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The map label for this gene is glnG [C]

Identifier: 88607673

GI number: 88607673

Start: 194341

End: 195729

Strand: Direct

Name: glnG [C]

Synonym: APH_0196

Alternate gene names: 88607673

Gene position: 194341-195729 (Clockwise)

Preceding gene: 88607591

Following gene: 88607640

Centisome position: 13.21

GC content: 44.13

Gene sequence:

>1389_bases
ATGTCTAAGGTAAAGAGATTTTACATACCCGAAGTTCTAATCGTGGATGATGAGGCTGATTTAAGAGCTATGATCCAGGA
TATATTGAGGGATGATAACTACGTGACAAGGGTCGCAGCTGATGGGTTGTCAGCCATGAAACTTGCATATGAAAGAGAGC
CAGATGTAGTACTTCTCGATATATGGTTGAAGGGGTCTGACATAGATGGATTAAGCGTATTGGAGAAGCTGAAGGAGCGG
TATCCGAGTCTTCCCGTTATTATGATAAGTGGGCATGGCAATATAGCTACTGCTGTTAAGTCACTGCACATAGGTGCGTA
TGATTACATCGAGAAGCCATTTACGGAAAATAGATTAAAGTTGGTGGTAAAGCGGGCTGTAGAATCCGGAAGGCTGAGAA
GGGAGAATGATGAATTGAGGTCCTTCTTTGAGGATTATGAGCTTATAGGCAATTCTCCTCAGATAAAGAGCCTGAGGTCT
ACTGTTAACAAGGCTGCTTCCACATTTAGTAGGATTTTGATTACTGGTGCTCCGGGGACTGGTAAAGAGGTTGTCGCCAG
GTTAATTCATAAGAAATTCCGTGGTGGGAGTACGTTTGTTAGTTTCTGCCCGTCCATTCTGCCGGAAAATAGTTACTTGG
AGAATATTTTTGGTAGTGAAGGTGAGAATAGCTCGCTGCAGCATGTGGTGCCGCACAGTGTTGGAATTATAGAGCAAGCT
AATCACGGTACTCTGTTTATAGATGAGGTGACTGACCTTCGCTACGATGCGCAGCTTAGATTGATGCGCTTGCTTCAAGA
AGGTAGGATATATAGGGAAAGTGGTAAAATCCCTGTAATTGTTGATACTAGAGTGATTGCATCGTCTTCTAAGATTATGG
AAGATGAGGTGAGGCTTGGAAAATTCTGTGAGGATCTCTACTATAGGTTGAATGTTTTCCCGATACGTGTTCCATCGCTT
TCGGAATACTGTGCTGATCTTCCGGAGATTTGTGAATACATGATGAAAAGCATATGTAAGAAGATGAGGCTTTTCCCGCG
TGCTATTAGTGAGGAAGCGATAGTGGCTATGCAGTCATACTGTTGGCCTGGAAATCTGCGTCAGCTTCGTAATGTCCTTG
AATGGGTCATGATTATGCAGACTAAGCATGATGTGATCACAGTAGATGACTTACCGGCGGAGATAGTCAACGGTGCTCCT
ATCAACAGTGTGTTCACGCATATAGTTTCGGCGCCATTGAGGAAGGCAAGGGAGGAATTCGAGCGACAGTATCTTAAGAC
GCAGTTATCTAGGTTTGGTGGTAGTGTTTCTAGGACAGCAGAGTTTATTGGAATGGAGCGCTCAGCGTTGCATAGGAAGC
TGAAAATGCTGGGGCTGTTTACCGGATAG

Upstream 100 bases:

>100_bases
AAAGTGAAGAATCGATAGGTGCTGTATTGTTTTTACAAGATTAAGCGTATTTTTACTCTGCAGAGTAGAATGTTGGCTAA
TCCAAAAGAGTGTGGGGTTT

Downstream 100 bases:

>100_bases
TGGCGCAGAGAGGAGTAGATATTGGAACTCTGCATACTTAAATAGTTATGTGGTGCAGTCTAAGGCGTTTTTATCGAGTG
TGCTGCGTGCTCTTTATCCG

Product: nitrogen assimilation regulatory protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSKVKRFYIPEVLIVDDEADLRAMIQDILRDDNYVTRVAADGLSAMKLAYEREPDVVLLDIWLKGSDIDGLSVLEKLKER
YPSLPVIMISGHGNIATAVKSLHIGAYDYIEKPFTENRLKLVVKRAVESGRLRRENDELRSFFEDYELIGNSPQIKSLRS
TVNKAASTFSRILITGAPGTGKEVVARLIHKKFRGGSTFVSFCPSILPENSYLENIFGSEGENSSLQHVVPHSVGIIEQA
NHGTLFIDEVTDLRYDAQLRLMRLLQEGRIYRESGKIPVIVDTRVIASSSKIMEDEVRLGKFCEDLYYRLNVFPIRVPSL
SEYCADLPEICEYMMKSICKKMRLFPRAISEEAIVAMQSYCWPGNLRQLRNVLEWVMIMQTKHDVITVDDLPAEIVNGAP
INSVFTHIVSAPLRKAREEFERQYLKTQLSRFGGSVSRTAEFIGMERSALHRKLKMLGLFTG

Sequences:

>Translated_462_residues
MSKVKRFYIPEVLIVDDEADLRAMIQDILRDDNYVTRVAADGLSAMKLAYEREPDVVLLDIWLKGSDIDGLSVLEKLKER
YPSLPVIMISGHGNIATAVKSLHIGAYDYIEKPFTENRLKLVVKRAVESGRLRRENDELRSFFEDYELIGNSPQIKSLRS
TVNKAASTFSRILITGAPGTGKEVVARLIHKKFRGGSTFVSFCPSILPENSYLENIFGSEGENSSLQHVVPHSVGIIEQA
NHGTLFIDEVTDLRYDAQLRLMRLLQEGRIYRESGKIPVIVDTRVIASSSKIMEDEVRLGKFCEDLYYRLNVFPIRVPSL
SEYCADLPEICEYMMKSICKKMRLFPRAISEEAIVAMQSYCWPGNLRQLRNVLEWVMIMQTKHDVITVDDLPAEIVNGAP
INSVFTHIVSAPLRKAREEFERQYLKTQLSRFGGSVSRTAEFIGMERSALHRKLKMLGLFTG
>Mature_461_residues
SKVKRFYIPEVLIVDDEADLRAMIQDILRDDNYVTRVAADGLSAMKLAYEREPDVVLLDIWLKGSDIDGLSVLEKLKERY
PSLPVIMISGHGNIATAVKSLHIGAYDYIEKPFTENRLKLVVKRAVESGRLRRENDELRSFFEDYELIGNSPQIKSLRST
VNKAASTFSRILITGAPGTGKEVVARLIHKKFRGGSTFVSFCPSILPENSYLENIFGSEGENSSLQHVVPHSVGIIEQAN
HGTLFIDEVTDLRYDAQLRLMRLLQEGRIYRESGKIPVIVDTRVIASSSKIMEDEVRLGKFCEDLYYRLNVFPIRVPSLS
EYCADLPEICEYMMKSICKKMRLFPRAISEEAIVAMQSYCWPGNLRQLRNVLEWVMIMQTKHDVITVDDLPAEIVNGAPI
NSVFTHIVSAPLRKAREEFERQYLKTQLSRFGGSVSRTAEFIGMERSALHRKLKMLGLFTG

Specific function: Member of the two-component regulatory system RBE_0312/RBE_0470 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=473, Percent_Identity=30.4439746300211, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1788550, Length=462, Percent_Identity=31.1688311688312, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1790437, Length=451, Percent_Identity=29.490022172949, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=27.7027027027027, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=30.2469135802469, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI87082152, Length=326, Percent_Identity=29.7546012269939, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082117, Length=341, Percent_Identity=29.9120234604106, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=27.7611940298507, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=30.5993690851735, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1787583, Length=314, Percent_Identity=28.343949044586, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87081872, Length=308, Percent_Identity=29.5454545454545, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=24.6835443037975, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789828, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=129, Percent_Identity=34.1085271317829, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787487, Length=186, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788394, Length=133, Percent_Identity=29.3233082706767, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=34.1666666666667, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52462; Mature: 52331

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKRFYIPEVLIVDDEADLRAMIQDILRDDNYVTRVAADGLSAMKLAYEREPDVVLLD
CCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHCCCCCEEEEE
IWLKGSDIDGLSVLEKLKERYPSLPVIMISGHGNIATAVKSLHIGAYDYIEKPFTENRLK
EEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHCCCCCHHHHH
LVVKRAVESGRLRRENDELRSFFEDYELIGNSPQIKSLRSTVNKAASTFSRILITGAPGT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCCCC
GKEVVARLIHKKFRGGSTFVSFCPSILPENSYLENIFGSEGENSSLQHVVPHSVGIIEQA
CHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHCCCCCCCEEEC
NHGTLFIDEVTDLRYDAQLRLMRLLQEGRIYRESGKIPVIVDTRVIASSSKIMEDEVRLG
CCCEEEEECHHCCCHHHHHHHHHHHHHCCEEECCCCEEEEEECHHHHCCHHHHHHHHHHH
KFCEDLYYRLNVFPIRVPSLSEYCADLPEICEYMMKSICKKMRLFPRAISEEAIVAMQSY
HHHHHHHHHHEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
CWPGNLRQLRNVLEWVMIMQTKHDVITVDDLPAEIVNGAPINSVFTHIVSAPLRKAREEF
CCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ERQYLKTQLSRFGGSVSRTAEFIGMERSALHRKLKMLGLFTG
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKVKRFYIPEVLIVDDEADLRAMIQDILRDDNYVTRVAADGLSAMKLAYEREPDVVLLD
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHCCCCCEEEEE
IWLKGSDIDGLSVLEKLKERYPSLPVIMISGHGNIATAVKSLHIGAYDYIEKPFTENRLK
EEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHCCCCCHHHHH
LVVKRAVESGRLRRENDELRSFFEDYELIGNSPQIKSLRSTVNKAASTFSRILITGAPGT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCCCC
GKEVVARLIHKKFRGGSTFVSFCPSILPENSYLENIFGSEGENSSLQHVVPHSVGIIEQA
CHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHCCCCCCCEEEC
NHGTLFIDEVTDLRYDAQLRLMRLLQEGRIYRESGKIPVIVDTRVIASSSKIMEDEVRLG
CCCEEEEECHHCCCHHHHHHHHHHHHHCCEEECCCCEEEEEECHHHHCCHHHHHHHHHHH
KFCEDLYYRLNVFPIRVPSLSEYCADLPEICEYMMKSICKKMRLFPRAISEEAIVAMQSY
HHHHHHHHHHEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
CWPGNLRQLRNVLEWVMIMQTKHDVITVDDLPAEIVNGAPINSVFTHIVSAPLRKAREEF
CCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ERQYLKTQLSRFGGSVSRTAEFIGMERSALHRKLKMLGLFTG
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA