Click here to switch to the map view.

The map label for this gene is glgX [H]

Identifier: 87199671

GI number: 87199671

Start: 1729430

End: 1731196

Strand: Reverse

Name: glgX [H]

Synonym: Saro_1654

Alternate gene names: 87199671

Gene position: 1731196-1729430 (Counterclockwise)

Preceding gene: 87199672

Following gene: 87199665

Centisome position: 48.61

GC content: 63.33

Gene sequence:

>1767_bases
GTGACGCCGGGCGTCTTTCTGGCGGACGGCGCCACCCGCTTCTCCGTCCGCTCCCCTCGCGCCGAGGCACTGACGCTCTG
CCTGTTCGAGGACGAAGCCGAGCAGCGTATCGCGATGACCCGCGTCGGCGATAACTGGATCGTTGAAATTCCAAGGGATC
TGCGTGGCATTCGCTACGGCTACCGCGCGTCTGGCGAATGGAACCCGCCGGATGGTCTGTGGTTCGATCCTGCCAAGTTG
CTTGTCGATCCCTACGCAATCGAGTTGGACGGCAGCTTTGTGCAACACACGAGCTTGTCCACCTACGGCGCTGACACGTC
GAAAATTGTTCCCAAAGCAGTGGTTACCGCACCGCCGGTGATTTCTCACCTCTCGCCGCTGTTCCGCCACGGCGGGTTGA
TCTACGAAGTCAACGTGCGCGGGCTTACCATGCTCCATCCGGATGTCCCGCAGCCGCTCCGGGGCACCGTCGCCGCGCTC
GCTCACCCGGCAATCATCGGCCATCTCAAGCAGTTGCACGTCTCGGCGGTGGAACTCATGCCAATCGTCGCCTGGATCGA
CGAGCGGCACCTGCCCCCGCTCGGCCTCACCAACGCCTGGGGATACAACCCCGTCGCCCCCATGGCTCTCGATCCCCGCG
TCTGTCCGGGCGGAGTCAGGGAACTGCGCGAAACCGTGGCCACGTTGCGAGCCGAAGGGATCGGCGTAATCCTCGATATC
GTATTGAATCACAGCGGGGAATCCGACGTTCTCGGCCCCGTCCTTTGCCTCCGCGGTCTCGATGACGCGGCCTATACCAA
GGCGCCCGACGGTAGGCTGATCAACGACACCGGCTGCGGCAATACGCTCGATTTCGGAAATCCTGCCGTTCGCGCGCTGG
CGCTCGATACCCTGCGCCACTTCACAAGGCATTGCGGCGTCGACGGTTTCCGCTTCGACCTCGCTCCCATCCTCGCCCGC
AGCCCCGGCTTCGCCGCCGACGCGCCGATCTTCCGCGAGATCGCCGCCGACCCGGTGCTGGCCGACCGGGTGATGATCGC
GGAGCCTTGGGACATTGGGCCCGGGGGATACCAGCTCGGAGGTTTCCCTTCTAACTGGCTGGAATGGAACGACAGATTCC
GAGACGACGTACGACGATTCTGGAAGGGTGAGACCGGCGTCGGCGCGCTTGCAACGCGGATTGCCGGGTCTTCCGATATC
TTCGGCAAGGCCTGCCGAACGGTCAATTTCCTGGCGGCCCACGACGGCTTTACTCTCGCCGATACGCTTGCCTACGAGCA
GCGCCACAACTGGGCGAACGGCGAGGAGAACCGCGATGGACACGGCGAAAATTTTAGCTGGAACAACGGCATGGAGGGAG
AAACGGACAATCTGGACGTCCTTTCCCGCCGCGCCGCCCTTGTACGCGCCATGCTCGGGACACTGTTCATTTCGACCGGT
ACGATCCTGCTGACCGCCGGCGACGAATTTGGCCGGAGCCAGCGCGGCAACAACAATGCCTATGCGCAGGACAACGAAAT
CACCTGGATCGACTGGGACCGGCGGGACCGTGCCCTGGAAGCGTGGACCTTCGGGCTATCCGCCTGGCGCCATTTGAGAA
TTTCATCCTTCACCAGGTTTCCGGAGGATGGAACCTGGCGTGACCTGAACGGCACCGAACTGCACACGAGCGCGTGGGAA
AGCGACCTGACCCAGGGATTTTTCTGGCAGAATGGCAGCTACGGCTTCGTGGTCGACCGGACCGCCCCGATGGCAGGTCC
GGTCTGA

Upstream 100 bases:

>100_bases
ACCGTGAAACGCCTGAAATGCTTGCCGATCTCATCCGGGCGGCAGACGCCATCGCCGGCATCGCGCGTCACACCGGACGC
ACCGCGCCCGACGTGGTGAC

Downstream 100 bases:

>100_bases
CGGTCACGCTCCCAACCGGCGCAGCGCCCTGTCCGGTTGGAAGTTGCCAACTACCCCGCGGGAAGCGGAACTGTCCAGCG
CAAGCGGCCGGGGCAAGAGC

Product: isoamylase

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MTPGVFLADGATRFSVRSPRAEALTLCLFEDEAEQRIAMTRVGDNWIVEIPRDLRGIRYGYRASGEWNPPDGLWFDPAKL
LVDPYAIELDGSFVQHTSLSTYGADTSKIVPKAVVTAPPVISHLSPLFRHGGLIYEVNVRGLTMLHPDVPQPLRGTVAAL
AHPAIIGHLKQLHVSAVELMPIVAWIDERHLPPLGLTNAWGYNPVAPMALDPRVCPGGVRELRETVATLRAEGIGVILDI
VLNHSGESDVLGPVLCLRGLDDAAYTKAPDGRLINDTGCGNTLDFGNPAVRALALDTLRHFTRHCGVDGFRFDLAPILAR
SPGFAADAPIFREIAADPVLADRVMIAEPWDIGPGGYQLGGFPSNWLEWNDRFRDDVRRFWKGETGVGALATRIAGSSDI
FGKACRTVNFLAAHDGFTLADTLAYEQRHNWANGEENRDGHGENFSWNNGMEGETDNLDVLSRRAALVRAMLGTLFISTG
TILLTAGDEFGRSQRGNNNAYAQDNEITWIDWDRRDRALEAWTFGLSAWRHLRISSFTRFPEDGTWRDLNGTELHTSAWE
SDLTQGFFWQNGSYGFVVDRTAPMAGPV

Sequences:

>Translated_588_residues
MTPGVFLADGATRFSVRSPRAEALTLCLFEDEAEQRIAMTRVGDNWIVEIPRDLRGIRYGYRASGEWNPPDGLWFDPAKL
LVDPYAIELDGSFVQHTSLSTYGADTSKIVPKAVVTAPPVISHLSPLFRHGGLIYEVNVRGLTMLHPDVPQPLRGTVAAL
AHPAIIGHLKQLHVSAVELMPIVAWIDERHLPPLGLTNAWGYNPVAPMALDPRVCPGGVRELRETVATLRAEGIGVILDI
VLNHSGESDVLGPVLCLRGLDDAAYTKAPDGRLINDTGCGNTLDFGNPAVRALALDTLRHFTRHCGVDGFRFDLAPILAR
SPGFAADAPIFREIAADPVLADRVMIAEPWDIGPGGYQLGGFPSNWLEWNDRFRDDVRRFWKGETGVGALATRIAGSSDI
FGKACRTVNFLAAHDGFTLADTLAYEQRHNWANGEENRDGHGENFSWNNGMEGETDNLDVLSRRAALVRAMLGTLFISTG
TILLTAGDEFGRSQRGNNNAYAQDNEITWIDWDRRDRALEAWTFGLSAWRHLRISSFTRFPEDGTWRDLNGTELHTSAWE
SDLTQGFFWQNGSYGFVVDRTAPMAGPV
>Mature_587_residues
TPGVFLADGATRFSVRSPRAEALTLCLFEDEAEQRIAMTRVGDNWIVEIPRDLRGIRYGYRASGEWNPPDGLWFDPAKLL
VDPYAIELDGSFVQHTSLSTYGADTSKIVPKAVVTAPPVISHLSPLFRHGGLIYEVNVRGLTMLHPDVPQPLRGTVAALA
HPAIIGHLKQLHVSAVELMPIVAWIDERHLPPLGLTNAWGYNPVAPMALDPRVCPGGVRELRETVATLRAEGIGVILDIV
LNHSGESDVLGPVLCLRGLDDAAYTKAPDGRLINDTGCGNTLDFGNPAVRALALDTLRHFTRHCGVDGFRFDLAPILARS
PGFAADAPIFREIAADPVLADRVMIAEPWDIGPGGYQLGGFPSNWLEWNDRFRDDVRRFWKGETGVGALATRIAGSSDIF
GKACRTVNFLAAHDGFTLADTLAYEQRHNWANGEENRDGHGENFSWNNGMEGETDNLDVLSRRAALVRAMLGTLFISTGT
ILLTAGDEFGRSQRGNNNAYAQDNEITWIDWDRRDRALEAWTFGLSAWRHLRISSFTRFPEDGTWRDLNGTELHTSAWES
DLTQGFFWQNGSYGFVVDRTAPMAGPV

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=585, Percent_Identity=44.1025641025641, Blast_Score=445, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 64761; Mature: 64629

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGVFLADGATRFSVRSPRAEALTLCLFEDEAEQRIAMTRVGDNWIVEIPRDLRGIRYG
CCCCEEEECCCEEEEECCCCCCEEEEEEECCCHHHEEEEEECCCCEEEECCHHHHHCCCC
YRASGEWNPPDGLWFDPAKLLVDPYAIELDGSFVQHTSLSTYGADTSKIVPKAVVTAPPV
EECCCCCCCCCCCEECHHHHHCCCEEEEECCCEEECCCCCCCCCCCHHHCCHHHHCCCHH
ISHLSPLFRHGGLIYEVNVRGLTMLHPDVPQPLRGTVAALAHPAIIGHLKQLHVSAVELM
HHHHHHHHHCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIVAWIDERHLPPLGLTNAWGYNPVAPMALDPRVCPGGVRELRETVATLRAEGIGVILDI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
VLNHSGESDVLGPVLCLRGLDDAAYTKAPDGRLINDTGCGNTLDFGNPAVRALALDTLRH
EECCCCCCCHHHHHHHHHCCCHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHH
FTRHCGVDGFRFDLAPILARSPGFAADAPIFREIAADPVLADRVMIAEPWDIGPGGYQLG
HHHHCCCCCEEEHHHHHHHCCCCCCCCCHHHHHHHCCCHHHCEEEEECCCCCCCCCEECC
GFPSNWLEWNDRFRDDVRRFWKGETGVGALATRIAGSSDIFGKACRTVNFLAAHDGFTLA
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCHH
DTLAYEQRHNWANGEENRDGHGENFSWNNGMEGETDNLDVLSRRAALVRAMLGTLFISTG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TILLTAGDEFGRSQRGNNNAYAQDNEITWIDWDRRDRALEAWTFGLSAWRHLRISSFTRF
EEEEECCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PEDGTWRDLNGTELHTSAWESDLTQGFFWQNGSYGFVVDRTAPMAGPV
CCCCCEECCCCCEEEHHHHHHHHHCCEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
TPGVFLADGATRFSVRSPRAEALTLCLFEDEAEQRIAMTRVGDNWIVEIPRDLRGIRYG
CCCEEEECCCEEEEECCCCCCEEEEEEECCCHHHEEEEEECCCCEEEECCHHHHHCCCC
YRASGEWNPPDGLWFDPAKLLVDPYAIELDGSFVQHTSLSTYGADTSKIVPKAVVTAPPV
EECCCCCCCCCCCEECHHHHHCCCEEEEECCCEEECCCCCCCCCCCHHHCCHHHHCCCHH
ISHLSPLFRHGGLIYEVNVRGLTMLHPDVPQPLRGTVAALAHPAIIGHLKQLHVSAVELM
HHHHHHHHHCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIVAWIDERHLPPLGLTNAWGYNPVAPMALDPRVCPGGVRELRETVATLRAEGIGVILDI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
VLNHSGESDVLGPVLCLRGLDDAAYTKAPDGRLINDTGCGNTLDFGNPAVRALALDTLRH
EECCCCCCCHHHHHHHHHCCCHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHH
FTRHCGVDGFRFDLAPILARSPGFAADAPIFREIAADPVLADRVMIAEPWDIGPGGYQLG
HHHHCCCCCEEEHHHHHHHCCCCCCCCCHHHHHHHCCCHHHCEEEEECCCCCCCCCEECC
GFPSNWLEWNDRFRDDVRRFWKGETGVGALATRIAGSSDIFGKACRTVNFLAAHDGFTLA
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCHH
DTLAYEQRHNWANGEENRDGHGENFSWNNGMEGETDNLDVLSRRAALVRAMLGTLFISTG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TILLTAGDEFGRSQRGNNNAYAQDNEITWIDWDRRDRALEAWTFGLSAWRHLRISSFTRF
EEEEECCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PEDGTWRDLNGTELHTSAWESDLTQGFFWQNGSYGFVVDRTAPMAGPV
CCCCCEECCCCCEEEHHHHHHHHHCCEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA