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The map label for this gene is braD [H]

Identifier: 86751759

GI number: 86751759

Start: 5272261

End: 5273133

Strand: Reverse

Name: braD [H]

Synonym: RPB_4661

Alternate gene names: 86751759

Gene position: 5273133-5272261 (Counterclockwise)

Preceding gene: 86751760

Following gene: 86751758

Centisome position: 98.9

GC content: 64.15

Gene sequence:

>873_bases
ATGGACCTGATCACCTTCGGCGTGCAGCTCCTGAATGCCGTCCAATACGGCATGGTGCTGTTCCTGGTCGCCAGCGGGCT
AACGCTGGTGTTCGGCATTCTCGGCGTGATCAATCTCGCGCATGGCGCGTTCTACATGCTCGGCGCGTATCTGGCCTATT
GGATCGCGCTCATGACCGGCAACTTTCTGGTCGCGCTGGTCGGCGGCGTCGCGATCGCTTTCGTGCTCGGGCTGGCGCTG
GAAAGCGTATTCATCCGCTGGCTGTACGGCCGCGATCATCTGGCGCAGGTGCTGCTGTCGTTCGGGCTGATCCTGGTGAT
CGACGAGGCGCGGCAACTGCTGTTCGGCAAGGACGTGCATTCGGTGGCGCCGCCGGACTGGTTGTCCGGCTCGCTCCAGC
TCACCGACAATCTGTCTTATCCAGTCTATCGCCTCGCGATATGCGTGTTCTGTCTGGCGGTCGCGGCGCTGATCTTTTTC
GTGATCACCCGGACCAAGATCGGCATGATCGTCCGCGCCGGCGCCGAGAACCGCGAGATGACCCGGGTGCTGGGCATCGA
CTACGACAAGGTCAACCGCCTCGTCTTCGCCACCGGCATTGCGCTGGCCGCCCTCGGCGGCATCGTCACCGCGCCGATGT
CGACGGTTTATCCGGGCATGGGTGACGGCATGCTGATCCTGAGCTTCGTGGTCGTGGTGCTCGGCGGCATCGGCTCGGTT
GCCGGCGCCGCGGTCGGCGCGCTGCTGATCGGCTTCACCGACACCTTCGGCAAGGTGTTCTTCCCGAGCGTGTCCGGCAT
GCTGATCTATCTGCTGATGGCGATCGTCCTGCTGTGGCGCCCGAACGGCATTCTGGGCCGGCGGGAGGTGTGA

Upstream 100 bases:

>100_bases
TACTCCCTGACGGTGGTTATGGATTCCGGGTTCGCGAGTTGCGCTCGCGCCCCGGAATGACGAACGCGAGCGGAGTAATC
GTGCAGTTAGGCCTTAAGCC

Downstream 100 bases:

>100_bases
GATGAAGCGTCTCTCCGCCCCCGCCGTCATGCCCGGGCTCGTCCCGGGCATCCACGAATCGACGCCTGCTTCGACGCAAG
AACGTGGATGGCCGGGACGA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDLITFGVQLLNAVQYGMVLFLVASGLTLVFGILGVINLAHGAFYMLGAYLAYWIALMTGNFLVALVGGVAIAFVLGLAL
ESVFIRWLYGRDHLAQVLLSFGLILVIDEARQLLFGKDVHSVAPPDWLSGSLQLTDNLSYPVYRLAICVFCLAVAALIFF
VITRTKIGMIVRAGAENREMTRVLGIDYDKVNRLVFATGIALAALGGIVTAPMSTVYPGMGDGMLILSFVVVVLGGIGSV
AGAAVGALLIGFTDTFGKVFFPSVSGMLIYLLMAIVLLWRPNGILGRREV

Sequences:

>Translated_290_residues
MDLITFGVQLLNAVQYGMVLFLVASGLTLVFGILGVINLAHGAFYMLGAYLAYWIALMTGNFLVALVGGVAIAFVLGLAL
ESVFIRWLYGRDHLAQVLLSFGLILVIDEARQLLFGKDVHSVAPPDWLSGSLQLTDNLSYPVYRLAICVFCLAVAALIFF
VITRTKIGMIVRAGAENREMTRVLGIDYDKVNRLVFATGIALAALGGIVTAPMSTVYPGMGDGMLILSFVVVVLGGIGSV
AGAAVGALLIGFTDTFGKVFFPSVSGMLIYLLMAIVLLWRPNGILGRREV
>Mature_290_residues
MDLITFGVQLLNAVQYGMVLFLVASGLTLVFGILGVINLAHGAFYMLGAYLAYWIALMTGNFLVALVGGVAIAFVLGLAL
ESVFIRWLYGRDHLAQVLLSFGLILVIDEARQLLFGKDVHSVAPPDWLSGSLQLTDNLSYPVYRLAICVFCLAVAALIFF
VITRTKIGMIVRAGAENREMTRVLGIDYDKVNRLVFATGIALAALGGIVTAPMSTVYPGMGDGMLILSFVVVVLGGIGSV
AGAAVGALLIGFTDTFGKVFFPSVSGMLIYLLMAIVLLWRPNGILGRREV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=300, Percent_Identity=34, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31016; Mature: 31016

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00195 GLUTAREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]