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The map label for this gene is purU

Identifier: 87200875

GI number: 87200875

Start: 3085570

End: 3086424

Strand: Direct

Name: purU

Synonym: Saro_2862

Alternate gene names: 87200875

Gene position: 3085570-3086424 (Clockwise)

Preceding gene: 87200871

Following gene: 87200876

Centisome position: 86.63

GC content: 63.04

Gene sequence:

>855_bases
ATGAGCGCATCGCTGATTTTGACGCTGTCGTGCGAGGACAAGCCGGGCATCGTCGCCCGGGTCACGGGCAACCTGTTCGA
GGCGGGTGGCAACATTCGCGAGGCGCAGCAATTCGACGATCCGCTGAGCGGCCGTTTCTTCATGCGCGTGGTGTTCGATC
CGGCCGAAGGTTCGGTCGAGCATTACCGCGCGGCCTTCGCGGCGGTGGCCGACCACTATGCAATGGAATGGAACCTGCGC
GACACGACCGTGAAGCGGAAGGTCATCCTGATGGTCAGCAAGTTCGACCACTGCCTTGGCGACTTGCTCTACCGCACGCG
AATCGGCGAACTGCCGATGGACGTCGTGGCGATCCTCGGCAACCATCCCAAGGAAGCGCTCAACATCTCGCTGATCGGCG
ACATTCCCTACCATCACCTGCCCATCACCAAGGACACCAAGCCACAGCAGGAAGCCGAAGTGAAGCGCATCGTCACCGAG
ACGGGCGCGGAACTGGTTGTACTGGCGCGCTACATGCAGATCCTTTCGGACGACCTCGCCGCGTTCCTTTCGGGCCGCTG
CATCAACATCCACCACTCGTTCCTGCCGAGCTTCAAGGGCGCCAAGCCCTATCACCAGGCGCATGCCCGCGGCGTGAAGA
TGATCGGCGCGACCGGCCACTACGTGACCGCCGACCTCGACGAAGGCCCGATCATCCACCAGGACGTGGAAACGGTGACC
CACGCCGACAGCCCGGATGACCTGGTACGCAAGGGCCGCGACGTTGAGCGCCGCGTGCTGGCCGAAGCGGTGCGCCTGCA
TCTTGAAGACCGCGCACTGGTCAACGGCAACAAGACCGTCGTGTTCAAGAGCTGA

Upstream 100 bases:

>100_bases
GCAGGTTGAAAAATTTGTATGCAACACATACAAGTTTGGAAGAGATTCGCAATGGCAAATGTTGCGCCCTATGAGGCGCG
GCCCGGAGGGAGAGATTTTG

Downstream 100 bases:

>100_bases
ACGGTTTGAACAGTTGAGATCGGGCGGCGGCGGGAGCACCATGCTTTCGCCGCCGCTCTTGTTTGCGGGAGAGGACAGAC
ATGCACGTCAACGCACTGGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSASLILTLSCEDKPGIVARVTGNLFEAGGNIREAQQFDDPLSGRFFMRVVFDPAEGSVEHYRAAFAAVADHYAMEWNLR
DTTVKRKVILMVSKFDHCLGDLLYRTRIGELPMDVVAILGNHPKEALNISLIGDIPYHHLPITKDTKPQQEAEVKRIVTE
TGAELVVLARYMQILSDDLAAFLSGRCINIHHSFLPSFKGAKPYHQAHARGVKMIGATGHYVTADLDEGPIIHQDVETVT
HADSPDDLVRKGRDVERRVLAEAVRLHLEDRALVNGNKTVVFKS

Sequences:

>Translated_284_residues
MSASLILTLSCEDKPGIVARVTGNLFEAGGNIREAQQFDDPLSGRFFMRVVFDPAEGSVEHYRAAFAAVADHYAMEWNLR
DTTVKRKVILMVSKFDHCLGDLLYRTRIGELPMDVVAILGNHPKEALNISLIGDIPYHHLPITKDTKPQQEAEVKRIVTE
TGAELVVLARYMQILSDDLAAFLSGRCINIHHSFLPSFKGAKPYHQAHARGVKMIGATGHYVTADLDEGPIIHQDVETVT
HADSPDDLVRKGRDVERRVLAEAVRLHLEDRALVNGNKTVVFKS
>Mature_283_residues
SASLILTLSCEDKPGIVARVTGNLFEAGGNIREAQQFDDPLSGRFFMRVVFDPAEGSVEHYRAAFAAVADHYAMEWNLRD
TTVKRKVILMVSKFDHCLGDLLYRTRIGELPMDVVAILGNHPKEALNISLIGDIPYHHLPITKDTKPQQEAEVKRIVTET
GAELVVLARYMQILSDDLAAFLSGRCINIHHSFLPSFKGAKPYHQAHARGVKMIGATGHYVTADLDEGPIIHQDVETVTH
ADSPDDLVRKGRDVERRVLAEAVRLHLEDRALVNGNKTVVFKS

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=116, Percent_Identity=33.6206896551724, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI209869995, Length=116, Percent_Identity=33.6206896551724, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI209869993, Length=116, Percent_Identity=33.6206896551724, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787483, Length=281, Percent_Identity=39.1459074733096, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1788846, Length=115, Percent_Identity=33.0434782608696, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=173, Percent_Identity=32.3699421965318, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31570; Mature: 31438

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASLILTLSCEDKPGIVARVTGNLFEAGGNIREAQQFDDPLSGRFFMRVVFDPAEGSVE
CCCEEEEEEECCCCCCEEEEEECCHHHCCCCCHHHHHCCCCCCCEEEEEEEEECCCCCHH
HYRAAFAAVADHYAMEWNLRDTTVKRKVILMVSKFDHCLGDLLYRTRIGELPMDVVAILG
HHHHHHHHHHHHHEEEECCCHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
NHPKEALNISLIGDIPYHHLPITKDTKPQQEAEVKRIVTETGAELVVLARYMQILSDDLA
CCCHHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AFLSGRCINIHHSFLPSFKGAKPYHQAHARGVKMIGATGHYVTADLDEGPIIHQDVETVT
HHHCCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECCCCEEEEECCCCCEEHHHHHHHH
HADSPDDLVRKGRDVERRVLAEAVRLHLEDRALVNGNKTVVFKS
CCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEEEC
>Mature Secondary Structure 
SASLILTLSCEDKPGIVARVTGNLFEAGGNIREAQQFDDPLSGRFFMRVVFDPAEGSVE
CCEEEEEEECCCCCCEEEEEECCHHHCCCCCHHHHHCCCCCCCEEEEEEEEECCCCCHH
HYRAAFAAVADHYAMEWNLRDTTVKRKVILMVSKFDHCLGDLLYRTRIGELPMDVVAILG
HHHHHHHHHHHHHEEEECCCHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
NHPKEALNISLIGDIPYHHLPITKDTKPQQEAEVKRIVTETGAELVVLARYMQILSDDLA
CCCHHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AFLSGRCINIHHSFLPSFKGAKPYHQAHARGVKMIGATGHYVTADLDEGPIIHQDVETVT
HHHCCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECCCCEEEEECCCCCEEHHHHHHHH
HADSPDDLVRKGRDVERRVLAEAVRLHLEDRALVNGNKTVVFKS
CCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]