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The map label for this gene is mdeA [H]

Identifier: 87198835

GI number: 87198835

Start: 865399

End: 866682

Strand: Direct

Name: mdeA [H]

Synonym: Saro_0813

Alternate gene names: 87198835

Gene position: 865399-866682 (Clockwise)

Preceding gene: 87198834

Following gene: 87198837

Centisome position: 24.3

GC content: 65.65

Gene sequence:

>1284_bases
ATGACGCAGAAGCTTGAGACGCTGACGGTCCACGCCGGGTGCGAGCCCGATCCGACCACCAAGGCGCGGATCACGCCGAT
CTACCAGACGGCAAGCTACGTGTTCGACAGTGCCGAGCACGCCGCGAACCTCTTTGCGCTGGCCGAGTTCGGCAACATCT
ACTCCCGCATCATGAACCCCACCAACGCCGCGCTGGAAGCAAAGATCGCGGCGCTGGAAGGCGGTGTGGCGGCGTTGGGC
GTGGCATCTGGCCATGCCGCGCAGTTCATCGTGTTCCACACCCTGATGGAGCCGGGCTGCGAGATCGTGGCGGCCAAGAA
GCTCTATGGCGGCACGCTCAACCAGTTCGCGCATGGCTTTGCCAAGTTCGGCTGGAAGGCTGTCTTCGTCGATGCCGACG
ATCCGGCCGCGGTTGCCGCAGCGATCACCGACAAGACGCGCGGCGTGTTCATCGAAAGCCTCGCCAACCCCGGCGGCGTG
GTGCAGGACATCGCCGCGATTGCCGAGGTTGCGCATGCCGCCGGTGTGCCGCTGATCGTCGACAACACGATGGCCAGCCC
GGCGCTGTGCCGCCCGATCGAACATGGCGCGGACATCGTCGTCCATTCGTGCACCAAGTTCCTCAATGGGCATGGCAATT
CCATCGGTGGCCTGATCGTGGACGCGGGCAGGTTCGACTGGGCGGCGAGTGACAAGTTCCCCTCGCTGTCGCAGCCCAAC
CCGAGCTATCACGGCGCGGTGCTGACCGAGGCGCTGAAGCCTGTCGGGCCGATCGCGTTCATCATCGGCTGCCGCGTGCT
GGGGCTGCGCGATCTTGGCCCCGCGATGGCGCCAATGAACGCATTCCTGACGCTGACAGGCATGGAAACGCTGGCGCTGC
GGATCGAGCGCCACTGCGCCAACGCGCTGCACCTGGCGCAGTGGTTGCAGAACCATCCGAAGGTTGCCTGGGTAAGCTAT
GCGGGCCTGCCCGAAGATCCGTATCACCAGTTGGCGAAGCAGTACCTCGGCGGCAAGGGCGGCGCGGTGTTCACCTTCGG
CGTGAAGGGCGGTTACGAGGCGGGCGTGAAGCTTGTCCAGTCGGTCAAGCTGTTCAGCCACCTCGCCAACATCGGTGACA
CCCGCTCGCTGATCATCCACCCTGCCTCGACCACCCACAGCCAGCTCGGCGAGGAAGAACTGGTAGCGGCAGGCGCCGGG
CCGGACACGGTGCGCGTCTCGGTCGGCATCGAGCACATCGACGACATCGTCGCCGACCTCGCGCAGGCGCTGGAGCTGGT
GTGA

Upstream 100 bases:

>100_bases
GGTCGTTTAAAACAAATGACGCAGAGCCGGAACTCAAGCGTCTCGGTTTCAGCGTGATCAAGCGCTAGTCATTGACCGAC
TTGATCGAGGGAGAGATGTT

Downstream 100 bases:

>100_bases
TCTTGGCAGCTTCGCCTTGCCTTGTGGATGGTCGTCACCCTGAACTTGTTTCAGGGTCCATCCCTCGGCGTGCGCGGAGC
TGGCGGGCCAAGACCGAGCC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTQKLETLTVHAGCEPDPTTKARITPIYQTASYVFDSAEHAANLFALAEFGNIYSRIMNPTNAALEAKIAALEGGVAALG
VASGHAAQFIVFHTLMEPGCEIVAAKKLYGGTLNQFAHGFAKFGWKAVFVDADDPAAVAAAITDKTRGVFIESLANPGGV
VQDIAAIAEVAHAAGVPLIVDNTMASPALCRPIEHGADIVVHSCTKFLNGHGNSIGGLIVDAGRFDWAASDKFPSLSQPN
PSYHGAVLTEALKPVGPIAFIIGCRVLGLRDLGPAMAPMNAFLTLTGMETLALRIERHCANALHLAQWLQNHPKVAWVSY
AGLPEDPYHQLAKQYLGGKGGAVFTFGVKGGYEAGVKLVQSVKLFSHLANIGDTRSLIIHPASTTHSQLGEEELVAAGAG
PDTVRVSVGIEHIDDIVADLAQALELV

Sequences:

>Translated_427_residues
MTQKLETLTVHAGCEPDPTTKARITPIYQTASYVFDSAEHAANLFALAEFGNIYSRIMNPTNAALEAKIAALEGGVAALG
VASGHAAQFIVFHTLMEPGCEIVAAKKLYGGTLNQFAHGFAKFGWKAVFVDADDPAAVAAAITDKTRGVFIESLANPGGV
VQDIAAIAEVAHAAGVPLIVDNTMASPALCRPIEHGADIVVHSCTKFLNGHGNSIGGLIVDAGRFDWAASDKFPSLSQPN
PSYHGAVLTEALKPVGPIAFIIGCRVLGLRDLGPAMAPMNAFLTLTGMETLALRIERHCANALHLAQWLQNHPKVAWVSY
AGLPEDPYHQLAKQYLGGKGGAVFTFGVKGGYEAGVKLVQSVKLFSHLANIGDTRSLIIHPASTTHSQLGEEELVAAGAG
PDTVRVSVGIEHIDDIVADLAQALELV
>Mature_426_residues
TQKLETLTVHAGCEPDPTTKARITPIYQTASYVFDSAEHAANLFALAEFGNIYSRIMNPTNAALEAKIAALEGGVAALGV
ASGHAAQFIVFHTLMEPGCEIVAAKKLYGGTLNQFAHGFAKFGWKAVFVDADDPAAVAAAITDKTRGVFIESLANPGGVV
QDIAAIAEVAHAAGVPLIVDNTMASPALCRPIEHGADIVVHSCTKFLNGHGNSIGGLIVDAGRFDWAASDKFPSLSQPNP
SYHGAVLTEALKPVGPIAFIIGCRVLGLRDLGPAMAPMNAFLTLTGMETLALRIERHCANALHLAQWLQNHPKVAWVSYA
GLPEDPYHQLAKQYLGGKGGAVFTFGVKGGYEAGVKLVQSVKLFSHLANIGDTRSLIIHPASTTHSQLGEEELVAAGAGP
DTVRVSVGIEHIDDIVADLAQALELV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=427, Percent_Identity=32.7868852459016, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI299473759, Length=427, Percent_Identity=30.2107728337237, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI25453487, Length=426, Percent_Identity=28.6384976525822, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1790375, Length=421, Percent_Identity=31.8289786223278, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=26.046511627907, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=442, Percent_Identity=32.579185520362, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=425, Percent_Identity=30.3529411764706, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=30.188679245283, Blast_Score=151, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6323333, Length=441, Percent_Identity=46.7120181405896, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319307, Length=430, Percent_Identity=32.7906976744186, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=446, Percent_Identity=27.5784753363229, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=432, Percent_Identity=25.2314814814815, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321170, Length=360, Percent_Identity=26.3888888888889, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922554, Length=426, Percent_Identity=31.4553990610329, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 44860; Mature: 44729

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKLETLTVHAGCEPDPTTKARITPIYQTASYVFDSAEHAANLFALAEFGNIYSRIMNP
CCCCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCC
TNAALEAKIAALEGGVAALGVASGHAAQFIVFHTLMEPGCEIVAAKKLYGGTLNQFAHGF
CCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEHHHHHCCCHHHHHHHH
AKFGWKAVFVDADDPAAVAAAITDKTRGVFIESLANPGGVVQDIAAIAEVAHAAGVPLIV
HHCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
DNTMASPALCRPIEHGADIVVHSCTKFLNGHGNSIGGLIVDAGRFDWAASDKFPSLSQPN
ECCCCCCHHHCHHHCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
PSYHGAVLTEALKPVGPIAFIIGCRVLGLRDLGPAMAPMNAFLTLTGMETLALRIERHCA
CCCCHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCHHCCCHHEEEECCHHHHHHHHHHHHH
NALHLAQWLQNHPKVAWVSYAGLPEDPYHQLAKQYLGGKGGAVFTFGVKGGYEAGVKLVQ
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
SVKLFSHLANIGDTRSLIIHPASTTHSQLGEEELVAAGAGPDTVRVSVGIEHIDDIVADL
HHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHEECCCCCCEEEEEECHHHHHHHHHHH
AQALELV
HHHHHCC
>Mature Secondary Structure 
TQKLETLTVHAGCEPDPTTKARITPIYQTASYVFDSAEHAANLFALAEFGNIYSRIMNP
CCCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCC
TNAALEAKIAALEGGVAALGVASGHAAQFIVFHTLMEPGCEIVAAKKLYGGTLNQFAHGF
CCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEHHHHHCCCHHHHHHHH
AKFGWKAVFVDADDPAAVAAAITDKTRGVFIESLANPGGVVQDIAAIAEVAHAAGVPLIV
HHCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
DNTMASPALCRPIEHGADIVVHSCTKFLNGHGNSIGGLIVDAGRFDWAASDKFPSLSQPN
ECCCCCCHHHCHHHCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
PSYHGAVLTEALKPVGPIAFIIGCRVLGLRDLGPAMAPMNAFLTLTGMETLALRIERHCA
CCCCHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCHHCCCHHEEEECCHHHHHHHHHHHHH
NALHLAQWLQNHPKVAWVSYAGLPEDPYHQLAKQYLGGKGGAVFTFGVKGGYEAGVKLVQ
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
SVKLFSHLANIGDTRSLIIHPASTTHSQLGEEELVAAGAGPDTVRVSVGIEHIDDIVADL
HHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHEECCCCCCEEEEEECHHHHHHHHHHH
AQALELV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]