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The map label for this gene is phnD [H]

Identifier: 86751193

GI number: 86751193

Start: 4656508

End: 4657416

Strand: Direct

Name: phnD [H]

Synonym: RPB_4085

Alternate gene names: 86751193

Gene position: 4656508-4657416 (Clockwise)

Preceding gene: 86751192

Following gene: 86751194

Centisome position: 87.34

GC content: 59.96

Gene sequence:

>909_bases
ATGATCAAGTTCAGCACCGTTCTCGCAGGCGCCCTCGCGCTGGCGTTGACCGCCTCGGCCGCTCCGGCCCAGGATTGGAA
GTCGAAATATCCGGAACTGGTGTTCGGCAAGGTGCCGGACGAGAACGCCTCTGGCACCACCACCCGCTGGACGCCGCTGA
CGGAGTATCTGTCGAAGAAGCTCGGCACCAAGGTGACGCTGCGGATCGCCAACGACTATGCCGCCGTGATCGAAGGCCAG
CGCTCCGGGAACATCCAGATCGCGATGTACGGTCCGGCATCCTATGCCCGCGCCTATCTGATCGGCGCCAAGGTCGAACC
TTTCGCCATCGAAGTGAACAATGACGGCAGCAAAGGGTACTACTCGGTCCTCTACGTGAAGACGGAGTCGCCCTACAAGA
ACATTCAGGACCTGAAGGGCAAGAACCTTTGCCTGGTCGATCCGAATTCGACCTCGGGCAACAACGTCCCCCGCTTCGAG
ATGAACAAGCTGTCGATCGATCCGGACAAGTTCTTCGGCAAAGTGATTTACGCCGGCAGCCACGAGAATGCCGTGATCGG
CGTCGCGCAGGGCACCTGCGACGCCGCATTCAACTGGTGGAACACCGAAGCCGAATCCAACCTGCTGCGGATGGACCGCA
AGGGCATGGCCAAGGCGGCCGACTTCCGCATCATCCTGAAGTCCGACCAGATCGTGAATTCGCCGATGGCCTATCTCACC
GACCTGCCGGCGGACCTGAAGGCGGCGATCCGCAAGGCGGTTCTCGATCTGGCGACCGACGATCCGGAAACCTTCAACAA
GATCTACGAAGGCAAGGGCAAGCCTTATGACGCGGTCGATCACAAGGCCTATCAGCCGGTCATCGATCTGACGAAATTCG
TCGACACCCTGCGCAAGAGCAAATCCTGA

Upstream 100 bases:

>100_bases
CGGCGGCAAGAGCCGCCCCCGATCGCGCATCACCTCACCCTCGTTCGAAGCATCGTTCGAGGCTTGCCGTTCATGTCCAC
CCGAAAAGGCCCGTCACACC

Downstream 100 bases:

>100_bases
GCCGACACGATCGACTGAAACGGGCGGCCTTCAAGGCCGCCCTCTTTTTGCCAGGATAGTGGATGCCAGCGGTTGTTTCG
GAACTGCCCGACGCTCAGTT

Product: phosphonate-binding periplasmic protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MIKFSTVLAGALALALTASAAPAQDWKSKYPELVFGKVPDENASGTTTRWTPLTEYLSKKLGTKVTLRIANDYAAVIEGQ
RSGNIQIAMYGPASYARAYLIGAKVEPFAIEVNNDGSKGYYSVLYVKTESPYKNIQDLKGKNLCLVDPNSTSGNNVPRFE
MNKLSIDPDKFFGKVIYAGSHENAVIGVAQGTCDAAFNWWNTEAESNLLRMDRKGMAKAADFRIILKSDQIVNSPMAYLT
DLPADLKAAIRKAVLDLATDDPETFNKIYEGKGKPYDAVDHKAYQPVIDLTKFVDTLRKSKS

Sequences:

>Translated_302_residues
MIKFSTVLAGALALALTASAAPAQDWKSKYPELVFGKVPDENASGTTTRWTPLTEYLSKKLGTKVTLRIANDYAAVIEGQ
RSGNIQIAMYGPASYARAYLIGAKVEPFAIEVNNDGSKGYYSVLYVKTESPYKNIQDLKGKNLCLVDPNSTSGNNVPRFE
MNKLSIDPDKFFGKVIYAGSHENAVIGVAQGTCDAAFNWWNTEAESNLLRMDRKGMAKAADFRIILKSDQIVNSPMAYLT
DLPADLKAAIRKAVLDLATDDPETFNKIYEGKGKPYDAVDHKAYQPVIDLTKFVDTLRKSKS
>Mature_302_residues
MIKFSTVLAGALALALTASAAPAQDWKSKYPELVFGKVPDENASGTTTRWTPLTEYLSKKLGTKVTLRIANDYAAVIEGQ
RSGNIQIAMYGPASYARAYLIGAKVEPFAIEVNNDGSKGYYSVLYVKTESPYKNIQDLKGKNLCLVDPNSTSGNNVPRFE
MNKLSIDPDKFFGKVIYAGSHENAVIGVAQGTCDAAFNWWNTEAESNLLRMDRKGMAKAADFRIILKSDQIVNSPMAYLT
DLPADLKAAIRKAVLDLATDDPETFNKIYEGKGKPYDAVDHKAYQPVIDLTKFVDTLRKSKS

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for phosphate binding [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790543, Length=250, Percent_Identity=34.8, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 33172; Mature: 33172

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFSTVLAGALALALTASAAPAQDWKSKYPELVFGKVPDENASGTTTRWTPLTEYLSKK
CEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCCCCCCEEECCHHHHHHHHH
LGTKVTLRIANDYAAVIEGQRSGNIQIAMYGPASYARAYLIGAKVEPFAIEVNNDGSKGY
CCCEEEEEECCCEEEEEECCCCCCEEEEEECCHHHHEEEEECCCCCCEEEEECCCCCCCE
YSVLYVKTESPYKNIQDLKGKNLCLVDPNSTSGNNVPRFEMNKLSIDPDKFFGKVIYAGS
EEEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCCCCCEECCCCCCCHHHHCCEEEEECC
HENAVIGVAQGTCDAAFNWWNTEAESNLLRMDRKGMAKAADFRIILKSDQIVNSPMAYLT
CCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCHHHHH
DLPADLKAAIRKAVLDLATDDPETFNKIYEGKGKPYDAVDHKAYQPVIDLTKFVDTLRKS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
KS
CC
>Mature Secondary Structure
MIKFSTVLAGALALALTASAAPAQDWKSKYPELVFGKVPDENASGTTTRWTPLTEYLSKK
CEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCCCCCCEEECCHHHHHHHHH
LGTKVTLRIANDYAAVIEGQRSGNIQIAMYGPASYARAYLIGAKVEPFAIEVNNDGSKGY
CCCEEEEEECCCEEEEEECCCCCCEEEEEECCHHHHEEEEECCCCCCEEEEECCCCCCCE
YSVLYVKTESPYKNIQDLKGKNLCLVDPNSTSGNNVPRFEMNKLSIDPDKFFGKVIYAGS
EEEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCCCCCEECCCCCCCHHHHCCEEEEECC
HENAVIGVAQGTCDAAFNWWNTEAESNLLRMDRKGMAKAADFRIILKSDQIVNSPMAYLT
CCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCHHHHH
DLPADLKAAIRKAVLDLATDDPETFNKIYEGKGKPYDAVDHKAYQPVIDLTKFVDTLRKS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA