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The map label for this gene is glyA

Identifier: 88607134

GI number: 88607134

Start: 155752

End: 157029

Strand: Direct

Name: glyA

Synonym: APH_0154

Alternate gene names: 88607134

Gene position: 155752-157029 (Clockwise)

Preceding gene: 88606906

Following gene: 88607682

Centisome position: 10.59

GC content: 48.36

Gene sequence:

>1278_bases
ATGGTTGGGTACATTGGGGACATCCATATAAGTGAGAGTGATGCTGAGGTTGCTGAGTGTTTATCTGCTGAGTATAAGCG
GCAGAATACGTCTCTGCAGATGATAGCTTCGGAGAATTTTGTTAGCCGTGCTGTTCTGCAGGCTCAAGGATCGGTCCTAA
CCAACAAATATGCGGAGGGTTATCCCGGTAGTAGGTATTACTGTGGTTGTTCTGAGGTAGACGTTGCGGAGACTTTGGCT
GTGGAGCGTCTATGTAAGTTATTTGGCTGTAAGTACGCTAATGTTCAGCCGCACTCAGGGTCGCAGGCGAATCAGCAAGT
GTACATGGCGTTATTAAAGCCCGGAGACACCGTGCTAGGGATGTCATTGGACTCGGGAGGGCACTTAACGCATGGAGCGG
GGCCTAATGTATCGGGGAAGTGGTTTAATGCTGTGCCCTATAATGTTAGGCGTGACACAAACCTTCTGGATATGGGTGAG
ATAGAAGAGATTGCTTTGAGGGTGAAGCCTAATTTGATCATAGCAGGTGCTTCCTCGTATCCTAGACGAATAGACTTCAA
GGCCTTCAGGGCTATAGCTGATAAGGTGGGGGCATATTTCCTAGCGGATATAGCGCATTACTCAGGGTTAATAGCAGGAG
GTCAGTATCCGACGCCATTTGGCTATGCGCATGTAGTGACGTCGACTACGCACAAGACGCTACGTGGTCCTCGCGGAGGC
GTGATAATGACAGATGATGAGGAAATACACAAAAAGTTAAGGTCGGCGGTATTTCCTGGCATGCAGGGAGGGGCTCTGAT
GCATGTAATAGCGGCGAAAGCGGTTGCTTTTCGTGAGGCAATGAGTCCGGATTTTAAGGTATATGTTAGCCAGATTCTTG
ATAATTCTCGTGCTTTAGCAGCAGTTTTAGCTACTGGAGGGTTGGATGTAGTAACTGGTGGGACTGATAGCCATATGGTG
GTTGTTGATTTGCGCTCCAAGGGGCTCACTGGCAGGGATGTTTCGTCTTCGTTGGAGAGAGCTGGGATTGTATGTAATAA
AAATGCTGTTCCATTTGATACTGAAAAGCCGTGGGTAACCTCTGGTATTAGGTTAGGTGCTGCTGCTGAAACTTCTAGAG
GGTTGGTGGTCAAGGATTTTGAGAAGATAGGGCAGCTTGTATTGAAGATTGTGGACTCTATGAGGGCTGGAGCGGATATG
TCCGTAGTGGAGTCGGGGGTTAGAGAAGAGGTTGCTACTCTTGTTAGGGTAGTGCCGTACGATACTTTGGCTTGCTAG

Upstream 100 bases:

>100_bases
GGGGGAGATGAGGTTGTGTCTGTTGATTCGGTTGCTGCTGAAGATGGTGGTGCCGATGCTAGCGGTGGTACTGCTTAGGT
TATTTTGAGGGAATTTGTCT

Downstream 100 bases:

>100_bases
GTGTTTTAGCTGTTGTTGGCGTGAGCTTAAGTATTGTTGGTTTTGAGTATGCGCTTGTTTGTCGGATGTTAGTTCTCTGC
GGTTAGATTGCGTTCTAGCG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MVGYIGDIHISESDAEVAECLSAEYKRQNTSLQMIASENFVSRAVLQAQGSVLTNKYAEGYPGSRYYCGCSEVDVAETLA
VERLCKLFGCKYANVQPHSGSQANQQVYMALLKPGDTVLGMSLDSGGHLTHGAGPNVSGKWFNAVPYNVRRDTNLLDMGE
IEEIALRVKPNLIIAGASSYPRRIDFKAFRAIADKVGAYFLADIAHYSGLIAGGQYPTPFGYAHVVTSTTHKTLRGPRGG
VIMTDDEEIHKKLRSAVFPGMQGGALMHVIAAKAVAFREAMSPDFKVYVSQILDNSRALAAVLATGGLDVVTGGTDSHMV
VVDLRSKGLTGRDVSSSLERAGIVCNKNAVPFDTEKPWVTSGIRLGAAAETSRGLVVKDFEKIGQLVLKIVDSMRAGADM
SVVESGVREEVATLVRVVPYDTLAC

Sequences:

>Translated_425_residues
MVGYIGDIHISESDAEVAECLSAEYKRQNTSLQMIASENFVSRAVLQAQGSVLTNKYAEGYPGSRYYCGCSEVDVAETLA
VERLCKLFGCKYANVQPHSGSQANQQVYMALLKPGDTVLGMSLDSGGHLTHGAGPNVSGKWFNAVPYNVRRDTNLLDMGE
IEEIALRVKPNLIIAGASSYPRRIDFKAFRAIADKVGAYFLADIAHYSGLIAGGQYPTPFGYAHVVTSTTHKTLRGPRGG
VIMTDDEEIHKKLRSAVFPGMQGGALMHVIAAKAVAFREAMSPDFKVYVSQILDNSRALAAVLATGGLDVVTGGTDSHMV
VVDLRSKGLTGRDVSSSLERAGIVCNKNAVPFDTEKPWVTSGIRLGAAAETSRGLVVKDFEKIGQLVLKIVDSMRAGADM
SVVESGVREEVATLVRVVPYDTLAC
>Mature_425_residues
MVGYIGDIHISESDAEVAECLSAEYKRQNTSLQMIASENFVSRAVLQAQGSVLTNKYAEGYPGSRYYCGCSEVDVAETLA
VERLCKLFGCKYANVQPHSGSQANQQVYMALLKPGDTVLGMSLDSGGHLTHGAGPNVSGKWFNAVPYNVRRDTNLLDMGE
IEEIALRVKPNLIIAGASSYPRRIDFKAFRAIADKVGAYFLADIAHYSGLIAGGQYPTPFGYAHVVTSTTHKTLRGPRGG
VIMTDDEEIHKKLRSAVFPGMQGGALMHVIAAKAVAFREAMSPDFKVYVSQILDNSRALAAVLATGGLDVVTGGTDSHMV
VVDLRSKGLTGRDVSSSLERAGIVCNKNAVPFDTEKPWVTSGIRLGAAAETSRGLVVKDFEKIGQLVLKIVDSMRAGADM
SVVESGVREEVATLVRVVPYDTLAC

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=404, Percent_Identity=44.8019801980198, Blast_Score=321, Evalue=8e-88,
Organism=Homo sapiens, GI261862350, Length=404, Percent_Identity=44.8019801980198, Blast_Score=321, Evalue=8e-88,
Organism=Homo sapiens, GI261862348, Length=404, Percent_Identity=44.8019801980198, Blast_Score=321, Evalue=8e-88,
Organism=Homo sapiens, GI19923315, Length=404, Percent_Identity=44.8019801980198, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI22547186, Length=452, Percent_Identity=42.6991150442478, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI261862346, Length=404, Percent_Identity=44.0594059405941, Blast_Score=308, Evalue=7e-84,
Organism=Homo sapiens, GI22547189, Length=438, Percent_Identity=41.324200913242, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1788902, Length=400, Percent_Identity=52.25, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25144732, Length=401, Percent_Identity=44.139650872818, Blast_Score=331, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI25144729, Length=401, Percent_Identity=44.139650872818, Blast_Score=331, Evalue=6e-91,
Organism=Saccharomyces cerevisiae, GI6323087, Length=407, Percent_Identity=42.997542997543, Blast_Score=325, Evalue=7e-90,
Organism=Saccharomyces cerevisiae, GI6319739, Length=412, Percent_Identity=40.0485436893204, Blast_Score=311, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24640005, Length=396, Percent_Identity=45.2020202020202, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI221329721, Length=396, Percent_Identity=45.2020202020202, Blast_Score=338, Evalue=4e-93,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_ANAPZ (Q2GLH3)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504778.1
- HSSP:   P34897
- ProteinModelPortal:   Q2GLH3
- SMR:   Q2GLH3
- STRING:   Q2GLH3
- GeneID:   3930419
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0154
- NMPDR:   fig|212042.5.peg.148
- TIGR:   APH_0154
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   IDMEQVA
- ProtClustDB:   PRK00011
- BioCyc:   APHA212042:APH_0154-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45576; Mature: 45576

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGYIGDIHISESDAEVAECLSAEYKRQNTSLQMIASENFVSRAVLQAQGSVLTNKYAEG
CCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCC
YPGSRYYCGCSEVDVAETLAVERLCKLFGCKYANVQPHSGSQANQQVYMALLKPGDTVLG
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEE
MSLDSGGHLTHGAGPNVSGKWFNAVPYNVRRDTNLLDMGEIEEIALRVKPNLIIAGASSY
EEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHEEECCCEEEECCCCC
PRRIDFKAFRAIADKVGAYFLADIAHYSGLIAGGQYPTPFGYAHVVTSTTHKTLRGPRGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEEECCHHHHHCCCCCC
VIMTDDEEIHKKLRSAVFPGMQGGALMHVIAAKAVAFREAMSPDFKVYVSQILDNSRALA
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
AVLATGGLDVVTGGTDSHMVVVDLRSKGLTGRDVSSSLERAGIVCNKNAVPFDTEKPWVT
HHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCCEEECCCCCCCCCCCCCCC
SGIRLGAAAETSRGLVVKDFEKIGQLVLKIVDSMRAGADMSVVESGVREEVATLVRVVPY
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
DTLAC
HHCCC
>Mature Secondary Structure
MVGYIGDIHISESDAEVAECLSAEYKRQNTSLQMIASENFVSRAVLQAQGSVLTNKYAEG
CCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCC
YPGSRYYCGCSEVDVAETLAVERLCKLFGCKYANVQPHSGSQANQQVYMALLKPGDTVLG
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEE
MSLDSGGHLTHGAGPNVSGKWFNAVPYNVRRDTNLLDMGEIEEIALRVKPNLIIAGASSY
EEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHEEECCCEEEECCCCC
PRRIDFKAFRAIADKVGAYFLADIAHYSGLIAGGQYPTPFGYAHVVTSTTHKTLRGPRGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEEECCHHHHHCCCCCC
VIMTDDEEIHKKLRSAVFPGMQGGALMHVIAAKAVAFREAMSPDFKVYVSQILDNSRALA
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
AVLATGGLDVVTGGTDSHMVVVDLRSKGLTGRDVSSSLERAGIVCNKNAVPFDTEKPWVT
HHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCCEEECCCCCCCCCCCCCCC
SGIRLGAAAETSRGLVVKDFEKIGQLVLKIVDSMRAGADMSVVESGVREEVATLVRVVPY
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
DTLAC
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA