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The map label for this gene is rpoA

Identifier: 88606699

GI number: 88606699

Start: 286531

End: 287565

Strand: Direct

Name: rpoA

Synonym: APH_0303

Alternate gene names: 88606699

Gene position: 286531-287565 (Clockwise)

Preceding gene: 88607425

Following gene: 88606692

Centisome position: 19.47

GC content: 44.44

Gene sequence:

>1035_bases
ATGGCTGACCATTGGAACAAGTTGACCAGGCCATCTTCTATCAAGGTTGTAGGTGGTAAGGAGCCTTGTGTTATGGAGTT
GGTGATTGAGCCTTTGGAGAGTGGTTTCGCGCTTACTCTAGGCAATGCTCTGAGGCGTGTTATGATGTCATCTCTCAGGG
GTTTTGCCGTCTACGGGATAGAGATTGAAGGAGCTTCGCATGAGCTTACTGCTCTTTCGGGAGTGAGAGAGGATGTTGCG
GATTTGGTACTGAACCTCAGTATGCTTAGAGTGAAGTTATTGAACTCGAATCAAAGGGTTCTGAGGCTTGTTGCTCGGGG
TCCGGGAGAGGTAACTGCTGCTTCGATAGTTGATTCTGCGGATCACGTAGTTTTGAATAAGGATCTGCATATTTGTACGC
TGGGTAAGGATGTGGATTTTTGCATGAAGATCTATGTCAACAGCGGTAAGGGTTATGTTCCGGCTACGGAGTATAGGGCT
GCGTCAAGGTCTGGTGGTGCTTCCGAAGTTGGTTCTGGATTTATAGCTACTAATGCGCTTTATAGTCCTGTAAAGAAGGT
GGCTTTAAAAATAGAGAGCAGTAGGATAGGGCAGTTTACTGATTACGACAGGCTTATGTTGACGGTTGAGACAGATGGCT
CTGTTGCTCCTGATGATGCTGTTGCGGTTGCTGCGAAGATATTGCAAGATCAGCTGCAGTCATTCATAAGTTTTGATGAA
GTGGAAGAGACTAGGAAGAGTGTGGATAAGGAAGAGGGTGTACTTCCTTATGACCATAATCTCCTTAGGAAGGTTGATGA
ACTGGAGCTTTCTGTTAGATCGCATAACTGTTTGAAGAACGATAATATCACTTATATAGGTGATCTTGTTCAGAGAACGG
AGTCTGATATGTTGAGAACTCCGAATTTTGGTAGAAAATCTCTTAATGAGATAAATGAGGTCTTGGCTAGTATGAATCTG
CATTTGGGGATGAAGGTGCCGAATTGGCCGCCGGAGTCTATAGAGAATTTGAGTAAGCAGTATAGTGAAGATTAA

Upstream 100 bases:

>100_bases
TTGCCAAAAAGGCGCAGGGTGTAGTTTTTATTGAGAGAGATTTATGTCAATTTCTAACGGCGATGGGTCTAGTAGTGCGT
GCTATGGGGGTGGTTTTTCC

Downstream 100 bases:

>100_bases
TAATTGGTTTGTTTGCGCTGATTGGGGGTTAGAATGAGGCATGGTGTAAGTTATAGGAAGTTTTCTCGTCCTACGGCGCA
TAGAATGGCTATGATGATGA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MADHWNKLTRPSSIKVVGGKEPCVMELVIEPLESGFALTLGNALRRVMMSSLRGFAVYGIEIEGASHELTALSGVREDVA
DLVLNLSMLRVKLLNSNQRVLRLVARGPGEVTAASIVDSADHVVLNKDLHICTLGKDVDFCMKIYVNSGKGYVPATEYRA
ASRSGGASEVGSGFIATNALYSPVKKVALKIESSRIGQFTDYDRLMLTVETDGSVAPDDAVAVAAKILQDQLQSFISFDE
VEETRKSVDKEEGVLPYDHNLLRKVDELELSVRSHNCLKNDNITYIGDLVQRTESDMLRTPNFGRKSLNEINEVLASMNL
HLGMKVPNWPPESIENLSKQYSED

Sequences:

>Translated_344_residues
MADHWNKLTRPSSIKVVGGKEPCVMELVIEPLESGFALTLGNALRRVMMSSLRGFAVYGIEIEGASHELTALSGVREDVA
DLVLNLSMLRVKLLNSNQRVLRLVARGPGEVTAASIVDSADHVVLNKDLHICTLGKDVDFCMKIYVNSGKGYVPATEYRA
ASRSGGASEVGSGFIATNALYSPVKKVALKIESSRIGQFTDYDRLMLTVETDGSVAPDDAVAVAAKILQDQLQSFISFDE
VEETRKSVDKEEGVLPYDHNLLRKVDELELSVRSHNCLKNDNITYIGDLVQRTESDMLRTPNFGRKSLNEINEVLASMNL
HLGMKVPNWPPESIENLSKQYSED
>Mature_343_residues
ADHWNKLTRPSSIKVVGGKEPCVMELVIEPLESGFALTLGNALRRVMMSSLRGFAVYGIEIEGASHELTALSGVREDVAD
LVLNLSMLRVKLLNSNQRVLRLVARGPGEVTAASIVDSADHVVLNKDLHICTLGKDVDFCMKIYVNSGKGYVPATEYRAA
SRSGGASEVGSGFIATNALYSPVKKVALKIESSRIGQFTDYDRLMLTVETDGSVAPDDAVAVAAKILQDQLQSFISFDEV
EETRKSVDKEEGVLPYDHNLLRKVDELELSVRSHNCLKNDNITYIGDLVQRTESDMLRTPNFGRKSLNEINEVLASMNLH
LGMKVPNWPPESIENLSKQYSED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=310, Percent_Identity=44.5161290322581, Blast_Score=257, Evalue=8e-70,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_ANAPZ (Q2GL36)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504915.1
- ProteinModelPortal:   Q2GL36
- STRING:   Q2GL36
- GeneID:   3929972
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0303
- NMPDR:   fig|212042.5.peg.287
- TIGR:   APH_0303
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q2GL36
- ProtClustDB:   PRK05182
- BioCyc:   APHA212042:APH_0303-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37857; Mature: 37725

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADHWNKLTRPSSIKVVGGKEPCVMELVIEPLESGFALTLGNALRRVMMSSLRGFAVYGI
CCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCEEEEEE
EIEGASHELTALSGVREDVADLVLNLSMLRVKLLNSNQRVLRLVARGPGEVTAASIVDSA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCC
DHVVLNKDLHICTLGKDVDFCMKIYVNSGKGYVPATEYRAASRSGGASEVGSGFIATNAL
CCEEEECCEEEEEECCCHHEEEEEEEECCCCCCCCHHHHHHCCCCCHHHHCCCHHHHHHH
YSPVKKVALKIESSRIGQFTDYDRLMLTVETDGSVAPDDAVAVAAKILQDQLQSFISFDE
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VEETRKSVDKEEGVLPYDHNLLRKVDELELSVRSHNCLKNDNITYIGDLVQRTESDMLRT
HHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
PNFGRKSLNEINEVLASMNLHLGMKVPNWPPESIENLSKQYSED
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADHWNKLTRPSSIKVVGGKEPCVMELVIEPLESGFALTLGNALRRVMMSSLRGFAVYGI
CCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCEEEEEE
EIEGASHELTALSGVREDVADLVLNLSMLRVKLLNSNQRVLRLVARGPGEVTAASIVDSA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCC
DHVVLNKDLHICTLGKDVDFCMKIYVNSGKGYVPATEYRAASRSGGASEVGSGFIATNAL
CCEEEECCEEEEEECCCHHEEEEEEEECCCCCCCCHHHHHHCCCCCHHHHCCCHHHHHHH
YSPVKKVALKIESSRIGQFTDYDRLMLTVETDGSVAPDDAVAVAAKILQDQLQSFISFDE
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VEETRKSVDKEEGVLPYDHNLLRKVDELELSVRSHNCLKNDNITYIGDLVQRTESDMLRT
HHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
PNFGRKSLNEINEVLASMNLHLGMKVPNWPPESIENLSKQYSED
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA