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The map label for this gene is mraW

Identifier: 87199147

GI number: 87199147

Start: 1168835

End: 1169824

Strand: Direct

Name: mraW

Synonym: Saro_1125

Alternate gene names: 87199147

Gene position: 1168835-1169824 (Clockwise)

Preceding gene: 87199146

Following gene: 87199148

Centisome position: 32.82

GC content: 66.67

Gene sequence:

>990_bases
GTGAGCGCCGAAACCGGCTCCCGTGCCCCGCACATACCCGTTCTCCTCGAAGAGGTCGTGGCTGCACTCGACCCCAAGCC
GGGTGACCTGATCGTCGATGCGACCTTCGGGGCCGGCGGCTATACGCGTCGGCTCCTTGATGCAGGCGCCACCGTCCACG
CCTTCGACCGGGACCCCGATGCCATCGCGGCTGGGAAGCTCTGGGGAGAAACCTGTGGAAAAGAGCCGCGCCTGGTTCTG
CATCCTCGTCGTTTTTCCGAGATCGCGGAAGGACTTGCAGAGGCCGGAATTGCTGGAGTCCAGGGGGTGGTCTTCGACAT
CGGGGTCAGCTCGATGCAGCTCGATCAGGCCGCGCGCGGATTTGCGTTTTCGTCCGACGGTCCCTTAGACATGCGAATGT
CGCAGGAAGGGCCGAGTGCGGCGGACTTCCTGAACGAAGCGGACGAGGGGGAGATCGCGGACGTCCTCTATCGCTACGGC
GAAGAACGCCAATCGCGTCGGATTGCCCGCGCCATCGTTGCTGCCCGTCCGCTGACCACGACCGCCCAGTTCGCTGCCGT
CGTCCGCAAGGCTCTTGGCTACCGGCCCGACATCAAGGGTCCCAAGGCGCCCAAGGATCCTGCCACCCGCAGTTTCCAGG
CCGTGCGCATCCACGTAAACGGTGAGCTCGATGAGCTGGTGCAGGGGCTCGCTGCCGCAGAGAAGGTGCTTGTGCCCGGC
GGCCGGATCGCCGTGGTCAGCTTTCACAGCCTCGAGGACCGGATCGTGAAGCAGTTCCTGCGCGAGGGCGCGGGTGCCGT
TCCCGCCGGTTCGCGTCACCTGCCGCAGCTTGAAAGCAAGGTTCAGGCCGTGTTCGAGAAGCCTTCGGGTGCGATCAGGC
CGACGCCGGAGGAAGAGGCCCGCAATCCTCGCGCCCGCTCCGCGACCCTGCGTTGTGCCGTGCGCACTGCCGCGCCGGCG
CGCGCTCATCAGGGGAGGGCCGCGGCATGA

Upstream 100 bases:

>100_bases
TCTGGAACCCGGGCATCCTGTTCGAACAGGGCGCTGGTTGGGAAAGCGCCCAGGCCGGTTGCCGCGCGAAGATCGCCGAA
GCCGCGAAGGGGAAGCGCAA

Downstream 100 bases:

>100_bases
TCGCTTCGGCCAATCGCCTTCGTTCGGTAGGCTGGCTCGTCCTGCTGGGCCTCTGTCTGGCGCTGGTTCTGGTTCTCGCG
TTCAAGGTCAACGCGCTGCG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSAETGSRAPHIPVLLEEVVAALDPKPGDLIVDATFGAGGYTRRLLDAGATVHAFDRDPDAIAAGKLWGETCGKEPRLVL
HPRRFSEIAEGLAEAGIAGVQGVVFDIGVSSMQLDQAARGFAFSSDGPLDMRMSQEGPSAADFLNEADEGEIADVLYRYG
EERQSRRIARAIVAARPLTTTAQFAAVVRKALGYRPDIKGPKAPKDPATRSFQAVRIHVNGELDELVQGLAAAEKVLVPG
GRIAVVSFHSLEDRIVKQFLREGAGAVPAGSRHLPQLESKVQAVFEKPSGAIRPTPEEEARNPRARSATLRCAVRTAAPA
RAHQGRAAA

Sequences:

>Translated_329_residues
MSAETGSRAPHIPVLLEEVVAALDPKPGDLIVDATFGAGGYTRRLLDAGATVHAFDRDPDAIAAGKLWGETCGKEPRLVL
HPRRFSEIAEGLAEAGIAGVQGVVFDIGVSSMQLDQAARGFAFSSDGPLDMRMSQEGPSAADFLNEADEGEIADVLYRYG
EERQSRRIARAIVAARPLTTTAQFAAVVRKALGYRPDIKGPKAPKDPATRSFQAVRIHVNGELDELVQGLAAAEKVLVPG
GRIAVVSFHSLEDRIVKQFLREGAGAVPAGSRHLPQLESKVQAVFEKPSGAIRPTPEEEARNPRARSATLRCAVRTAAPA
RAHQGRAAA
>Mature_328_residues
SAETGSRAPHIPVLLEEVVAALDPKPGDLIVDATFGAGGYTRRLLDAGATVHAFDRDPDAIAAGKLWGETCGKEPRLVLH
PRRFSEIAEGLAEAGIAGVQGVVFDIGVSSMQLDQAARGFAFSSDGPLDMRMSQEGPSAADFLNEADEGEIADVLYRYGE
ERQSRRIARAIVAARPLTTTAQFAAVVRKALGYRPDIKGPKAPKDPATRSFQAVRIHVNGELDELVQGLAAAEKVLVPGG
RIAVVSFHSLEDRIVKQFLREGAGAVPAGSRHLPQLESKVQAVFEKPSGAIRPTPEEEARNPRARSATLRCAVRTAAPAR
AHQGRAAA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=39.2330383480826, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=42.3280423280423, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1786270, Length=321, Percent_Identity=45.7943925233645, Blast_Score=233, Evalue=1e-62,
Organism=Drosophila melanogaster, GI62472493, Length=335, Percent_Identity=40.5970149253731, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI28571637, Length=335, Percent_Identity=40.5970149253731, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_NOVAD (Q2G9A3)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496404.1
- ProteinModelPortal:   Q2G9A3
- SMR:   Q2G9A3
- STRING:   Q2G9A3
- GeneID:   3916421
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1125
- NMPDR:   fig|48935.1.peg.307
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- PhylomeDB:   Q2G9A3
- ProtClustDB:   PRK00050
- BioCyc:   NARO279238:SARO_1125-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34987; Mature: 34856

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 85-85 BINDING 106-106 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAETGSRAPHIPVLLEEVVAALDPKPGDLIVDATFGAGGYTRRLLDAGATVHAFDRDPD
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCEEEECCCCCC
AIAAGKLWGETCGKEPRLVLHPRRFSEIAEGLAEAGIAGVQGVVFDIGVSSMQLDQAARG
HHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHEECCCCHHHHHHHHCC
FAFSSDGPLDMRMSQEGPSAADFLNEADEGEIADVLYRYGEERQSRRIARAIVAARPLTT
CCCCCCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TAQFAAVVRKALGYRPDIKGPKAPKDPATRSFQAVRIHVNGELDELVQGLAAAEKVLVPG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHEECCC
GRIAVVSFHSLEDRIVKQFLREGAGAVPAGSRHLPQLESKVQAVFEKPSGAIRPTPEEEA
CCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
RNPRARSATLRCAVRTAAPARAHQGRAAA
CCCCHHHHHHEEEHHHCCCHHHCCCCCCC
>Mature Secondary Structure 
SAETGSRAPHIPVLLEEVVAALDPKPGDLIVDATFGAGGYTRRLLDAGATVHAFDRDPD
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCEEEECCCCCC
AIAAGKLWGETCGKEPRLVLHPRRFSEIAEGLAEAGIAGVQGVVFDIGVSSMQLDQAARG
HHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHEECCCCHHHHHHHHCC
FAFSSDGPLDMRMSQEGPSAADFLNEADEGEIADVLYRYGEERQSRRIARAIVAARPLTT
CCCCCCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TAQFAAVVRKALGYRPDIKGPKAPKDPATRSFQAVRIHVNGELDELVQGLAAAEKVLVPG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHEECCC
GRIAVVSFHSLEDRIVKQFLREGAGAVPAGSRHLPQLESKVQAVFEKPSGAIRPTPEEEA
CCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
RNPRARSATLRCAVRTAAPARAHQGRAAA
CCCCHHHHHHEEEHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA