Click here to switch to the map view.

The map label for this gene is metK [H]

Identifier: 88607858

GI number: 88607858

Start: 66297

End: 67322

Strand: Direct

Name: metK [H]

Synonym: APH_0058

Alternate gene names: 88607858

Gene position: 66297-67322 (Clockwise)

Preceding gene: 88606852

Following gene: 88606901

Centisome position: 4.51

GC content: 41.52

Gene sequence:

>1026_bases
ATGCCACTAAGTGAAGCAGCAACTATAGCAGGAGAAGTAAGCGGAGTAATAGTAGAGGAGGAAAAGGTTGACGGAGTAGT
TCGTAAGACACTGAGAGAAATAGGATACGAAAAGGAAGGATTTCACTGGAGAGAGGTTAAAGTGAGGATCATGCTCCAGG
AACAATCTCATGATATAATGTTGGGAGTGAATCGTGGTCGTAAAAAGAAGGGAGCAGGAGATCAGGGAGTAATGTATGGC
TATGCCATAAACGAGACAGAATCATACATGCCGTCTCCAATATTTTACTCCCACCGTGTTCTTAAAAATTTGTCAGATGC
AGTGAAACAAGGAAGCATTACCGGTATAGGACCAGATGCAAAGACAGAAATAACCCTCCAGTATGTTGACCATAAGCCCG
TAAAAGTGTTGAGAGCAGTACTTTCAGTGCAGCATGATGAAAGTAGAACACAACAAGACGTCAGGGATCTTGTCTATCCA
TACTTCGTATCATCGATACCAGAAGGATGGATGTGTAAAGAAGAGAATTTTCTAGTCAATCCTACAGGAAGGTTCGTTGT
AGGAGGGCCAGTGGGTGATACAGGAATCACTGGTAGAAAGATAATGGTTGATACCTACGGAGGCCACATACCGCACGGCG
GAGGCGCTTTTTCAGGAAAGGATCCAAGTAAGGTAGATCGTTCCGCAGCGTATATGGCAAGATACATAGCTAAAAATGTA
GTACATGCAGGTCTAGCTAGTGAATGTTTGGTACAGATGTCATATGCAATAGGTGTACCAGCTCCACTGACTTTTGCAAT
CAATACGTTTGGAACCAGCGTGGTTGATGTAAAGGAGATAGAAAGGTGTATTATAAGTAATATTGATCTTTCAGTCTCAG
GAATATGTGAAAAGCTGTCGCTATATGAACAAATATATAGCCCTACGTCATGTTATGGACATTTTGGAAGGACTCATGAG
AATAGTTTTTCATGGGAAAAGCTAGACCTGTCTCTAATTCTCAATAGAGAGTTTTCTTGTAATTAA

Upstream 100 bases:

>100_bases
TATCAGCCATATAGCCATCGGTAGCGCAAAAAACATGTTCCTCACCGCACCTTGATATTACGAGTCTATATCTTCTCCAA
GCCCCACTTACACTTTTGTC

Downstream 100 bases:

>100_bases
TGTAATATAACTACCCACAGTAAAATAAGCCGATTCACAGGGCTTTTTGTTGTGACGGGATTAAGTATAAAAATAACTAA
ACAATCAGTAGGATATTGAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MPLSEAATIAGEVSGVIVEEEKVDGVVRKTLREIGYEKEGFHWREVKVRIMLQEQSHDIMLGVNRGRKKKGAGDQGVMYG
YAINETESYMPSPIFYSHRVLKNLSDAVKQGSITGIGPDAKTEITLQYVDHKPVKVLRAVLSVQHDESRTQQDVRDLVYP
YFVSSIPEGWMCKEENFLVNPTGRFVVGGPVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNV
VHAGLASECLVQMSYAIGVPAPLTFAINTFGTSVVDVKEIERCIISNIDLSVSGICEKLSLYEQIYSPTSCYGHFGRTHE
NSFSWEKLDLSLILNREFSCN

Sequences:

