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The map label for this gene is mnmG

Identifier: 88607384

GI number: 88607384

Start: 221234

End: 223120

Strand: Reverse

Name: mnmG

Synonym: APH_0224

Alternate gene names: 88607384

Gene position: 223120-221234 (Counterclockwise)

Preceding gene: 88607534

Following gene: 88607287

Centisome position: 15.17

GC content: 43.4

Gene sequence:

>1887_bases
GTGCAAAACTATGATGTAGTAGTTGTTGGTGGAGGACATGCTGGTTGCGAGGCCGCTGCCGCTGCTGCACGAGTAGGTGC
TAAAACTTTATTGGTTACACATAAAGTAGAATCCATAGGAGAGATGTCCTGCAATCCCGCTATAGGTGGTATTGCGAAAG
GAGTAGTAGTGCGTGAGGTCGATGCGTTAGACGGCCTTATGGGAAAGGTCATCGATAAATCTAGTATTCACTCTATAATC
CTCAATAGAAGTAAAGGACCAGCCGTATGGGGACCAAGAGCACAGGCAGATAGGGAAGTTTACAAGCCTACTATGCGTGA
TATGGTGCTACACTACGAAAACTTAACAGTTATATCAGAGGAAGTAGTTGACTTTACCGTTGATGGTGTAGAAGAACGCC
CTCAGATCTCCTCGGTATTGCTGTCGAATGGAGAGTTGATAAAAACTACCAGATTAGTTTTTGCTACAGGAACCTTCTTA
GGAGGGACAATACACGTAGGAAATGAAAGCTTTCCAGCAGGCAGGATAGGAGACAAACCCTCCACAAAGCTTCCACAAGC
CCTGACAGCACATGGATTTCAATTGGGTAGATTGAAAACTGGCACTCCTCCGAGAATTGATAGAGACAGTATAAATTGGT
CAAAACTCTTGGAGCAAAAGGGAGATGTGCTTCCGACGCCATTTTCTTTTATGTCTGAGTGCGTGAGTTTGCCTCAAATA
TCTTGCTACGTGACTCATACCAATGAAAAAACTCATGAAATCATTAGAAGGAACCTACATCTAGCGGGCTCACGGTGCGA
GTCTCTCTTAGATGTAATGGCTCCTAGGTATTGCCCCTCTATAGAAGAAAAGATACGCAGATTTCCAGACCACAAAAGCC
ATCAGATTTTCCTTGAACCCGAGGGACTTGAAACTAACTCTGTATACCCAAATGGTATATCTACTTCTTGTCCTATTGAA
GTGCAGCTTGAGATGCTGAGGAGTATTAATGGACTAGAACAGGCTGTGATGCTCCGCCATGGATACACTGTGGAATACAA
CTTCATAGATCCTCGCGAGTTGTACCATACGTTAGAGACTAAAAAAATTAAAGGGCTCTTCTGCGCAGGGCAAATTAATG
GCACTACAGGGTATGAAGAAGCTGCCGGACAGGGTATCGTTGCCGGAGCAAATGCCGCGCTTTCTTTAGTGCAAAATCAG
GAACCTCTGGTTTTGAAGCGTAGTGACTCATACATAGGAGTGATGATAGATGATCTTGTAACATTAGGTACAAGCGAGCC
TTATAGGCTTTTTACATCACGTGCTGAGTATAGGCTAACTCTGCGCTCTGATAATGCTGATATGCGCTTAACTGAGATCG
GACGTGCGTACTCACTAGTATCTCAAGAGCGTTTTGATCGCTTATCTCAAAAAAAACAAGAGATGCATGCTTTGCTGAAT
GCTCTAAAGGGTATCGTAGCCACACCCAACGATATTGCAGAATATGACATTGCCATTGCACAGAATGGTGAAAAGAAAAA
TGCCTTTGAATTGCTCAGTCACCCTAACATAAACATGGAAGTACTGTTAAAAATATGGCCAAGTCTAAGATCTTTTAGTG
CTGGTACACTGGCTTTAATGGAAATTGAAGGAAAGTACGCACCTTATTTGAAGCGGCAGGAGGCTGACATCAAATCATTC
CTAGAGGAAGAAAATTTGTCTATACCTAAAGATATTCTTTACTCTGACGTGTACGGGTTATCAAAAGAGGCACAGGAGAA
ACTACAAGCAGTCAAGCCGTTTTCCATAGGCGCAGCAAGAAGAATACCAGGTATTACACCAGCGGCTATCGCGAATATCT
TGATACACTTACGCTGCAAGCATAAGTCCTCACATAAGTCACTGTAA

Upstream 100 bases:

>100_bases
CATCTACCTAATAAACTCATGAATTTTAGCATCATTTGACATGACTTATATTACTTTTTACCATCTATCGAGTATATTTT
AGTAATAACGGTAGGTTGTA

Downstream 100 bases:

>100_bases
ACAGCATTCGCAACCACGTGTGCTTCTTTCTGTTTCTGTATTTGTATGCGCCCCATATTAGAATAAGGAATCTTCGCTGT
ATAGCAACGTAGCTGCAGTG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MQNYDVVVVGGGHAGCEAAAAAARVGAKTLLVTHKVESIGEMSCNPAIGGIAKGVVVREVDALDGLMGKVIDKSSIHSII
LNRSKGPAVWGPRAQADREVYKPTMRDMVLHYENLTVISEEVVDFTVDGVEERPQISSVLLSNGELIKTTRLVFATGTFL
GGTIHVGNESFPAGRIGDKPSTKLPQALTAHGFQLGRLKTGTPPRIDRDSINWSKLLEQKGDVLPTPFSFMSECVSLPQI
SCYVTHTNEKTHEIIRRNLHLAGSRCESLLDVMAPRYCPSIEEKIRRFPDHKSHQIFLEPEGLETNSVYPNGISTSCPIE
VQLEMLRSINGLEQAVMLRHGYTVEYNFIDPRELYHTLETKKIKGLFCAGQINGTTGYEEAAGQGIVAGANAALSLVQNQ
EPLVLKRSDSYIGVMIDDLVTLGTSEPYRLFTSRAEYRLTLRSDNADMRLTEIGRAYSLVSQERFDRLSQKKQEMHALLN
ALKGIVATPNDIAEYDIAIAQNGEKKNAFELLSHPNINMEVLLKIWPSLRSFSAGTLALMEIEGKYAPYLKRQEADIKSF
LEEENLSIPKDILYSDVYGLSKEAQEKLQAVKPFSIGAARRIPGITPAAIANILIHLRCKHKSSHKSL

Sequences:

>Translated_628_residues
MQNYDVVVVGGGHAGCEAAAAAARVGAKTLLVTHKVESIGEMSCNPAIGGIAKGVVVREVDALDGLMGKVIDKSSIHSII
LNRSKGPAVWGPRAQADREVYKPTMRDMVLHYENLTVISEEVVDFTVDGVEERPQISSVLLSNGELIKTTRLVFATGTFL
GGTIHVGNESFPAGRIGDKPSTKLPQALTAHGFQLGRLKTGTPPRIDRDSINWSKLLEQKGDVLPTPFSFMSECVSLPQI
SCYVTHTNEKTHEIIRRNLHLAGSRCESLLDVMAPRYCPSIEEKIRRFPDHKSHQIFLEPEGLETNSVYPNGISTSCPIE
VQLEMLRSINGLEQAVMLRHGYTVEYNFIDPRELYHTLETKKIKGLFCAGQINGTTGYEEAAGQGIVAGANAALSLVQNQ
EPLVLKRSDSYIGVMIDDLVTLGTSEPYRLFTSRAEYRLTLRSDNADMRLTEIGRAYSLVSQERFDRLSQKKQEMHALLN
ALKGIVATPNDIAEYDIAIAQNGEKKNAFELLSHPNINMEVLLKIWPSLRSFSAGTLALMEIEGKYAPYLKRQEADIKSF
LEEENLSIPKDILYSDVYGLSKEAQEKLQAVKPFSIGAARRIPGITPAAIANILIHLRCKHKSSHKSL
>Mature_628_residues
MQNYDVVVVGGGHAGCEAAAAAARVGAKTLLVTHKVESIGEMSCNPAIGGIAKGVVVREVDALDGLMGKVIDKSSIHSII
LNRSKGPAVWGPRAQADREVYKPTMRDMVLHYENLTVISEEVVDFTVDGVEERPQISSVLLSNGELIKTTRLVFATGTFL
GGTIHVGNESFPAGRIGDKPSTKLPQALTAHGFQLGRLKTGTPPRIDRDSINWSKLLEQKGDVLPTPFSFMSECVSLPQI
SCYVTHTNEKTHEIIRRNLHLAGSRCESLLDVMAPRYCPSIEEKIRRFPDHKSHQIFLEPEGLETNSVYPNGISTSCPIE
VQLEMLRSINGLEQAVMLRHGYTVEYNFIDPRELYHTLETKKIKGLFCAGQINGTTGYEEAAGQGIVAGANAALSLVQNQ
EPLVLKRSDSYIGVMIDDLVTLGTSEPYRLFTSRAEYRLTLRSDNADMRLTEIGRAYSLVSQERFDRLSQKKQEMHALLN
ALKGIVATPNDIAEYDIAIAQNGEKKNAFELLSHPNINMEVLLKIWPSLRSFSAGTLALMEIEGKYAPYLKRQEADIKSF
LEEENLSIPKDILYSDVYGLSKEAQEKLQAVKPFSIGAARRIPGITPAAIANILIHLRCKHKSSHKSL

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=637, Percent_Identity=46.1538461538462, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI19882217, Length=654, Percent_Identity=44.954128440367, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI183227703, Length=669, Percent_Identity=43.9461883408072, Blast_Score=508, Evalue=1e-144,
Organism=Escherichia coli, GI2367273, Length=621, Percent_Identity=48.4702093397746, Blast_Score=560, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI17534255, Length=626, Percent_Identity=43.2907348242812, Blast_Score=502, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6321202, Length=635, Percent_Identity=45.8267716535433, Blast_Score=556, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24658174, Length=628, Percent_Identity=48.2484076433121, Blast_Score=556, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_ANAPZ (Q2GLA8)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504843.1
- ProteinModelPortal:   Q2GLA8
- SMR:   Q2GLA8
- STRING:   Q2GLA8
- GeneID:   3931118
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0224
- NMPDR:   fig|212042.5.peg.214
- TIGR:   APH_0224
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q2GLA8
- ProtClustDB:   PRK05192
- BioCyc:   APHA212042:APH_0224-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69246; Mature: 69246

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 122-122 BINDING 181-181 BINDING 371-371

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNYDVVVVGGGHAGCEAAAAAARVGAKTLLVTHKVESIGEMSCNPAIGGIAKGVVVREV
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCHHHHCHHHHHH
DALDGLMGKVIDKSSIHSIILNRSKGPAVWGPRAQADREVYKPTMRDMVLHYENLTVISE
HHHHHHHHHHHCHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHH
EVVDFTVDGVEERPQISSVLLSNGELIKTTRLVFATGTFLGGTIHVGNESFPAGRIGDKP
HHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEEEEECCEECCEEEECCCCCCCCCCCCCC
STKLPQALTAHGFQLGRLKTGTPPRIDRDSINWSKLLEQKGDVLPTPFSFMSECVSLPQI
CCCCCHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCE
SCYVTHTNEKTHEIIRRNLHLAGSRCESLLDVMAPRYCPSIEEKIRRFPDHKSHQIFLEP
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEECC
EGLETNSVYPNGISTSCPIEVQLEMLRSINGLEQAVMLRHGYTVEYNFIDPRELYHTLET
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
KKIKGLFCAGQINGTTGYEEAAGQGIVAGANAALSLVQNQEPLVLKRSDSYIGVMIDDLV
HHCCEEEEEEECCCCCCCHHHCCCCEEECCHHHHHHHCCCCCEEEEECCCEEEEEHHHHH
TLGTSEPYRLFTSRAEYRLTLRSDNADMRLTEIGRAYSLVSQERFDRLSQKKQEMHALLN
HCCCCCCHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKGIVATPNDIAEYDIAIAQNGEKKNAFELLSHPNINMEVLLKIWPSLRSFSAGTLALM
HHHCCCCCCCHHHHHEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEE
EIEGKYAPYLKRQEADIKSFLEEENLSIPKDILYSDVYGLSKEAQEKLQAVKPFSIGAAR
EECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHH
RIPGITPAAIANILIHLRCKHKSSHKSL
CCCCCCHHHHHHHHEEEEECCCCCCCCC
>Mature Secondary Structure
MQNYDVVVVGGGHAGCEAAAAAARVGAKTLLVTHKVESIGEMSCNPAIGGIAKGVVVREV
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCHHHHCHHHHHH
DALDGLMGKVIDKSSIHSIILNRSKGPAVWGPRAQADREVYKPTMRDMVLHYENLTVISE
HHHHHHHHHHHCHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHH
EVVDFTVDGVEERPQISSVLLSNGELIKTTRLVFATGTFLGGTIHVGNESFPAGRIGDKP
HHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEEEEECCEECCEEEECCCCCCCCCCCCCC
STKLPQALTAHGFQLGRLKTGTPPRIDRDSINWSKLLEQKGDVLPTPFSFMSECVSLPQI
CCCCCHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCE
SCYVTHTNEKTHEIIRRNLHLAGSRCESLLDVMAPRYCPSIEEKIRRFPDHKSHQIFLEP
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEECC
EGLETNSVYPNGISTSCPIEVQLEMLRSINGLEQAVMLRHGYTVEYNFIDPRELYHTLET
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
KKIKGLFCAGQINGTTGYEEAAGQGIVAGANAALSLVQNQEPLVLKRSDSYIGVMIDDLV
HHCCEEEEEEECCCCCCCHHHCCCCEEECCHHHHHHHCCCCCEEEEECCCEEEEEHHHHH
TLGTSEPYRLFTSRAEYRLTLRSDNADMRLTEIGRAYSLVSQERFDRLSQKKQEMHALLN
HCCCCCCHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKGIVATPNDIAEYDIAIAQNGEKKNAFELLSHPNINMEVLLKIWPSLRSFSAGTLALM
HHHCCCCCCCHHHHHEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEE
EIEGKYAPYLKRQEADIKSFLEEENLSIPKDILYSDVYGLSKEAQEKLQAVKPFSIGAAR
EECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHH
RIPGITPAAIANILIHLRCKHKSSHKSL
CCCCCCHHHHHHHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA