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The map label for this gene is groEL [H]

Identifier: 86750345

GI number: 86750345

Start: 3697445

End: 3699088

Strand: Direct

Name: groEL [H]

Synonym: RPB_3234

Alternate gene names: 86750345

Gene position: 3697445-3699088 (Clockwise)

Preceding gene: 86750344

Following gene: 86750346

Centisome position: 69.35

GC content: 65.51

Gene sequence:

>1644_bases
ATGTCCGCTAAAGAAGTCAAATTCGGCGTCGACGCCCGCGACCGCATGCTGCGCGGCGTGGACATTCTCGCCAATGCCGT
GAAGGTCACGCTCGGCCCGAAGGGCCGCAACGTCGTGCTCGACAAGTCGTTCGGCGCGCCCCGCATCACCAAGGACGGCG
TCACCGTCGCCAAGGAGATCGAGCTCGAGGACAAGTTCGAGAACATGGGCGCCCAGATGGTGCGCGAAGTGGCCTCGAAG
TCCGCCGATCTCGCCGGCGACGGCACCACTACCGCGACCGTGCTGGCCGCGGCGATCGTACGTGAAGGCGCCAAGTCGGT
GGCCGCCGGCATGAACCCGATGGATCTGAAGCGCGGCATCGACCTGGCTGTGGAAGCCGTGGTCGCCGACCTCGTCAAGA
ACTCCAAGAAGGTCACCTCGAACGACGAGATCGCCCAGGTCGGCACCATCTCGGCCAATGGCGACGCGGAAATCGGCAAG
TTCCTCGCCGACGCGATGAAGAAGGTCGGCAACGAGGGTGTCATCACCGTCGAGGAAGCCAAGTCGCTCGAGACCGAACT
CGACGTCGTCGAGGGCATGCAGTTCGACCGCGGCTACATCTCGCCCTACTTCGTCACCAACGCCGACAAGATGCGCGTCG
AATTCGACGACGCCTACATCCTGATCAACGAGAAGAAGCTCTCCAACCTCAACGAACTGCTGCCGCTGCTCGAAGCCGTG
GTGCAGACCGGCAAGCCGCTGGTGATCGTCGCTGAGGACGTCGAAGGCGAAGCGCTCGCCACCCTCGTCGTCAACCGCCT
GCGTGGCGGCCTGAAGGTCGCCGCCGTCAAGGCGCCGGGCTTCGGCGATCGCCGCAAGGCGATGCTGCAGGACATCGCGA
TCCTGACCGGCGGCCAGGCGATCTCGGAAGACCTCGGCATCAAGATGGAGAACGTCACGCTCCAGATGCTCGGTCGCGCC
AAGAAGGTGATGATCGACAAGGAGAACACCACGATCGTCAACGGCGCCGGTAAGAAGGTCGACATCGAAGCCCGCGTCGC
CCAGATCAAGGCGCAGATCGAGGAGACCACCTCGGACTACGATCGCGAGAAGCTGCAGGAGCGCCTGGCCAAGCTCGCCG
GCGGCGTCGCGGTGATCCGCGTCGGCGGCGCCACCGAGATCGAGGTCAAGGAGCGCAAGGATCGCGTTGACGACGCGATG
CACGCCACCCGCGCTGCGGTCGAGGAAGGCATCGTCCCGGGCGGCGGCGTCGCTCTGCTGCGCGCCTCCGAGCAGCTCAA
GCGCATCAAGACCGCGAACGACGACCAGAAGACCGGCGTCGAGATCGTGCGCAAGGCGCTCTCCGCCCCGGCCCGCCAGA
TCGCCATCAACGCCGGCGAAGACGGTTCGGTGATCGTCGGCAAGGTGCTGGAGAAGGATCAGTACAACTACGGCTTCGAC
AGCCAGACCGGCGAATACGGCGACATGGTCAAGAAGGGCATCATCGACCCGACCAAGGTCGTGCGTGCGGCGATCCAGAA
CGCGGCCTCGGTCGCGGCGCTCTTGATCACCACCGAAGCCATGATCGCGGAGCTGCCCAAGAAGGGCGGCGCCGGCGCCG
GCGGCATGCCCCCGGGCGGCGGCATGGGCGGCATGGATTTCTGA

Upstream 100 bases:

>100_bases
TGACGACCGACCACTGATCGTCCAACTCATCACCCTTCGAGGCTCGGCAACTTCGTTGCCCCGCACCTCAGGGTGACGCA
ACCAACCAAGGAAACCTCAT

Downstream 100 bases:

>100_bases
TCCAGCCACTCTCGCGCAAGCGAACTACGCAATGCAGAACCCCGGCGGAAACGCCGGGGTTTTTGTTTGGGGAATTGGCC
TGTAGCCCGGATGAAGCGAA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 2; Protein Cpn60 2 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MSAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEIELEDKFENMGAQMVREVASK
SADLAGDGTTTATVLAAAIVREGAKSVAAGMNPMDLKRGIDLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGK
FLADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAV
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKMENVTLQMLGRA
KKVMIDKENTTIVNGAGKKVDIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEIEVKERKDRVDDAM
HATRAAVEEGIVPGGGVALLRASEQLKRIKTANDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKVLEKDQYNYGFD
SQTGEYGDMVKKGIIDPTKVVRAAIQNAASVAALLITTEAMIAELPKKGGAGAGGMPPGGGMGGMDF

Sequences:

>Translated_547_residues
MSAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEIELEDKFENMGAQMVREVASK
SADLAGDGTTTATVLAAAIVREGAKSVAAGMNPMDLKRGIDLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGK
FLADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAV
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKMENVTLQMLGRA
KKVMIDKENTTIVNGAGKKVDIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEIEVKERKDRVDDAM
HATRAAVEEGIVPGGGVALLRASEQLKRIKTANDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKVLEKDQYNYGFD
SQTGEYGDMVKKGIIDPTKVVRAAIQNAASVAALLITTEAMIAELPKKGGAGAGGMPPGGGMGGMDF
>Mature_546_residues
SAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEIELEDKFENMGAQMVREVASKS
ADLAGDGTTTATVLAAAIVREGAKSVAAGMNPMDLKRGIDLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGKF
LADAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAVV
QTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQAISEDLGIKMENVTLQMLGRAK
KVMIDKENTTIVNGAGKKVDIEARVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEIEVKERKDRVDDAMH
ATRAAVEEGIVPGGGVALLRASEQLKRIKTANDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKVLEKDQYNYGFDS
QTGEYGDMVKKGIIDPTKVVRAAIQNAASVAALLITTEAMIAELPKKGGAGAGGMPPGGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=55.0284629981025, Blast_Score=583, Evalue=1e-166,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=55.0284629981025, Blast_Score=583, Evalue=1e-166,
Organism=Homo sapiens, GI5453607, Length=541, Percent_Identity=23.1053604436229, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1790586, Length=531, Percent_Identity=65.5367231638418, Blast_Score=691, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=527, Percent_Identity=55.0284629981025, Blast_Score=578, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI193210679, Length=238, Percent_Identity=50.8403361344538, Blast_Score=229, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=57.4144486692015, Blast_Score=606, Evalue=1e-174,
Organism=Saccharomyces cerevisiae, GI6322350, Length=545, Percent_Identity=23.8532110091743, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322524, Length=192, Percent_Identity=24.4791666666667, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=57.685009487666, Blast_Score=605, Evalue=1e-173,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=57.685009487666, Blast_Score=605, Evalue=1e-173,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=53.7142857142857, Blast_Score=571, Evalue=1e-163,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=53.7142857142857, Blast_Score=571, Evalue=1e-163,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=53.7142857142857, Blast_Score=571, Evalue=1e-163,
Organism=Drosophila melanogaster, GI17864606, Length=556, Percent_Identity=46.9424460431655, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24584129, Length=529, Percent_Identity=36.8620037807183, Blast_Score=345, Evalue=7e-95,
Organism=Drosophila melanogaster, GI19921262, Length=529, Percent_Identity=36.8620037807183, Blast_Score=345, Evalue=7e-95,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57928; Mature: 57797

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEI
CCCCCEEECCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEHHC
ELEDKFENMGAQMVREVASKSADLAGDGTTTATVLAAAIVREGAKSVAAGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGKFLADAMKKVGNEGVITVEEA
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAV
HHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCEEEEEECHHHCCHHHHHHHHHHH
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQA
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKMENVTLQMLGRAKKVMIDKENTTIVNGAGKKVDIEARVAQIKAQIEETTSDY
HHHHHCCEEHHHHHHHHCCCHHEEEECCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEIEVKERKDRVDDAMHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEH
RASEQLKRIKTANDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKVLEKDQYNYGFD
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHCCCCCCCCC
SQTGEYGDMVKKGIIDPTKVVRAAIQNAASVAALLITTEAMIAELPKKGGAGAGGMPPGG
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GMGGMDF
CCCCCCC
>Mature Secondary Structure 
SAKEVKFGVDARDRMLRGVDILANAVKVTLGPKGRNVVLDKSFGAPRITKDGVTVAKEI
CCCCEEECCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEHHC
ELEDKFENMGAQMVREVASKSADLAGDGTTTATVLAAAIVREGAKSVAAGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DLAVEAVVADLVKNSKKVTSNDEIAQVGTISANGDAEIGKFLADAMKKVGNEGVITVEEA
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHH
KSLETELDVVEGMQFDRGYISPYFVTNADKMRVEFDDAYILINEKKLSNLNELLPLLEAV
HHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCEEEEEECHHHCCHHHHHHHHHHH
VQTGKPLVIVAEDVEGEALATLVVNRLRGGLKVAAVKAPGFGDRRKAMLQDIAILTGGQA
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKMENVTLQMLGRAKKVMIDKENTTIVNGAGKKVDIEARVAQIKAQIEETTSDY
HHHHHCCEEHHHHHHHHCCCHHEEEECCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEIEVKERKDRVDDAMHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEH
RASEQLKRIKTANDDQKTGVEIVRKALSAPARQIAINAGEDGSVIVGKVLEKDQYNYGFD
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHCCCCCCCCC
SQTGEYGDMVKKGIIDPTKVVRAAIQNAASVAALLITTEAMIAELPKKGGAGAGGMPPGG
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GMGGMDF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8101485; 12597275 [H]