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The map label for this gene is ispG

Identifier: 87198442

GI number: 87198442

Start: 455622

End: 456746

Strand: Direct

Name: ispG

Synonym: Saro_0417

Alternate gene names: 87198442

Gene position: 455622-456746 (Clockwise)

Preceding gene: 87198441

Following gene: 87198444

Centisome position: 12.79

GC content: 66.04

Gene sequence:

>1125_bases
ATGTCATCGGTACGTCCCTGGCGCGATATCGCGCGTCGCAAGAGCCGCCAGATCATGGTCGGCACGGTCCCCGTCGGCGG
CGATGCCCCGATCACCGTGCAGACCATGACCAACACCCCGACGTCCGACGCCGTCGCCACGATCGACCAGATCCGTCGCT
GCGAGGAAGCGGGCGCCGATCTTATCCGCGTGTCCTGTCCCGACGTGGAAAGCACAGCGGCCTTCCGCCAGATCGCCCGG
GCCGCCCGGGTTCCGCTGATCGCGGACATCCACTTCCACTACAAGCGCGCGCTCGAAGCGGCCGATGCGGGGGCCGCGTG
CCTGCGCATCAATCCGGGCAACATCGGCAGCAGCGACCGCGTGGCCGAAGTGGTCCGCGCCGCCAAGGCCAACGGCTGCG
CGATCCGCATCGGCGTCAACGCTGGCAGCCTCGAGAAAGACCTGCTCGAAAAGTACGGCGAGCCCTGTCCCGAAGCGCTC
GTCGAATCCGCGCTCGACCATATCAAGCTGCTGCAGGACCACGATTTCCACGAATACAAGGTGGCGGTTAAGGCCTCCGA
CGTGTTCCTCGCCGTCGCCGCCTACATGGGCCTTGCAGAAGCGGTCGATTGCCCGCTGCATCTTGGCATTACCGAGGCAG
GCGGGCTGATCGGCGGGACGGTGAAATCGTCCGTCGGCATCGGCAACCTGCTCTGGGCCGGCATCGGCGACACCTTGCGC
GTCTCGCTTTCGGCCGAACCGGAAGAGGAAGTGCGCGTCGGGTTCGAGATCCTCAAGACGCTGGGCCTGCGCACGCGCGG
CGTCCGCGTCGTGTCGTGTCCGTCCTGCGCTCGGCAGGGTTTCGACGTGATCCGGACCGTGGAGGCGCTGGAAAAGCGGC
TGACGCACATCAAGACGCCGATCTCGCTTTCCGTACTGGGCTGCGTCGTCAATGGACCGGGCGAAGCCCGCGAGACCGAT
ATCGGCCTCACCGGCGGCGGCAACGGCAAGCACATGGTCTATCTTTCGGGCGTGACCGACCACCACGTCCAGTCGGAGGA
CATGCTAGACCACATCGTCTCGCTGGTCGAACAGAAGGCTGCGGAAATGGAAGCCGCTGCCGCAGAAGCGGAAGCGGCAG
CCTGA

Upstream 100 bases:

>100_bases
CCCCGCCGAGGCCCGCAACCCCGGTCCCATGTGCAGGTAGGTGCTTTCAGGTGCCTTTAGGTGCCTTTCAGGTCGCTGCG
TAAATCCCTATAGAGGCCCC

Downstream 100 bases:

>100_bases
ATCAGGTCCCGGTTGCGAGCGCCAGGGACAGGACCACGGCGTTCGAGGCGACGTGGGTGAGGATCGCGGCCACCAGGCCG
ATCCTGACGCGGATGTAGCC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSSVRPWRDIARRKSRQIMVGTVPVGGDAPITVQTMTNTPTSDAVATIDQIRRCEEAGADLIRVSCPDVESTAAFRQIAR
AARVPLIADIHFHYKRALEAADAGAACLRINPGNIGSSDRVAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEAL
VESALDHIKLLQDHDFHEYKVAVKASDVFLAVAAYMGLAEAVDCPLHLGITEAGGLIGGTVKSSVGIGNLLWAGIGDTLR
VSLSAEPEEEVRVGFEILKTLGLRTRGVRVVSCPSCARQGFDVIRTVEALEKRLTHIKTPISLSVLGCVVNGPGEARETD
IGLTGGGNGKHMVYLSGVTDHHVQSEDMLDHIVSLVEQKAAEMEAAAAEAEAAA

Sequences:

>Translated_374_residues
MSSVRPWRDIARRKSRQIMVGTVPVGGDAPITVQTMTNTPTSDAVATIDQIRRCEEAGADLIRVSCPDVESTAAFRQIAR
AARVPLIADIHFHYKRALEAADAGAACLRINPGNIGSSDRVAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEAL
VESALDHIKLLQDHDFHEYKVAVKASDVFLAVAAYMGLAEAVDCPLHLGITEAGGLIGGTVKSSVGIGNLLWAGIGDTLR
VSLSAEPEEEVRVGFEILKTLGLRTRGVRVVSCPSCARQGFDVIRTVEALEKRLTHIKTPISLSVLGCVVNGPGEARETD
IGLTGGGNGKHMVYLSGVTDHHVQSEDMLDHIVSLVEQKAAEMEAAAAEAEAAA
>Mature_373_residues
SSVRPWRDIARRKSRQIMVGTVPVGGDAPITVQTMTNTPTSDAVATIDQIRRCEEAGADLIRVSCPDVESTAAFRQIARA
ARVPLIADIHFHYKRALEAADAGAACLRINPGNIGSSDRVAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEALV
ESALDHIKLLQDHDFHEYKVAVKASDVFLAVAAYMGLAEAVDCPLHLGITEAGGLIGGTVKSSVGIGNLLWAGIGDTLRV
SLSAEPEEEVRVGFEILKTLGLRTRGVRVVSCPSCARQGFDVIRTVEALEKRLTHIKTPISLSVLGCVVNGPGEARETDI
GLTGGGNGKHMVYLSGVTDHHVQSEDMLDHIVSLVEQKAAEMEAAAAEAEAAA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=357, Percent_Identity=55.1820728291317, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016425
- InterPro:   IPR004588
- InterPro:   IPR011060 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 39625; Mature: 39494

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVRPWRDIARRKSRQIMVGTVPVGGDAPITVQTMTNTPTSDAVATIDQIRRCEEAGAD
CCCCCHHHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
LIRVSCPDVESTAAFRQIARAARVPLIADIHFHYKRALEAADAGAACLRINPGNIGSSDR
EEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
VAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEALVESALDHIKLLQDHDFHEYK
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VAVKASDVFLAVAAYMGLAEAVDCPLHLGITEAGGLIGGTVKSSVGIGNLLWAGIGDTLR
EEEEHHHHHHHHHHHHHHHHHHCCCEEECEECCCCCCCCHHHHCCCCCHHHHHCCCCEEE
VSLSAEPEEEVRVGFEILKTLGLRTRGVRVVSCPSCARQGFDVIRTVEALEKRLTHIKTP
EEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
ISLSVLGCVVNGPGEARETDIGLTGGGNGKHMVYLSGVTDHHVQSEDMLDHIVSLVEQKA
CEEEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
AEMEAAAAEAEAAA
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SSVRPWRDIARRKSRQIMVGTVPVGGDAPITVQTMTNTPTSDAVATIDQIRRCEEAGAD
CCCCHHHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
LIRVSCPDVESTAAFRQIARAARVPLIADIHFHYKRALEAADAGAACLRINPGNIGSSDR
EEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
VAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEALVESALDHIKLLQDHDFHEYK
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VAVKASDVFLAVAAYMGLAEAVDCPLHLGITEAGGLIGGTVKSSVGIGNLLWAGIGDTLR
EEEEHHHHHHHHHHHHHHHHHHCCCEEECEECCCCCCCCHHHHCCCCCHHHHHCCCCEEE
VSLSAEPEEEVRVGFEILKTLGLRTRGVRVVSCPSCARQGFDVIRTVEALEKRLTHIKTP
EEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
ISLSVLGCVVNGPGEARETDIGLTGGGNGKHMVYLSGVTDHHVQSEDMLDHIVSLVEQKA
CEEEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
AEMEAAAAEAEAAA
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA