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The map label for this gene is fixL [H]

Identifier: 86750263

GI number: 86750263

Start: 3605826

End: 3607247

Strand: Direct

Name: fixL [H]

Synonym: RPB_3152

Alternate gene names: 86750263

Gene position: 3605826-3607247 (Clockwise)

Preceding gene: 86750262

Following gene: 86750265

Centisome position: 67.63

GC content: 67.79

Gene sequence:

>1422_bases
ATGACCCGCAAACGATTCTCAAATTCAACTCTCGCGCCATGGCTGCTCGGCGCCACCCTGCTGCTGTTCATCGTCATCGC
CGGTGCGTTGATCCTGAATCTGATGCGGCTGCGCGACAGCTTCTCCTGGGTGCAGCACACCAACGACGCCCTGCTGGCGA
TTTCAGGCATCCAGCGCGAAGTGCTGGAAGCCGAGACCAACGAGCGCGGCTATCTGCTCACCGGCATCGACAGCTACCGC
GAGAATTACAACCATACGCGGGACACACTGGCATCGCGGCTCGACAGCCTGCGCTCGATCGTCACCGACAACCCCGAACA
TGTCGCCCGGGTCGACGATCTCCGCCAGTTGATCGACATGCGGACGGCCCAGCTCGGGCGGGTCATCGAACTGGGGCCGG
AGCGTGTGCGCGAGGCCCTCGACATTCTCGAGCAGGCCCGCACCGACCGGCTGACCGAACGCGTCGAAGCCAGCCTGAGC
GCCCTCACCCGTGTCGAACAAGCCCTGCTGACGGAGCGCATGTCGCGGCACGATCACGAGAGCCTCGCGGCGGCGCTGAT
CACCGCCTGCCTGCTGATCCTCGCCGTCGCCAGCGCCGCGATCGCCGCATTTCTGCTCGAGCACCAGCGCGCCGCGGCGC
GGCAGCAGGAGGCGGACCAGCGGCTGCAGAGCCTGCAGGCCGAATTGATGCGCGTGGCGCGGCTCAGCACCATGGGCGAG
ATGTCGAGCGCGCTGGCGCACGAGCTCAACCAGCCGCTCGGCGCGATCACCAACTACGTGCAGGGCTCGCGCCGGCTGGT
CGAGGCCAGCAGCCATCCCGACAAGGCGAAGATCGGCGCCGCGCTCGACAAGGCCGCGCAGCAGACGCTGCGCGCCGGTG
CGGTGATCCAGCGACTGCGCGAATTCGTCGGCCGCGGCGAGACCGACAAGACGGTCGAGAGCCTGCGCGCGATCGCCGAG
GACGCGCTGGCGCTCGCCTCCGTGGTCACGCGCGACCGCCCGGTCGACGTCGCGCTGACGCTCGATCCCGCGGTCGATCG
CGTGCTGGTCGACAAGGTGCAGGTGCAGCAGGTCTTCCTCAACCTGTTCCGCAACGCCTTCGAGGCGATGCACGAACTCC
CGGAACGGCTGCTCTCCATCACCAGCCGGGCCGTCGAGGACGGCATGATCGAGGTCGTGGTCGCCGATTCCGGTCCGGGT
CTCGATCCGCAGATCGCCGACCGGATGTTCCAGCCGTTCGAGACCACCAAAGCGGAAGGCATGGGGATCGGCCTGTCGAT
CTCGCAGACCATCATCCAGGCTCATGGCGGCTCGATCAACGCCGAGCCCGCCCCAGCCGGCGGCACGATGTTCCGTTTCA
CTTTGCCCTGCGCCGATCCCGGCGCACACGAGCCCCGCCCCGCCGCCGTCTCGGTGTTGTGA

Upstream 100 bases:

>100_bases
CCCTCCCGCTTCCGCCGCGGATGGCTGTCACCGCGGCGGTTCCAATTGAGCTCGGCGCGCGCGCGCTCTAGGGTGGCCAG
CCCAGCTCACAACAGTGCCG

Downstream 100 bases:

>100_bases
GCGGCGCGCCTATCCGACGCCCTGCTTCTGCATCCCGCCGATCGCTCCCACCCGCTCGGCGAAATGATCGTACAGTCCGG
CGAGTGCCGGCGGCGCAGCA

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTRKRFSNSTLAPWLLGATLLLFIVIAGALILNLMRLRDSFSWVQHTNDALLAISGIQREVLEAETNERGYLLTGIDSYR
ENYNHTRDTLASRLDSLRSIVTDNPEHVARVDDLRQLIDMRTAQLGRVIELGPERVREALDILEQARTDRLTERVEASLS
ALTRVEQALLTERMSRHDHESLAAALITACLLILAVASAAIAAFLLEHQRAAARQQEADQRLQSLQAELMRVARLSTMGE
MSSALAHELNQPLGAITNYVQGSRRLVEASSHPDKAKIGAALDKAAQQTLRAGAVIQRLREFVGRGETDKTVESLRAIAE
DALALASVVTRDRPVDVALTLDPAVDRVLVDKVQVQQVFLNLFRNAFEAMHELPERLLSITSRAVEDGMIEVVVADSGPG
LDPQIADRMFQPFETTKAEGMGIGLSISQTIIQAHGGSINAEPAPAGGTMFRFTLPCADPGAHEPRPAAVSVL

Sequences:

>Translated_473_residues
MTRKRFSNSTLAPWLLGATLLLFIVIAGALILNLMRLRDSFSWVQHTNDALLAISGIQREVLEAETNERGYLLTGIDSYR
ENYNHTRDTLASRLDSLRSIVTDNPEHVARVDDLRQLIDMRTAQLGRVIELGPERVREALDILEQARTDRLTERVEASLS
ALTRVEQALLTERMSRHDHESLAAALITACLLILAVASAAIAAFLLEHQRAAARQQEADQRLQSLQAELMRVARLSTMGE
MSSALAHELNQPLGAITNYVQGSRRLVEASSHPDKAKIGAALDKAAQQTLRAGAVIQRLREFVGRGETDKTVESLRAIAE
DALALASVVTRDRPVDVALTLDPAVDRVLVDKVQVQQVFLNLFRNAFEAMHELPERLLSITSRAVEDGMIEVVVADSGPG
LDPQIADRMFQPFETTKAEGMGIGLSISQTIIQAHGGSINAEPAPAGGTMFRFTLPCADPGAHEPRPAAVSVL
>Mature_472_residues
TRKRFSNSTLAPWLLGATLLLFIVIAGALILNLMRLRDSFSWVQHTNDALLAISGIQREVLEAETNERGYLLTGIDSYRE
NYNHTRDTLASRLDSLRSIVTDNPEHVARVDDLRQLIDMRTAQLGRVIELGPERVREALDILEQARTDRLTERVEASLSA
LTRVEQALLTERMSRHDHESLAAALITACLLILAVASAAIAAFLLEHQRAAARQQEADQRLQSLQAELMRVARLSTMGEM
SSALAHELNQPLGAITNYVQGSRRLVEASSHPDKAKIGAALDKAAQQTLRAGAVIQRLREFVGRGETDKTVESLRAIAED
ALALASVVTRDRPVDVALTLDPAVDRVLVDKVQVQQVFLNLFRNAFEAMHELPERLLSITSRAVEDGMIEVVVADSGPGL
DPQIADRMFQPFETTKAEGMGIGLSISQTIIQAHGGSINAEPAPAGGTMFRFTLPCADPGAHEPRPAAVSVL

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=236, Percent_Identity=31.3559322033898, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=28.0334728033473, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790300, Length=231, Percent_Identity=28.1385281385281, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87081816, Length=155, Percent_Identity=33.5483870967742, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786912, Length=124, Percent_Identity=33.8709677419355, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789149, Length=227, Percent_Identity=25.1101321585903, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51769; Mature: 51638

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKRFSNSTLAPWLLGATLLLFIVIAGALILNLMRLRDSFSWVQHTNDALLAISGIQRE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHH
VLEAETNERGYLLTGIDSYRENYNHTRDTLASRLDSLRSIVTDNPEHVARVDDLRQLIDM
HHHHCCCCCCEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RTAQLGRVIELGPERVREALDILEQARTDRLTERVEASLSALTRVEQALLTERMSRHDHE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SLAAALITACLLILAVASAAIAAFLLEHQRAAARQQEADQRLQSLQAELMRVARLSTMGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSSALAHELNQPLGAITNYVQGSRRLVEASSHPDKAKIGAALDKAAQQTLRAGAVIQRLR
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EFVGRGETDKTVESLRAIAEDALALASVVTRDRPVDVALTLDPAVDRVLVDKVQVQQVFL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
NLFRNAFEAMHELPERLLSITSRAVEDGMIEVVVADSGPGLDPQIADRMFQPFETTKAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHCCC
MGIGLSISQTIIQAHGGSINAEPAPAGGTMFRFTLPCADPGAHEPRPAAVSVL
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRKRFSNSTLAPWLLGATLLLFIVIAGALILNLMRLRDSFSWVQHTNDALLAISGIQRE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHH
VLEAETNERGYLLTGIDSYRENYNHTRDTLASRLDSLRSIVTDNPEHVARVDDLRQLIDM
HHHHCCCCCCEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RTAQLGRVIELGPERVREALDILEQARTDRLTERVEASLSALTRVEQALLTERMSRHDHE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SLAAALITACLLILAVASAAIAAFLLEHQRAAARQQEADQRLQSLQAELMRVARLSTMGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSSALAHELNQPLGAITNYVQGSRRLVEASSHPDKAKIGAALDKAAQQTLRAGAVIQRLR
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EFVGRGETDKTVESLRAIAEDALALASVVTRDRPVDVALTLDPAVDRVLVDKVQVQQVFL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
NLFRNAFEAMHELPERLLSITSRAVEDGMIEVVVADSGPGLDPQIADRMFQPFETTKAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHCCC
MGIGLSISQTIIQAHGGSINAEPAPAGGTMFRFTLPCADPGAHEPRPAAVSVL
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]