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The map label for this gene is hsrA [H]

Identifier: 86750306

GI number: 86750306

Start: 3653294

End: 3654766

Strand: Direct

Name: hsrA [H]

Synonym: RPB_3195

Alternate gene names: 86750306

Gene position: 3653294-3654766 (Clockwise)

Preceding gene: 86750299

Following gene: 86750309

Centisome position: 68.52

GC content: 67.41

Gene sequence:

>1473_bases
ATGAATAAAGAACGACTGATCCCGCTGATCGTGGCCGCCGCGCTGTTCATGGAGAACATGGACGCCACCGTGATCGCCAC
GTCATTGCCGGCGATTGCCGCCGATATCGGCACCTCGCCGCTGACGCTGAAGCTCGCGATCACGTCCTATCTGCTGTCGC
TGGCGGTGTTCATCCCGGCCTCCGGCTGGACCGCCGACCGGTTCGGCGCCCGCACGGTGTTCTCGGCGGCGATCGCGGTG
TTCATGATCGGCTCGATCGGCTGCGCGCTGTCGCAGTCGATCACCGATTTCGTGCTCGCGCGCATCCTGCAGGGGCTCGG
CGGCGCGATGATGACGCCGGTCGGACGATTGGTGCTGCTGCGCTCGATCGACAAGAGCGCACTGGTCGGCGCGATGGCGT
GGATGACGGTGCCGGCGCTGATCGGCCCGGTGATCGGCCCGCCGCTCGGCGGCTTCATCACCACCTACTTCTCGTGGCAC
TGGATCTTCCTGATCAACATCCCGATCGGGCTCGCCGGGATCTTCTTCGCGCAGCGCTACATCGATCCGATCCGCAGCGA
CAATCCGGAGCGGTTCGACCTCTACGGCCTGGTGCTGGCCGGCATCGGCCTCGCCGGCATCGCCTTCGGCCTGTCGGTGG
CGGGGCTCGGGCTGCTGCCCTGGCAGGTCGTCGTGGCGCTGATCGCGATCGGCACGATCGCGATGACGCTGTATCTGCTG
CACGCCCGCCGCACCGCCTCGCCGGTGCTGGATTTCTCGCTGCTGCGGCTGACGACGCTGCGCGCCAGCATGACCGGCGG
CTTCCTGTTCAGGCTCGGCATCGGCGCATTGCCGTTCCTGCTGCCGCTGTTGATGCAGATCGGCTTCGGGCTGTCGCCGT
TTCACTCCGGCCTCGTCACCTTCGCCTCCTCGGCCGGGGCGATGGGGATGAAGCCGCTGGCGGCGCGGATCATCCGCACC
TTCGGCTTCCGCAAGATCATGACCATCAACGCGCTGGTCAGCTCGGTGTTTCTCGCCGCCTGCGCGCTGTTCACGCCGGC
GACGCCGCTGCTGCTGATCATGATCATCCTGCTGGTCGGCGGCTTCTTCCGCTCGCTGCAATTCACCGCGATCAACACCG
TCGCCTATGCCGAGGTGGAGACCGCGCAGATGAGCCGCGCCACCACGCTGGTCAGCGTCGGCCAGCAGCTGGCGATCTCG
GCCGGCGTCGCGATCGGCGCGTTTGCGGTCGAATCCACGATGGCGTGGCACGGCACCACGACGCTCGGCGCCGACGATTT
CGCGCCGGCCTTCGTCGTGGTCGCGGTGCTGTCGGCGTTGTCGGCGTATTTCTTCTGGCGGATGCCGGACGATGCCGGCA
GCGAGATCTCGGGGCGGAAGGCGATCGAGATCTCGAGCCGGAAGGGCGCCGCCGGCGCCGCTGCCAAGACCGCGACCGAA
GAAACCCAGACCGCGCGCGATCAGCGACTGTGA

Upstream 100 bases:

>100_bases
GCGCCGGTCACGCAAGATTTAGGTGCGGGTGCGGCGTGCGCAATGGCGGCGGCTGCGACGCGATGCTACAGGCGGGCTCC
CTTCCCCGCCGAAACTCTGC

Downstream 100 bases:

>100_bases
CCGCCGCCGCGTGCTGGTGCAAGGCCGCCGCTGCCTGAGCTGGCCGGCGACTACGGTTCCTGCGGTTCCTGAGCCGCCGG
CTGTGTGCCGCGGAATCCCA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MNKERLIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPASGWTADRFGARTVFSAAIAV
FMIGSIGCALSQSITDFVLARILQGLGGAMMTPVGRLVLLRSIDKSALVGAMAWMTVPALIGPVIGPPLGGFITTYFSWH
WIFLINIPIGLAGIFFAQRYIDPIRSDNPERFDLYGLVLAGIGLAGIAFGLSVAGLGLLPWQVVVALIAIGTIAMTLYLL
HARRTASPVLDFSLLRLTTLRASMTGGFLFRLGIGALPFLLPLLMQIGFGLSPFHSGLVTFASSAGAMGMKPLAARIIRT
FGFRKIMTINALVSSVFLAACALFTPATPLLLIMIILLVGGFFRSLQFTAINTVAYAEVETAQMSRATTLVSVGQQLAIS
AGVAIGAFAVESTMAWHGTTTLGADDFAPAFVVVAVLSALSAYFFWRMPDDAGSEISGRKAIEISSRKGAAGAAAKTATE
ETQTARDQRL

Sequences:

>Translated_490_residues
MNKERLIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPASGWTADRFGARTVFSAAIAV
FMIGSIGCALSQSITDFVLARILQGLGGAMMTPVGRLVLLRSIDKSALVGAMAWMTVPALIGPVIGPPLGGFITTYFSWH
WIFLINIPIGLAGIFFAQRYIDPIRSDNPERFDLYGLVLAGIGLAGIAFGLSVAGLGLLPWQVVVALIAIGTIAMTLYLL
HARRTASPVLDFSLLRLTTLRASMTGGFLFRLGIGALPFLLPLLMQIGFGLSPFHSGLVTFASSAGAMGMKPLAARIIRT
FGFRKIMTINALVSSVFLAACALFTPATPLLLIMIILLVGGFFRSLQFTAINTVAYAEVETAQMSRATTLVSVGQQLAIS
AGVAIGAFAVESTMAWHGTTTLGADDFAPAFVVVAVLSALSAYFFWRMPDDAGSEISGRKAIEISSRKGAAGAAAKTATE
ETQTARDQRL
>Mature_490_residues
MNKERLIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPASGWTADRFGARTVFSAAIAV
FMIGSIGCALSQSITDFVLARILQGLGGAMMTPVGRLVLLRSIDKSALVGAMAWMTVPALIGPVIGPPLGGFITTYFSWH
WIFLINIPIGLAGIFFAQRYIDPIRSDNPERFDLYGLVLAGIGLAGIAFGLSVAGLGLLPWQVVVALIAIGTIAMTLYLL
HARRTASPVLDFSLLRLTTLRASMTGGFLFRLGIGALPFLLPLLMQIGFGLSPFHSGLVTFASSAGAMGMKPLAARIIRT
FGFRKIMTINALVSSVFLAACALFTPATPLLLIMIILLVGGFFRSLQFTAINTVAYAEVETAQMSRATTLVSVGQQLAIS
AGVAIGAFAVESTMAWHGTTTLGADDFAPAFVVVAVLSALSAYFFWRMPDDAGSEISGRKAIEISSRKGAAGAAAKTATE
ETQTARDQRL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=461, Percent_Identity=36.2255965292842, Blast_Score=320, Evalue=9e-89,
Organism=Escherichia coli, GI1788392, Length=472, Percent_Identity=36.6525423728814, Blast_Score=272, Evalue=5e-74,
Organism=Escherichia coli, GI1789042, Length=422, Percent_Identity=24.8815165876777, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788710, Length=429, Percent_Identity=24.9417249417249, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081983, Length=314, Percent_Identity=28.6624203821656, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790146, Length=169, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=252, Percent_Identity=26.1904761904762, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51664; Mature: 51664

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKERLIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPA
CCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
SGWTADRFGARTVFSAAIAVFMIGSIGCALSQSITDFVLARILQGLGGAMMTPVGRLVLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSIDKSALVGAMAWMTVPALIGPVIGPPLGGFITTYFSWHWIFLINIPIGLAGIFFAQRY
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHH
IDPIRSDNPERFDLYGLVLAGIGLAGIAFGLSVAGLGLLPWQVVVALIAIGTIAMTLYLL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
HARRTASPVLDFSLLRLTTLRASMTGGFLFRLGIGALPFLLPLLMQIGFGLSPFHSGLVT
HHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FASSAGAMGMKPLAARIIRTFGFRKIMTINALVSSVFLAACALFTPATPLLLIMIILLVG
HHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GFFRSLQFTAINTVAYAEVETAQMSRATTLVSVGQQLAISAGVAIGAFAVESTMAWHGTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEECCCC
TLGADDFAPAFVVVAVLSALSAYFFWRMPDDAGSEISGRKAIEISSRKGAAGAAAKTATE
CCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHH
ETQTARDQRL
HHHHHHHCCC
>Mature Secondary Structure
MNKERLIPLIVAAALFMENMDATVIATSLPAIAADIGTSPLTLKLAITSYLLSLAVFIPA
CCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
SGWTADRFGARTVFSAAIAVFMIGSIGCALSQSITDFVLARILQGLGGAMMTPVGRLVLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSIDKSALVGAMAWMTVPALIGPVIGPPLGGFITTYFSWHWIFLINIPIGLAGIFFAQRY
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHH
IDPIRSDNPERFDLYGLVLAGIGLAGIAFGLSVAGLGLLPWQVVVALIAIGTIAMTLYLL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
HARRTASPVLDFSLLRLTTLRASMTGGFLFRLGIGALPFLLPLLMQIGFGLSPFHSGLVT
HHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FASSAGAMGMKPLAARIIRTFGFRKIMTINALVSSVFLAACALFTPATPLLLIMIILLVG
HHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GFFRSLQFTAINTVAYAEVETAQMSRATTLVSVGQQLAISAGVAIGAFAVESTMAWHGTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEECCCC
TLGADDFAPAFVVVAVLSALSAYFFWRMPDDAGSEISGRKAIEISSRKGAAGAAAKTATE
CCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHH
ETQTARDQRL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]