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The map label for this gene is clpP

Identifier: 88607298

GI number: 88607298

Start: 1031432

End: 1032025

Strand: Reverse

Name: clpP

Synonym: APH_0970

Alternate gene names: 88607298

Gene position: 1032025-1031432 (Counterclockwise)

Preceding gene: 88607838

Following gene: 88607602

Centisome position: 70.14

GC content: 44.28

Gene sequence:

>594_bases
ATGAACTTGGTTCCGATGGTAGTTGAGCAGACTGGTAGGGGTGAGCGGGCGTATGATATCTATTCTAGATTGCTGAAAGA
GAGGATTATATTCGTTACGGGGCCTATCGAAGATGAGATGGCAAGTCTCATAGTTGCGCAGTTAGTATTTCTGGAAGCTG
AAGATCCTGAGAAGGATATATCCATGTATATAAATTCGCCAGGTGGTGTGGTGACGGCTGGGCTATCTATATACGACACT
ATGCAGTATATAAAGCCGAATGTTGCTACGCTTTGTTTGGGGCAAGCGGCATCTATGGGGTCTCTTTTGTTGTGTGCAGG
GGCGCCTGGGATGCGTTGTGCACTTCCGAATTCTAGAGTTATGATTCATCAGCCATCTGGTGGTTTTCGTGGACAGGCTA
CTGATATAGAGATACACGCTAGAGAGATATTGGAGATAAAACGTAGGCTAAATGAGATTTTCGTTCGGCATACTGGTAAA
TCTTTGGAAGAGATAGAGAGTAGCATGGAAAGGGATAATTTCATGATTGCCGAGAAGGCGCGGGATTTCGGTATTATAGA
TAAGGTGATAGAAAAGCGTATTGAGGAAAAGTAG

Upstream 100 bases:

>100_bases
AGCATGTCTGCGGGGGAGCTTAAATCGCTTTTTGAGAGTGTGTAGGTAATAGTTTTAAGCTTGTCTTGAGGGTTTGTGTA
TGGGATTTGGGGGGTAGTCT

Downstream 100 bases:

>100_bases
TAGGAAGGTAAATGTCTGAGACGAAGAAAGGCGCTTATAGTTGTTCATTTTGCGGTAAGTTGCATAGTGAAGTAAAGAAG
CTGATAGCCGGTCCTGAGGC

Product: ATP-dependent Clp protease proteolytic subunit

Products: NA

Alternate protein names: Endopeptidase Clp

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MNLVPMVVEQTGRGERAYDIYSRLLKERIIFVTGPIEDEMASLIVAQLVFLEAEDPEKDISMYINSPGGVVTAGLSIYDT
MQYIKPNVATLCLGQAASMGSLLLCAGAPGMRCALPNSRVMIHQPSGGFRGQATDIEIHAREILEIKRRLNEIFVRHTGK
SLEEIESSMERDNFMIAEKARDFGIIDKVIEKRIEEK

Sequences:

>Translated_197_residues
MNLVPMVVEQTGRGERAYDIYSRLLKERIIFVTGPIEDEMASLIVAQLVFLEAEDPEKDISMYINSPGGVVTAGLSIYDT
MQYIKPNVATLCLGQAASMGSLLLCAGAPGMRCALPNSRVMIHQPSGGFRGQATDIEIHAREILEIKRRLNEIFVRHTGK
SLEEIESSMERDNFMIAEKARDFGIIDKVIEKRIEEK
>Mature_197_residues
MNLVPMVVEQTGRGERAYDIYSRLLKERIIFVTGPIEDEMASLIVAQLVFLEAEDPEKDISMYINSPGGVVTAGLSIYDT
MQYIKPNVATLCLGQAASMGSLLLCAGAPGMRCALPNSRVMIHQPSGGFRGQATDIEIHAREILEIKRRLNEIFVRHTGK
SLEEIESSMERDNFMIAEKARDFGIIDKVIEKRIEEK

Specific function: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family

Homologues:

Organism=Homo sapiens, GI5174419, Length=190, Percent_Identity=66.8421052631579, Blast_Score=272, Evalue=1e-73,
Organism=Escherichia coli, GI1786641, Length=193, Percent_Identity=68.3937823834197, Blast_Score=290, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17538017, Length=186, Percent_Identity=58.6021505376344, Blast_Score=239, Evalue=7e-64,
Organism=Drosophila melanogaster, GI20129427, Length=191, Percent_Identity=67.0157068062827, Blast_Score=275, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLPP_ANAPZ (Q2GJB4)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505537.1
- ProteinModelPortal:   Q2GJB4
- SMR:   Q2GJB4
- STRING:   Q2GJB4
- MEROPS:   S14.001
- GeneID:   3930516
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0970
- NMPDR:   fig|212042.5.peg.916
- TIGR:   APH_0970
- eggNOG:   COG0740
- HOGENOM:   HBG558421
- OMA:   LPNASIM
- PhylomeDB:   Q2GJB4
- ProtClustDB:   PRK00277
- BioCyc:   APHA212042:APH_0970-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00444
- InterPro:   IPR001907
- InterPro:   IPR018215
- PANTHER:   PTHR10381
- PRINTS:   PR00127
- TIGRFAMs:   TIGR00493

Pfam domain/function: PF00574 CLP_protease

EC number: =3.4.21.92

Molecular weight: Translated: 21990; Mature: 21990

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00382 CLP_PROTEASE_HIS; PS00381 CLP_PROTEASE_SER

Important sites: ACT_SITE 98-98 ACT_SITE 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVPMVVEQTGRGERAYDIYSRLLKERIIFVTGPIEDEMASLIVAQLVFLEAEDPEKDI
CCCCCHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCE
SMYINSPGGVVTAGLSIYDTMQYIKPNVATLCLGQAASMGSLLLCAGAPGMRCALPNSRV
EEEECCCCCEEEECHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCEEECCCCEE
MIHQPSGGFRGQATDIEIHAREILEIKRRLNEIFVRHTGKSLEEIESSMERDNFMIAEKA
EEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEHHH
RDFGIIDKVIEKRIEEK
CCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLVPMVVEQTGRGERAYDIYSRLLKERIIFVTGPIEDEMASLIVAQLVFLEAEDPEKDI
CCCCCHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCE
SMYINSPGGVVTAGLSIYDTMQYIKPNVATLCLGQAASMGSLLLCAGAPGMRCALPNSRV
EEEECCCCCEEEECHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCEEECCCCEE
MIHQPSGGFRGQATDIEIHAREILEIKRRLNEIFVRHTGKSLEEIESSMERDNFMIAEKA
EEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEHHH
RDFGIIDKVIEKRIEEK
CCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA