAGTAGGGCTGAAACCAACATATGGAAGATGTTCAAGGTTTGGTATGATAGCATTTGCTAGCTCTC
TTGACCAAGCAGGAGTACTGACACGTACAGTAGAAGATGCATCCTTAATGTTACAGTCAATTTGTGGATATGATATTCAA
GATTCAACTTCTGCTAATATAGCTGTCCCTAAATTTTCTGAAAGTATTACACATACAATTAAAGGTAAACGTATTGGCAT
ACCAAAGGAGTATGAATTATCGGGTAAATATCAAGAATATACTGAAGTTTCAGAAATGTGGGCCAAGGGAATACAATATC
TCAAGGATGAAGGAGCTGAAATTATAGAAATTAGTTTACCACATACAAGTTATGCATTACCTGTATATTATATTATATGT
TCTGCAGAAGCATCTTCTAACCTTGCAAGATATGATGGAATAAGGTATGGAGCACGCATATCATCTGATGACATTAATGA
GATGTACGAGTTAACACGTGGACATAATTTTGGAACAGAAGTAAAACGCAGAATATTAATTGGAGCATATGTATTATCAT
CAGGATATTACGACGCATACTATAATAAAGCACAGCGTATCAGACACTTAGTAATAAATGATTTTGTTGAGTCATTTAAA
AAAATTGACTACATACTGACTCCTACTACCCCTAAAGAAGCATTCGCAATAAACGAACAATTAGATACATTAACTATGTA
TTTAAATGATGTATTCACCGTTCCAGCAAGTTTAGCAGGATTACCTGCTATTTCCGTTCCAATAGGATTATCAAAAAGTA
ACTTACCACTTTCTTTACAAGTAATAGGTAACTATTACGATGAGGGAGGAATTTTAAATGTAGCTAGTATTATAGAAAAA
CATACAGGAAAAATTTTAAAATAG

Upstream 100 bases:

>100_bases
CTTTCAGTTTATCAAGTATATTTTTAAATTACCAAAAATTTAATTGTTTTATCGTAGATAATATGGTAATTTACAGCCAC
TCTTATCTACTTACTCAAAC

Downstream 100 bases:

>100_bases
AGGGTAACCATTATGGCTGGTCATTCACAATTTGCAAATATAAAACATAGGAAAGGAGCTCAAGATGCAAAAAGAGCTCA
AAAATTTACAAAATTAATCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKNILKLSIAEMHDNLKKREFSAVELTKLHIEAVNNEKLNAFITKTPEIALSAAEKADYIFTHQKENLTPLTGIPVGIKD
LFCTKHVRTTACSNILKNFTPQYDSTVTKRLLDNGAVMLGKLNMDEFAMGSSNSNSCFGHVKNPWVRADGVEVVPGGSSG
GSSAAVAGFLCAGALGSDTGGSVRQPAALCGIVGLKPTYGRCSRFGMIAFASSLDQAGVLTRTVEDASLMLQSICGYDIQ
DSTSANIAVPKFSESITHTIKGKRIGIPKEYELSGKYQEYTEVSEMWAKGIQYLKDEGAEIIEISLPHTSYALPVYYIIC
SAEASSNLARYDGIRYGARISSDDINEMYELTRGHNFGTEVKRRILIGAYVLSSGYYDAYYNKAQRIRHLVINDFVESFK
KIDYILTPTTPKEAFAINEQLDTLTMYLNDVFTVPASLAGLPAISVPIGLSKSNLPLSLQVIGNYYDEGGILNVASIIEK
HTGKILK

Sequences:

>Translated_487_residues
MKNILKLSIAEMHDNLKKREFSAVELTKLHIEAVNNEKLNAFITKTPEIALSAAEKADYIFTHQKENLTPLTGIPVGIKD
LFCTKHVRTTACSNILKNFTPQYDSTVTKRLLDNGAVMLGKLNMDEFAMGSSNSNSCFGHVKNPWVRADGVEVVPGGSSG
GSSAAVAGFLCAGALGSDTGGSVRQPAALCGIVGLKPTYGRCSRFGMIAFASSLDQAGVLTRTVEDASLMLQSICGYDIQ
DSTSANIAVPKFSESITHTIKGKRIGIPKEYELSGKYQEYTEVSEMWAKGIQYLKDEGAEIIEISLPHTSYALPVYYIIC
SAEASSNLARYDGIRYGARISSDDINEMYELTRGHNFGTEVKRRILIGAYVLSSGYYDAYYNKAQRIRHLVINDFVESFK
KIDYILTPTTPKEAFAINEQLDTLTMYLNDVFTVPASLAGLPAISVPIGLSKSNLPLSLQVIGNYYDEGGILNVASIIEK
HTGKILK
>Mature_487_residues
MKNILKLSIAEMHDNLKKREFSAVELTKLHIEAVNNEKLNAFITKTPEIALSAAEKADYIFTHQKENLTPLTGIPVGIKD
LFCTKHVRTTACSNILKNFTPQYDSTVTKRLLDNGAVMLGKLNMDEFAMGSSNSNSCFGHVKNPWVRADGVEVVPGGSSG
GSSAAVAGFLCAGALGSDTGGSVRQPAALCGIVGLKPTYGRCSRFGMIAFASSLDQAGVLTRTVEDASLMLQSICGYDIQ
DSTSANIAVPKFSESITHTIKGKRIGIPKEYELSGKYQEYTEVSEMWAKGIQYLKDEGAEIIEISLPHTSYALPVYYIIC
SAEASSNLARYDGIRYGARISSDDINEMYELTRGHNFGTEVKRRILIGAYVLSSGYYDAYYNKAQRIRHLVINDFVESFK
KIDYILTPTTPKEAFAINEQLDTLTMYLNDVFTVPASLAGLPAISVPIGLSKSNLPLSLQVIGNYYDEGGILNVASIIEK
HTGKILK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=41.7821782178218, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI195972892, Length=503, Percent_Identity=23.6580516898608, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=454, Percent_Identity=37.0044052863436, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=24.7011952191235, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=246, Percent_Identity=28.8617886178862, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=321, Percent_Identity=26.4797507788162, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=239, Percent_Identity=30.5439330543933, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=239, Percent_Identity=30.5439330543933, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=464, Percent_Identity=37.0689655172414, Blast_Score=259, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6319685, Length=433, Percent_Identity=25.4041570438799, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=200, Percent_Identity=29, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=499, Percent_Identity=40.8817635270541, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24652985, Length=506, Percent_Identity=26.0869565217391, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI19922090, Length=506, Percent_Identity=26.0869565217391, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24652981, Length=506, Percent_Identity=26.0869565217391, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24652983, Length=506, Percent_Identity=26.0869565217391, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161078093, Length=512, Percent_Identity=27.1484375, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=24.3137254901961, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI45550774, Length=207, Percent_Identity=30.9178743961353, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648435, Length=207, Percent_Identity=30.9178743961353, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648437, Length=207, Percent_Identity=30.9178743961353, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356731, Length=239, Percent_Identity=27.1966527196653, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648441, Length=136, Percent_Identity=37.5, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648439, Length=136, Percent_Identity=37.5, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 53160; Mature: 53160

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILKLSIAEMHDNLKKREFSAVELTKLHIEAVNNEKLNAFITKTPEIALSAAEKADYI
CCCHHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCCEEEEEECCCHHHHHHHHCCCEE
FTHQKENLTPLTGIPVGIKDLFCTKHVRTTACSNILKNFTPQYDSTVTKRLLDNGAVMLG
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEE
KLNMDEFAMGSSNSNSCFGHVKNPWVRADGVEVVPGGSSGGSSAAVAGFLCAGALGSDTG
ECCHHHHCCCCCCCCCEEECCCCCEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
GSVRQPAALCGIVGLKPTYGRCSRFGMIAFASSLDQAGVLTRTVEDASLMLQSICGYDIQ
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DSTSANIAVPKFSESITHTIKGKRIGIPKEYELSGKYQEYTEVSEMWAKGIQYLKDEGAE
CCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
IIEISLPHTSYALPVYYIICSAEASSNLARYDGIRYGARISSDDINEMYELTRGHNFGTE
EEEEECCCCCCCCEEEEEEEECCCCCCHHHHCCCCCCCEECCCHHHHHHHHHCCCCCCHH
VKRRILIGAYVLSSGYYDAYYNKAQRIRHLVINDFVESFKKIDYILTPTTPKEAFAINEQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHEEHHHH
LDTLTMYLNDVFTVPASLAGLPAISVPIGLSKSNLPLSLQVIGNYYDEGGILNVASIIEK
HHHHHHHHHHHHHCCHHHCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHH
HTGKILK
HCCCCCC
>Mature Secondary Structure
MKNILKLSIAEMHDNLKKREFSAVELTKLHIEAVNNEKLNAFITKTPEIALSAAEKADYI
CCCHHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCCEEEEEECCCHHHHHHHHCCCEE
FTHQKENLTPLTGIPVGIKDLFCTKHVRTTACSNILKNFTPQYDSTVTKRLLDNGAVMLG
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEE
KLNMDEFAMGSSNSNSCFGHVKNPWVRADGVEVVPGGSSGGSSAAVAGFLCAGALGSDTG
ECCHHHHCCCCCCCCCEEECCCCCEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
GSVRQPAALCGIVGLKPTYGRCSRFGMIAFASSLDQAGVLTRTVEDASLMLQSICGYDIQ
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DSTSANIAVPKFSESITHTIKGKRIGIPKEYELSGKYQEYTEVSEMWAKGIQYLKDEGAE
CCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
IIEISLPHTSYALPVYYIICSAEASSNLARYDGIRYGARISSDDINEMYELTRGHNFGTE
EEEEECCCCCCCCEEEEEEEECCCCCCHHHHCCCCCCCEECCCHHHHHHHHHCCCCCCHH
VKRRILIGAYVLSSGYYDAYYNKAQRIRHLVINDFVESFKKIDYILTPTTPKEAFAINEQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHEEHHHH
LDTLTMYLNDVFTVPASLAGLPAISVPIGLSKSNLPLSLQVIGNYYDEGGILNVASIIEK
HHHHHHHHHHHHHCCHHHCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHH
HTGKILK
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA