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The map label for this gene is purA [H]

Identifier: 88606829

GI number: 88606829

Start: 318534

End: 319838

Strand: Reverse

Name: purA [H]

Synonym: APH_0336

Alternate gene names: 88606829

Gene position: 319838-318534 (Counterclockwise)

Preceding gene: 88607299

Following gene: 88607032

Centisome position: 21.74

GC content: 46.05

Gene sequence:

>1305_bases
ATGCCAAGTATAGTAGTAGTTGGGCTACAGTGGGGTGATGAAGGAAAAGGCAAAATAGTAGACTGGCTATCCGAAAATGC
CGATACCGTAGTCAGGTTTCAGGGTGGAAGCAATGCTGGTCATACCATAGTAATAGAAGGTAAGGTCTATAAGCTGAGTT
TGCTGCCGTCATCCGTGTTGCGACCTGGTAAGCAGTCCATAATCGGAAATGGGGTAGTGCTTGATCCTTATGCTCTTGTA
AGAGAGATTGAAAAGTTGCAAGAAAGCAATATTGAGTTGACTCCAGAAAACTTGTCTCTATCTGAGACATGTCCGCTTGT
GCTCAGCGTACATCGGGAAACCGAAGTGATACTCGAGAATTTGCGTGGTGTGCACGCGATAGGCACAACTTGCATGGGTA
TCGGGCCTTGCTATGAGGACAAAATAGGCCGTCGTGCAATAAGACTTTGCGATTTGTTAGATGAGAAATCGCTTCGTGAG
AAAGTAGAGTTCCTGCTCTCCTACCATAATCTGCTCAGAAAAGCCACAAATAAGGATGAATTGCAGAGCGAAAAGGTTAT
GGAGGAAATACTGTTCATAGCCCCTAAGGTATTGCCGTTTATGAAGCACGTAGGGGGTATGATTTACGAACAACTCGCCG
AAGGTAAGACTGTTCTGTTTGAAGGTGCACAAGGCGCATTGCTTGATATAGACCACGGAACGTATCCGTATGTAACTTCA
AGTAATACTCTTGCTGGCTGTGTTCCAATCGGTTGCGGAGTTGGCTCTCCTGGCAGTATTCGTGTACTTGGACTGGCTAA
GGCATATACTACCAGAGTTGGCAATGGCCCCTTCATAACTGAACTCACTGGAGATTTGGACGATTTAATGGTCGAAAGAG
GCAGAGAGTTTGGTACTGTGAGCAAGCGGAGACGTAGGTGCGGCTGGTTTGATGCAGCCCTAGTACGTCATGCAGCGTTG
TTATCAGGCGCTACTGAGCTTGCTTTGACGAAGTTAGATGTTTTGGACACATTGAGTGAAATAAAGATATGTGTGGGCTA
TAAACATGGTGAGTCATTTTACAATTATCTGCCACCTGCTTCTCACATACAAGGCGCTTTGGAGCCAATATATGAGGTCT
TACCCGGATGGCAGAGCAGCACTCTAGGCGCTGTGTCCAAGAACGATCTTCCTGCCAATGCGTTGGCGTATGTTCAAAGA
ATCGAAGAACTTGTGCAAATCCCTGTCAGCCTGGTATCTACCAGTCCCGACAGAGATCACATAGTTTTCTTGGGAGATAG
CTCATCTATCGCTAAGTCATACTAG

Upstream 100 bases:

>100_bases
ACTCTCGACAAAATACCTAAGAGGTATGCTTCTCTCCGATCAGTGTTGACACTATTTCTTTATTTTGCTAGCTTGACCTG
GTGTTTTTTACGGACGTTCG

Downstream 100 bases:

>100_bases
AGAGATTGTGTCACTGCTTAGCTAAGTAGAGGACTGCTTCTTTTGTTGTGCTGTTTGGTACATATTAGTAAAGCATGTCT
CTATGTTGGTAACGTATCAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPSIVVVGLQWGDEGKGKIVDWLSENADTVVRFQGGSNAGHTIVIEGKVYKLSLLPSSVLRPGKQSIIGNGVVLDPYALV
REIEKLQESNIELTPENLSLSETCPLVLSVHRETEVILENLRGVHAIGTTCMGIGPCYEDKIGRRAIRLCDLLDEKSLRE
KVEFLLSYHNLLRKATNKDELQSEKVMEEILFIAPKVLPFMKHVGGMIYEQLAEGKTVLFEGAQGALLDIDHGTYPYVTS
SNTLAGCVPIGCGVGSPGSIRVLGLAKAYTTRVGNGPFITELTGDLDDLMVERGREFGTVSKRRRRCGWFDAALVRHAAL
LSGATELALTKLDVLDTLSEIKICVGYKHGESFYNYLPPASHIQGALEPIYEVLPGWQSSTLGAVSKNDLPANALAYVQR
IEELVQIPVSLVSTSPDRDHIVFLGDSSSIAKSY

Sequences:

