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The map label for this gene is polA [H]

Identifier: 88607943

GI number: 88607943

Start: 122

End: 2683

Strand: Direct

Name: polA [H]

Synonym: APH_0001

Alternate gene names: 88607943

Gene position: 122-2683 (Clockwise)

Preceding gene: NA

Following gene: 88607073

Centisome position: 0.01

GC content: 40.36

Gene sequence:

>2562_bases
GTGATGGAAAAGAAAAGTTTTGTTATCGTCGACGCATATGGGTTTCTTTTTCGCGCGTATTATGCGCTGCCTGGATTAAG
CACCTCATACAATTTTCCTGTAGGAGGTGTATATGGTTTTATAAACATACTTTTGAAACATCTCTCTTTCCACGATGCAG
ATTATTTAGTTGTGGTATTTGATTCGGGGTCGAAAAATTTTCGTCACACTATGTATTCCGAATACAAAACTAATCGCCCT
AAAGCACCAGAGGATCTGTCACTACAATGTGCTCCGCTACGTGAGGCTGTTGAAGCGTTTAATATTGTAAGTGAAGAAGT
GCTTAACTACGAAGCAGACGACGTAATAGCTACACTCTGTACAAAATATGCATCTAGTAATGTTGGAGTGAGAATACTGT
CAGCAGATAAGGATTTACTACAACTCCTAAATGATAATGTTCAAGTTTACGACCCTATAAAAAGCAGATACCTCACCAAT
GAATACGTTTTAGAAAAATTTGGTGTTTCATCAGATAAGTTGCTTGATGCCATGGCTCTTACAGGAGATGCATCGGATAA
TATTCCTGGCGTGCCTAGCATAGGAGTAAAAACTGCTGCAAAGCTGATTAACGAGTTTGGATCACTAGACAATGTGCTGA
ATTCTGTAGAACAGGTGGCTCAGAAAAAGTGTCGCGACATGCTAACCGAGTACGCTGATCGAGCAATTTTATCACGTGAC
TTGATATCTCTGTGTCATGATGTAGAAGTCGAGGGTGATTTGGAGAAGTATGCTAAAAGAGTGCCAGATCCGGTAAAGCT
TATGGCCTTTTTAGAAAAGTATGAACTAACTTCTCTAGAAGGCAAAGTGGCAAAGTACTGCAAGATGGTTGCTGCACCCA
TCCAGAGCAGTGAAGTAGTCAAGAAAGAAAACGAAAAATGCATAGACAAGTTGGAGGAGTTCCTGGAGCAATGTCAGCGC
AGTGGGCTATTAGGGTTATACATAGAAGTAGAGCAAGGTTTTATCAGTACAATATTTGCTGCGTGCGATGAGACTAGATC
TTTTACTGTCAGCAGTCCTGAAAAGGTGCAAAAGTTTGTGGCGGCAGTAACACCAATTCTGCATTCAGATTCGGTGCTCA
AAGTTGTTTATGATGCCAAATCCATGATGCAGTTTTTTCCTGAGTTGCGGGCATTTGACGATATTATGATTATGTCGTAC
AGCCTTGATACAGGTCATCACGATCACGGCTTTGATAGCATGTCGCAGCGTCATCTTGGTCAAATATTTGGTATTACCAA
TGGGTGCATATTGATACGGTTGCATCGAGTTATAATGAGAAAATTATTCTCAGCTAAGCTGTACACCGTTTATTACACAC
TCGAAAGGCCGTTAGTACCTGTAATATACGAATTGGAAAAAACAGGGATAAAAATAGACACTGAGTTATTGAAGCAATTA
TCTGATGAGTTTTCTCTATCCATAAGTAATTTGGAAGAAGAAATATTCGGCCTTGCAGGATGTAAATTTAACATAGCTTC
CTCACAGCAGTTGGGTAAAGTCCTCTTTGAGCATATGCGTATTGGGGGAGCTAAAAAAATGCCTTCTGGCAGCTACTGCA
CCAATGCGGAAGTTCTTACTGCACTGGCATTAGATGGAGTTGAAGTTGCTGATAAAATTCTGAAGTGGCGTCACTTTACT
AAATTAAAGAGCACGTACACAGACGCTTTAGTCCGGCAGGTTGATGCTAATACTGGAAGGGTGCATACAAAATATTCCAT
GACTGCTACGGCGACAGGACGGCTCAGTTCAAGTAACCCAAATTTGCAGAATATACCTATCAGGAGCAAAGATGGGGAAA
AAATTCGCAGTGCATTTATTGCTGAGAAAGGCCATAAATTGATAGCAGCAGATTATTCACAAATGGAACTCAAAATCTTG
GCACATATTGCTGATGTAAAAGCTTTTAGACAGGCTTTTGCTGAGGGATTAGACATTCACGTCGTTACAGCACAGCAAAT
TTTTGGTGAGTATTATGAAATTGATTATGAGCTCAGAAGAAGGGCAAAATCTATCAATTTCGGGATAATCTATGGTATGG
GATCTTATGGATTAGCGAGAAATATAGGCATAAGCAGAAGGGAAGCTTCTGAGTACGTGGAACAGTATTTTCAATACTAT
CCTGAAATCAAGCGTTATATGGAAACAACAAAGGCATATGCAAAAAAGCATGGATATACTATTACTGCTTTTGGGAGGAA
GTGCTTTATTGAAGGGATTAATAGTCCAAAGCGAGCTTTAAGTAGTGTTGCAGAACGAGCTGCAATTAACGCACCAATCC
AGGGTACTGCAGCTGATATTGTAAAAAAGGCTATGGTAAGGTTGCAAGATCGACTCACGCAGGGAACAATGCTTCTCCAA
GTCCATGACGAATTATTGGTAGAAGTCCCAGAAGAGCACGTACTCTCAGTTGCACGTTTGATGAAAGAAGTAATGGAGAG
TGCTGTGGTGTTTTCTCTACCTTTGGATGTTAATATAAGCGTTGGAGATAATTGGGGAGACATGGTTGAAATCGAGGTAT
AA

Upstream 100 bases:

>100_bases
ACCTACAGCGGAATTTTTTCTCAATGTTGTAAGTAATAATTCTTCGTACTAGAGAAGTTTTGAGTTTTACCATATCCTAG
GGTTTTAGTCATAAAACAGT

Downstream 100 bases:

>100_bases
GGATGTATCACGAAGTGCATGCGATTTTAAGATTTATACTTTTAATCGATAAGTTAAATGCGTATGCACAATTTTGGCGT
TTTACCTGTTGCAGCTTGAA

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 853; Mature: 853

Protein sequence:

>853_residues
MMEKKSFVIVDAYGFLFRAYYALPGLSTSYNFPVGGVYGFINILLKHLSFHDADYLVVVFDSGSKNFRHTMYSEYKTNRP
KAPEDLSLQCAPLREAVEAFNIVSEEVLNYEADDVIATLCTKYASSNVGVRILSADKDLLQLLNDNVQVYDPIKSRYLTN
EYVLEKFGVSSDKLLDAMALTGDASDNIPGVPSIGVKTAAKLINEFGSLDNVLNSVEQVAQKKCRDMLTEYADRAILSRD
LISLCHDVEVEGDLEKYAKRVPDPVKLMAFLEKYELTSLEGKVAKYCKMVAAPIQSSEVVKKENEKCIDKLEEFLEQCQR
SGLLGLYIEVEQGFISTIFAACDETRSFTVSSPEKVQKFVAAVTPILHSDSVLKVVYDAKSMMQFFPELRAFDDIMIMSY
SLDTGHHDHGFDSMSQRHLGQIFGITNGCILIRLHRVIMRKLFSAKLYTVYYTLERPLVPVIYELEKTGIKIDTELLKQL
SDEFSLSISNLEEEIFGLAGCKFNIASSQQLGKVLFEHMRIGGAKKMPSGSYCTNAEVLTALALDGVEVADKILKWRHFT
KLKSTYTDALVRQVDANTGRVHTKYSMTATATGRLSSSNPNLQNIPIRSKDGEKIRSAFIAEKGHKLIAADYSQMELKIL
AHIADVKAFRQAFAEGLDIHVVTAQQIFGEYYEIDYELRRRAKSINFGIIYGMGSYGLARNIGISRREASEYVEQYFQYY
PEIKRYMETTKAYAKKHGYTITAFGRKCFIEGINSPKRALSSVAERAAINAPIQGTAADIVKKAMVRLQDRLTQGTMLLQ
VHDELLVEVPEEHVLSVARLMKEVMESAVVFSLPLDVNISVGDNWGDMVEIEV

Sequences:

>Translated_853_residues
MMEKKSFVIVDAYGFLFRAYYALPGLSTSYNFPVGGVYGFINILLKHLSFHDADYLVVVFDSGSKNFRHTMYSEYKTNRP
KAPEDLSLQCAPLREAVEAFNIVSEEVLNYEADDVIATLCTKYASSNVGVRILSADKDLLQLLNDNVQVYDPIKSRYLTN
EYVLEKFGVSSDKLLDAMALTGDASDNIPGVPSIGVKTAAKLINEFGSLDNVLNSVEQVAQKKCRDMLTEYADRAILSRD
LISLCHDVEVEGDLEKYAKRVPDPVKLMAFLEKYELTSLEGKVAKYCKMVAAPIQSSEVVKKENEKCIDKLEEFLEQCQR
SGLLGLYIEVEQGFISTIFAACDETRSFTVSSPEKVQKFVAAVTPILHSDSVLKVVYDAKSMMQFFPELRAFDDIMIMSY
SLDTGHHDHGFDSMSQRHLGQIFGITNGCILIRLHRVIMRKLFSAKLYTVYYTLERPLVPVIYELEKTGIKIDTELLKQL
SDEFSLSISNLEEEIFGLAGCKFNIASSQQLGKVLFEHMRIGGAKKMPSGSYCTNAEVLTALALDGVEVADKILKWRHFT
KLKSTYTDALVRQVDANTGRVHTKYSMTATATGRLSSSNPNLQNIPIRSKDGEKIRSAFIAEKGHKLIAADYSQMELKIL
AHIADVKAFRQAFAEGLDIHVVTAQQIFGEYYEIDYELRRRAKSINFGIIYGMGSYGLARNIGISRREASEYVEQYFQYY
PEIKRYMETTKAYAKKHGYTITAFGRKCFIEGINSPKRALSSVAERAAINAPIQGTAADIVKKAMVRLQDRLTQGTMLLQ
VHDELLVEVPEEHVLSVARLMKEVMESAVVFSLPLDVNISVGDNWGDMVEIEV
>Mature_853_residues
MMEKKSFVIVDAYGFLFRAYYALPGLSTSYNFPVGGVYGFINILLKHLSFHDADYLVVVFDSGSKNFRHTMYSEYKTNRP
KAPEDLSLQCAPLREAVEAFNIVSEEVLNYEADDVIATLCTKYASSNVGVRILSADKDLLQLLNDNVQVYDPIKSRYLTN
EYVLEKFGVSSDKLLDAMALTGDASDNIPGVPSIGVKTAAKLINEFGSLDNVLNSVEQVAQKKCRDMLTEYADRAILSRD
LISLCHDVEVEGDLEKYAKRVPDPVKLMAFLEKYELTSLEGKVAKYCKMVAAPIQSSEVVKKENEKCIDKLEEFLEQCQR
SGLLGLYIEVEQGFISTIFAACDETRSFTVSSPEKVQKFVAAVTPILHSDSVLKVVYDAKSMMQFFPELRAFDDIMIMSY
SLDTGHHDHGFDSMSQRHLGQIFGITNGCILIRLHRVIMRKLFSAKLYTVYYTLERPLVPVIYELEKTGIKIDTELLKQL
SDEFSLSISNLEEEIFGLAGCKFNIASSQQLGKVLFEHMRIGGAKKMPSGSYCTNAEVLTALALDGVEVADKILKWRHFT
KLKSTYTDALVRQVDANTGRVHTKYSMTATATGRLSSSNPNLQNIPIRSKDGEKIRSAFIAEKGHKLIAADYSQMELKIL
AHIADVKAFRQAFAEGLDIHVVTAQQIFGEYYEIDYELRRRAKSINFGIIYGMGSYGLARNIGISRREASEYVEQYFQYY
PEIKRYMETTKAYAKKHGYTITAFGRKCFIEGINSPKRALSSVAERAAINAPIQGTAADIVKKAMVRLQDRLTQGTMLLQ
VHDELLVEVPEEHVLSVARLMKEVMESAVVFSLPLDVNISVGDNWGDMVEIEV

Specific function: In addition to polymerase activity, this DNA polymerase 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=463, Percent_Identity=29.8056155507559, Blast_Score=204, Evalue=4e-52,
Organism=Homo sapiens, GI139394648, Length=530, Percent_Identity=27.9245283018868, Blast_Score=166, Evalue=7e-41,
Organism=Escherichia coli, GI1790294, Length=927, Percent_Identity=35.814455231931, Blast_Score=534, Evalue=1e-152,
Organism=Escherichia coli, GI226510970, Length=204, Percent_Identity=33.8235294117647, Blast_Score=121, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI86563272, Length=397, Percent_Identity=31.9899244332494, Blast_Score=182, Evalue=9e-46,
Organism=Drosophila melanogaster, GI17933644, Length=472, Percent_Identity=29.0254237288136, Blast_Score=203, Evalue=5e-52,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 95778; Mature: 95778

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEKKSFVIVDAYGFLFRAYYALPGLSTSYNFPVGGVYGFINILLKHLSFHDADYLVVVF
CCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
DSGSKNFRHTMYSEYKTNRPKAPEDLSLQCAPLREAVEAFNIVSEEVLNYEADDVIATLC
ECCCCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TKYASSNVGVRILSADKDLLQLLNDNVQVYDPIKSRYLTNEYVLEKFGVSSDKLLDAMAL
HHHHCCCCCEEEEECCHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TGDASDNIPGVPSIGVKTAAKLINEFGSLDNVLNSVEQVAQKKCRDMLTEYADRAILSRD
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LISLCHDVEVEGDLEKYAKRVPDPVKLMAFLEKYELTSLEGKVAKYCKMVAAPIQSSEVV
HHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
KKENEKCIDKLEEFLEQCQRSGLLGLYIEVEQGFISTIFAACDETRSFTVSSPEKVQKFV
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AAVTPILHSDSVLKVVYDAKSMMQFFPELRAFDDIMIMSYSLDTGHHDHGFDSMSQRHLG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHH
QIFGITNGCILIRLHRVIMRKLFSAKLYTVYYTLERPLVPVIYELEKTGIKIDTELLKQL
HHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCEECHHHHHHH
SDEFSLSISNLEEEIFGLAGCKFNIASSQQLGKVLFEHMRIGGAKKMPSGSYCTNAEVLT
HHHHHEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
ALALDGVEVADKILKWRHFTKLKSTYTDALVRQVDANTGRVHTKYSMTATATGRLSSSNP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCC
NLQNIPIRSKDGEKIRSAFIAEKGHKLIAADYSQMELKILAHIADVKAFRQAFAEGLDIH
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VVTAQQIFGEYYEIDYELRRRAKSINFGIIYGMGSYGLARNIGISRREASEYVEQYFQYY
EEEHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHH
PEIKRYMETTKAYAKKHGYTITAFGRKCFIEGINSPKRALSSVAERAAINAPIQGTAADI
HHHHHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
VKKAMVRLQDRLTQGTMLLQVHDELLVEVPEEHVLSVARLMKEVMESAVVFSLPLDVNIS
HHHHHHHHHHHHHCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEECCEEEEE
VGDNWGDMVEIEV
ECCCCCCEEEEEC
>Mature Secondary Structure
MMEKKSFVIVDAYGFLFRAYYALPGLSTSYNFPVGGVYGFINILLKHLSFHDADYLVVVF
CCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
DSGSKNFRHTMYSEYKTNRPKAPEDLSLQCAPLREAVEAFNIVSEEVLNYEADDVIATLC
ECCCCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TKYASSNVGVRILSADKDLLQLLNDNVQVYDPIKSRYLTNEYVLEKFGVSSDKLLDAMAL
HHHHCCCCCEEEEECCHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TGDASDNIPGVPSIGVKTAAKLINEFGSLDNVLNSVEQVAQKKCRDMLTEYADRAILSRD
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LISLCHDVEVEGDLEKYAKRVPDPVKLMAFLEKYELTSLEGKVAKYCKMVAAPIQSSEVV
HHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
KKENEKCIDKLEEFLEQCQRSGLLGLYIEVEQGFISTIFAACDETRSFTVSSPEKVQKFV
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AAVTPILHSDSVLKVVYDAKSMMQFFPELRAFDDIMIMSYSLDTGHHDHGFDSMSQRHLG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHH
QIFGITNGCILIRLHRVIMRKLFSAKLYTVYYTLERPLVPVIYELEKTGIKIDTELLKQL
HHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCEECHHHHHHH
SDEFSLSISNLEEEIFGLAGCKFNIASSQQLGKVLFEHMRIGGAKKMPSGSYCTNAEVLT
HHHHHEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
ALALDGVEVADKILKWRHFTKLKSTYTDALVRQVDANTGRVHTKYSMTATATGRLSSSNP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCC
NLQNIPIRSKDGEKIRSAFIAEKGHKLIAADYSQMELKILAHIADVKAFRQAFAEGLDIH
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VVTAQQIFGEYYEIDYELRRRAKSINFGIIYGMGSYGLARNIGISRREASEYVEQYFQYY
EEEHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHH
PEIKRYMETTKAYAKKHGYTITAFGRKCFIEGINSPKRALSSVAERAAINAPIQGTAADI
HHHHHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
VKKAMVRLQDRLTQGTMLLQVHDELLVEVPEEHVLSVARLMKEVMESAVVFSLPLDVNIS
HHHHHHHHHHHHHCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEECCEEEEE
VGDNWGDMVEIEV
ECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA