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The map label for this gene is livM [H]

Identifier: 86751758

GI number: 86751758

Start: 5271121

End: 5272152

Strand: Reverse

Name: livM [H]

Synonym: RPB_4660

Alternate gene names: 86751758

Gene position: 5272152-5271121 (Counterclockwise)

Preceding gene: 86751759

Following gene: 86751757

Centisome position: 98.88

GC content: 65.6

Gene sequence:

>1032_bases
ATGACGAACGAAAGGACGAGCCTGATCCGATCAAGCGCACTGCCGGCAATCTGTCTGATCGCCGCACTCGCGCTGCCCTT
CGTCGTGCCGGTGTACTACGTACAATTCGCCAGCAAGGCGCTGATTCTCGGCGTGCTGGCGATGGCGCTCAATCTGGTGG
TCGGGCAGGGCGGGCTGGTGTCGCTGTGCCATGCCGCATTCTTTGGCCTGGCCGGCTACGTGCTGGCGCTGATGTCACCG
AAATACGACGCGGCGTCGTTGCTCCTGACGTGGCCTGCCGCAGTGCTGGCGGCGAGTTTTGCCGCACTGGTGATCGGGGC
GTTGGCGCTGCGCACCCGCGGCGTATACTTCATCATGGTGACGCTCGCTTTCGGTGAAATGCTGTTCTACCTGTTTCACG
ATGCCGACTTCGCCGGCGGCTCCGATGGCGCCTTCATCAACGTCAAGCCGGAATTGGTGATCGCGGGCGTCCGTCTGCTC
GATCTCGACAAGCCGCTGACGTTCTACTTTCTCGTGCTCGGCGTCACCGTGGCGGCGATCGCGTTGCTGGTGACGGTGGT
GCGCTCGCCGTTCGGCCACGCGCTGGCGGCGGCGCGCGACAATGAGCGCCGCGCCCGCGCGCTCGGCTTCCCGATCTTCC
GCATTCGCCTCATCGCCTTCGTGCTGTCCGGTGCGCTCAGTGGCGTCGCCGGCTATCTCGCCGCGGTGCAGTTCGGCTTC
GTCGCACCGCAGATGCTGGGCTGGCATCAGTCGGCGACGGTGCTGGTGATGGTGCTGATCGGTGGGCTCGGCACCGTGAC
CGGGCCGCTGATCGGTGCGCTGGTGCTGCTCGGGCTCGAGGAAGTGCTGAAAGCGAGCTTCGAGCACTGGAAACTGATCG
AGGGCGTGATCGTGATCGCAATCGTACTGCTGCTGCCGAACGGCGTCCGCCAGCTTTGGCCGATGCTCGTGGGCGATCGC
GAGCCGAAGTCCGTGCACGGCAAACAGACCACGCCGGCTCCGCGCCCGGCATCGGAGACCGGCCATGCCTGA

Upstream 100 bases:

>100_bases
GTCTCTCCGCCCCCGCCGTCATGCCCGGGCTCGTCCCGGGCATCCACGAATCGACGCCTGCTTCGACGCAAGAACGTGGA
TGGCCGGGACGAGCCCGGCC

Downstream 100 bases:

>100_bases
ACTCGGATTGCGCGCCGAACGGCTCGGCAAACGCTTCGGCGGCGTTCGCGCGGTCGCCGACGTGTCGGTCGACATCAAGC
ATGGCGAAATCCACGCGGTG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTNERTSLIRSSALPAICLIAALALPFVVPVYYVQFASKALILGVLAMALNLVVGQGGLVSLCHAAFFGLAGYVLALMSP
KYDAASLLLTWPAAVLAASFAALVIGALALRTRGVYFIMVTLAFGEMLFYLFHDADFAGGSDGAFINVKPELVIAGVRLL
DLDKPLTFYFLVLGVTVAAIALLVTVVRSPFGHALAAARDNERRARALGFPIFRIRLIAFVLSGALSGVAGYLAAVQFGF
VAPQMLGWHQSATVLVMVLIGGLGTVTGPLIGALVLLGLEEVLKASFEHWKLIEGVIVIAIVLLLPNGVRQLWPMLVGDR
EPKSVHGKQTTPAPRPASETGHA

Sequences:

>Translated_343_residues
MTNERTSLIRSSALPAICLIAALALPFVVPVYYVQFASKALILGVLAMALNLVVGQGGLVSLCHAAFFGLAGYVLALMSP
KYDAASLLLTWPAAVLAASFAALVIGALALRTRGVYFIMVTLAFGEMLFYLFHDADFAGGSDGAFINVKPELVIAGVRLL
DLDKPLTFYFLVLGVTVAAIALLVTVVRSPFGHALAAARDNERRARALGFPIFRIRLIAFVLSGALSGVAGYLAAVQFGF
VAPQMLGWHQSATVLVMVLIGGLGTVTGPLIGALVLLGLEEVLKASFEHWKLIEGVIVIAIVLLLPNGVRQLWPMLVGDR
EPKSVHGKQTTPAPRPASETGHA
>Mature_342_residues
TNERTSLIRSSALPAICLIAALALPFVVPVYYVQFASKALILGVLAMALNLVVGQGGLVSLCHAAFFGLAGYVLALMSPK
YDAASLLLTWPAAVLAASFAALVIGALALRTRGVYFIMVTLAFGEMLFYLFHDADFAGGSDGAFINVKPELVIAGVRLLD
LDKPLTFYFLVLGVTVAAIALLVTVVRSPFGHALAAARDNERRARALGFPIFRIRLIAFVLSGALSGVAGYLAAVQFGFV
APQMLGWHQSATVLVMVLIGGLGTVTGPLIGALVLLGLEEVLKASFEHWKLIEGVIVIAIVLLLPNGVRQLWPMLVGDRE
PKSVHGKQTTPAPRPASETGHA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=303, Percent_Identity=30.03300330033, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36256; Mature: 36125

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNERTSLIRSSALPAICLIAALALPFVVPVYYVQFASKALILGVLAMALNLVVGQGGLV
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SLCHAAFFGLAGYVLALMSPKYDAASLLLTWPAAVLAASFAALVIGALALRTRGVYFIMV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TLAFGEMLFYLFHDADFAGGSDGAFINVKPELVIAGVRLLDLDKPLTFYFLVLGVTVAAI
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHEECHHHEECCCHHHHHHHHHHHHHHHH
ALLVTVVRSPFGHALAAARDNERRARALGFPIFRIRLIAFVLSGALSGVAGYLAAVQFGF
HHHHHHHHCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAPQMLGWHQSATVLVMVLIGGLGTVTGPLIGALVLLGLEEVLKASFEHWKLIEGVIVIA
HHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVLLLPNGVRQLWPMLVGDREPKSVHGKQTTPAPRPASETGHA
HHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TNERTSLIRSSALPAICLIAALALPFVVPVYYVQFASKALILGVLAMALNLVVGQGGLV
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SLCHAAFFGLAGYVLALMSPKYDAASLLLTWPAAVLAASFAALVIGALALRTRGVYFIMV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TLAFGEMLFYLFHDADFAGGSDGAFINVKPELVIAGVRLLDLDKPLTFYFLVLGVTVAAI
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHEECHHHEECCCHHHHHHHHHHHHHHHH
ALLVTVVRSPFGHALAAARDNERRARALGFPIFRIRLIAFVLSGALSGVAGYLAAVQFGF
HHHHHHHHCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAPQMLGWHQSATVLVMVLIGGLGTVTGPLIGALVLLGLEEVLKASFEHWKLIEGVIVIA
HHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVLLLPNGVRQLWPMLVGDREPKSVHGKQTTPAPRPASETGHA
HHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]