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The map label for this gene is actII-2 [H]

Identifier: 86751732

GI number: 86751732

Start: 5237067

End: 5238482

Strand: Reverse

Name: actII-2 [H]

Synonym: RPB_4634

Alternate gene names: 86751732

Gene position: 5238482-5237067 (Counterclockwise)

Preceding gene: 86751733

Following gene: 86751731

Centisome position: 98.25

GC content: 67.37

Gene sequence:

>1416_bases
ATGGATCAGACCCTCGCCGTCACCACGGCTTCTTCGCGCCGCTGGCGGGTGCTGGCGATCGTGGTCGCCGCGCAGTTCAT
GTTCGGCGTCGATTCCTTCATCGTCAACGTCGCGATTCCGACGATCTCCGTCGAGCTGAACGCCTCGTCGTCACAGCTCG
AGGCGGTGATCGCGATCTATCTGATCGGCTATGCGACACTGATCGTCACCGGCGGCCGGCTCGGCGATATCTACGGCACC
AAGACGGTATTCCTCGCCGGTGTGATCGGCTTCACGTTGACTTCGCTGTGGTGCGGGCTGGCGCGCTCCGGCACCGAACT
GATCCTGGCGCGGCTCGCGCAGGGCACCACCGCGGCGCTGATGGTGCCGCAGGTGCTGGCGACGCTGCACGTGCTGTTTC
CGGACGCCGCCCGCGCCAAGGCTTTCGCGATCTACGGAATTGTGCTCGGGCTCGCCGGCGCCGCCGGCTTCGCGCTCGGC
GGGCTATTGGTGACGCTCGATCTCGGCGGCTACGGCTGGCGCGCGATCTTCTTCGTCAATGGTCCGGTCGGGCTGATCAT
CTTCGCCGCGGCGGCGTGGGTGGTGCCGCAGGCGCCGCGCCGGCCGGGCACGAGGCTCGATCTGCCGGGCGCGGTGATCC
TGTTCACCGGCTTGCTGTGCGTGATCGGCCCGCTGCTGTTCGGCCGCGAAGTCGACTGGGCGGGCTGGATCTGGGTGGTG
ATGGCCGCCGGCGTCGCCATCGTGCTGATTTTCCTGCGCTACGAACGCGGTGTTGCGGCGCGCGGCGGGATGCCGCTGAT
CGATCTGGCGCTACTCGCCGACTCCGCTTTCATGCGCGGCCTCGGCGCGGTGTTCTGCTTCTTCTTCGCCAACCAGTCAT
TCTATCTGGTGGTGACGCTTTACATGCAGATGGTGCTCGAGATCCCGCCGCTTCCAGCCGGCCTGGTGTTCCTGCCATTG
GCGTTGGCCTTCGTGATTGCGGCCCGGCATTCCGGCGCGCGGGCACGGCGCCGTGGCACCCTGGTGCTGATCGAGGGCTG
CCTGCTGCAGATCGCAGGTCTCGCTCTGGTCGCGCTGACGGTCGCCATGATCGAGGCGCCGACGCCGTTCGTGCTGGCGC
TGGTGCTGATCGTGGTCGGCTACGGTCAGGGCCTGGTGATGGCGCCGTTGTCCGGCGCAGTGTTGTCGACGGTACAGGCG
GCCAGCGCCGGCTCCGGCTCCGGCCTGTACGGCACCGCCACGCAGATTTCCTCGGCCGCCGGTATCGCTGCGATCGGCTC
GCTGTATTTTTCGCTCGACCATGCGATCTCCGGGCAATTCGCGTTCCTGGTCGCATTGTCGGTGATCGCAGGCTCGGTCG
CCGGCAGCATCATCCTGCTGCACTGGATGCGGCGGGCGGCCTTGGTCCCAACGTAG

Upstream 100 bases:

>100_bases
CGATGTCCCCATTCATTCCGGTTACCTGCCGGGTAATCGACCCGATGACGACGGGCTGCGACGCTCCGGCTTCCGCAGCC
GTTTGCCCAGGATCGTCGCC

Downstream 100 bases:

>100_bases
GGATGTTTCGCTCTTGCCCGGTGCGTTAAGCCTCCGATGCCGCGCGTCGGGGGACCGCGCGGTCTATTGCTGCACGGCGT
CGATCTTTGACGGCTTCCGC

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MDQTLAVTTASSRRWRVLAIVVAAQFMFGVDSFIVNVAIPTISVELNASSSQLEAVIAIYLIGYATLIVTGGRLGDIYGT
KTVFLAGVIGFTLTSLWCGLARSGTELILARLAQGTTAALMVPQVLATLHVLFPDAARAKAFAIYGIVLGLAGAAGFALG
GLLVTLDLGGYGWRAIFFVNGPVGLIIFAAAAWVVPQAPRRPGTRLDLPGAVILFTGLLCVIGPLLFGREVDWAGWIWVV
MAAGVAIVLIFLRYERGVAARGGMPLIDLALLADSAFMRGLGAVFCFFFANQSFYLVVTLYMQMVLEIPPLPAGLVFLPL
ALAFVIAARHSGARARRRGTLVLIEGCLLQIAGLALVALTVAMIEAPTPFVLALVLIVVGYGQGLVMAPLSGAVLSTVQA
ASAGSGSGLYGTATQISSAAGIAAIGSLYFSLDHAISGQFAFLVALSVIAGSVAGSIILLHWMRRAALVPT

Sequences:

>Translated_471_residues
MDQTLAVTTASSRRWRVLAIVVAAQFMFGVDSFIVNVAIPTISVELNASSSQLEAVIAIYLIGYATLIVTGGRLGDIYGT
KTVFLAGVIGFTLTSLWCGLARSGTELILARLAQGTTAALMVPQVLATLHVLFPDAARAKAFAIYGIVLGLAGAAGFALG
GLLVTLDLGGYGWRAIFFVNGPVGLIIFAAAAWVVPQAPRRPGTRLDLPGAVILFTGLLCVIGPLLFGREVDWAGWIWVV
MAAGVAIVLIFLRYERGVAARGGMPLIDLALLADSAFMRGLGAVFCFFFANQSFYLVVTLYMQMVLEIPPLPAGLVFLPL
ALAFVIAARHSGARARRRGTLVLIEGCLLQIAGLALVALTVAMIEAPTPFVLALVLIVVGYGQGLVMAPLSGAVLSTVQA
ASAGSGSGLYGTATQISSAAGIAAIGSLYFSLDHAISGQFAFLVALSVIAGSVAGSIILLHWMRRAALVPT
>Mature_471_residues
MDQTLAVTTASSRRWRVLAIVVAAQFMFGVDSFIVNVAIPTISVELNASSSQLEAVIAIYLIGYATLIVTGGRLGDIYGT
KTVFLAGVIGFTLTSLWCGLARSGTELILARLAQGTTAALMVPQVLATLHVLFPDAARAKAFAIYGIVLGLAGAAGFALG
GLLVTLDLGGYGWRAIFFVNGPVGLIIFAAAAWVVPQAPRRPGTRLDLPGAVILFTGLLCVIGPLLFGREVDWAGWIWVV
MAAGVAIVLIFLRYERGVAARGGMPLIDLALLADSAFMRGLGAVFCFFFANQSFYLVVTLYMQMVLEIPPLPAGLVFLPL
ALAFVIAARHSGARARRRGTLVLIEGCLLQIAGLALVALTVAMIEAPTPFVLALVLIVVGYGQGLVMAPLSGAVLSTVQA
ASAGSGSGLYGTATQISSAAGIAAIGSLYFSLDHAISGQFAFLVALSVIAGSVAGSIILLHWMRRAALVPT

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=25.5980861244019, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1790195, Length=430, Percent_Identity=29.0697674418605, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788392, Length=419, Percent_Identity=26.9689737470167, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789042, Length=286, Percent_Identity=29.3706293706294, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788710, Length=310, Percent_Identity=28.3870967741935, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790146, Length=174, Percent_Identity=25.8620689655172, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48994; Mature: 48994

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50850 MFS ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTLAVTTASSRRWRVLAIVVAAQFMFGVDSFIVNVAIPTISVELNASSSQLEAVIAIY
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHH
LIGYATLIVTGGRLGDIYGTKTVFLAGVIGFTLTSLWCGLARSGTELILARLAQGTTAAL
HHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
MVPQVLATLHVLFPDAARAKAFAIYGIVLGLAGAAGFALGGLLVTLDLGGYGWRAIFFVN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEEC
GPVGLIIFAAAAWVVPQAPRRPGTRLDLPGAVILFTGLLCVIGPLLFGREVDWAGWIWVV
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
MAAGVAIVLIFLRYERGVAARGGMPLIDLALLADSAFMRGLGAVFCFFFANQSFYLVVTL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YMQMVLEIPPLPAGLVFLPLALAFVIAARHSGARARRRGTLVLIEGCLLQIAGLALVALT
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHH
VAMIEAPTPFVLALVLIVVGYGQGLVMAPLSGAVLSTVQAASAGSGSGLYGTATQISSAA
HHHHCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCEECHHHHHHHH
GIAAIGSLYFSLDHAISGQFAFLVALSVIAGSVAGSIILLHWMRRAALVPT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDQTLAVTTASSRRWRVLAIVVAAQFMFGVDSFIVNVAIPTISVELNASSSQLEAVIAIY
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHH
LIGYATLIVTGGRLGDIYGTKTVFLAGVIGFTLTSLWCGLARSGTELILARLAQGTTAAL
HHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
MVPQVLATLHVLFPDAARAKAFAIYGIVLGLAGAAGFALGGLLVTLDLGGYGWRAIFFVN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEEC
GPVGLIIFAAAAWVVPQAPRRPGTRLDLPGAVILFTGLLCVIGPLLFGREVDWAGWIWVV
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
MAAGVAIVLIFLRYERGVAARGGMPLIDLALLADSAFMRGLGAVFCFFFANQSFYLVVTL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YMQMVLEIPPLPAGLVFLPLALAFVIAARHSGARARRRGTLVLIEGCLLQIAGLALVALT
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEHHHHHHHHHHHHHHHH
VAMIEAPTPFVLALVLIVVGYGQGLVMAPLSGAVLSTVQAASAGSGSGLYGTATQISSAA
HHHHCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCEECHHHHHHHH
GIAAIGSLYFSLDHAISGQFAFLVALSVIAGSVAGSIILLHWMRRAALVPT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]