>Translated_341_residues
MPLSEAATIAGEVSGVIVEEEKVDGVVRKTLREIGYEKEGFHWREVKVRIMLQEQSHDIMLGVNRGRKKKGAGDQGVMYG
YAINETESYMPSPIFYSHRVLKNLSDAVKQGSITGIGPDAKTEITLQYVDHKPVKVLRAVLSVQHDESRTQQDVRDLVYP
YFVSSIPEGWMCKEENFLVNPTGRFVVGGPVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNV
VHAGLASECLVQMSYAIGVPAPLTFAINTFGTSVVDVKEIERCIISNIDLSVSGICEKLSLYEQIYSPTSCYGHFGRTHE
NSFSWEKLDLSLILNREFSCN
>Mature_340_residues
PLSEAATIAGEVSGVIVEEEKVDGVVRKTLREIGYEKEGFHWREVKVRIMLQEQSHDIMLGVNRGRKKKGAGDQGVMYGY
AINETESYMPSPIFYSHRVLKNLSDAVKQGSITGIGPDAKTEITLQYVDHKPVKVLRAVLSVQHDESRTQQDVRDLVYPY
FVSSIPEGWMCKEENFLVNPTGRFVVGGPVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNVV
HAGLASECLVQMSYAIGVPAPLTFAINTFGTSVVDVKEIERCIISNIDLSVSGICEKLSLYEQIYSPTSCYGHFGRTHEN
SFSWEKLDLSLILNREFSCN

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=328, Percent_Identity=42.6829268292683, Blast_Score=244, Evalue=7e-65,
Organism=Homo sapiens, GI5174529, Length=328, Percent_Identity=43.5975609756098, Blast_Score=244, Evalue=7e-65,
Organism=Escherichia coli, GI1789311, Length=316, Percent_Identity=47.7848101265823, Blast_Score=298, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17538498, Length=328, Percent_Identity=41.4634146341463, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17538494, Length=328, Percent_Identity=41.4634146341463, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=41.4634146341463, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI25145633, Length=328, Percent_Identity=42.3780487804878, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI25145635, Length=328, Percent_Identity=42.3780487804878, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI32565905, Length=295, Percent_Identity=43.0508474576271, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI32565903, Length=297, Percent_Identity=42.7609427609428, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17551082, Length=328, Percent_Identity=39.9390243902439, Blast_Score=228, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6323209, Length=326, Percent_Identity=40.7975460122699, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6320710, Length=326, Percent_Identity=40.7975460122699, Blast_Score=233, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24580541, Length=329, Percent_Identity=42.8571428571429, Blast_Score=231, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24580527, Length=329, Percent_Identity=42.8571428571429, Blast_Score=231, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24580525, Length=329, Percent_Identity=42.8571428571429, Blast_Score=231, Evalue=5e-61,
Organism=Drosophila melanogaster, GI45552159, Length=329, Percent_Identity=43.161094224924, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24580529, Length=329, Percent_Identity=43.161094224924, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24580537, Length=329, Percent_Identity=43.161094224924, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24580533, Length=329, Percent_Identity=43.161094224924, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24580535, Length=329, Percent_Identity=43.161094224924, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24580531, Length=329, Percent_Identity=43.161094224924, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 37679; Mature: 37548

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSEAATIAGEVSGVIVEEEKVDGVVRKTLREIGYEKEGFHWREVKVRIMLQEQSHDIM
CCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCEE
LGVNRGRKKKGAGDQGVMYGYAINETESYMPSPIFYSHRVLKNLSDAVKQGSITGIGPDA
EEECCCCCCCCCCCCCEEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCC
KTEITLQYVDHKPVKVLRAVLSVQHDESRTQQDVRDLVYPYFVSSIPEGWMCKEENFLVN
CCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEC
PTGRFVVGGPVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNV
CCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VHAGLASECLVQMSYAIGVPAPLTFAINTFGTSVVDVKEIERCIISNIDLSVSGICEKLS
HHHCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHCCCCCHHHHHHHHH
LYEQIYSPTSCYGHFGRTHENSFSWEKLDLSLILNREFSCN
HHHHHCCCHHHHHCCCCCCCCCCCHHEEEEEEEECCCCCCC
>Mature Secondary Structure 
PLSEAATIAGEVSGVIVEEEKVDGVVRKTLREIGYEKEGFHWREVKVRIMLQEQSHDIM
CCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCEE
LGVNRGRKKKGAGDQGVMYGYAINETESYMPSPIFYSHRVLKNLSDAVKQGSITGIGPDA
EEECCCCCCCCCCCCCEEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCC
KTEITLQYVDHKPVKVLRAVLSVQHDESRTQQDVRDLVYPYFVSSIPEGWMCKEENFLVN
CCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEC
PTGRFVVGGPVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNV
CCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VHAGLASECLVQMSYAIGVPAPLTFAINTFGTSVVDVKEIERCIISNIDLSVSGICEKLS
HHHCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHCCCCCHHHHHHHHH
LYEQIYSPTSCYGHFGRTHENSFSWEKLDLSLILNREFSCN
HHHHHCCCHHHHHCCCCCCCCCCCHHEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA