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The map label for this gene is dnaN [H]

Identifier: 87198663

GI number: 87198663

Start: 677953

End: 679200

Strand: Direct

Name: dnaN [H]

Synonym: Saro_0639

Alternate gene names: 87198663

Gene position: 677953-679200 (Clockwise)

Preceding gene: 87198662

Following gene: 87198664

Centisome position: 19.04

GC content: 65.14

Gene sequence:

>1248_bases
GTGATCGCAACCGACAAGCGCAAGCCCGCCTTCCGGATTGCGGCGAAGGCTATGGCCAAGGCATTGGCCGACGTCGTGGA
AGTGACACCGGCGGGCGGGAAGGACACCATTCCTATCCTGAACACCGTACGCATGGAGGCAAGTGACGGCGTTCTGACGC
TGACGGGTTGCGACCTGGACCAGTGGATCATTCGTTCGCTGCCGACGTCGGACCGTGAGCCCAATTCGGCGGAATGGCTG
GCATCGATCCGGCCCTTCGCAACATGCGTCCCGGCCCGGGCGCTGCTGGCGATCGTGAAGCAGATCGATGCCGACGCCAT
GGTGACGGTGGCAGGCCCTACGGACAAGGAAAGCCGGGTAACCATTACCGCCGGTCGAAGCCGGTTTCGCGTGGCGTGCC
TGCCGGTAGACGACTTTCCGTTCCCGACGCGCCCCGACTTCGAGCATAGCTTCAGCATCGCCGCTGGCGCGCTCCGCGAC
GTGTTCGCCCGTGTAGAGCATGCGATCTCGACCGAAGAGACGCGCTACTACCTCAACGGCGTGTACATGCATCCGCAAGA
TCTGGACCAGCGCTTTGCCGCCACGGACGGTCACAGGCTTGCCCGCCTGGTCCAGGACGGCCCCGACGGCGCGGCATCCT
TTCCTGCCGTGATCATCGGCAGGCGCACGGTGGCCGTACTGGACAAGCTGCTCGATGCCGCGGGCAAGGTCGATACTTCG
ACGAGCGACGACGCCGACAAGGCATGCAGCCGGCCGATGGTCGAGGTGGATTCCGACAGCAACGGCAGGGTGATCTACTG
GGCCATGCCGGCACCCGATGGCGGAGAGGTCACGCTGATCGCCAAGACGGTGGATGGCACGTTTCCGGACTATGCACGGG
TGATACCCTCGGCCCCCTCGAGCTTCCTGACGGTCGATCGTGAAGCGTTGCTGGCCGCGATCAAGCGGGTGGCGGCGGTC
TGTTCCGACAAGACGCGTGCGGTGAAGCTCGAGCTGGATGGCGCGGAGAAGGCGATCGTCTCCACCGCCTCGCCCGAGAT
TGGCGACGCGATGGAAGAGATCGCCTGCGAGTATGCCGGCGAGCCGCTGACCATCGGCTTCAACGGGGACTACTGGCGCA
GCTGCCTGAGCGCGGTGGCGACCGACAAGGTCCTGATGAAGTTCACCGACGCAGGTGGGCCGTGCCGCATCGAGGCGGAA
GGTGGCGAGTCTGCGCTGGTCCAAGTGCTGATGCCCTTGCGGGTCTGA

Upstream 100 bases:

>100_bases
AGGGAGCGGATACCCATGCCCGCGCCAAATGGGCGGCCGAGCAGATCGCCATCGAGATCGAAGCCTTCGAGCAGGGTCTT
CATGAAGGGAGGGCTGCGCA

Downstream 100 bases:

>100_bases
TTGGCGGGAGCCTGACATGAGCGTTCAATCCGATATCGGCCCGGCGCGCGGTGGCGTGCCGCGGTGGAAAACGTATGCCC
AGGCGCGGGAGATTGCGGCG

Product: DNA polymerase III subunit beta family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MIATDKRKPAFRIAAKAMAKALADVVEVTPAGGKDTIPILNTVRMEASDGVLTLTGCDLDQWIIRSLPTSDREPNSAEWL
ASIRPFATCVPARALLAIVKQIDADAMVTVAGPTDKESRVTITAGRSRFRVACLPVDDFPFPTRPDFEHSFSIAAGALRD
VFARVEHAISTEETRYYLNGVYMHPQDLDQRFAATDGHRLARLVQDGPDGAASFPAVIIGRRTVAVLDKLLDAAGKVDTS
TSDDADKACSRPMVEVDSDSNGRVIYWAMPAPDGGEVTLIAKTVDGTFPDYARVIPSAPSSFLTVDREALLAAIKRVAAV
CSDKTRAVKLELDGAEKAIVSTASPEIGDAMEEIACEYAGEPLTIGFNGDYWRSCLSAVATDKVLMKFTDAGGPCRIEAE
GGESALVQVLMPLRV

Sequences:

>Translated_415_residues
MIATDKRKPAFRIAAKAMAKALADVVEVTPAGGKDTIPILNTVRMEASDGVLTLTGCDLDQWIIRSLPTSDREPNSAEWL
ASIRPFATCVPARALLAIVKQIDADAMVTVAGPTDKESRVTITAGRSRFRVACLPVDDFPFPTRPDFEHSFSIAAGALRD
VFARVEHAISTEETRYYLNGVYMHPQDLDQRFAATDGHRLARLVQDGPDGAASFPAVIIGRRTVAVLDKLLDAAGKVDTS
TSDDADKACSRPMVEVDSDSNGRVIYWAMPAPDGGEVTLIAKTVDGTFPDYARVIPSAPSSFLTVDREALLAAIKRVAAV
CSDKTRAVKLELDGAEKAIVSTASPEIGDAMEEIACEYAGEPLTIGFNGDYWRSCLSAVATDKVLMKFTDAGGPCRIEAE
GGESALVQVLMPLRV
>Mature_415_residues
MIATDKRKPAFRIAAKAMAKALADVVEVTPAGGKDTIPILNTVRMEASDGVLTLTGCDLDQWIIRSLPTSDREPNSAEWL
ASIRPFATCVPARALLAIVKQIDADAMVTVAGPTDKESRVTITAGRSRFRVACLPVDDFPFPTRPDFEHSFSIAAGALRD
VFARVEHAISTEETRYYLNGVYMHPQDLDQRFAATDGHRLARLVQDGPDGAASFPAVIIGRRTVAVLDKLLDAAGKVDTS
TSDDADKACSRPMVEVDSDSNGRVIYWAMPAPDGGEVTLIAKTVDGTFPDYARVIPSAPSSFLTVDREALLAAIKRVAAV
CSDKTRAVKLELDGAEKAIVSTASPEIGDAMEEIACEYAGEPLTIGFNGDYWRSCLSAVATDKVLMKFTDAGGPCRIEAE
GGESALVQVLMPLRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=408, Percent_Identity=29.656862745098, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 44477; Mature: 44477

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATDKRKPAFRIAAKAMAKALADVVEVTPAGGKDTIPILNTVRMEASDGVLTLTGCDLD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEECCCCEEEEECCCHH
QWIIRSLPTSDREPNSAEWLASIRPFATCVPARALLAIVKQIDADAMVTVAGPTDKESRV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEE
TITAGRSRFRVACLPVDDFPFPTRPDFEHSFSIAAGALRDVFARVEHAISTEETRYYLNG
EEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VYMHPQDLDQRFAATDGHRLARLVQDGPDGAASFPAVIIGRRTVAVLDKLLDAAGKVDTS
EEECHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
TSDDADKACSRPMVEVDSDSNGRVIYWAMPAPDGGEVTLIAKTVDGTFPDYARVIPSAPS
CCCCHHHHHCCCCEEECCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCHHHHHCCCCCC
SFLTVDREALLAAIKRVAAVCSDKTRAVKLELDGAEKAIVSTASPEIGDAMEEIACEYAG
HHEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCC
EPLTIGFNGDYWRSCLSAVATDKVLMKFTDAGGPCRIEAEGGESALVQVLMPLRV
CCEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIATDKRKPAFRIAAKAMAKALADVVEVTPAGGKDTIPILNTVRMEASDGVLTLTGCDLD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEECCCCEEEEECCCHH
QWIIRSLPTSDREPNSAEWLASIRPFATCVPARALLAIVKQIDADAMVTVAGPTDKESRV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEE
TITAGRSRFRVACLPVDDFPFPTRPDFEHSFSIAAGALRDVFARVEHAISTEETRYYLNG
EEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VYMHPQDLDQRFAATDGHRLARLVQDGPDGAASFPAVIIGRRTVAVLDKLLDAAGKVDTS
EEECHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
TSDDADKACSRPMVEVDSDSNGRVIYWAMPAPDGGEVTLIAKTVDGTFPDYARVIPSAPS
CCCCHHHHHCCCCEEECCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCHHHHHCCCCCC
SFLTVDREALLAAIKRVAAVCSDKTRAVKLELDGAEKAIVSTASPEIGDAMEEIACEYAG
HHEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCC
EPLTIGFNGDYWRSCLSAVATDKVLMKFTDAGGPCRIEAEGGESALVQVLMPLRV
CCEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]