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The map label for this gene is mscL

Identifier: 87198902

GI number: 87198902

Start: 934521

End: 934988

Strand: Direct

Name: mscL

Synonym: Saro_0880

Alternate gene names: 87198902

Gene position: 934521-934988 (Clockwise)

Preceding gene: 87198896

Following gene: 87198907

Centisome position: 26.24

GC content: 62.61

Gene sequence:

>468_bases
ATGGCGCACGAAGTGAAGGGGGAGATCCTGGGTATGCTGGGCGAATTCAAGAAATTCATCGCACGCGGCAACGTGCTTGA
TCTGGCGGTGGGCGTGATCATCGGCGGTGCCTTCGGCAAGATCGTCACCTCGCTGACCGAAAGCGTCATCATGCCGGTCG
TCGGGTGGCTGACCGGCGGGGTCGACTTCACCCGCTATTTCGTGCGGCTGGGCCCGGTGCCCGCCGACTTCAAGGGCGAT
CCGACAAGCTATGCCGAGCTGAAGGCCGCGGGCGTGCCGATGATCGGCTATGGCGATTTCATCACCCAGGCGGTGAATTT
CGTGATCGTGGCCTTCATCATCTTCCTCATCGTGAAGGCGGTGAACCGGATGTTCGAAAAGCCCGAGGAAGCACCGGCGG
CGCCTTCCGGCCCGACCGAGGTCGAACTGCTGGCCGAAATCCGGGACGCGCTGAAGGCCAAGGGCTGA

Upstream 100 bases:

>100_bases
CATAGCGCTGGCTGGTCCCGGTTCAAGGGTTCGTCCATGGCGCAACCGATTGTGACTTGTCGCGTTACCCGCGCGATGGC
AACCATGATGGAAGGGACGC

Downstream 100 bases:

>100_bases
GCGCGACCGAAACGCCGGATGAGCAGGGGTGGGGCATTCCCACCCCTTTTCATTTGGCGCGCGCTCCCTATATGGGGTCG
TGCCGGTTCAGGCCGGCTAT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MAHEVKGEILGMLGEFKKFIARGNVLDLAVGVIIGGAFGKIVTSLTESVIMPVVGWLTGGVDFTRYFVRLGPVPADFKGD
PTSYAELKAAGVPMIGYGDFITQAVNFVIVAFIIFLIVKAVNRMFEKPEEAPAAPSGPTEVELLAEIRDALKAKG

Sequences:

>Translated_155_residues
MAHEVKGEILGMLGEFKKFIARGNVLDLAVGVIIGGAFGKIVTSLTESVIMPVVGWLTGGVDFTRYFVRLGPVPADFKGD
PTSYAELKAAGVPMIGYGDFITQAVNFVIVAFIIFLIVKAVNRMFEKPEEAPAAPSGPTEVELLAEIRDALKAKG
>Mature_154_residues
AHEVKGEILGMLGEFKKFIARGNVLDLAVGVIIGGAFGKIVTSLTESVIMPVVGWLTGGVDFTRYFVRLGPVPADFKGDP
TSYAELKAAGVPMIGYGDFITQAVNFVIVAFIIFLIVKAVNRMFEKPEEAPAAPSGPTEVELLAEIRDALKAKG

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=146, Percent_Identity=45.2054794520548, Blast_Score=116, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_NOVAD (Q2G9Z8)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496159.1
- ProteinModelPortal:   Q2G9Z8
- SMR:   Q2G9Z8
- STRING:   Q2G9Z8
- GeneID:   3917965
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0880
- NMPDR:   fig|48935.1.peg.3400
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   Q2G9Z8
- ProtClustDB:   PRK13952
- BioCyc:   NARO279238:SARO_0880-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16529; Mature: 16397

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13adb998)-; HASH(0x13dc1ff4)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHEVKGEILGMLGEFKKFIARGNVLDLAVGVIIGGAFGKIVTSLTESVIMPVVGWLTGG
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDFTRYFVRLGPVPADFKGDPTSYAELKAAGVPMIGYGDFITQAVNFVIVAFIIFLIVKA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VNRMFEKPEEAPAAPSGPTEVELLAEIRDALKAKG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
AHEVKGEILGMLGEFKKFIARGNVLDLAVGVIIGGAFGKIVTSLTESVIMPVVGWLTGG
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDFTRYFVRLGPVPADFKGDPTSYAELKAAGVPMIGYGDFITQAVNFVIVAFIIFLIVKA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VNRMFEKPEEAPAAPSGPTEVELLAEIRDALKAKG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA