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The map label for this gene is alsS [H]

Identifier: 87200392

GI number: 87200392

Start: 2531046

End: 2532737

Strand: Reverse

Name: alsS [H]

Synonym: Saro_2379

Alternate gene names: 87200392

Gene position: 2532737-2531046 (Counterclockwise)

Preceding gene: 87200393

Following gene: 87200391

Centisome position: 71.11

GC content: 61.23

Gene sequence:

>1692_bases
GTGGCCGCGCGTCTTCCACAGTGGGAGCGACGCGAAATGGCCGACGGCAAGAAGGCATCCGACCTGTTTATCGAGTGCCT
CGAGGAAGAGGGCTGCGAGTACATCTTCGGGGTTCCGGGCGAAGAAAACCTCGACTTCCTCGATTCGCTGTCCCGCTCGA
AGAAGATCAGGCTCGTTCTCACCAGGCATGAACAGGGCGCGGGCTTCATGGCCGCGACTTACGGTCGCCATACCGGCAAG
ACCGGCGTTTGCATCGCGACGCTGGGGCCCGGCGCAACCAACTTCGTCACCGCTGCCGCTTATGCCACGCTGGGCGGGAT
GCCGATGCTCATGATTACCGGGCAGAAGCCGATCAAGAAGTCGAAGCAGGGCCGCTTCCAGATCCTCGATGTCGTCTCGA
TGATGGGGCCGATCACCAAGTTCACGCACCAGATGGCGTCTTCGGACAATATCCCCAGCCGCGTGCGCGAGGCCTATCGC
CTTGCCGAGGAGGAAAAGCCCGGCGCGACCCACATCGAATTGCCCGAGGACATCGCCGACGAACACACTACATCGGTTCC
CCTGAAGCGCAGTCTGGTTCGCCGGCCCAACGCCGATGCCAAGTCGGTCGCACAGGCGGTCCACGCCTTGCAGAACGCCA
AGGCGCCAGTCCTCGTGATCGGCGCCGGGGCGAACCGCAAGATGACTGGCAAGATGCTGCTCGAATTCGTCGAGAAGACC
GGTATCCCGTTCCTGACGACGCAACTCGGCAAGGGCGTGATCGATGAACGCCATCCAAAGTTCCTCGGCTGCGCCGCGCT
TTCGTCCGGCGATTTCGTCCACCGCGCGGTGGAGGATGCCGACATCATCATCAATGTCGGCCACGACGTTATCGAAAAGC
CGCCGTTCTTCATGCGCGAGGGCGGGACGCCGGTCATCCATGTCTCGACCAAGACCGCCGAAGTCGATCCCGTGTACTTC
CCCTCGATCGAAGTGATCGGCGACATCGCCAACGCGATCTGGCAGATGAAGGAAGCGATCACGCCCAATCCCGCGTGGAA
CTTCGACCACATGCTGGCCTATCGCGCGGCCGAAGTTGCGCACACCGCGCCGTTGGCCGCTGACATGCGCTTTCCGGTAT
TCCCGCCGCATCTTGTCCAGCAGGTGCGCGATTGCATGCCCGAGGACGGCATCATCTGCCTCGACAACGGCGTCTACAAG
ATATGGTTCGCGCGCGGCTACACGGCTTACAAGCCCAATACCGTCCTGCTCGACAATGCGCTGGCGACGATGGGGGCGGG
GCTTCCTTCGGCCATGATGAGCGCGATGCTCTATCCCGACCGCAAAGTCATGGCGATCTGCGGCGACGGCGGTTTCATGA
TGAACAGCCAGGAGATGGAGACTGCGGTCCGCCTGGGTCTCAATCTCACGGTGCTGATCCTCAACGACAGCGCCTATGGC
ATGATCCGCTGGAAGCAGGCAAACATGGGATTCGAGGACTTCGGGCTGACGTACAACAACCCCGATTTCGTGAAGTATGC
CGACAGCTACGGGGCCAAGGGATACCGTGTCGAAAGCGCGGAACATCTCGAGAAACTCCTCGCACATTGCCGCGACACTC
CCGGCGTCCACCTGATCGATTGCCCGGTCGACTATTCGGAAAACGACCAGATCCTGAACAAGGACATCAAGGAACTGTCG
AAGGCGCTCTAG

Upstream 100 bases:

>100_bases
GATAGCCCTTGCGCCAAGGGCACGTGCGGGGGCCATAACGACACGCACGAACGGCTTTTCGCCCACGCGGTCGCATCCGG
TAGATGGAGGAAGTCGCAGG

Downstream 100 bases:

>100_bases
GGCTCCTTGATCCGACCGGCATCCCCGCCCCGGCCCCCGGACCGGGGCGGGAACGACGTGGAGACATTGTGAAGTGACCA
AGCTGAAAGACGTCTACCCG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MAARLPQWERREMADGKKASDLFIECLEEEGCEYIFGVPGEENLDFLDSLSRSKKIRLVLTRHEQGAGFMAATYGRHTGK
TGVCIATLGPGATNFVTAAAYATLGGMPMLMITGQKPIKKSKQGRFQILDVVSMMGPITKFTHQMASSDNIPSRVREAYR
LAEEEKPGATHIELPEDIADEHTTSVPLKRSLVRRPNADAKSVAQAVHALQNAKAPVLVIGAGANRKMTGKMLLEFVEKT
GIPFLTTQLGKGVIDERHPKFLGCAALSSGDFVHRAVEDADIIINVGHDVIEKPPFFMREGGTPVIHVSTKTAEVDPVYF
PSIEVIGDIANAIWQMKEAITPNPAWNFDHMLAYRAAEVAHTAPLAADMRFPVFPPHLVQQVRDCMPEDGIICLDNGVYK
IWFARGYTAYKPNTVLLDNALATMGAGLPSAMMSAMLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDSAYG
MIRWKQANMGFEDFGLTYNNPDFVKYADSYGAKGYRVESAEHLEKLLAHCRDTPGVHLIDCPVDYSENDQILNKDIKELS
KAL

Sequences:

>Translated_563_residues
MAARLPQWERREMADGKKASDLFIECLEEEGCEYIFGVPGEENLDFLDSLSRSKKIRLVLTRHEQGAGFMAATYGRHTGK
TGVCIATLGPGATNFVTAAAYATLGGMPMLMITGQKPIKKSKQGRFQILDVVSMMGPITKFTHQMASSDNIPSRVREAYR
LAEEEKPGATHIELPEDIADEHTTSVPLKRSLVRRPNADAKSVAQAVHALQNAKAPVLVIGAGANRKMTGKMLLEFVEKT
GIPFLTTQLGKGVIDERHPKFLGCAALSSGDFVHRAVEDADIIINVGHDVIEKPPFFMREGGTPVIHVSTKTAEVDPVYF
PSIEVIGDIANAIWQMKEAITPNPAWNFDHMLAYRAAEVAHTAPLAADMRFPVFPPHLVQQVRDCMPEDGIICLDNGVYK
IWFARGYTAYKPNTVLLDNALATMGAGLPSAMMSAMLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDSAYG
MIRWKQANMGFEDFGLTYNNPDFVKYADSYGAKGYRVESAEHLEKLLAHCRDTPGVHLIDCPVDYSENDQILNKDIKELS
KAL
>Mature_562_residues
AARLPQWERREMADGKKASDLFIECLEEEGCEYIFGVPGEENLDFLDSLSRSKKIRLVLTRHEQGAGFMAATYGRHTGKT
GVCIATLGPGATNFVTAAAYATLGGMPMLMITGQKPIKKSKQGRFQILDVVSMMGPITKFTHQMASSDNIPSRVREAYRL
AEEEKPGATHIELPEDIADEHTTSVPLKRSLVRRPNADAKSVAQAVHALQNAKAPVLVIGAGANRKMTGKMLLEFVEKTG
IPFLTTQLGKGVIDERHPKFLGCAALSSGDFVHRAVEDADIIINVGHDVIEKPPFFMREGGTPVIHVSTKTAEVDPVYFP
SIEVIGDIANAIWQMKEAITPNPAWNFDHMLAYRAAEVAHTAPLAADMRFPVFPPHLVQQVRDCMPEDGIICLDNGVYKI
WFARGYTAYKPNTVLLDNALATMGAGLPSAMMSAMLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDSAYGM
IRWKQANMGFEDFGLTYNNPDFVKYADSYGAKGYRVESAEHLEKLLAHCRDTPGVHLIDCPVDYSENDQILNKDIKELSK
AL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=25.3054101221641, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI21361361, Length=561, Percent_Identity=26.0249554367201, Blast_Score=136, Evalue=6e-32,
Organism=Escherichia coli, GI1790104, Length=537, Percent_Identity=30.3538175046555, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI87081685, Length=561, Percent_Identity=27.0944741532977, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786717, Length=527, Percent_Identity=25.0474383301708, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788716, Length=581, Percent_Identity=25.473321858864, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1787096, Length=536, Percent_Identity=23.3208955223881, Blast_Score=125, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=475, Percent_Identity=25.4736842105263, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=475, Percent_Identity=25.4736842105263, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=537, Percent_Identity=22.7188081936685, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=30.4270462633452, Blast_Score=233, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6321524, Length=487, Percent_Identity=26.0780287474333, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=480, Percent_Identity=24.375, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320816, Length=494, Percent_Identity=23.6842105263158, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323073, Length=486, Percent_Identity=24.6913580246914, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922626, Length=486, Percent_Identity=25.7201646090535, Blast_Score=143, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61957; Mature: 61826

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARLPQWERREMADGKKASDLFIECLEEEGCEYIFGVPGEENLDFLDSLSRSKKIRLVL
CCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCCCCEEEEEE
TRHEQGAGFMAATYGRHTGKTGVCIATLGPGATNFVTAAAYATLGGMPMLMITGQKPIKK
EECCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHH
SKQGRFQILDVVSMMGPITKFTHQMASSDNIPSRVREAYRLAEEEKPGATHIELPEDIAD
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
EHTTSVPLKRSLVRRPNADAKSVAQAVHALQNAKAPVLVIGAGANRKMTGKMLLEFVEKT
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GIPFLTTQLGKGVIDERHPKFLGCAALSSGDFVHRAVEDADIIINVGHDVIEKPPFFMRE
CCCEEEHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCEEEEECCHHHHHCCCCCEEC
GGTPVIHVSTKTAEVDPVYFPSIEVIGDIANAIWQMKEAITPNPAWNFDHMLAYRAAEVA
CCCEEEEEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HTAPLAADMRFPVFPPHLVQQVRDCMPEDGIICLDNGVYKIWFARGYTAYKPNTVLLDNA
HHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEECCEEEECCCEEEECCH
LATMGAGLPSAMMSAMLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDSAYG
HHHHCCCCHHHHHHHHHCCCCEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEECCCCCE
MIRWKQANMGFEDFGLTYNNPDFVKYADSYGAKGYRVESAEHLEKLLAHCRDTPGVHLID
EEEEEECCCCHHHCCCCCCCCCCEEECHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEEE
CPVDYSENDQILNKDIKELSKAL
CCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AARLPQWERREMADGKKASDLFIECLEEEGCEYIFGVPGEENLDFLDSLSRSKKIRLVL
CCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCCCCEEEEEE
TRHEQGAGFMAATYGRHTGKTGVCIATLGPGATNFVTAAAYATLGGMPMLMITGQKPIKK
EECCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHH
SKQGRFQILDVVSMMGPITKFTHQMASSDNIPSRVREAYRLAEEEKPGATHIELPEDIAD
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
EHTTSVPLKRSLVRRPNADAKSVAQAVHALQNAKAPVLVIGAGANRKMTGKMLLEFVEKT
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GIPFLTTQLGKGVIDERHPKFLGCAALSSGDFVHRAVEDADIIINVGHDVIEKPPFFMRE
CCCEEEHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCEEEEECCHHHHHCCCCCEEC
GGTPVIHVSTKTAEVDPVYFPSIEVIGDIANAIWQMKEAITPNPAWNFDHMLAYRAAEVA
CCCEEEEEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HTAPLAADMRFPVFPPHLVQQVRDCMPEDGIICLDNGVYKIWFARGYTAYKPNTVLLDNA
HHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEECCEEEECCCEEEECCH
LATMGAGLPSAMMSAMLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDSAYG
HHHHCCCCHHHHHHHHHCCCCEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEECCCCCE
MIRWKQANMGFEDFGLTYNNPDFVKYADSYGAKGYRVESAEHLEKLLAHCRDTPGVHLID
EEEEEECCCCHHHCCCCCCCCCCEEECHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEEE
CPVDYSENDQILNKDIKELSKAL
CCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]