>Translated_434_residues
MPSIVVVGLQWGDEGKGKIVDWLSENADTVVRFQGGSNAGHTIVIEGKVYKLSLLPSSVLRPGKQSIIGNGVVLDPYALV
REIEKLQESNIELTPENLSLSETCPLVLSVHRETEVILENLRGVHAIGTTCMGIGPCYEDKIGRRAIRLCDLLDEKSLRE
KVEFLLSYHNLLRKATNKDELQSEKVMEEILFIAPKVLPFMKHVGGMIYEQLAEGKTVLFEGAQGALLDIDHGTYPYVTS
SNTLAGCVPIGCGVGSPGSIRVLGLAKAYTTRVGNGPFITELTGDLDDLMVERGREFGTVSKRRRRCGWFDAALVRHAAL
LSGATELALTKLDVLDTLSEIKICVGYKHGESFYNYLPPASHIQGALEPIYEVLPGWQSSTLGAVSKNDLPANALAYVQR
IEELVQIPVSLVSTSPDRDHIVFLGDSSSIAKSY
>Mature_433_residues
PSIVVVGLQWGDEGKGKIVDWLSENADTVVRFQGGSNAGHTIVIEGKVYKLSLLPSSVLRPGKQSIIGNGVVLDPYALVR
EIEKLQESNIELTPENLSLSETCPLVLSVHRETEVILENLRGVHAIGTTCMGIGPCYEDKIGRRAIRLCDLLDEKSLREK
VEFLLSYHNLLRKATNKDELQSEKVMEEILFIAPKVLPFMKHVGGMIYEQLAEGKTVLFEGAQGALLDIDHGTYPYVTSS
NTLAGCVPIGCGVGSPGSIRVLGLAKAYTTRVGNGPFITELTGDLDDLMVERGREFGTVSKRRRRCGWFDAALVRHAALL
SGATELALTKLDVLDTLSEIKICVGYKHGESFYNYLPPASHIQGALEPIYEVLPGWQSSTLGAVSKNDLPANALAYVQRI
EELVQIPVSLVSTSPDRDHIVFLGDSSSIAKSY

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=39.8126463700234, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=40.0468384074941, Blast_Score=298, Evalue=5e-81,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=38.1909547738693, Blast_Score=258, Evalue=1e-68,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=47.4178403755869, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=40.1869158878505, Blast_Score=290, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=40.1869158878505, Blast_Score=290, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6324109, Length=426, Percent_Identity=38.4976525821596, Blast_Score=282, Evalue=6e-77,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=42.5178147268409, Blast_Score=311, Evalue=6e-85,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47332; Mature: 47201

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2 ; PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIVVVGLQWGDEGKGKIVDWLSENADTVVRFQGGSNAGHTIVIEGKVYKLSLLPSSVL
CCCEEEEEEEECCCCCCCCHHHHCCCCCEEEEEECCCCCCCEEEEECEEEEEEECCHHHH
RPGKQSIIGNGVVLDPYALVREIEKLQESNIELTPENLSLSETCPLVLSVHRETEVILEN
CCCCHHHCCCCEEECHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEECHHHHHHHHH
LRGVHAIGTTCMGIGPCYEDKIGRRAIRLCDLLDEKSLREKVEFLLSYHNLLRKATNKDE
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQSEKVMEEILFIAPKVLPFMKHVGGMIYEQLAEGKTVLFEGAQGALLDIDHGTYPYVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTLAGCVPIGCGVGSPGSIRVLGLAKAYTTRVGNGPFITELTGDLDDLMVERGREFGTV
CCCEEEEEEECCCCCCCCCEEEEEEHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCCCH
SKRRRRCGWFDAALVRHAALLSGATELALTKLDVLDTLSEIKICVGYKHGESFYNYLPPA
HHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHCCCCH
SHIQGALEPIYEVLPGWQSSTLGAVSKNDLPANALAYVQRIEELVQIPVSLVSTSPDRDH
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCE
IVFLGDSSSIAKSY
EEEEECCCHHHCCC
>Mature Secondary Structure 
PSIVVVGLQWGDEGKGKIVDWLSENADTVVRFQGGSNAGHTIVIEGKVYKLSLLPSSVL
CCEEEEEEEECCCCCCCCHHHHCCCCCEEEEEECCCCCCCEEEEECEEEEEEECCHHHH
RPGKQSIIGNGVVLDPYALVREIEKLQESNIELTPENLSLSETCPLVLSVHRETEVILEN
CCCCHHHCCCCEEECHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEECHHHHHHHHH
LRGVHAIGTTCMGIGPCYEDKIGRRAIRLCDLLDEKSLREKVEFLLSYHNLLRKATNKDE
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQSEKVMEEILFIAPKVLPFMKHVGGMIYEQLAEGKTVLFEGAQGALLDIDHGTYPYVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTLAGCVPIGCGVGSPGSIRVLGLAKAYTTRVGNGPFITELTGDLDDLMVERGREFGTV
CCCEEEEEEECCCCCCCCCEEEEEEHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCCCH
SKRRRRCGWFDAALVRHAALLSGATELALTKLDVLDTLSEIKICVGYKHGESFYNYLPPA
HHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHCCCCH
SHIQGALEPIYEVLPGWQSSTLGAVSKNDLPANALAYVQRIEELVQIPVSLVSTSPDRDH
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCE
IVFLGDSSSIAKSY
EEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA