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The map label for this gene is aat

Identifier: 86750142

GI number: 86750142

Start: 3448365

End: 3449039

Strand: Direct

Name: aat

Synonym: RPB_3025

Alternate gene names: 86750142

Gene position: 3448365-3449039 (Clockwise)

Preceding gene: 86750141

Following gene: 86750144

Centisome position: 64.68

GC content: 67.26

Gene sequence:

>675_bases
ATGACATCCCGCGACTCCGCCGCCGCCGAGATCACCCCCGAAGTGCTGCTGCGCGCTTATGCCTGCGGCATCTTTCCGAT
GGCCGAAAGCGTCGACGATCCGACGCTGTTCTGGGTCGAGCCGGAATTGCGCGGCATCATTCCGCTCGGCGGCTTTCGCG
TCGCCTCCCGGCTCGCCCGCACGGTGCGGTCGGACGCTTTCACCGTCACCGTCAATCGCGACTTCAAGGGCGTGATCGAC
GGCTGCGCCGCGCCGCAGCCAGGCCGCGACGACACCTGGATCAACCGCCGGATTCGCGAGCTGTATATCGGCCTGCACGG
CATCGGCCATTGCCACAGCGTCGAGGTCTGGCAGGACGGCGACCTCGCCGGCGGTCTGTACGGCGTCAGCCTCGGCCGCG
CCTTCTTCGGCGAGAGCATGTTTCACCGCGCCCGCGACGCCTCCAAGGTGGCGCTGGTGCATCTCGTCGCCCGGTTGCTT
GCAGGCGGTTATGAACTGCTCGACACCCAGTTCGTCACCGACCATCTGCGCAGCTTCGGCGCAATCGAAGTCCCGCGGCA
ACGCTACCGTTCGATGCTCGACGACGCACTGCAGGGCATCGCCGCCTTCGATGCGCTGCCGGTGGATCAGCCCGTGACGG
GCGCCAAAGCGCTGGAGATCATCGCGGCGCACTGA

Upstream 100 bases:

>100_bases
GCTGGAATCCCGATCCGATCCGCTGACCGCGCTCCGGTCCCAATCGGCATTCGCTTCCGCCCCGGCTCGCGACCTCCGTC
CCGCCCTGCTATGCTCGGGC

Downstream 100 bases:

>100_bases
GCACGGCGATCAACGATCCCTCTGTCAGACCGGGAGCGGTTACTGCCGGAATGCCGGCAAAGGCGGCGGCGGCGCCTGCT
GGGTCTGGCGCGGTGGCGGC

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTSRDSAAAEITPEVLLRAYACGIFPMAESVDDPTLFWVEPELRGIIPLGGFRVASRLARTVRSDAFTVTVNRDFKGVID
GCAAPQPGRDDTWINRRIRELYIGLHGIGHCHSVEVWQDGDLAGGLYGVSLGRAFFGESMFHRARDASKVALVHLVARLL
AGGYELLDTQFVTDHLRSFGAIEVPRQRYRSMLDDALQGIAAFDALPVDQPVTGAKALEIIAAH

Sequences:

>Translated_224_residues
MTSRDSAAAEITPEVLLRAYACGIFPMAESVDDPTLFWVEPELRGIIPLGGFRVASRLARTVRSDAFTVTVNRDFKGVID
GCAAPQPGRDDTWINRRIRELYIGLHGIGHCHSVEVWQDGDLAGGLYGVSLGRAFFGESMFHRARDASKVALVHLVARLL
AGGYELLDTQFVTDHLRSFGAIEVPRQRYRSMLDDALQGIAAFDALPVDQPVTGAKALEIIAAH
>Mature_223_residues
TSRDSAAAEITPEVLLRAYACGIFPMAESVDDPTLFWVEPELRGIIPLGGFRVASRLARTVRSDAFTVTVNRDFKGVIDG
CAAPQPGRDDTWINRRIRELYIGLHGIGHCHSVEVWQDGDLAGGLYGVSLGRAFFGESMFHRARDASKVALVHLVARLLA
GGYELLDTQFVTDHLRSFGAIEVPRQRYRSMLDDALQGIAAFDALPVDQPVTGAKALEIIAAH

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=192, Percent_Identity=39.0625, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_RHOP2 (Q2IVN3)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486638.1
- ProteinModelPortal:   Q2IVN3
- SMR:   Q2IVN3
- STRING:   Q2IVN3
- GeneID:   3910824
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_3025
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- ProtClustDB:   PRK00301
- BioCyc:   RPAL316058:RPB_3025-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 24490; Mature: 24359

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRDSAAAEITPEVLLRAYACGIFPMAESVDDPTLFWVEPELRGIIPLGGFRVASRLAR
CCCCCCCHHHCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCEEECCCHHHHHHHHHH
TVRSDAFTVTVNRDFKGVIDGCAAPQPGRDDTWINRRIRELYIGLHGIGHCHSVEVWQDG
HHHCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
DLAGGLYGVSLGRAFFGESMFHRARDASKVALVHLVARLLAGGYELLDTQFVTDHLRSFG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
AIEVPRQRYRSMLDDALQGIAAFDALPVDQPVTGAKALEIIAAH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEECC
>Mature Secondary Structure 
TSRDSAAAEITPEVLLRAYACGIFPMAESVDDPTLFWVEPELRGIIPLGGFRVASRLAR
CCCCCCHHHCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCEEECCCHHHHHHHHHH
TVRSDAFTVTVNRDFKGVIDGCAAPQPGRDDTWINRRIRELYIGLHGIGHCHSVEVWQDG
HHHCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
DLAGGLYGVSLGRAFFGESMFHRARDASKVALVHLVARLLAGGYELLDTQFVTDHLRSFG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
AIEVPRQRYRSMLDDALQGIAAFDALPVDQPVTGAKALEIIAAH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA