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The map label for this gene is fumB [H]

Identifier: 86750871

GI number: 86750871

Start: 4296785

End: 4298452

Strand: Reverse

Name: fumB [H]

Synonym: RPB_3762

Alternate gene names: 86750871

Gene position: 4298452-4296785 (Counterclockwise)

Preceding gene: 86750872

Following gene: 86750868

Centisome position: 80.62

GC content: 65.83

Gene sequence:

>1668_bases
ATGAACGCACCCACGCCGATCCCGTCCGCCGCCACGCCGGTTCCGCCCTACACCCACACGCCGCTGTTTCCGCTCGGCAA
GGACGAGACGCCGTATCGCAAGATCACGTCGGAGGGGGTGCGTGTCGAGACGGTGCTGGGCCGGGACATGCTGGTGGTCG
AGCGCGAGGCGCTGCGGGCGCTGTCGGAGGCGGCGTTCGGCGACATCAACCATTATCTGCGGCCGGGCCATCTCGCCCAG
CTGCGCAAGATCCTCGACGATCCGGAGGCCAGCCCGAACGACAAGTTCGTGGCGCTGGACTTCCTGAAGAACGCCAACAT
CGCCGCCGGCGGCGTGCTGCCGATGTGCCAGGACACCGGCACCGCGATCATCATGGGCAAGAAGGGCTGCAACGTCATCA
CCGACGGCAGCGACGAGGCGGCGCTCTCCGAGGGCGCGCGCGATGCCTATCTGCGCCGCAATCTGCGCTACTCGCAGGTG
GCGCCGCTGACGATGTACGAAGAGAAGAACACCGCCAACAACATGCCGGCGCAGTGCGAGATCTACGCGGAAGGCCAAGG
GGACTCAGCAGCCGCCTACAAGTTCATGTTCATGGCCAAGGGCGGCGGCTCGGCCAACAAGAGCTTCCTGTTCCAGGCGA
CGCCGTCGGTGCTGACCCGCGACCGGCTGCTGGCGTTCCTGAAAGAGAAGATCATGACGCTCGGCACCGCGGCGTGCCCG
CCCTATCATCTGGCGATCGTGATCGGCGGCACCTCGGTCGAGTCGACCATGAAGACGGTGAAGCTCGCATCCGCGCGCTA
TCTCGACGCGCTGCCGACGCAAGGCTCCGAATTCGGCAACGCCTTCCGCGATCTCGAGATGGAGCAGGAAATCCTGAAGA
TGACGCAGAGCCTCGGCGTCGGCGCGCAGTTCGGCGGCAAGTATTTCTGCCACGACGTCCGAGTCATCCGGCTGCCGCGC
CACGGCGCGTCGTTGCCGATCGGGCTCGGCGTGTCGTGCTCGGCGGATCGCCAGGTGCTCGGCAAGATCACCAAGGACGG
CGTCTATCTCGAGGAGCTCGAGCACCATCCGGCGCAATATCTGCCGGAGGTCGAGCAGGCGCTCGGCGGCGAGGTGGTGC
AGATCGATCTGAACCAGCCGATGCAGGACATTCTCGCCACGCTGAGCGAACACCCGATCAAGACCCGGCTGTCGCTGACC
GGCACCATGATCGTGGCGCGCGACGCCGCCCATGCGAAATTGCGCGAACGCCTCGACAAGGGCGAGCCGTTGCCGGACTA
TTTCAAGAACCATCCGGTGTACTACGCCGGCCCCGCCAAGACGCCCGACGGCTACGCCTCCGGCGCGTTCGGCCCGACCA
CGGCGGGGCGGATGGACTCGTTCGTCGATCAGTTCCAGGCCGCCGGCGGCTCGATGGTGATGGTCGCCAAGGGCAACCGC
GCGCCCGCGGTGCGCGAGGCCTGCAAGAAATACGGCGGCTTCTATCTCGGCTCGATCGGCGGCGCCGCGGCGAACCTCGC
CGAGCACTGCATCAAGAAGGTCGAGGTGCTGGAATATCCCGAGCTCGGCATGGAGGCGATCTGGAAGATCGACGTCGTCG
ACTTCCCGGCCTTCATCATCGTCGACGACAAGGGCAACGACTTCTTCAAGGAACTCAATCTGGGCTGA

Upstream 100 bases:

>100_bases
TCTAGTTTCAGGATCTCCGAATTCGGTCCCGTTTTGAACCCTTCAAAACCTCTCCGAAACCGACTAGACAGGGCCGCAAA
TCCATCCTGAGGCCCCGCCC

Downstream 100 bases:

>100_bases
GGCGGCGCCAACAACCTCGCACCGTCATTCCGGGGCGCCGCGCAGCGGCGAACCCGGAATCCAGAGGTTGTTGCAGTCAG
GTGCTCAACAACTTCGAGAT

Product: fumarate hydratase, class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MNAPTPIPSAATPVPPYTHTPLFPLGKDETPYRKITSEGVRVETVLGRDMLVVEREALRALSEAAFGDINHYLRPGHLAQ
LRKILDDPEASPNDKFVALDFLKNANIAAGGVLPMCQDTGTAIIMGKKGCNVITDGSDEAALSEGARDAYLRRNLRYSQV
APLTMYEEKNTANNMPAQCEIYAEGQGDSAAAYKFMFMAKGGGSANKSFLFQATPSVLTRDRLLAFLKEKIMTLGTAACP
PYHLAIVIGGTSVESTMKTVKLASARYLDALPTQGSEFGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRVIRLPR
HGASLPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQYLPEVEQALGGEVVQIDLNQPMQDILATLSEHPIKTRLSLT
GTMIVARDAAHAKLRERLDKGEPLPDYFKNHPVYYAGPAKTPDGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNR
APAVREACKKYGGFYLGSIGGAAANLAEHCIKKVEVLEYPELGMEAIWKIDVVDFPAFIIVDDKGNDFFKELNLG

Sequences:

>Translated_555_residues
MNAPTPIPSAATPVPPYTHTPLFPLGKDETPYRKITSEGVRVETVLGRDMLVVEREALRALSEAAFGDINHYLRPGHLAQ
LRKILDDPEASPNDKFVALDFLKNANIAAGGVLPMCQDTGTAIIMGKKGCNVITDGSDEAALSEGARDAYLRRNLRYSQV
APLTMYEEKNTANNMPAQCEIYAEGQGDSAAAYKFMFMAKGGGSANKSFLFQATPSVLTRDRLLAFLKEKIMTLGTAACP
PYHLAIVIGGTSVESTMKTVKLASARYLDALPTQGSEFGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRVIRLPR
HGASLPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQYLPEVEQALGGEVVQIDLNQPMQDILATLSEHPIKTRLSLT
GTMIVARDAAHAKLRERLDKGEPLPDYFKNHPVYYAGPAKTPDGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNR
APAVREACKKYGGFYLGSIGGAAANLAEHCIKKVEVLEYPELGMEAIWKIDVVDFPAFIIVDDKGNDFFKELNLG
>Mature_555_residues
MNAPTPIPSAATPVPPYTHTPLFPLGKDETPYRKITSEGVRVETVLGRDMLVVEREALRALSEAAFGDINHYLRPGHLAQ
LRKILDDPEASPNDKFVALDFLKNANIAAGGVLPMCQDTGTAIIMGKKGCNVITDGSDEAALSEGARDAYLRRNLRYSQV
APLTMYEEKNTANNMPAQCEIYAEGQGDSAAAYKFMFMAKGGGSANKSFLFQATPSVLTRDRLLAFLKEKIMTLGTAACP
PYHLAIVIGGTSVESTMKTVKLASARYLDALPTQGSEFGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRVIRLPR
HGASLPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQYLPEVEQALGGEVVQIDLNQPMQDILATLSEHPIKTRLSLT
GTMIVARDAAHAKLRERLDKGEPLPDYFKNHPVYYAGPAKTPDGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNR
APAVREACKKYGGFYLGSIGGAAANLAEHCIKKVEVLEYPELGMEAIWKIDVVDFPAFIIVDDKGNDFFKELNLG

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=540, Percent_Identity=62.7777777777778, Blast_Score=705, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=538, Percent_Identity=61.8959107806691, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=159, Percent_Identity=31.4465408805031, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60144; Mature: 60144

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPTPIPSAATPVPPYTHTPLFPLGKDETPYRKITSEGVRVETVLGRDMLVVEREALRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCEEEEEHHHHHH
LSEAAFGDINHYLRPGHLAQLRKILDDPEASPNDKFVALDFLKNANIAAGGVLPMCQDTG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
TAIIMGKKGCNVITDGSDEAALSEGARDAYLRRNLRYSQVAPLTMYEEKNTANNMPAQCE
CEEEEECCCCCEEECCCCHHHHHCCHHHHHHHHCCCHHHCCCEEEECCCCCCCCCCCEEE
IYAEGQGDSAAAYKFMFMAKGGGSANKSFLFQATPSVLTRDRLLAFLKEKIMTLGTAACP
EEECCCCCCCEEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
PYHLAIVIGGTSVESTMKTVKLASARYLDALPTQGSEFGNAFRDLEMEQEILKMTQSLGV
CEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GAQFGGKYFCHDVRVIRLPRHGASLPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQY
CCCCCCEEEEECEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEHHHHHCCCHHH
LPEVEQALGGEVVQIDLNQPMQDILATLSEHPIKTRLSLTGTMIVARDAAHAKLRERLDK
HHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHHHHHHHHC
GEPLPDYFKNHPVYYAGPAKTPDGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNR
CCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
APAVREACKKYGGFYLGSIGGAAANLAEHCIKKVEVLEYPELGMEAIWKIDVVDFPAFII
CHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEECCCEEEE
VDDKGNDFFKELNLG
EECCCCHHHHHCCCC
>Mature Secondary Structure
MNAPTPIPSAATPVPPYTHTPLFPLGKDETPYRKITSEGVRVETVLGRDMLVVEREALRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCEEEEEHHHHHH
LSEAAFGDINHYLRPGHLAQLRKILDDPEASPNDKFVALDFLKNANIAAGGVLPMCQDTG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
TAIIMGKKGCNVITDGSDEAALSEGARDAYLRRNLRYSQVAPLTMYEEKNTANNMPAQCE
CEEEEECCCCCEEECCCCHHHHHCCHHHHHHHHCCCHHHCCCEEEECCCCCCCCCCCEEE
IYAEGQGDSAAAYKFMFMAKGGGSANKSFLFQATPSVLTRDRLLAFLKEKIMTLGTAACP
EEECCCCCCCEEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
PYHLAIVIGGTSVESTMKTVKLASARYLDALPTQGSEFGNAFRDLEMEQEILKMTQSLGV
CEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GAQFGGKYFCHDVRVIRLPRHGASLPIGLGVSCSADRQVLGKITKDGVYLEELEHHPAQY
CCCCCCEEEEECEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEHHHHHCCCHHH
LPEVEQALGGEVVQIDLNQPMQDILATLSEHPIKTRLSLTGTMIVARDAAHAKLRERLDK
HHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHHHHHHHHC
GEPLPDYFKNHPVYYAGPAKTPDGYASGAFGPTTAGRMDSFVDQFQAAGGSMVMVAKGNR
CCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
APAVREACKKYGGFYLGSIGGAAANLAEHCIKKVEVLEYPELGMEAIWKIDVVDFPAFII
CHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEECCCEEEE
VDDKGNDFFKELNLG
EECCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]