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The map label for this gene is tig

Identifier: 88607838

GI number: 88607838

Start: 1032081

End: 1033400

Strand: Reverse

Name: tig

Synonym: APH_0971

Alternate gene names: 88607838

Gene position: 1033400-1032081 (Counterclockwise)

Preceding gene: 88607278

Following gene: 88607298

Centisome position: 70.24

GC content: 45.38

Gene sequence:

>1320_bases
ATGCAGCAGTTCTATTGTGTCAAAGAGGTTCTCAACGAGCGGCTTAAGCGTGGGTATGAGTTTGCTGTCAAAAACGAGTA
CATAGAAGAGAAGGTAACTGAGAAGCTTCAGGGGATTGCGGCTACAGCACGTATGGATGGTTTTAGGAAGGGTAAGGTTT
CTCCGGAGTTTGTGCGCAGAATGTATGGAGAGAGTATAGCGGGCGAAGTAGTAGCGCAGGTTATTGATGATATTTCTTCT
AAGTTTCTGAAGGAGGGTGACTTCGGAGGTATAGTGACTTCAGGTGTGGAAATCAAGGCTTATCCCAAGGTTTGTTCGAA
TAGTGAGGGCGATGGGGGTTTGGTTTATGAGCTGAAGTTTGAGGTAATGCCGGAGGTTCCAGTTGTTGATATTGATGGTA
TTACTCTGAAGGAGATTGAGGTAAATATCTCGCAAGAGGATGTTGATGAGTTTCTAGAAGATCTTAAGGCAAACTACCCG
AGCTTTGTTGATGTTGATGATGAATCTCGTGGGGTTTCAGATGGTGATCGGGTGACGATAAGTTATCGTAGCGCGTTTAA
GGGAAAGGCGCTGAGAGGAGGAAGTGCGCAGGGGTTTACATTTGTTTTGGGAAAGGGGCAATTGCTAGAGCAGTTTGAGA
GGCAAATTGTTGGAATGAAGAAGGGTGAATCTAAGGAATTTAAGTTGGAGTTCCCTCTCGATTATATGGCTAAGCACTTT
GCTGGAAAGGAAGTAGACATGCATGTGACGGTAGAGAGACTTAGCGTAAAGGATGAGATAAATGACAGGGAGACATTGGC
GAGCAAGTGTGGCTTTGGGAGCGTAGAAGATATGGTGAAGTTTGCCACGGATGGTCTCAATCGTCGTTTTGCGGATATGG
GTAGTGTAGTGGCTCAGAGAGAGCTGCTTGAACATTTGGATGCGTCGTACAGTGTGGATGTTCCTGAATATATAGTTGCT
CAAGAGCTTGGAAGGATGCGTCGTGAGCTAGAAGAAGGGGATTTGAATGATGCTGAGGCTCTGACTAAGGAAGCTGAGCA
GCGTGTAAAGCTTGGTATGTTGCTCATGAAGGTGGCAACTGATGCTGGGGTTGCTGTGGAGGTCAATGATATTTTGTCCT
TTATCAGGGTGAATTACTCTGCCTACGGAAGGAGCATGGAAGATGTGCTTAAGCTTTTTAAGAACAGAGAGGATTTTAGG
GAACACGTGAAGGGAAAAGTCCTTGAAGATAAGGTGGTCAGGTATATAATTTCCAGGGCTAAAAAGGATAGGCAAAGCAT
GTCTGCGGGGGAGCTTAAATCGCTTTTTGAGAGTGTGTAG

Upstream 100 bases:

>100_bases
GTAGACGTGCTAGGTTTAGGTCCTAGTGAGTTATGCTCGTGGGGGTTCAAGTCCCTCTATCCGCACTCCTGTGTGCTTGG
AGTGTAAGAGGAGTGTTTTT

Downstream 100 bases:

>100_bases
GTAATAGTTTTAAGCTTGTCTTGAGGGTTTGTGTATGGGATTTGGGGGGTAGTCTATGAACTTGGTTCCGATGGTAGTTG
AGCAGACTGGTAGGGGTGAG

Product: putative trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MQQFYCVKEVLNERLKRGYEFAVKNEYIEEKVTEKLQGIAATARMDGFRKGKVSPEFVRRMYGESIAGEVVAQVIDDISS
KFLKEGDFGGIVTSGVEIKAYPKVCSNSEGDGGLVYELKFEVMPEVPVVDIDGITLKEIEVNISQEDVDEFLEDLKANYP
SFVDVDDESRGVSDGDRVTISYRSAFKGKALRGGSAQGFTFVLGKGQLLEQFERQIVGMKKGESKEFKLEFPLDYMAKHF
AGKEVDMHVTVERLSVKDEINDRETLASKCGFGSVEDMVKFATDGLNRRFADMGSVVAQRELLEHLDASYSVDVPEYIVA
QELGRMRRELEEGDLNDAEALTKEAEQRVKLGMLLMKVATDAGVAVEVNDILSFIRVNYSAYGRSMEDVLKLFKNREDFR
EHVKGKVLEDKVVRYIISRAKKDRQSMSAGELKSLFESV

Sequences:

>Translated_439_residues
MQQFYCVKEVLNERLKRGYEFAVKNEYIEEKVTEKLQGIAATARMDGFRKGKVSPEFVRRMYGESIAGEVVAQVIDDISS
KFLKEGDFGGIVTSGVEIKAYPKVCSNSEGDGGLVYELKFEVMPEVPVVDIDGITLKEIEVNISQEDVDEFLEDLKANYP
SFVDVDDESRGVSDGDRVTISYRSAFKGKALRGGSAQGFTFVLGKGQLLEQFERQIVGMKKGESKEFKLEFPLDYMAKHF
AGKEVDMHVTVERLSVKDEINDRETLASKCGFGSVEDMVKFATDGLNRRFADMGSVVAQRELLEHLDASYSVDVPEYIVA
QELGRMRRELEEGDLNDAEALTKEAEQRVKLGMLLMKVATDAGVAVEVNDILSFIRVNYSAYGRSMEDVLKLFKNREDFR
EHVKGKVLEDKVVRYIISRAKKDRQSMSAGELKSLFESV
>Mature_439_residues
MQQFYCVKEVLNERLKRGYEFAVKNEYIEEKVTEKLQGIAATARMDGFRKGKVSPEFVRRMYGESIAGEVVAQVIDDISS
KFLKEGDFGGIVTSGVEIKAYPKVCSNSEGDGGLVYELKFEVMPEVPVVDIDGITLKEIEVNISQEDVDEFLEDLKANYP
SFVDVDDESRGVSDGDRVTISYRSAFKGKALRGGSAQGFTFVLGKGQLLEQFERQIVGMKKGESKEFKLEFPLDYMAKHF
AGKEVDMHVTVERLSVKDEINDRETLASKCGFGSVEDMVKFATDGLNRRFADMGSVVAQRELLEHLDASYSVDVPEYIVA
QELGRMRRELEEGDLNDAEALTKEAEQRVKLGMLLMKVATDAGVAVEVNDILSFIRVNYSAYGRSMEDVLKLFKNREDFR
EHVKGKVLEDKVVRYIISRAKKDRQSMSAGELKSLFESV

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=429, Percent_Identity=24.4755244755245, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_ANAPZ (Q2GJB3)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505538.1
- ProteinModelPortal:   Q2GJB3
- SMR:   Q2GJB3
- STRING:   Q2GJB3
- GeneID:   3930667
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0971
- TIGR:   APH_0971
- eggNOG:   COG0544
- HOGENOM:   HBG311097
- OMA:   FEGRIRN
- ProtClustDB:   CLSK749502
- BioCyc:   APHA212042:APH_0971-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49390; Mature: 49390

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQFYCVKEVLNERLKRGYEFAVKNEYIEEKVTEKLQGIAATARMDGFRKGKVSPEFVRR
CCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
MYGESIAGEVVAQVIDDISSKFLKEGDFGGIVTSGVEIKAYPKVCSNSEGDGGLVYELKF
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCHHHCCCCCCCCEEEEEEE
EVMPEVPVVDIDGITLKEIEVNISQEDVDEFLEDLKANYPSFVDVDDESRGVSDGDRVTI
EECCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEE
SYRSAFKGKALRGGSAQGFTFVLGKGQLLEQFERQIVGMKKGESKEFKLEFPLDYMAKHF
EECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHH
AGKEVDMHVTVERLSVKDEINDRETLASKCGFGSVEDMVKFATDGLNRRFADMGSVVAQR
CCCCEEEEEEHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLEHLDASYSVDVPEYIVAQELGRMRRELEEGDLNDAEALTKEAEQRVKLGMLLMKVAT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
DAGVAVEVNDILSFIRVNYSAYGRSMEDVLKLFKNREDFREHVKGKVLEDKVVRYIISRA
CCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHH
KKDRQSMSAGELKSLFESV
HHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MQQFYCVKEVLNERLKRGYEFAVKNEYIEEKVTEKLQGIAATARMDGFRKGKVSPEFVRR
CCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
MYGESIAGEVVAQVIDDISSKFLKEGDFGGIVTSGVEIKAYPKVCSNSEGDGGLVYELKF
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCHHHCCCCCCCCEEEEEEE
EVMPEVPVVDIDGITLKEIEVNISQEDVDEFLEDLKANYPSFVDVDDESRGVSDGDRVTI
EECCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEE
SYRSAFKGKALRGGSAQGFTFVLGKGQLLEQFERQIVGMKKGESKEFKLEFPLDYMAKHF
EECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHH
AGKEVDMHVTVERLSVKDEINDRETLASKCGFGSVEDMVKFATDGLNRRFADMGSVVAQR
CCCCEEEEEEHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLEHLDASYSVDVPEYIVAQELGRMRRELEEGDLNDAEALTKEAEQRVKLGMLLMKVAT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
DAGVAVEVNDILSFIRVNYSAYGRSMEDVLKLFKNREDFREHVKGKVLEDKVVRYIISRA
CCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHH
KKDRQSMSAGELKSLFESV
HHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA