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The map label for this gene is proS

Identifier: 88607069

GI number: 88607069

Start: 669860

End: 671140

Strand: Reverse

Name: proS

Synonym: APH_0647

Alternate gene names: 88607069

Gene position: 671140-669860 (Counterclockwise)

Preceding gene: 88607751

Following gene: 88606943

Centisome position: 45.62

GC content: 41.22

Gene sequence:

>1281_bases
ATGCGTCTGTCGGAGTTTTATTCTCCTACTGTTAAGAACGTGTCATCGGATGTCGTTTCTGCATCTCATAAATATTCCAT
TCGTGCTGGTATTGTATCGCAGACTGCTTCTGGCATCTATACTTTGCTACCATTAGGCTTAATGGTGCTGAGAAAGGTGG
AAAATATCATCCGTGAGGAAATAAATGCAGTAGGCTTTTCAGAGATCTTAATGCCTACTATGCAGCCTGCAGATTTGTGG
AAAGAATCACAAAGATACGATTCTTACGGTCAGGAATTGATCCGTATTCATGATCGTGGCGGCAGAGAAATGGTTTTAGG
CCCTACGCATGAGGAAGTTGTTACTGATTTGGTTAGGTCTTCATTAAAAAGCTATAGAGATCTGCCAGTAAATTTATATC
AGATTCAGTGGAAATTCCGCGATGAGTTACGCCCTAGAAATGGCATACTCCGTAGCAGAGAGTTTTTAATGATGGATGCG
TATAGCTTCGATACCGACTTTGAGAAGGCTATGAAAACCTATGATGCAGTATTCCGGGCATATAGGAAAGCTTTTAAAAG
AATGAATCTGCAGACTATAGCATTAAAAGCTGATATGGGTGCTATAGGCGGAAGTGTAAGCCATGAATTTCATGTTTTGA
CTCCTACGGGAGAAAGCACTGTCTACTATGACGAGCGGGCACTAGAGTTGTCGGAGATGAATGATTATGGCATTGAAGAA
TTGAAAGAGGTATATGCCGCAACCGATGATATGCATGACGAAAAAAGTTGCGGTATTGCTCCTGAAGACTTAAAAACTGC
GCGTGGTATAGAAGTAGGACACATTTTTTATCTTGATGATCGTTATTCTAGAACAATGAATGTGAAGTTCTGTAATACTG
ATGGGCATAGTGGTACACATGTAAAGATGGGTTGCTACGGCATCGGAATTTCGAGGCTAATTGGGGCACTAATAGAGGTG
TTTCATGATGATGCGGGAATCAAGTGGCCCCTGTCTGTTGCGCCGTTTAAGGTTGGTATTGTCAATCTTTTTTCGAAGAA
TGAAGAGTGCAAAAGAGTATCAGAACGCATACATTCCGTGCTCCCTAATGATTCTTTATATGACGATAGAGATGATACTC
CTGGGGTAAAGTTGTCGAGAATGGACCTCATAGGTTTGCCTTGGCAAGTGATTGTGGGAAACTCCTTTATCAAGGATGGT
GTATTAGAGTTAAAAAATCGTGCAACTGGTGATATAGAGCTCCTTTCTGTTGATGATGTAGTTTCTAGGATTAGCGTATG
A

Upstream 100 bases:

>100_bases
ATACTTCCCTTATTCTCTATAGCAAGCTGTGGGTGATTTATCTTGACTCCTATTTTATTGTCTCGTAGACTGATGCCATA
AAGTCAATCTCGATTCTTCC

Downstream 100 bases:

>100_bases
TACTGGGCATAGGTGTAGATTTAGTATGCACGAGAAGGATACAAGCTTTAATTGCGAAGTATGGGAACAAGTTCACCAAT
AGAATATTTTCTGAGAAAGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRLSEFYSPTVKNVSSDVVSASHKYSIRAGIVSQTASGIYTLLPLGLMVLRKVENIIREEINAVGFSEILMPTMQPADLW
KESQRYDSYGQELIRIHDRGGREMVLGPTHEEVVTDLVRSSLKSYRDLPVNLYQIQWKFRDELRPRNGILRSREFLMMDA
YSFDTDFEKAMKTYDAVFRAYRKAFKRMNLQTIALKADMGAIGGSVSHEFHVLTPTGESTVYYDERALELSEMNDYGIEE
LKEVYAATDDMHDEKSCGIAPEDLKTARGIEVGHIFYLDDRYSRTMNVKFCNTDGHSGTHVKMGCYGIGISRLIGALIEV
FHDDAGIKWPLSVAPFKVGIVNLFSKNEECKRVSERIHSVLPNDSLYDDRDDTPGVKLSRMDLIGLPWQVIVGNSFIKDG
VLELKNRATGDIELLSVDDVVSRISV

Sequences:

>Translated_426_residues
MRLSEFYSPTVKNVSSDVVSASHKYSIRAGIVSQTASGIYTLLPLGLMVLRKVENIIREEINAVGFSEILMPTMQPADLW
KESQRYDSYGQELIRIHDRGGREMVLGPTHEEVVTDLVRSSLKSYRDLPVNLYQIQWKFRDELRPRNGILRSREFLMMDA
YSFDTDFEKAMKTYDAVFRAYRKAFKRMNLQTIALKADMGAIGGSVSHEFHVLTPTGESTVYYDERALELSEMNDYGIEE
LKEVYAATDDMHDEKSCGIAPEDLKTARGIEVGHIFYLDDRYSRTMNVKFCNTDGHSGTHVKMGCYGIGISRLIGALIEV
FHDDAGIKWPLSVAPFKVGIVNLFSKNEECKRVSERIHSVLPNDSLYDDRDDTPGVKLSRMDLIGLPWQVIVGNSFIKDG
VLELKNRATGDIELLSVDDVVSRISV
>Mature_426_residues
MRLSEFYSPTVKNVSSDVVSASHKYSIRAGIVSQTASGIYTLLPLGLMVLRKVENIIREEINAVGFSEILMPTMQPADLW
KESQRYDSYGQELIRIHDRGGREMVLGPTHEEVVTDLVRSSLKSYRDLPVNLYQIQWKFRDELRPRNGILRSREFLMMDA
YSFDTDFEKAMKTYDAVFRAYRKAFKRMNLQTIALKADMGAIGGSVSHEFHVLTPTGESTVYYDERALELSEMNDYGIEE
LKEVYAATDDMHDEKSCGIAPEDLKTARGIEVGHIFYLDDRYSRTMNVKFCNTDGHSGTHVKMGCYGIGISRLIGALIEV
FHDDAGIKWPLSVAPFKVGIVNLFSKNEECKRVSERIHSVLPNDSLYDDRDDTPGVKLSRMDLIGLPWQVIVGNSFIKDG
VLELKNRATGDIELLSVDDVVSRISV

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=437, Percent_Identity=34.5537757437071, Blast_Score=269, Evalue=3e-72,
Organism=Escherichia coli, GI1786392, Length=250, Percent_Identity=53.2, Blast_Score=269, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI115532348, Length=385, Percent_Identity=31.6883116883117, Blast_Score=196, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6320931, Length=206, Percent_Identity=37.378640776699, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24656200, Length=408, Percent_Identity=35.0490196078431, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ANAPZ (Q2GK70)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505231.1
- ProteinModelPortal:   Q2GK70
- SMR:   Q2GK70
- STRING:   Q2GK70
- GeneID:   3930859
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0647
- NMPDR:   fig|212042.5.peg.632
- TIGR:   APH_0647
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q2GK70
- ProtClustDB:   PRK12325
- BioCyc:   APHA212042:APH_0647-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 48224; Mature: 48224

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSEFYSPTVKNVSSDVVSASHKYSIRAGIVSQTASGIYTLLPLGLMVLRKVENIIREE
CCCHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAVGFSEILMPTMQPADLWKESQRYDSYGQELIRIHDRGGREMVLGPTHEEVVTDLVRS
HHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
SLKSYRDLPVNLYQIQWKFRDELRPRNGILRSREFLMMDAYSFDTDFEKAMKTYDAVFRA
HHHHHHCCCCEEEEEEEEEHHCCCCCCCCHHCCCEEEEECCCCCCHHHHHHHHHHHHHHH
YRKAFKRMNLQTIALKADMGAIGGSVSHEFHVLTPTGESTVYYDERALELSEMNDYGIEE
HHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEECHHHHHHHHCCCCCHHH
LKEVYAATDDMHDEKSCGIAPEDLKTARGIEVGHIFYLDDRYSRTMNVKFCNTDGHSGTH
HHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCE
VKMGCYGIGISRLIGALIEVFHDDAGIKWPLSVAPFKVGIVNLFSKNEECKRVSERIHSV
EEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LPNDSLYDDRDDTPGVKLSRMDLIGLPWQVIVGNSFIKDGVLELKNRATGDIELLSVDDV
CCCCCCCCCCCCCCCCEEECEEEECCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEHHHH
VSRISV
HHHHCC
>Mature Secondary Structure
MRLSEFYSPTVKNVSSDVVSASHKYSIRAGIVSQTASGIYTLLPLGLMVLRKVENIIREE
CCCHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAVGFSEILMPTMQPADLWKESQRYDSYGQELIRIHDRGGREMVLGPTHEEVVTDLVRS
HHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
SLKSYRDLPVNLYQIQWKFRDELRPRNGILRSREFLMMDAYSFDTDFEKAMKTYDAVFRA
HHHHHHCCCCEEEEEEEEEHHCCCCCCCCHHCCCEEEEECCCCCCHHHHHHHHHHHHHHH
YRKAFKRMNLQTIALKADMGAIGGSVSHEFHVLTPTGESTVYYDERALELSEMNDYGIEE
HHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEECHHHHHHHHCCCCCHHH
LKEVYAATDDMHDEKSCGIAPEDLKTARGIEVGHIFYLDDRYSRTMNVKFCNTDGHSGTH
HHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCE
VKMGCYGIGISRLIGALIEVFHDDAGIKWPLSVAPFKVGIVNLFSKNEECKRVSERIHSV
EEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LPNDSLYDDRDDTPGVKLSRMDLIGLPWQVIVGNSFIKDGVLELKNRATGDIELLSVDDV
CCCCCCCCCCCCCCCCEEECEEEECCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEHHHH
VSRISV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA