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The map label for this gene is aes [H]

Identifier: 87199602

GI number: 87199602

Start: 1642692

End: 1643663

Strand: Reverse

Name: aes [H]

Synonym: Saro_1585

Alternate gene names: 87199602

Gene position: 1643663-1642692 (Counterclockwise)

Preceding gene: 87199603

Following gene: 87199601

Centisome position: 46.15

GC content: 67.49

Gene sequence:

>972_bases
ATGACCGGAAAGTTCCTCAGTGCGGCCCTGCTCGCGGTCCTTGCCGCGCAGCCTGCGACGGCGAAGGAAAGGGTGTTCCC
GACCGCCGACAAGCCCGTCCTGGAAGACCGCTTTCCGCAAGTCCCGGTCGCCTTCCCCGGCGGCGTGAAGGCATGGCGCG
ACGTGACCTACCAGACCATCGACGGATACCGCCCGCAGGTCGTCGACATCTACGTCCCTGCGGGCAAGGGCCCGCATCCG
CTGGTGCTTTATATTCACGGTGGCGGCTGGCGTGGCGGACATACCCGGCACTCGGGCGCCTTTGCCGATTTCCCCAAGGT
TCTCGCGGCTTTGGCTGCCGAGGGCTTCACGGTGGCGAGCCTGGAATACCGTCTCTCCAGTGAAGCGCGGTTCCCCGCGC
AGTTGCAGGATTCGAACGCGGCGCTGCGCTTCCTGCGCGCCAACGCCGCGCGCTATGCCATTGATCCGGCGCGCGTTGGC
GTATGGGGCGGGTCGGCGGGCGGCCACCTCACCGCCCTCACCGCGCTGACCTGCCGCGACACGGCCCTCGATCCCGCCGC
TGCGCAGGATGGCTGCGTCCAGGCGGCGGTGACGTGGTACGGCGTCTATGACTTCGCCGGCATGAACGCGACGCCCGATG
GCAACTCGGCGGGCGGCAAGCTGCTCGGCTGCGAAGGGCCGTGCAGCGCCGACAAGAACCGGCTGGTCAGTCCCGTCGCC
TACATCGACGCCAAGGACCCGCCGTTCCTGCTGATCCACGGCGAGGAGGACAAGGTCGTCCCGGCCGAACAGTCGCGGCT
GGGCGAAGCTGCGCTGAAGGCCGCCGGGGTGCCGGTCAAGTCGATCTACATTCCCGGTGTCGACCACAGCTTCATCGGCA
AGACTCCCGCGGAAACCCGCGCTGCATCGCTCAAGGCGGTGAATGCAACATTCGATTTCTTCCACGAGAAGCTGGGAGTG
CCGCGCAAGTGA

Upstream 100 bases:

>100_bases
GCCCCGATCCGTCTCGCTGCGCAGGCTCGGCACAAGCCTGACCTGAACGGCCTCTAACCATACACGAGACGCAAAAAAGC
ATACCCGAAGGGAGAGAAGA

Downstream 100 bases:

>100_bases
TCCGGCGGCTGATCGGCACGTTGCTGCTGGGCCTCGCGGCGCCGGCAACCGCCGCTCCGGTCGCGGTCGACGGCGGCATG
GTCGAGGGGATCGAACTGCC

Product: dienelactone hydrolase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTGKFLSAALLAVLAAQPATAKERVFPTADKPVLEDRFPQVPVAFPGGVKAWRDVTYQTIDGYRPQVVDIYVPAGKGPHP
LVLYIHGGGWRGGHTRHSGAFADFPKVLAALAAEGFTVASLEYRLSSEARFPAQLQDSNAALRFLRANAARYAIDPARVG
VWGGSAGGHLTALTALTCRDTALDPAAAQDGCVQAAVTWYGVYDFAGMNATPDGNSAGGKLLGCEGPCSADKNRLVSPVA
YIDAKDPPFLLIHGEEDKVVPAEQSRLGEAALKAAGVPVKSIYIPGVDHSFIGKTPAETRAASLKAVNATFDFFHEKLGV
PRK

Sequences:

>Translated_323_residues
MTGKFLSAALLAVLAAQPATAKERVFPTADKPVLEDRFPQVPVAFPGGVKAWRDVTYQTIDGYRPQVVDIYVPAGKGPHP
LVLYIHGGGWRGGHTRHSGAFADFPKVLAALAAEGFTVASLEYRLSSEARFPAQLQDSNAALRFLRANAARYAIDPARVG
VWGGSAGGHLTALTALTCRDTALDPAAAQDGCVQAAVTWYGVYDFAGMNATPDGNSAGGKLLGCEGPCSADKNRLVSPVA
YIDAKDPPFLLIHGEEDKVVPAEQSRLGEAALKAAGVPVKSIYIPGVDHSFIGKTPAETRAASLKAVNATFDFFHEKLGV
PRK
>Mature_322_residues
TGKFLSAALLAVLAAQPATAKERVFPTADKPVLEDRFPQVPVAFPGGVKAWRDVTYQTIDGYRPQVVDIYVPAGKGPHPL
VLYIHGGGWRGGHTRHSGAFADFPKVLAALAAEGFTVASLEYRLSSEARFPAQLQDSNAALRFLRANAARYAIDPARVGV
WGGSAGGHLTALTALTCRDTALDPAAAQDGCVQAAVTWYGVYDFAGMNATPDGNSAGGKLLGCEGPCSADKNRLVSPVAY
IDAKDPPFLLIHGEEDKVVPAEQSRLGEAALKAAGVPVKSIYIPGVDHSFIGKTPAETRAASLKAVNATFDFFHEKLGVP
RK

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=243, Percent_Identity=27.1604938271605, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34094; Mature: 33963

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00498 TYROSINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKFLSAALLAVLAAQPATAKERVFPTADKPVLEDRFPQVPVAFPGGVKAWRDVTYQTI
CCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCCCCEECCCCCHHHHHCHHHHC
DGYRPQVVDIYVPAGKGPHPLVLYIHGGGWRGGHTRHSGAFADFPKVLAALAAEGFTVAS
CCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
LEYRLSSEARFPAQLQDSNAALRFLRANAARYAIDPARVGVWGGSAGGHLTALTALTCRD
EEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEECHHHEEECCCCCCCCEEEEEEEEECC
TALDPAAAQDGCVQAAVTWYGVYDFAGMNATPDGNSAGGKLLGCEGPCSADKNRLVSPVA
CCCCCCCCCCCHHEEHHHEEEHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEE
YIDAKDPPFLLIHGEEDKVVPAEQSRLGEAALKAAGVPVKSIYIPGVDHSFIGKTPAETR
EEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCCCCHHH
AASLKAVNATFDFFHEKLGVPRK
HHHHEEEHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TGKFLSAALLAVLAAQPATAKERVFPTADKPVLEDRFPQVPVAFPGGVKAWRDVTYQTI
CCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCCCCEECCCCCHHHHHCHHHHC
DGYRPQVVDIYVPAGKGPHPLVLYIHGGGWRGGHTRHSGAFADFPKVLAALAAEGFTVAS
CCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
LEYRLSSEARFPAQLQDSNAALRFLRANAARYAIDPARVGVWGGSAGGHLTALTALTCRD
EEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEECHHHEEECCCCCCCCEEEEEEEEECC
TALDPAAAQDGCVQAAVTWYGVYDFAGMNATPDGNSAGGKLLGCEGPCSADKNRLVSPVA
CCCCCCCCCCCHHEEHHHEEEHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEE
YIDAKDPPFLLIHGEEDKVVPAEQSRLGEAALKAAGVPVKSIYIPGVDHSFIGKTPAETR
EEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCCCCHHH
AASLKAVNATFDFFHEKLGVPRK
HHHHEEEHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA