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The map label for this gene is purF [H]

Identifier: 87199780

GI number: 87199780

Start: 1858454

End: 1859944

Strand: Reverse

Name: purF [H]

Synonym: Saro_1763

Alternate gene names: 87199780

Gene position: 1859944-1858454 (Counterclockwise)

Preceding gene: 87199790

Following gene: 87199779

Centisome position: 52.22

GC content: 66.33

Gene sequence:

>1491_bases
ATGACTTCACAGAACGAACTTTTCGACAGCACGCCGTGGGACATCGGCAGTGACGACGACAAGCTGCGCGAAGAGTGCGG
CATCTTCGGCGTACTGGGCGTACGTGACGCCGCCGCGACAGTTGCACTGGGCCTCCACGCCCTGCAGCATCGGGGCCAGG
AAGCCGTCGGCATCACCAGTTTCGACGGGCAGGAGTTCTATTCGCGCAAGGGCATCGGCCACGTCGCGGCGAACTTCTCG
ACCGGCTCGGCCATCGCCGAACTGCCGGGCGCCATGGCCGCCGGCCACGTGCGCTACTCGACCACCGGCGGGGCCGGCCT
GCGCAACGTGCAGCCGCTGTTTGCCGACCTCGCGTCGGGCGGCTTCGCGATCGCCCACAACGGCAACATCTCGAACGCGA
TGTTCCTCAAGCGCGACCTCGTGCAGAAGGGCGCGATCTTCCAGTCGACGTCCGACACCGAAGTCATCATCCACCTCGTC
GCGACGAGCCGCTACCCCACCCTGCTCGACCGCTTCGTCGATGCCCTGCGGCTGGTCGAAGGCGCCTATTCGCTGATCTG
CATGACGCCCGAAGGCATGATCGCCTGCCGCGACCCGCTTGGCATCCGCCCGCTGGTCCTCGGCAAGATCGGCGACGCGG
TGGTCTTCGCGAGCGAAACCGTGGCGCTTGACGTGGTCGGCGCCGAATTCGTCCGCGAAGTGGAGCCGGGCGAACTGGTC
CAGGTCGATTTCGACGGCAAGCTCAGCAGCCACCGCCCGTTCGGCAGGCCAAGCGCGCGGCCCTGCATTTTCGAGCACGT
CTACTTCAGCCGTCCCGATTCGCTGATGGGCGGCAATTCCGTCTATCAGGTGCGCAAGGCCATCGGCGCGCAGCTCGCCA
TCGAAAGCCCGGCCGACGCGGACCTCGTCATCCCCGTGCCGGACAGCGGCGTTCCCGCCGCGCTTGGCTATGCCCAGCAG
TCCGGCATTCCGTTCGAGCTTGGCATCATCCGCTCGCACTATGTCGGCCGCACCTTCATCCAGCCTTCCGACGGTGCCCG
TAACGCAGACGTGAAGCGCAAACACAACGCGAACCGCGCACTGGTCGCGGGGAAGCGCATCGTGCTGATCGACGATTCGA
TCGTGCGCGGCACCACCAGCCTCAAGATCGTGCAGATGATGCGCGACGCGGGTGCGGCAGAAGTGCACATGCGCATCGCC
AGCCCGCCGACGGAGCATTCGTGCTTCTACGGCGTCGACACGCCCGAACGCTCGAAGCTGCTCGCCGCGCGGATGGACGT
GCAGGCCATGGCCGACTTCATCCATGCCGACAGCCTCGCCTTCGTCTCCATCGACGGTCTCTACCGCGCGGTCGGCGAGG
AGCAGCGCAACAAGGGCTGCCCGCAATATTGCGACGCCTGCTTCACCGGCGACTACCCCACGCGGCTGACCGACCTCGCA
GAGCGCGAGAATCCGGACCAGCTTTCGCTGCCGGTCGAAAAGGTCGCCTGA

Upstream 100 bases:

>100_bases
GCCTGGTTCCGCAGCTTGCACTTGCCTTCCGGGTCAAACGTCGCCATTTGGGCGCCAGCATTGGCCGGCGCGCAAGCGCC
CACGCGAGTCCACGGGACCC

Downstream 100 bases:

>100_bases
ATGAGCGCGCAGGGTTCGCAGGACGGCCGCTTTGCCGGCCAGTTGGCGCTCGTCACCGGCGCGAGCCGGGGCATCGGTGC
CGCCACGGCAAAGGCGCTTG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTSQNELFDSTPWDIGSDDDKLREECGIFGVLGVRDAAATVALGLHALQHRGQEAVGITSFDGQEFYSRKGIGHVAANFS
TGSAIAELPGAMAAGHVRYSTTGGAGLRNVQPLFADLASGGFAIAHNGNISNAMFLKRDLVQKGAIFQSTSDTEVIIHLV
ATSRYPTLLDRFVDALRLVEGAYSLICMTPEGMIACRDPLGIRPLVLGKIGDAVVFASETVALDVVGAEFVREVEPGELV
QVDFDGKLSSHRPFGRPSARPCIFEHVYFSRPDSLMGGNSVYQVRKAIGAQLAIESPADADLVIPVPDSGVPAALGYAQQ
SGIPFELGIIRSHYVGRTFIQPSDGARNADVKRKHNANRALVAGKRIVLIDDSIVRGTTSLKIVQMMRDAGAAEVHMRIA
SPPTEHSCFYGVDTPERSKLLAARMDVQAMADFIHADSLAFVSIDGLYRAVGEEQRNKGCPQYCDACFTGDYPTRLTDLA
ERENPDQLSLPVEKVA

Sequences:

>Translated_496_residues
MTSQNELFDSTPWDIGSDDDKLREECGIFGVLGVRDAAATVALGLHALQHRGQEAVGITSFDGQEFYSRKGIGHVAANFS
TGSAIAELPGAMAAGHVRYSTTGGAGLRNVQPLFADLASGGFAIAHNGNISNAMFLKRDLVQKGAIFQSTSDTEVIIHLV
ATSRYPTLLDRFVDALRLVEGAYSLICMTPEGMIACRDPLGIRPLVLGKIGDAVVFASETVALDVVGAEFVREVEPGELV
QVDFDGKLSSHRPFGRPSARPCIFEHVYFSRPDSLMGGNSVYQVRKAIGAQLAIESPADADLVIPVPDSGVPAALGYAQQ
SGIPFELGIIRSHYVGRTFIQPSDGARNADVKRKHNANRALVAGKRIVLIDDSIVRGTTSLKIVQMMRDAGAAEVHMRIA
SPPTEHSCFYGVDTPERSKLLAARMDVQAMADFIHADSLAFVSIDGLYRAVGEEQRNKGCPQYCDACFTGDYPTRLTDLA
ERENPDQLSLPVEKVA
>Mature_495_residues
TSQNELFDSTPWDIGSDDDKLREECGIFGVLGVRDAAATVALGLHALQHRGQEAVGITSFDGQEFYSRKGIGHVAANFST
GSAIAELPGAMAAGHVRYSTTGGAGLRNVQPLFADLASGGFAIAHNGNISNAMFLKRDLVQKGAIFQSTSDTEVIIHLVA
TSRYPTLLDRFVDALRLVEGAYSLICMTPEGMIACRDPLGIRPLVLGKIGDAVVFASETVALDVVGAEFVREVEPGELVQ
VDFDGKLSSHRPFGRPSARPCIFEHVYFSRPDSLMGGNSVYQVRKAIGAQLAIESPADADLVIPVPDSGVPAALGYAQQS
GIPFELGIIRSHYVGRTFIQPSDGARNADVKRKHNANRALVAGKRIVLIDDSIVRGTTSLKIVQMMRDAGAAEVHMRIAS
PPTEHSCFYGVDTPERSKLLAARMDVQAMADFIHADSLAFVSIDGLYRAVGEEQRNKGCPQYCDACFTGDYPTRLTDLAE
RENPDQLSLPVEKVA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=36.9351669941061, Blast_Score=328, Evalue=1e-89,
Organism=Escherichia coli, GI1788651, Length=475, Percent_Identity=38.5263157894737, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1790167, Length=223, Percent_Identity=29.1479820627803, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=37.7870563674322, Blast_Score=292, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6323958, Length=481, Percent_Identity=32.6403326403326, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24659598, Length=515, Percent_Identity=40.1941747572816, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI28573187, Length=511, Percent_Identity=40.3131115459883, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24659604, Length=484, Percent_Identity=41.1157024793388, Blast_Score=338, Evalue=5e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53321; Mature: 53190

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQNELFDSTPWDIGSDDDKLREECGIFGVLGVRDAAATVALGLHALQHRGQEAVGITS
CCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEC
FDGQEFYSRKGIGHVAANFSTGSAIAELPGAMAAGHVRYSTTGGAGLRNVQPLFADLASG
CCCHHHHHHCCCCEEEECCCCCCHHHHCCCCHHCCCEEEECCCCCCCCCCHHHHHHHCCC
GFAIAHNGNISNAMFLKRDLVQKGAIFQSTSDTEVIIHLVATSRYPTLLDRFVDALRLVE
CEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
GAYSLICMTPEGMIACRDPLGIRPLVLGKIGDAVVFASETVALDVVGAEFVREVEPGELV
CCEEEEEECCCCCEEECCCCCCCCEEEECCCCEEEEECCEEEEEEHHHHHHHHCCCCCEE
QVDFDGKLSSHRPFGRPSARPCIFEHVYFSRPDSLMGGNSVYQVRKAIGAQLAIESPADA
EEEECCCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHCCEEEECCCCCC
DLVIPVPDSGVPAALGYAQQSGIPFELGIIRSHYVGRTFIQPSDGARNADVKRKHNANRA
EEEEECCCCCCCHHHHHHHHCCCCEEEEEHHHHCCCCEEECCCCCCCCCCCHHHCCCCEE
LVAGKRIVLIDDSIVRGTTSLKIVQMMRDAGAAEVHMRIASPPTEHSCFYGVDTPERSKL
EEECCEEEEECCCHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHH
LAARMDVQAMADFIHADSLAFVSIDGLYRAVGEEQRNKGCPQYCDACFTGDYPTRLTDLA
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCHHHHCCCCHHHHHHHCCCCCCHHHHHHH
ERENPDQLSLPVEKVA
HCCCCCCCCCCHHHCC
>Mature Secondary Structure 
TSQNELFDSTPWDIGSDDDKLREECGIFGVLGVRDAAATVALGLHALQHRGQEAVGITS
CCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEC
FDGQEFYSRKGIGHVAANFSTGSAIAELPGAMAAGHVRYSTTGGAGLRNVQPLFADLASG
CCCHHHHHHCCCCEEEECCCCCCHHHHCCCCHHCCCEEEECCCCCCCCCCHHHHHHHCCC
GFAIAHNGNISNAMFLKRDLVQKGAIFQSTSDTEVIIHLVATSRYPTLLDRFVDALRLVE
CEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
GAYSLICMTPEGMIACRDPLGIRPLVLGKIGDAVVFASETVALDVVGAEFVREVEPGELV
CCEEEEEECCCCCEEECCCCCCCCEEEECCCCEEEEECCEEEEEEHHHHHHHHCCCCCEE
QVDFDGKLSSHRPFGRPSARPCIFEHVYFSRPDSLMGGNSVYQVRKAIGAQLAIESPADA
EEEECCCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHCCEEEECCCCCC
DLVIPVPDSGVPAALGYAQQSGIPFELGIIRSHYVGRTFIQPSDGARNADVKRKHNANRA
EEEEECCCCCCCHHHHHHHHCCCCEEEEEHHHHCCCCEEECCCCCCCCCCCHHHCCCCEE
LVAGKRIVLIDDSIVRGTTSLKIVQMMRDAGAAEVHMRIASPPTEHSCFYGVDTPERSKL
EEECCEEEEECCCHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHH
LAARMDVQAMADFIHADSLAFVSIDGLYRAVGEEQRNKGCPQYCDACFTGDYPTRLTDLA
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCHHHHCCCCHHHHHHHCCCCCCHHHHHHH
ERENPDQLSLPVEKVA
HCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA