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The map label for this gene is truA

Identifier: 88607493

GI number: 88607493

Start: 528617

End: 529357

Strand: Reverse

Name: truA

Synonym: APH_0502

Alternate gene names: 88607493

Gene position: 529357-528617 (Counterclockwise)

Preceding gene: 88607329

Following gene: 88607410

Centisome position: 35.98

GC content: 42.24

Gene sequence:

>741_bases
ATGAGATATAAAGCAGTTGTAGAATACGAGGGCACTTCCTTTATAGGCTGGCAGAGGCAACACGGAGTGGTAGGAAAGTC
AGTTCAGGAATCTATAGAGCGCTCTATCAAAGAGTTCTGTCAACAGAGTGTGATAGTTTACGCAGCAGGAAGGACGGATG
CAGGAGTGCATGCAACAGGTCAGGTCGTACATTTTGACCTGAAGGTTTCCCGTGAGGATTACGTTATAAAGAATGCGCTC
AATCACTATCTACGCAATGAAAAGGTGTCAATATTGCAGTTAGAACGGGTTGATGAGGAGTTTCATGCAAGGTTTTCTGC
AAAAAAGCGCCACTACACATATAAAATCTCGAATAGAGAGGCTCCTCTATGTATTGATTTAATGAGAATGTGGCATGTTC
CAAAAAGGTTAAATGTAGAAAATATGCGTGAAGCAGCGTCTTACATTGTCGGAAAGCACGATTTTGCCAGCTTTCGCGCC
AAAGATTGCCAATCTAAGTCTTCAATCAAAACAGTGGATAGTATAGACATCATCGCAGCAGAAGAGGAAATAAACATTAA
TATATCGGCTCAGTCATTTCTACATAAGCAGGTGAGAATAACAGTGGGTACCTTTGTGGAATGCGGCATGGGGCTATATC
CACCTGCACATGTACTGGAAATACTAGAAAAGAAGGATAGAGCAGCTGCAGGAATCACAGCACCAGCCCATGGTCTTTAT
CTAACAAGAGTTGACTATTAA

Upstream 100 bases:

>100_bases
TAGAGATTTCAGATGAAGTAGCTGATGCAAGCTCGTCTATTTTACTGCAAGTAAAGATGGGGGTTGCGGTAAGAAAAGCA
ATTTTACACTATATGTTGGG

Downstream 100 bases:

>100_bases
CGAACGAAGGTAATATGCGCGACACAAAAAGCTCGGAGTATATTATGTTTTTTGTTGAAATCTGAAGAAACTGCTTAGCT
TTACTTGAAGAGGCAGTATG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRYKAVVEYEGTSFIGWQRQHGVVGKSVQESIERSIKEFCQQSVIVYAAGRTDAGVHATGQVVHFDLKVSREDYVIKNAL
NHYLRNEKVSILQLERVDEEFHARFSAKKRHYTYKISNREAPLCIDLMRMWHVPKRLNVENMREAASYIVGKHDFASFRA
KDCQSKSSIKTVDSIDIIAAEEEININISAQSFLHKQVRITVGTFVECGMGLYPPAHVLEILEKKDRAAAGITAPAHGLY
LTRVDY

Sequences:

>Translated_246_residues
MRYKAVVEYEGTSFIGWQRQHGVVGKSVQESIERSIKEFCQQSVIVYAAGRTDAGVHATGQVVHFDLKVSREDYVIKNAL
NHYLRNEKVSILQLERVDEEFHARFSAKKRHYTYKISNREAPLCIDLMRMWHVPKRLNVENMREAASYIVGKHDFASFRA
KDCQSKSSIKTVDSIDIIAAEEEININISAQSFLHKQVRITVGTFVECGMGLYPPAHVLEILEKKDRAAAGITAPAHGLY
LTRVDY
>Mature_246_residues
MRYKAVVEYEGTSFIGWQRQHGVVGKSVQESIERSIKEFCQQSVIVYAAGRTDAGVHATGQVVHFDLKVSREDYVIKNAL
NHYLRNEKVSILQLERVDEEFHARFSAKKRHYTYKISNREAPLCIDLMRMWHVPKRLNVENMREAASYIVGKHDFASFRA
KDCQSKSSIKTVDSIDIIAAEEEININISAQSFLHKQVRITVGTFVECGMGLYPPAHVLEILEKKDRAAAGITAPAHGLY
LTRVDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=31.3868613138686, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788657, Length=240, Percent_Identity=38.75, Blast_Score=175, Evalue=3e-45,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=28.8079470198675, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_ANAPZ (Q2GKK4)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505097.1
- ProteinModelPortal:   Q2GKK4
- SMR:   Q2GKK4
- STRING:   Q2GKK4
- GeneID:   3931158
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0502
- NMPDR:   fig|212042.5.peg.496
- TIGR:   APH_0502
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   HSAIRRH
- PhylomeDB:   Q2GKK4
- ProtClustDB:   PRK00021
- BioCyc:   APHA212042:APH_0502-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28030; Mature: 28030

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKAVVEYEGTSFIGWQRQHGVVGKSVQESIERSIKEFCQQSVIVYAAGRTDAGVHATG
CCCEEEEEECCCEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEECC
QVVHFDLKVSREDYVIKNALNHYLRNEKVSILQLERVDEEFHARFSAKKRHYTYKISNRE
EEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHCCCCEEEEEECCCC
APLCIDLMRMWHVPKRLNVENMREAASYIVGKHDFASFRAKDCQSKSSIKTVDSIDIIAA
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEE
EEEININISAQSFLHKQVRITVGTFVECGMGLYPPAHVLEILEKKDRAAAGITAPAHGLY
CCEEEEEEEHHHHHHHHHEEEEHHHHHCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCEE
LTRVDY
EEEECC
>Mature Secondary Structure
MRYKAVVEYEGTSFIGWQRQHGVVGKSVQESIERSIKEFCQQSVIVYAAGRTDAGVHATG
CCCEEEEEECCCEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEECC
QVVHFDLKVSREDYVIKNALNHYLRNEKVSILQLERVDEEFHARFSAKKRHYTYKISNRE
EEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHCCCCEEEEEECCCC
APLCIDLMRMWHVPKRLNVENMREAASYIVGKHDFASFRAKDCQSKSSIKTVDSIDIIAA
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEE
EEEININISAQSFLHKQVRITVGTFVECGMGLYPPAHVLEILEKKDRAAAGITAPAHGLY
CCEEEEEEEHHHHHHHHHEEEEHHHHHCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCEE
LTRVDY
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA