Click here to switch to the map view.

The map label for this gene is ilvX [H]

Identifier: 86751038

GI number: 86751038

Start: 4483569

End: 4485113

Strand: Reverse

Name: ilvX [H]

Synonym: RPB_3930

Alternate gene names: 86751038

Gene position: 4485113-4483569 (Counterclockwise)

Preceding gene: 86751041

Following gene: 86751037

Centisome position: 84.12

GC content: 70.03

Gene sequence:

>1545_bases
ATGAACGGTGCCGAAAGTCTGGTGCGGACGCTGGTCCATGGCGGCGTGGACGTCTGCTTCACCAATCCCGGCACCTCGGA
AATGCACTTCGTCGCCGCGCTCGACCGCGTCGAGGGCATGCGCTGCGTGCTCGGCCTGTTCGAGGGCGTGGTCACCGGCG
CCGCCGACGGTTATTTCCGGATGAAAGGCACGCCGGCCTCGACGCTGCTGCATCTCGGCCCCGGCCTCGCCAACGGCCTC
GCCAATCTGCACAACGCCAAGAAGGCGAGTTCCGGCATCGTCAACATCGTCGGCCAGCACGCCACCTACCACATCGACTA
CAACGCGCCGCTGACCTCCGACATCGAGGGCCTGGCGCGGCCGATGTCGGCCTGGGTCCGCACCTCGCCGGACGCGCAAT
CGGTGGCGCGCGACGGCGCCGCCGCGATTGCCGCCGCGAAGAGCGCGCCGCCGCAGATCGCCACCCTGATCCTGCCCGCC
GACACCGCCTGGGGCGAGGCCGACGGCATCGCCGAGGTGCCGCAAGACACCCAGCGCCCGAGCTATTCGCCGCACGCGGT
GGAAGCCGCGGCGCGCGTGCTGCGCTCCGGCGAGCCGACGCTGCTGCTGCTGACCGGCGGCGCGCTCACCGAACACGGCC
TCGAGCTCGCCGCGCGGATCGCCGGCAAGACCGGCTGCCGTGTGATGGGCCAGACCTACAATCCGCGGATGGCGCGCGGT
CGCGGCCGCTATGCGATCGAGCGGATTCCCTATGTGATCGAAGCCGCGCTGCCGATCCTGAAGGACTTCCGCCACATCGT
GCTGGTCGAGGCCAACGATCCGGTGGCGTTCTTCGCCTATCCGAACAAGCCGAGCCTGCTGAAACCGGACGGCTGCGAGG
TACATCGCATGACCGAGGGCGGCGAGAATTCCACCGCAGCGCTCGAAGCGCTGGCCGGCGCGCTCGGCGCCAAGGCGGCC
GACGCCCAGCCGCAGACCCATGTCGAGATCGCGCGGCCGAGCGGCGCGCTGACCCATGCCTCGATCGCCCAGGCGATCGC
GATGGCGATCCCGGACAACGCCATCGTGATCGACGAATCGATCACTACCGGCCGCGGCTTCTTTCCGCCGACGGCGGCGG
CGGCGCCGCACGACTGGCTGCAGAACATGGGCGGCTCGATCGGGTTCTCGCCGCCGGTCGCGGTCGGCGCCGCGGTGGCG
TGCCCGGATCGCAAGGTGATCTGCCTGGTCGGCGACGGCAGCGCGATGTACACGCTGCAGGCGCTGTGGACCCAGGCTCG
CGAAAATCTCGACGTCACCACCGTGGTGTTCGCCAACCGCAAATATCAGATCCTGCGCGGCGAGTTCGACGGCGTCGGCG
CCGGCGAGCCGGGCCAGCGCGCGCAGGACATGCTGTCGCTGGATCGGCCGAACCTCGACTGGGTGTCGCTGGCCCGGGGC
ATGGGCGTGCCGGCCCGCGCCGTGACCAGCGCCGATGAACTCAACAAGGCGCTCGACGCCGGCGTCGCCGGCAGCGGTCC
GAATTTGATCGAAGTGCAGATGTAG

Upstream 100 bases:

>100_bases
TGTCGCCTCGCGCAATTCTGATCCGCGCTGTGGCCCTTTGACGAAACCCCGGAACAATGATTGATCGGTCGAATAATCGT
CACAACCGGGGAGAGACGCG

Downstream 100 bases:

>100_bases
CCGGCGCGCCGGGCCGGCGGACGAAGCGGTTGTCGGACGCCCCGGCTCCGGCGTAGCCTGAGGGCCGGCGGCGCGGTTGC
CGCCGGTGGAGCAGAGATGA

Product: hypothetical protein

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MNGAESLVRTLVHGGVDVCFTNPGTSEMHFVAALDRVEGMRCVLGLFEGVVTGAADGYFRMKGTPASTLLHLGPGLANGL
ANLHNAKKASSGIVNIVGQHATYHIDYNAPLTSDIEGLARPMSAWVRTSPDAQSVARDGAAAIAAAKSAPPQIATLILPA
DTAWGEADGIAEVPQDTQRPSYSPHAVEAAARVLRSGEPTLLLLTGGALTEHGLELAARIAGKTGCRVMGQTYNPRMARG
RGRYAIERIPYVIEAALPILKDFRHIVLVEANDPVAFFAYPNKPSLLKPDGCEVHRMTEGGENSTAALEALAGALGAKAA
DAQPQTHVEIARPSGALTHASIAQAIAMAIPDNAIVIDESITTGRGFFPPTAAAAPHDWLQNMGGSIGFSPPVAVGAAVA
CPDRKVICLVGDGSAMYTLQALWTQARENLDVTTVVFANRKYQILRGEFDGVGAGEPGQRAQDMLSLDRPNLDWVSLARG
MGVPARAVTSADELNKALDAGVAGSGPNLIEVQM

Sequences:

>Translated_514_residues
MNGAESLVRTLVHGGVDVCFTNPGTSEMHFVAALDRVEGMRCVLGLFEGVVTGAADGYFRMKGTPASTLLHLGPGLANGL
ANLHNAKKASSGIVNIVGQHATYHIDYNAPLTSDIEGLARPMSAWVRTSPDAQSVARDGAAAIAAAKSAPPQIATLILPA
DTAWGEADGIAEVPQDTQRPSYSPHAVEAAARVLRSGEPTLLLLTGGALTEHGLELAARIAGKTGCRVMGQTYNPRMARG
RGRYAIERIPYVIEAALPILKDFRHIVLVEANDPVAFFAYPNKPSLLKPDGCEVHRMTEGGENSTAALEALAGALGAKAA
DAQPQTHVEIARPSGALTHASIAQAIAMAIPDNAIVIDESITTGRGFFPPTAAAAPHDWLQNMGGSIGFSPPVAVGAAVA
CPDRKVICLVGDGSAMYTLQALWTQARENLDVTTVVFANRKYQILRGEFDGVGAGEPGQRAQDMLSLDRPNLDWVSLARG
MGVPARAVTSADELNKALDAGVAGSGPNLIEVQM
>Mature_514_residues
MNGAESLVRTLVHGGVDVCFTNPGTSEMHFVAALDRVEGMRCVLGLFEGVVTGAADGYFRMKGTPASTLLHLGPGLANGL
ANLHNAKKASSGIVNIVGQHATYHIDYNAPLTSDIEGLARPMSAWVRTSPDAQSVARDGAAAIAAAKSAPPQIATLILPA
DTAWGEADGIAEVPQDTQRPSYSPHAVEAAARVLRSGEPTLLLLTGGALTEHGLELAARIAGKTGCRVMGQTYNPRMARG
RGRYAIERIPYVIEAALPILKDFRHIVLVEANDPVAFFAYPNKPSLLKPDGCEVHRMTEGGENSTAALEALAGALGAKAA
DAQPQTHVEIARPSGALTHASIAQAIAMAIPDNAIVIDESITTGRGFFPPTAAAAPHDWLQNMGGSIGFSPPVAVGAAVA
CPDRKVICLVGDGSAMYTLQALWTQARENLDVTTVVFANRKYQILRGEFDGVGAGEPGQRAQDMLSLDRPNLDWVSLARG
MGVPARAVTSADELNKALDAGVAGSGPNLIEVQM

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=25.4512635379061, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081685, Length=220, Percent_Identity=29.5454545454545, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 53704; Mature: 53704

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAESLVRTLVHGGVDVCFTNPGTSEMHFVAALDRVEGMRCVLGLFEGVVTGAADGYFR
CCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
MKGTPASTLLHLGPGLANGLANLHNAKKASSGIVNIVGQHATYHIDYNAPLTSDIEGLAR
ECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCHHHHHHHH
PMSAWVRTSPDAQSVARDGAAAIAAAKSAPPQIATLILPADTAWGEADGIAEVPQDTQRP
HHHHHHCCCCCHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
SYSPHAVEAAARVLRSGEPTLLLLTGGALTEHGLELAARIAGKTGCRVMGQTYNPRMARG
CCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHCC
RGRYAIERIPYVIEAALPILKDFRHIVLVEANDPVAFFAYPNKPSLLKPDGCEVHRMTEG
CCCCHHHHCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEECCC
GENSTAALEALAGALGAKAADAQPQTHVEIARPSGALTHASIAQAIAMAIPDNAIVIDES
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECC
ITTGRGFFPPTAAAAPHDWLQNMGGSIGFSPPVAVGAAVACPDRKVICLVGDGSAMYTLQ
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHCEEEECCCCEEEEEEECCCHHHHHH
ALWTQARENLDVTTVVFANRKYQILRGEFDGVGAGEPGQRAQDMLSLDRPNLDWVSLARG
HHHHHHHCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHC
MGVPARAVTSADELNKALDAGVAGSGPNLIEVQM
CCCCHHHHCCHHHHHHHHHCCCCCCCCCEEEEEC
>Mature Secondary Structure
MNGAESLVRTLVHGGVDVCFTNPGTSEMHFVAALDRVEGMRCVLGLFEGVVTGAADGYFR
CCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
MKGTPASTLLHLGPGLANGLANLHNAKKASSGIVNIVGQHATYHIDYNAPLTSDIEGLAR
ECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCHHHHHHHH
PMSAWVRTSPDAQSVARDGAAAIAAAKSAPPQIATLILPADTAWGEADGIAEVPQDTQRP
HHHHHHCCCCCHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
SYSPHAVEAAARVLRSGEPTLLLLTGGALTEHGLELAARIAGKTGCRVMGQTYNPRMARG
CCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHCC
RGRYAIERIPYVIEAALPILKDFRHIVLVEANDPVAFFAYPNKPSLLKPDGCEVHRMTEG
CCCCHHHHCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEECCC
GENSTAALEALAGALGAKAADAQPQTHVEIARPSGALTHASIAQAIAMAIPDNAIVIDES
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECC
ITTGRGFFPPTAAAAPHDWLQNMGGSIGFSPPVAVGAAVACPDRKVICLVGDGSAMYTLQ
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHCEEEECCCCEEEEEEECCCHHHHHH
ALWTQARENLDVTTVVFANRKYQILRGEFDGVGAGEPGQRAQDMLSLDRPNLDWVSLARG
HHHHHHHCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHC
MGVPARAVTSADELNKALDAGVAGSGPNLIEVQM
CCCCHHHHCCHHHHHHHHHCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]