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The map label for this gene is ndhF [H]

Identifier: 88606904

GI number: 88606904

Start: 181049

End: 182551

Strand: Reverse

Name: ndhF [H]

Synonym: APH_0181

Alternate gene names: 88606904

Gene position: 182551-181049 (Counterclockwise)

Preceding gene: 88607932

Following gene: 88607613

Centisome position: 12.41

GC content: 43.91

Gene sequence:

>1503_bases
GTGATTGTGGGTTTGCTTTGCCGCTCAGATGTATTGCTGTTTGTTGTGGTGTCGTTACCATTCGTCTGCTCTGCGCTGAT
CATGGCTCTGAAAAGGCTTTCACACTCTCAAGAGGCGCTCTCGACTGTGGCTTCTCTTGTTTTGTTCCCCACAACATGTG
CTTTGTACTTCGCGTTACAGGGCAATCCAGCAATACTACAGTATCGTCTGTCCTCAGCGATTCTTTTCATTCTTGAACCT
GAAGCCCTGGGTATAGTATTCGGGATCTTAGTATCGTTCCTTTGGGCTGTAACTAATATCTACACCATAGGTTACATGAA
CAAGGTTTATGGTAAGCTGCAGGATAGATCAGTTTTTTACTCCTGCTTTGCCGCTAGTATAGGCTGCACCATAGGTATAG
CATTTGCTGGAAACTTGGTGACTCTCTTCATATTTTACGAGATGCTCACCGTATGCACTTATCCTCTAATAATTCATGGT
GGAAGTAAAGACTCAATAGCTTCTGGAAGCTTTTACATAAAGACGCTTTTGTGTTCTTCAGTGTTTTTGTTCCTTCCAGC
AATTGCTATAATATGCGGAATGAGTGATGGTGCTGGATTATTCAGCGCAAAGAGTTTCATTTCTGAGACGCACCCCAGCC
TTCTCCCTTTTATAGTCACTATGCTGTGCTATGGAGTGGCTAAAGCCGCCATCATGCCGGTGCATCTATGGCTACCCAGA
GCAATGGTCGCTCCGACACCTGTTAGCGCGTTGCTGCACGCGGTGGCTGTAGTAAAATCCGGGGTATTTACCTTAATCAA
GATTACTGTATGCGCCATTGGCGTACACGGAATGTATAACTACTATAATGCTCCTGTAGCTGTCGATGTAGCACCTCACC
ACAACATAATGCTGTATCTCTCAGTGGTTACTATCATTGTGGGATCTGTCTTTGCAGTGACTCAGAAAAATCTCAAAAGG
TTACTCGCATACTCTACTATATCGCAGTTATCGTACATAACTTTAGCTTTATCCATGTTCACTGATGGAGCTGTAACAGC
TGCAGTGTTTCAGATGGTGTGCCATGCTTTTGCGAAAATCACATTATTCTTCGCTGTGGGAGCTGTATATGCTGCTTCTG
GTAAAACTACGATAAGGGATATTGATGGTTTAGGCAGAGTTATGCCCATAACTTTCACTGCATTCTGCATTGGGGCGCTT
GCTATGATCGGCATCCCTCCAGCTGCCACATTTTGGGGTAAGTTCTATATTATTACAGAGGCTTTAAAGCAGCAGAACTA
CATTGTAATTTTCACTGTAGCCTTGAGTACCGTGCTTAACACCTTATACTTTATACCTTTGATGTACAGAGTATTCTTTG
CTGCTGACGCAACGTGTGCGGGGAGAGTTAAGGAGGAGGCACCACCCTTAATCTTACTGGCAATGCTCGTATCATCTACG
TGTACGATTTTACTGTTCTTAAAACCCGACATCGTACTAGGAATGCTATCTGTATTCGGTTAA

Upstream 100 bases:

>100_bases
CTTTGGAAAACATAGATGCATGCCTTTGGTGTAGGTAAAGCAAAGTGTTTTACTTATAGATATTTTGTTGCTATTAATGT
CGCTTAACTTTATAGATTGA

Downstream 100 bases:

>100_bases
TTATGATGTGAGTCACGTGCATGGGTTAAGTTTTTTAATGCGTCCTTCTGATGTGGAAAGTCAGTGCATTGCCCTACTAC
TGCAAATTATACAATGAGGT

Product: NADH-ubiquinone/plastoquinone oxidoreductase family protein

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MIVGLLCRSDVLLFVVVSLPFVCSALIMALKRLSHSQEALSTVASLVLFPTTCALYFALQGNPAILQYRLSSAILFILEP
EALGIVFGILVSFLWAVTNIYTIGYMNKVYGKLQDRSVFYSCFAASIGCTIGIAFAGNLVTLFIFYEMLTVCTYPLIIHG
GSKDSIASGSFYIKTLLCSSVFLFLPAIAIICGMSDGAGLFSAKSFISETHPSLLPFIVTMLCYGVAKAAIMPVHLWLPR
AMVAPTPVSALLHAVAVVKSGVFTLIKITVCAIGVHGMYNYYNAPVAVDVAPHHNIMLYLSVVTIIVGSVFAVTQKNLKR
LLAYSTISQLSYITLALSMFTDGAVTAAVFQMVCHAFAKITLFFAVGAVYAASGKTTIRDIDGLGRVMPITFTAFCIGAL
AMIGIPPAATFWGKFYIITEALKQQNYIVIFTVALSTVLNTLYFIPLMYRVFFAADATCAGRVKEEAPPLILLAMLVSST
CTILLFLKPDIVLGMLSVFG

Sequences:

>Translated_500_residues
MIVGLLCRSDVLLFVVVSLPFVCSALIMALKRLSHSQEALSTVASLVLFPTTCALYFALQGNPAILQYRLSSAILFILEP
EALGIVFGILVSFLWAVTNIYTIGYMNKVYGKLQDRSVFYSCFAASIGCTIGIAFAGNLVTLFIFYEMLTVCTYPLIIHG
GSKDSIASGSFYIKTLLCSSVFLFLPAIAIICGMSDGAGLFSAKSFISETHPSLLPFIVTMLCYGVAKAAIMPVHLWLPR
AMVAPTPVSALLHAVAVVKSGVFTLIKITVCAIGVHGMYNYYNAPVAVDVAPHHNIMLYLSVVTIIVGSVFAVTQKNLKR
LLAYSTISQLSYITLALSMFTDGAVTAAVFQMVCHAFAKITLFFAVGAVYAASGKTTIRDIDGLGRVMPITFTAFCIGAL
AMIGIPPAATFWGKFYIITEALKQQNYIVIFTVALSTVLNTLYFIPLMYRVFFAADATCAGRVKEEAPPLILLAMLVSST
CTILLFLKPDIVLGMLSVFG
>Mature_500_residues
MIVGLLCRSDVLLFVVVSLPFVCSALIMALKRLSHSQEALSTVASLVLFPTTCALYFALQGNPAILQYRLSSAILFILEP
EALGIVFGILVSFLWAVTNIYTIGYMNKVYGKLQDRSVFYSCFAASIGCTIGIAFAGNLVTLFIFYEMLTVCTYPLIIHG
GSKDSIASGSFYIKTLLCSSVFLFLPAIAIICGMSDGAGLFSAKSFISETHPSLLPFIVTMLCYGVAKAAIMPVHLWLPR
AMVAPTPVSALLHAVAVVKSGVFTLIKITVCAIGVHGMYNYYNAPVAVDVAPHHNIMLYLSVVTIIVGSVFAVTQKNLKR
LLAYSTISQLSYITLALSMFTDGAVTAAVFQMVCHAFAKITLFFAVGAVYAASGKTTIRDIDGLGRVMPITFTAFCIGAL
AMIGIPPAATFWGKFYIITEALKQQNYIVIFTVALSTVLNTLYFIPLMYRVFFAADATCAGRVKEEAPPLILLAMLVSST
CTILLFLKPDIVLGMLSVFG

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=424, Percent_Identity=27.8301886792453, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1788829, Length=364, Percent_Identity=29.3956043956044, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788827, Length=423, Percent_Identity=24.5862884160757, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788614, Length=399, Percent_Identity=27.3182957393484, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788831, Length=425, Percent_Identity=25.8823529411765, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788613, Length=339, Percent_Identity=26.2536873156342, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI2367154, Length=209, Percent_Identity=26.7942583732057, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693160, Length=347, Percent_Identity=24.4956772334294, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 53922; Mature: 53922

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVGLLCRSDVLLFVVVSLPFVCSALIMALKRLSHSQEALSTVASLVLFPTTCALYFALQ
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEC
GNPAILQYRLSSAILFILEPEALGIVFGILVSFLWAVTNIYTIGYMNKVYGKLQDRSVFY
CCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SCFAASIGCTIGIAFAGNLVTLFIFYEMLTVCTYPLIIHGGSKDSIASGSFYIKTLLCSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
VFLFLPAIAIICGMSDGAGLFSAKSFISETHPSLLPFIVTMLCYGVAKAAIMPVHLWLPR
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AMVAPTPVSALLHAVAVVKSGVFTLIKITVCAIGVHGMYNYYNAPVAVDVAPHHNIMLYL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
SVVTIIVGSVFAVTQKNLKRLLAYSTISQLSYITLALSMFTDGAVTAAVFQMVCHAFAKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLFFAVGAVYAASGKTTIRDIDGLGRVMPITFTAFCIGALAMIGIPPAATFWGKFYIITE
HHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ALKQQNYIVIFTVALSTVLNTLYFIPLMYRVFFAADATCAGRVKEEAPPLILLAMLVSST
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCC
CTILLFLKPDIVLGMLSVFG
CEEEEEECHHHHHHHHHHCC
>Mature Secondary Structure
MIVGLLCRSDVLLFVVVSLPFVCSALIMALKRLSHSQEALSTVASLVLFPTTCALYFALQ
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEC
GNPAILQYRLSSAILFILEPEALGIVFGILVSFLWAVTNIYTIGYMNKVYGKLQDRSVFY
CCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SCFAASIGCTIGIAFAGNLVTLFIFYEMLTVCTYPLIIHGGSKDSIASGSFYIKTLLCSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
VFLFLPAIAIICGMSDGAGLFSAKSFISETHPSLLPFIVTMLCYGVAKAAIMPVHLWLPR
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AMVAPTPVSALLHAVAVVKSGVFTLIKITVCAIGVHGMYNYYNAPVAVDVAPHHNIMLYL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
SVVTIIVGSVFAVTQKNLKRLLAYSTISQLSYITLALSMFTDGAVTAAVFQMVCHAFAKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLFFAVGAVYAASGKTTIRDIDGLGRVMPITFTAFCIGALAMIGIPPAATFWGKFYIITE
HHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ALKQQNYIVIFTVALSTVLNTLYFIPLMYRVFFAADATCAGRVKEEAPPLILLAMLVSST
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCC
CTILLFLKPDIVLGMLSVFG
CEEEEEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]