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The map label for this gene is ribE [H]

Identifier: 88607568

GI number: 88607568

Start: 109222

End: 109833

Strand: Reverse

Name: ribE [H]

Synonym: APH_0106

Alternate gene names: 88607568

Gene position: 109833-109222 (Counterclockwise)

Preceding gene: 88607738

Following gene: 88607011

Centisome position: 7.47

GC content: 43.3

Gene sequence:

>612_bases
ATGTTCAACGGCATAGTTACTCTAATTGGACGTGTGGAAGACATACAACACGACGGAGATACTGATGTTATTGCACGTAT
CAGTGTGCAAAGCGAAGAGTTCTTATCGCAAATTGTCATAGGAAGTTCAATCGCCTGCTCCGGAGTATGCCTGAGCGTAG
TCGAAAAAGGAGCAACTCACTTTGTGACTAACATTTCAAAAGCCACTTTAGACATTACAAATTTGCGATTTTGGACCCTT
GGCACCAAGGTGAATCTAGAACCTGCTTTAAGAGCAGGCGCTCCTTTGGATGGACATATGGTGCAAGGGCACGTTGATTG
CATAGGTGAAATCCATTCCATAACACAGGTTGCTGGTTCGCACGTTATGGAGATATGCTGCGATAGCTCTACATCAAAGT
ACATAGCGAAAAAAAGTTCTATAGCTTTGGATGGAGTATCGCTCACGGTAAATTCTACAACACCTGGGCTGTTCAGCGTA
AACTTGATACCGTATACCTGGAACAATACCACATTCCAATACAATACGGTGGGTGATTTTGTGAATATCGAAACAGATCT
TATAGCGCGATATTTAGAAGCTCTGCTTGCCCATCGCACATATGGCCTATAG

Upstream 100 bases:

>100_bases
CTATCTACTCACACCGCAGAAAATATGTAACAATCATAATGTTCGTGGATACATAGAAGCCTCATCAACCATCGAACTTG
TAGTAAAAGGAGAAATGTAT

Downstream 100 bases:

>100_bases
GCACTTGTGCCCATATACACCGTCTATTACCTCTAAGTATATCTGGATCTTCTGGTTCTATGTAGCTCGCCGGCTGCGTC
AACGAGCATTATAAAAATGC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MFNGIVTLIGRVEDIQHDGDTDVIARISVQSEEFLSQIVIGSSIACSGVCLSVVEKGATHFVTNISKATLDITNLRFWTL
GTKVNLEPALRAGAPLDGHMVQGHVDCIGEIHSITQVAGSHVMEICCDSSTSKYIAKKSSIALDGVSLTVNSTTPGLFSV
NLIPYTWNNTTFQYNTVGDFVNIETDLIARYLEALLAHRTYGL

Sequences:

>Translated_203_residues
MFNGIVTLIGRVEDIQHDGDTDVIARISVQSEEFLSQIVIGSSIACSGVCLSVVEKGATHFVTNISKATLDITNLRFWTL
GTKVNLEPALRAGAPLDGHMVQGHVDCIGEIHSITQVAGSHVMEICCDSSTSKYIAKKSSIALDGVSLTVNSTTPGLFSV
NLIPYTWNNTTFQYNTVGDFVNIETDLIARYLEALLAHRTYGL
>Mature_203_residues
MFNGIVTLIGRVEDIQHDGDTDVIARISVQSEEFLSQIVIGSSIACSGVCLSVVEKGATHFVTNISKATLDITNLRFWTL
GTKVNLEPALRAGAPLDGHMVQGHVDCIGEIHSITQVAGSHVMEICCDSSTSKYIAKKSSIALDGVSLTVNSTTPGLFSV
NLIPYTWNNTTFQYNTVGDFVNIETDLIARYLEALLAHRTYGL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=204, Percent_Identity=33.8235294117647, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319733, Length=205, Percent_Identity=34.6341463414634, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 21934; Mature: 21934

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNGIVTLIGRVEDIQHDGDTDVIARISVQSEEFLSQIVIGSSIACSGVCLSVVEKGATH
CCCCHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
FVTNISKATLDITNLRFWTLGTKVNLEPALRAGAPLDGHMVQGHVDCIGEIHSITQVAGS
HHHCCCHHEEEEECEEEEEECCEECCCHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHH
HVMEICCDSSTSKYIAKKSSIALDGVSLTVNSTTPGLFSVNLIPYTWNNTTFQYNTVGDF
HHHHHHCCCCCHHHHHHCCCEEECCEEEEECCCCCCEEEEEEEEEEECCCEEEEECCCCE
VNIETDLIARYLEALLAHRTYGL
EECCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFNGIVTLIGRVEDIQHDGDTDVIARISVQSEEFLSQIVIGSSIACSGVCLSVVEKGATH
CCCCHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
FVTNISKATLDITNLRFWTLGTKVNLEPALRAGAPLDGHMVQGHVDCIGEIHSITQVAGS
HHHCCCHHEEEEECEEEEEECCEECCCHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHH
HVMEICCDSSTSKYIAKKSSIALDGVSLTVNSTTPGLFSVNLIPYTWNNTTFQYNTVGDF
HHHHHHCCCCCHHHHHHCCCEEECCEEEEECCCCCCEEEEEEEEEEECCCEEEEECCCCE
VNIETDLIARYLEALLAHRTYGL
EECCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]